E. coli S30 Extract System for Circular DNA from Promega

Product Specs
ItemE. coli S30 Extract System for Circular DNA
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL1020
Quantity30 reactions
Sample TypeE. coli cultures

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Systematic Evaluation of Different Ribonucleoprotein Complexes as Posttranscriptional Biosensors in Cell-Free TX-TL SystemsACS Synth BiolMay 5, 2026Dudu Boyvat, Francesco Grassi, Lucia Cassella, Velia Sicilianocomplete amino acid mixture, 20 μL of S30 Premix Without Amino Acids, 15 μL of S30 E. coli lysate (Promega L1020), and 0.5 μL of Endurazine (Promega), with the final volume adjusted to 50 μL using nuclease-free water.

(2) A dual-mechanism antimicrobial peptide with antimutagenic activity targets the replisome and induces cell envelope stressmSphereSeptember 30, 2025Amanda Holstad Singleton, Olaug Elisabeth Torheim Bergum, Jana Scheffold, Synnøve Brandt Ræder, Lilja Brekke Thorfinnsdottir, Lisa Marie Røst, Caroline Krogh Søgaard, Per Bruheim, Marit OtterleiBTP-001A, and R11 on translation were assessed using the E. coli extract system for circular DNA (Promega, L1020). The assay was performed according to the manufacturer’s instructions and as described in (17) with

(3) Synthetic macrolides overcoming MLSBK-resistant pathogensCell DiscoveryJuly 11, 2024Cong-Xuan Ma, Ye Li, Wen-Tian Liu, Yun Li, Fei Zhao, Xiao-Tian Lian, Jing Ding, Si-Meng Liu, Xie-Peng Liu, Bing-Zhi Fan, et al.in vitro transcription/translation assay for E. coli utilized materials from the E. coli S30 extract system for circular DNA (L1020, Promega, USA). This kit contains all the necessary components for efficient transcription and translation

(4) Artificial cell synthesis using biocatalytic polymerization-induced self-assemblyNature ChemistryApril 1, 2024Andrea Belluati, Sètuhn Jimaja, Robert J. Chadwick, Christopher Glynn, Mohamed Chami, Dominic Happel, Chao Guo, Harald Kolmar, Nico BrunsGUVs was achieved using the Promega E. coli S30 Extract Protein Expression System for Circular DNA (L1020). Seventy-five microlitres of plasmid, at different concentrations (pNCS–mClover3: 300 ng ml−1; ZP9A

(5) Proline-Hinged α-Helical Peptides Sensitize Gram-Positive Antibiotics, Expanding Their Physicochemical Properties to Be Used as Gram-Negative AntibioticsJournal of Medicinal ChemistryFebruary 8, 2024Yoonhwa Choi, Hyeong Woon Choe, Minsoo Kook, Seolah Choo, Tae Woo Park, Soeun Bae, Heeseung Kim, Jihye Yang, Woo-Seong Jeong, Jiyoung Yu, et al.concentration (IC50) of each antibiotic was measured with the E. coli S30 Extract System for Circular DNA kit (L1020, Promega). The experiment was performed as described in the manual with slight modifications. Briefly,

(6) Repurposing an Antioxidant to Kill Mycobacterium tuberculosis by Targeting the 50S Subunit of the RibosomeBiomoleculesDecember 14, 2023Wenqi Dong, Gaoyan Wang, Yajuan Bai, Yuxin Li, Liying Zhao, Wenjia Lu, Chenchen Wang, Zhaoran Zhang, Hao Lu, Xiangru Wang, et al.USA). A WST-1 cell proliferation and cytotoxicity assay kit and an E. coli S30 circular DNA assay kit (L1020) were purchased from Beyotime (Shanghai, China) and Promega (Wisconsin, DC, USA), respectively. Glycerol,

(7) Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosomePLOS BiologyMay 16, 2023Christopher E. Morgan, Yoon-Suk Kang, Alex B. Green, Kenneth P. Smith, Matthew G. Dowgiallo, Brandon C. Miller, Lucius Chiaraviglio, Katherine A. Truelson, Katelyn E. Zulauf, Shade Rodriguez, et al.NJ). For bacterial in vitro translation assays, E. coli S30 Extract System for Circular DNA (Promega, #L1020) and 100 ng of pCR XL TOPO-T7NLuc were then added to each reaction well, making use of the upstream

(8) NusG-mediated Coupling of Transcription and Translation Enhances Gene Expression by Suppressing RNA Polymerase BacktrackingJournal of Molecular BiologyJanuary 1, 2022Elizabeth J. Bailey, Max E. Gottesman, Ruben L. Gonzalezexpression and purification of luciferase-encoding mRNA The pBESTluc plasmid was obtained from Promega (No. L1020) and contains the eukaryotic firefly luciferase gene positioned downstream from a Ptac promoter and

(9) The anti-MRSA compound 3-O-alpha-L-(2”,3”-di-p-coumaroyl)rhamnoside (KCR) inhibits protein synthesis in Staphylococcus aureus.Journal of ProteomicsJanuary 1, 2020Nicholas J. Carruthers, Paul M. Stemmer, Joe Media, Ken Swartz, Xiaojuan Wang, Nicholas Aube, Mark T. Hamann, Frederick Valeriote, Jiajiu Shawa built-in FDR correction. Transcription/Translation Assay. E coli lysate from Promega (product # L1020, Madison, WI) was used to test the ability of KCR to inhibit transcription and translation of luciferase

(10) Comprehensive Profiling of Diverse Genetic Reporters with Application to Whole-Cell and Cell-Free BiosensorsAnalytical ChemistryDecember 3, 2019Antonia Lopreside, Xinyi Wan, Elisa Michelini, Aldo Roda, Baojun Wangin Vitro The cell-free reactions were performed using E. coli S30 Extract System for Circular DNA (L1020, Promega) according to the manufacturer’s protocol. Each reaction contained 40% (v/v) S30 premix, 10%

(11) An antimycobacterial pleuromutilin analogue effective against dormant bacilliBioorganic & Medicinal ChemistrySeptember 1, 2018Maddie R. Lemieux et al.assay Transcription/translation coupled assays were performed using commercially available E. coli S30 circular DNA assay kit (L1020) purchased from Promega Corporation. The assay used a control DNA template, pBESTluc™ vector containing

(12) Antimicrobial Activity, AME Resistance, and A-Site Binding Studies of Anthraquinone–Neomycin ConjugatesACS Infectious DiseasesMarch 10, 2017Natalya N. Degtyareva, Changjun Gong, Sandra Story, Nathanael S. Levinson, Adegboyega K. Oyelere, Keith D. Green, Sylvie Garneau-Tsodikova, Dev P. Aryaof test compounds in prokaryotic systems, the E. coli S30 Extract System for Circular DNA (Promega, L1020) was used. A Master Mix (MM) was created by combining 180 µL of S30 premix, 135 µL of S30 extract, 95

(13) Automated physics-based design of synthetic riboswitches from diverse RNA aptamersNucleic Acids ResearchJanuary 8, 2016Amin Espah Borujeni, Dennis M. Mishler, Jingzhi Wang, Walker Huso, Howard M. Salisluminescence measurements Cell-free transcription–translation assays were conducted using the S30 E. coli extract (Promega, L1020). TMR riboswitches were characterized by adding 20 ng/μl plasmid DNA to cell-free transcription–translation

(14) Exploiting translational stalling peptides in an effort to extend azithromycin interaction within the prokaryotic ribosome nascent peptide exit tunnelBioorganic & Medicinal ChemistryAugust 15, 2015Arren Z. Washington, Subhasish Tapadar, Alex George, Adegboyega K. OyelereμM for eukaryotic systems. Escherichia coli (E. coli S30 Extract System for Circular DNA, Promega, L1020) and rabbit reticulocyte (RRL system, nuclease treated, Promega, L4960) assays were performed as recommended

(15) Rapid Synthesis, RNA Binding, and Antibacterial Screening of a Peptidic-Aminosugar (PA) LibraryACS Chemical BiologyMay 15, 2015Liuwei Jiang, Derrick Watkins, Yi Jin, Changjun Gong, Ada King, Arren Z. Washington, Keith D. Green, Sylvie Garneau-Tsodikova, Adegboyega K. Oyelere, Dev P. AryanM for prokaryotic systems. Escherichia coli (E. coli S30 Extract System for Circular DNA, Promega, L1020) assays were performed as recommended by the manufacturer.59,60 Briefly, varying concentrations of the

(16) Paper-Based Synthetic Gene NetworksCellNovember 6, 2014Keith Pardee, Alexander A. Green, Tom Ferrante, D. Ewen Cameron, Ajay DaleyKeyser, Peng Yin, James J. Collinsmanufacturers and then immediately incubated at 37°C. Briefly, for S30 and S30T7 reactions (Promega, L1020 and L1110), cell extracts and premix containing amino acids were combined at a ratio of 0.33 and 0.51,

(17) A family of synthetic riboswitches adopts a kinetic trapping mechanismNucleic Acids ResearchJune 2, 2014Dennis M. Mishler, Justin P. GallivanIn vitro transcription and/or translation reactions were performed using S30 E. coli extract (Promega, item numbers L1020 and L1030). Reaction conditions followed those recommended by the manufacturer, and included S30 premix,

(18) Investigations of the Mode of Action and Resistance Development of Cadazolid, a New Antibiotic for Treatment of Clostridium difficile InfectionsAntimicrobial Agents and ChemotherapyFebruary 1, 2014Hans H. Locher, Patrick Caspers, Thierry Bruyère, Susanne Schroeder, Philippe Pfaff, Andreja Knezevic, Wolfgang Keck, Daniel Ritzcompound) 0.37 2–6 0.10 ⬍1.0 1.0 7.3 ⬎40 ⬍1.0 2.9 10.3 ⬎40 ⬎40 a E. coli S30 Extract System for Circular DNA (catalog no. L1020; Promega); E. coli RNA polymerase transcribing the luciferase gene from plasmid pBestluc. E. coli S30 Extract System for Circular DNA

(19) In Vitro and In Vivo Activities of Novel, Semisynthetic Thiopeptide Inhibitors of Bacterial Elongation Factor TuAntimicrobial Agents and ChemotherapyNovember 1, 2011J. A. Leeds et al.and translation assays. The Escherichia coli S30 extract system for circular DNA (Promega catalog no. L1020) was used according to the recommendations of the manufacturer with slight modifications. Reaction mixtures

(20) 4-Aminothiazolyl analogs of GE2270 A: Design, synthesis and evaluation of imidazole analogsBioorganic & Medicinal Chemistry LettersApril 12, 2011Matthew J. LaMarche, Jennifer A. Leeds, JoAnne Dzink-Fox, Steve Mullin, Michael A. Patane, Elin M. Rann, Stacey Tiamfook

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