TNT T7/T3 Coupled Reticulocyte Lysate System from Promega

Product Specs
ItemTNT T7/T3 Coupled Reticulocyte Lysate System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL5010
Quantity40 reactions
SystemT7/T3 Coupled Reticulocyte Lysate System
Molecular Weight~71kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) CBAP regulates the function of Akt-associated TSC protein complexes to modulate mTORC1 signalingThe Journal of biological chemistryDecember 1, 2023Liao WT, Chiang YJ, Yang-Yen HF, Hsu LC, Chang ZF, Yen JJY.Flag-TSC2 or myc-Akt1 protein was achieved using a TnT T7/T3 Coupled Reticulocyte System (Promega; L5010). Bacterial recombinant T7-Rhen (Pro-308) and His-Akt1 (PSPKA-068) were purchased from ProSpec. Protein

(2) Death-associated protein kinase 1 promotes growth of p53-mutant cancersThe Journal of clinical investigationJuly 1, 2015Zhao J, Zhao D, Poage GM, Mazumdar A, Zhang Y, Hill JL, Hartman ZC, Savage MI, Mills GB, Brown PH.for Western blot. In vitro kinase assay. TnT T7/T3 Coupled Reticulocyte Lysate System from Promega (L5010) was used to synthesize TSC2 protein according to the manufacturer’s protocol. TSC2 was immunoprecipitated

(3) Sox7 is regulated by ETV2 during cardiovascular developmentStem cells and developmentSeptember 1, 2014Behrens AN, Zierold C, Shi X, Ren Y, Koyano-Nakagawa N, Garry DJ, Martin CM.protein was in vitro translated by TNT-coupled transcription rabbit reticulocyte translation system (L5010; Promega). HA-ETV2 was incubated with 32P-labeled synthetic oligonucleotides containing putative EBE

(4) Measurement of protein farnesylation and geranylgeranylation in vitro, in cultured cells and in biopsies, and the effects of prenyl transferase inhibitorsNature protocolsOctober 27, 2011Berndt N, Sebti SM.(in vitro transcription-translation-prenylation) • TnT reticulocyte lysate system (Promega, cat. no. L4600, L4610, L4950, L5010, L5020) M CRITICAL Which system to purchase depends on the nature of the RNA polymerase promoter used

(5) Analysis of Thisbe and Pyramus functional domains reveals evidence for cleavage of Drosophila FGFsBMC developmental biologyAugust 5, 2010Tulin S, Stathopoulos A.labs). In vitro transcription/translation TnT T7/T3 Coupled Reticulocyte Lysate System (Promega, #L5010) was used with pBS-Pyr and pBS-Ths, incorporating S35 Methionine, to assay the unmodified, full-length

(6) Co-activator activator (CoAA) prevents the transcriptional activity of Runt domain transcription factorsJournal of Cellular BiochemistryOctober 1, 2009Xiaodong Li, Luke H. Hoeppner, Eric D. Jensen, Rajaram Gopalakrishnan, Jennifer J. WestendorfCoAM and CoAA-AxxQ were in vitro transcribed and translated with a T7 primer and the TNT kit (Promega, L5010) in the presence of 35S-methionine. GST or the indicated GST-Runx fusion proteins were isolated from

(7) CLOCK expression identifies developing circadian oscillator neurons in the brains of Drosophila embryosBMC NeuroscienceDecember 18, 2008Jerry H Houl, Fanny Ng, Pete Taylor, Paul E Hardinof plasmids containing the complete dac or Clk open reading frames was carried out using (Promega, L5010) as per manufacturer's instructions by combining the following: 25 μl TNT reticulocyte lysate; 2 μl

(8) Distinct in vitro interaction pattern of dopamine receptor subtypes with adaptor proteins involved in post-endocytotic receptor targetingFEBS LettersNovember 28, 2003Arne Heydorn, Birgitte P Søndergaard, Niels Hadrup, Birgitte Holst, Carol Renfrew Haft, Thue W Schwartz

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