TNT T7 Coupled Reticulocyte Lysate System from Promega

Product Specs
ItemTNT T7 Coupled Reticulocyte Lysate System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL4610
Quantity40 reactions
SystemT7 Coupled Reticulocyte

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Lycorine Derivative Inhibits SARS-CoV-2 Replication by Reducing -1 Programmed Ribosomal Frameshifting via Targeting ZAPMedComm (2020)April 6, 2026Tingfu Du, Ruixue Liu, Xintian Zhang, Longying Shen, Cong Tang, Junbin Wang, Yu Cheng, Wenhai Yu, Bin Yin, Shuaiyao Lu, et al.Assay This assay was conducted using the TNT T7 Coupled Reticulocyte Lysate Systems kit (Promega, L4610). In brief, the pcDNA4.0 SARS‐CoV‐2 reporter plasmid was constructed by inserting the frameshift sequence

(2) A C-terminal cytoplasmic retention motif and nuclear localization signal regulates nuclear import of TP53INP2/DORJ Cell SciDecember 10, 2025Birendra Kumar Shrestha, Eva Sjøttem, Hallvard Lauritz Olsvik, Isaac Odonkor, Aud Øvervatn, Hanne Britt Brenne, Jack-Ansgar Bruun, Trond Lamark, Terje Johansen35S-labeled proteins co-transcribed and translated using the TNT Coupled Reticulocyte Lysate System (Promega, L4610) as described previously (Pankiv et al., 2007). For quantifications, gels were vacuum dried and 35S-labeled

(3) p53 isoforms have a high aggregation propensity, interact with chaperones and lack binding to p53 interaction partnerseLifeSeptember 10, 2025Anamari Brdar, Christian Osterburg, Philipp Münick, Anne Christin Machel, Rajeshwari Rathore, Susanne Osterburg, Büşra Yüksel, Birgit Schäfer, Kristina Desch, Julian D Langer, et al.,Dual-Glo Luciferase Assay System,Promega,E2920, Commercial assay or kit,TnT Coupled Reticulocyte Lysate System,Promega,L4610, Commercial assay or kit,Trans-Blot Turbo RTA Midi PVDF Transfer Kit,Bio-Rad,#1704275, "Chemical compound,

(4) Targeting Mucoricin: A Novel Approach for Combating MucormycosisACS OmegaJuly 15, 2025Mansi Tanwar, Anamika Singh, Meetu Rathee, Chandra Prakash Prasad, Tej Pal Singh, Sujata Sharma, Pradeep Sharmadescribed previously using the Flexi Rabbit reticulocyte lysate system (Promega, Madison, WI, USA, L4610), mixed and incubated at 30 °C for 90 min, and the reaction was stopped by freezing at −20 °C. Luminescence

(5) Stem loop binding protein promotes SARS-CoV-2 replication via -1 programmed ribosomal frameshiftingSignal Transduct Target TherJune 13, 2025Tanxiu Chen, Ruimin Zhu, Tingfu Du, Hao Yang, Xintian Zhang, Zhixing Wang, Yong Zhang, Wenqi Quan, Bin Yin, Yunpeng Liu, et al.linearized DNA template was transcribed and translated with the TNT® T7 Coupled Reticulocyte Lysate System (L4610, Promega). Typical reactions were comprised of 12.5 μl TNT® Lysate, 1 μl TNT® Reaction Buffer, 0.5 μl

(6) Dual DNA demethylation mechanisms implement epigenetic memory driven by the pioneer factor PAX7Sci AdvMay 16, 2025Juliette Harris, Alexandre Mayran, Arthur Gouhier, Yves Gauthier, Nawal Hajj Sleiman, Samir Merabet, Michael Dukatz, Pavel Bashtrykov, Albert Jeltsch, Haig Djambazian, et al.described (22). 35S-labeled proteins were produced using either T7 TnT Coupled Reticulocyte Lysate Systems (L4610), T3 TnT Coupled Reticulocyte Lysate Systems (L4950), or TnT T7 Quick for PCR DNA (L5540) kits from

(7) Portimine A toxin causes skin inflammation through ZAKα-dependent NLRP1 inflammasome activationEMBO Molecular MedicineFebruary 13, 2025Léana Gorse, Loïc Plessis, Stephen Wearne, Margaux Paradis, Miriam Pinilla, Rae Chua, Seong Soo Lim, Elena Pelluz, Gee-Ann TOH, Raoul Mazars, et al.performed by using rabbit reticulocytes lysates with the TNT T7 Coupled Reticulocyte Lysate System (L4610; Promega) following the manufacturer’s recommendations. Translation was measured by the production of

(8) ETV2/ER71 regulates hematovascular lineage generation and vascularization through an H3K9 demethylase, KDM4AiScienceJanuary 17, 2025Min Seong Kim, Raham Lee, Dong Hun Lee, Heesang Song, Taekyung Ha, Joo Kyung Kim, Bum-Yong Kang, Karl Agger, Kristian Helin, Donghyun Shin, et al.LV900 Re-ChIP-IT Kit,Active Motif,Cat# 53016 TnT® coupled reticulocyte lysate systems (T7),Promega,Cat# L4610 ,, Deposited data,Deposited data,Deposited data ,, ChIP-seq data,NIH,https://www.ncbi.nlm.nih.gov/sra/PRJNA1051681 RNA-seq

(9) Chaperone-mediated insertion of mitochondrial import receptor TOM70 protects against diet-induced obesityNature Cell BiologyJanuary 3, 2025Pedro Latorre-Muro, Tevis Vitale, Matthew Ravichandran, Katherine Zhang, Jonathan M. Palozzi, Christopher F. Bennett, Arantza Lamas-Paz, Jee Hyung Sohn, Thomas D. Jackson, Mark Jedrychowski, et al.35S-labeled proteins were synthesized using the T7- based TnT rabbit reticulocyte system (Promega, L4610) following manufacturer’s instructions. Purified mitochondria (20-100 μg/time point) were pelleted and

(10) Cellular NS1-BP protein interacts with the mRNA export receptor NXF1 to mediate nuclear export of influenza virus M mRNAsJournal of Biological ChemistryNovember 1, 2024Ke Zhang, Tolga Cagatay, Dongqi Xie, Alexia E. Angelos, Serena Cornelius, Vasilisa Aksenova, Sadaf Aslam, Zhiyu He, Matthew Esparza, Ashley Vazhavilla, et al.reticulocyte lysate transcription–translation system according to the manufacturer’s instructions (Promega, L4610). 2 μM GST–NXT1•His–NXF1 recombinant protein complex or GST protein, 25 μl of either NUP98 or NUP153

(11) Eukaryotic release factor 1 from Euplotes promotes frameshifting at premature stop codons in human cellsiScienceApril 19, 2024Bozhidar-Adrian Stefanov, Elvis Ajuh, Sarah Allen, Mariusz NowackiNano-Glo® Dual-Luciferase® Reporter Assay System,Promega,N1610 Dual Luciferase Reporter Assay System,Promega,E1910 TnT coupled rabbit reticulocyte lysate T7,Promega,L4610 ,, Deposited data,Deposited data,Deposited data ,, Western blot images,Mendeley data,10.17632/6b6r2788ys.1 ,, Experimental models

(12) Identification and Biological Evaluation of a Novel Small-Molecule Inhibitor of Ricin ToxinMoleculesMarch 22, 2024Xinran Yang, Aili Wei, Xiyuan Cao, Zicheng Wang, Hongzhi Wan, Bo Wang, Hui Pengthe instructions provided for the TnT® Coupled Reticulocyte Lysate System (Promega, Madison, WI, USA, L4610). Different concentrations of RSMI-29 (100 and 200 μM) and RTA/ricin were added to the systems, then

(13) Anti–Peptidylarginine Deiminase 4 Autoantibodies and Disease Duration as Predictors of Treatment Response in Rheumatoid ArthritisACR Open RheumatologyFebruary 1, 2024Laura C. Cappelli, David Hines, Hong Wang, Clifton O. Bingham, Erika Darrahin vitro transcription and translation (IVTT) using the TNT Coupled Reticulocyte Lysate System (Promega, catalogue #L4610) according to the manufacturer's instructions. Immunoprecipitations were then performed in 96‐well round

(14) Recognition of polymorphic Csd proteins determines sex in the honeybeeScience AdvancesOctober 6, 2023Marianne Otte, Oksana Netschitailo, Stefanie Weidtkamp-Peters, Claus A. M. Seidel, Martin Beye,Thermo Fisher Scientific,Catalog no. FD0644 T7-transcription/translation coupled reticulocyte lysate system,Promega,Catalog no. L4610 Amersham ECL Kit,Thermo Fisher Scientific,Catalog no. 12316992 peqGOLD Tissue DNA Mini Kit,VWR,Catalog

(15) NgR1 binding to reovirus reveals an unusual bivalent interaction and a new viral attachment proteinProceedings of the National Academy of SciencesJune 13, 2023Danica M. Sutherland, Michael Strebl, Melanie Koehler, Olivia L. Welsh, Xinzhe Yu, Liya Hu, Rita dos Santos Natividade, Jonathan J. Knowlton, Gwen M. Taylor, Rodolfo A. Moreno, et al.translation reactions were conducted using the TNT-coupled rabbit reticulocyte lysate system (Promega, L4610) according to the manufacturer’s instructions. Reactions were supplemented with RNasin Plus RNase Inhibitor

(16) Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor controlScience advancesMay 12, 2023Schlienger S, Yam PT, Balekoglu N, Ducuing H, Michaud JF, Makihara S, Kramer DK, Chen B, Fasano A, Berardelli A, et al.in vitro transcription/translation (IVT) kit TnT Coupled Reticulocyte Lysate Systems (T7 Promoter) (Promega L4610) from pcDNA3-Human ARHGEF7WT-FLAG, pcDNA3-Human ARHGEF7mut-FLAG, and pcDNA3-Rat Git1WT-FLAG plasmids,

(17) Identification of an E3 ligase that targets the catalytic subunit of RNA Polymerase I upon transcription stressThe Journal of biological chemistryDecember 1, 2022Pitts S, Liu H, Ibrahim A, Garg A, Felgueira CM, Begum A, Fan W, Teh S, Low JY, Ford B, et al.were in vitro translated using the TNT T7 Coupled Reticulocyte Lysate System (Promega; catalog no.: L4610). The following plasmids were used: pCMV6-AC-RPA194-HA-His and pCMV6-FBXL14-Myc-DDK. Equal amounts of

(18) Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extractsCommunications BiologyOctober 28, 2022Lev Brio, Danit Wasserman, Efrat Michaely-Barbiro, Gal Barazany-Gal, Doron Gerber, Amit Tzurin-vitro expressed using rabbit reticulocyte lysate (TNT-coupled reticulocyte system; Promega; #L4600, #L4610) supplemented with either 35S-methionine/S-L-cysteine mix (PerkinElmer; #NEG772002MC) for radiography

(19) Targeting A-kinase anchoring protein 12 phosphorylation in hepatic stellate cells regulates liver injury and fibrosis in mouse modelseLifeOctober 4, 2022Ramani K, Mavila N, Abeynayake A, Tomasi ML, Wang J, Matsuda M, Seki E.plasmid Commercial assay or kit,Non-radioactive TNT Coupled Transcription/Translation system,"Promega, WI",Cat #L4610,In vitro translation Commercial assay or kit,Hydroxyproline Assay Kit,"Cell Biolabs Inc, CA",Cat #STA-675,Hydroxyproline

(20) A Drosophila Su(H) model of Adams-Oliver Syndrome reveals cofactor titration as a mechanism underlying developmental defectsPLOS GeneticsAugust 11, 2022Ellen K. Gagliani et al.scanner. In vitro protein translation The in vitro protein translations were performed using the TNT-assay (L4610) from Promega according to manufacturer’s instructions. Prior to EMSAs the in vitro translations of

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