E. coli S30 Extract System for Linear Templates from Promega

Product Specs
ItemE. coli S30 Extract System for Linear Templates
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL1030
Quantity30 reactions
Sample TypeE. coli cultures

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Commensal to pathogen switch in Streptococcus pneumoniae is influenced by a thermosensing master regulatorPLOS PathogensSeptember 30, 2025Shruti Apte, Greicy K. Bonifacio-Pereira, Sourav Ghosh, Srijit Kumar Mandal, Leena Badgujar, Krithika Gosavi, Elizabeth Pohler, Thomas E. Barton, Sian Pottenger, Alice Blake, et al.In vitro transcription/translation To in vitro transcribe and translate reporter GFP protein, S30 cell free extract system (Promega, L1030) was used as per manufacturer’s protocol. Briefly, ~ 4 µg of plasmid DNA (pAB414 containing PciaR-gfp

(2) Berberine analog of chloramphenicol exhibits a distinct mode of action and unveils ribosome plasticityStructureSeptember 1, 2024Zahra Batool, Julia A. Pavlova, Madhura N. Paranjpe, Andrey G. Tereshchenkov, Dmitrii A. Lukianov, Ilya A. Osterman, Alexey A. Bogdanov, Natalia V. Sumbatyan, Yury S. Polikanovkit,"New England Biolabs, USA",E6800 E. coli S30 Extract System for Linear Templates,"Promega, USA",L1030 Steady-Glo Luciferase Assay System,"Promega, USA",E2510 ,, Deposited data,Deposited data,Deposited

(3) AR cooperates with SMAD4 to maintain skeletal muscle homeostasisActa neuropathologicaJune 1, 2022Forouhan M, Lim WF, Zanetti-Domingues LC, Tynan CJ, Roberts TC, Malik B, Manzano R, Speciale AA, Ellerington R, Garcia-Guerra A, et al.coupled in vitro transcription/translation TNT wheat germ (Promega, L4130) and E. coli S30 (Promega, L1030) extract systems following the manufacturer’s protocols, respectively. PGEX-GST construct was used as

(4) A genetic selection reveals functional metastable structures embedded in a toxin-encoding mRNAeLifeAugust 14, 2019Sara Masachis, Nicolas J Tourasse, Claire Lays, Marion Faucher, Sandrine Chabas, Isabelle Iost, Fabien DarfeuillemRNAs, 0.5 µg of RNA was added to the E. coli S30 Extract System for Linear Templates Kit (Promega #L1030) as previously described (Sharma et al., 2010). In vitro structure probing 20 pmol of both aapA3-FL

(5) Mechanistic insights into type I toxin antitoxin systems inHelicobacter pylori:the importance of mRNA folding in controlling toxin expressionNucleic Acids ResearchJanuary 11, 2017Hélène Arnion, Dursun Nizam Korkut, Sara Masachis Gelo, Sandrine Chabas, Jérémy Reignier, Isabelle Iost, Fabien Darfeuilleof the AapA1_FL and AapA1_Tr1 mRNAs, 0.05 to 1 μg of RNA was added to the E. coli S30 kit (Promega, #L1030) as previously described (4). In vitro structure probing AapA1 transcripts were first dephosphorylated

(6) Mutually exclusive RNA secondary structures regulate translation initiation of DinQ in Escherichia coliRNANovember 1, 2016Knut I. Kristiansen, Ragnhild Weel-Sneve, James A. Booth, Magnar Bjøråsperformed with E. coli T7 S30 Extract System for Linear Templates according to protocol (Promega, catalog # L1030) with [14C]-leucine as radiolabeled amino acid. Briefly, 10 pmol of +1 or +44 dinQ RNA either alone

(7) Initiation of translation in bacteria by a structured eukaryotic IRES RNANatureMarch 5, 2015Timothy M. Colussi, David A. Costantino, Jianyu Zhu, John Paul Donohue, Andrei A. Korostelev, Zane A. Jaafar, Terra-Dawn M. Plank, Harry F. Noller, Jeffrey S. Kieftwater was added to a total final volume of 50 μL. For the reactions in E. coli lysate, Promega product #L1030 was used. 1 μL of labeled RNA was added to 15 μL of lysate and 20 μL of S30 premix supplemented with

(8) A family of synthetic riboswitches adopts a kinetic trapping mechanismNucleic Acids ResearchJune 2, 2014Dennis M. Mishler, Justin P. Gallivanand/or translation reactions were performed using S30 E. coli extract (Promega, item numbers L1020 and L1030). Reaction conditions followed those recommended by the manufacturer, and included S30 premix, S30 extract,

(9) Comparative Study between Transcriptionally- and Translationally-Acting Adenine Riboswitches Reveals Key Differences in Riboswitch Regulatory MechanismsPLoS GeneticsJanuary 20, 2011Jean-François Lemay, Guillaume Desnoyers, Simon Blouin, Benoit Heppell, Laurène Bastet, Patrick St-Pierre, Eric Massé, Daniel A. Lafontainetrials. In vitro translation assays Translation reactions were performed in E. coli S30 extracts (Promega, L1030). Coupled and uncoupled reactions were performed using either 25 nM of DNA or 250 nM RNA as template,

(10) An Antisense RNA Inhibits Translation by Competing with Standby RibosomesMolecular CellApril 5, 2007Fabien Darfeuille, Cecilia Unoson, Jörg Vogel, E. Gerhart H. Wagner

(11) Specific N-terminal biotinylation of a protein in vitro by a chemically modified tRNA fmet can support the native activity of the translated proteinJournal of Bioscience and BioengineeringMay 23, 2001Masumi Taki, Shinya Y. Sawata, Kazunari Taira

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