TNT SP6 Coupled Reticulocyte Lysate System from Promega

Product Specs
ItemTNT SP6 Coupled Reticulocyte Lysate System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL4600
Quantity40 reactions
SystemSP6 Coupled Reticulocyte Lysate

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Single-stranded DNA-binding proteins are essential components of the architectural LDB1 protein complexMolecular CellAugust 21, 2025Xiaokang Wang, Nicholas G. Aboreden, Ying Cai, Jessica C. Lam, Kate A. Henderson, Jiaqi Xiang, Belinda M. Giardine, Ross C. Hardison, Cheryl A. Keller, Lalitha Nagarajan, et al.Cell Line Nucleofector Kit (R),Lonza,Cat#VCA-1001 TnT® Coupled Reticulocyte Lysate Systems,Promega,Cat#L4600 Deposited data,Deposited data,Deposited data All ChIP-seq in this paper,This paper,GSE288078 All

(2) Transcription factor binding divergence drives transcriptional and phenotypic variation in maizeNature PlantsJune 12, 2025Mary Galli, Zongliang Chen, Tara Ghandour, Amina Chaudhry, Jason Gregory, Fan Feng, Miaomiao Li, Nathaniel Schleif, Xuan Zhang, Yinxin Dong, et al.plasmid DNA was used for in vitro protein expression using the TNT rabbit reticulocyte system (Promega L4600) per the manufacturer’s instructions. In vitro protein reactions were incubated for 2 hours at 30 degrees

(3) Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendageseLifeJanuary 30, 2025Tomoharu Kanie, Roy Ng, Keene L Abbott, Niaj Mohammad Tanvir, Esben Lorentzen, Olaf Pongs, Peter K JacksonpCS2-N-terminal 3×HA vectors described above using TnT Coupled Reticulocyte Lysate System under the SP6 promoter (L4600, Promega) and by following the manufacturer’s recommendations with few modifications. Briefly, instead

(4) Dbi1 is an oxidoreductase and an assembly chaperone for mitochondrial inner membrane proteinsEMBO ReportsJanuary 3, 2025Soraya Badrie, Kai Hell, Dejana MokranjacCL-4B,Cytiva,17096303 Proteinase K (PK),Roche Diagnostics,3115836001 TNT® Coupled Reticulocyte Lysate,Promega,L4600 Trichloroacetic acid (TCA),Sigma-Aldrich,T6399 Tris(2-carboxyethyl)phosphine (TCEP),Sigma-Aldrich,C4706 Triton-X100,Sigma-Aldrich,X100 Yeast

(5) Epigenomic identification of vernalization cis-regulatory elements in winter wheatGenome BiologyJuly 30, 2024Yanhong Liu, Pan Liu, Lifeng Gao, Yushan Li, Xueni Ren, Jizeng Jia, Lei Wang, Xu Zheng, Yiping Tong, Hongcui Pei, et al.and HALO-SPL15B protein were generated using the TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600) according to the manufacturer’s protocol. The protein was then incubated with Magne-HALO Tag beads

(6) Dry molten globule conformational state of CYP11A1 (SCC) regulates the first step of steroidogenesis in the mitochondrial matrixiScienceJune 21, 2024Himangshu S. BoseBiomedicals,07170102 Quick Start Bradford 1X Dye,BIO-RAD,5000205 Rabbit Reticulocyte Lysate,Promega,L4600 Protease Inhibitor Cocktail Set,Calbiochem,539132 Chemiluminescent Reagent,Thermo Fisher,34579 pSP64

(7) Gankyrin inhibits ferroptosis through the p53/SLC7A11/GPX4 axis in triple-negative breast cancer cellsSci RepDecember 8, 2023Ming Lei, Yun-Long Zhang, Feng-Ying Huang, Heng-Yu Chen, Ming-Hui Chen, Ri-Hong Wu, Shu-Zhen Dai, Gui-Sheng He, Guang-Hong Tan, Wu-Ping Zhengtreatment with DNase and RNase A. MDM2 protein was obtained from a rabbit reticulocyte lysate system (L4600, Promega, WI) and 35S-labeled methionine (JW-S53290, GIVEI, Shanghai, China) was added to the translation

(8) Double DAP-seq uncovered synergistic DNA binding of interacting bZIP transcription factorsNature CommunicationsMay 5, 2023Miaomiao Li, Tao Yao, Wanru Lin, Will E. Hinckley, Mary Galli, Wellington Muchero, Andrea Gallavotti, Jin-Gui Chen, Shao-shan Carol Huang53, 9, 10, 25, and 63) were expressed using the TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600). Expression of Halo-bZIP and SBP-bZIP proteins were confirmed by western blotting using anti-HaloTag

(9) RETRACTED: UPL5 modulates WHY2 protein distribution in a Kub-site dependent ubiquitination in response to [Ca2+]cyt-induced leaf senescenceiScienceMarch 17, 2023Wei Lan, Weibo Ma, Shuai Zheng, Ping Yang, Yuhao Qiu, Wenfang Lin, Yujun Ren, Ying Miaodetection,10662-310,Amersham Quik Change site-directed mutagenesis kit,200523,Agilent TnT coupled reticulocyte lysate systems,L4600,Promega ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, PQE31,"Miao and Zentgraf, 201037",Ying

(10) Mechanistic Insights into the Role of OPN in Mediating Brain Damage via Triggering Lysosomal Damage in Microglia/MacrophageCellsMarch 9, 2023Gai C, Zhao Y, Xin D, Li T, Cheng Y, Jiang Z, Song Y, Liu D, Wang Z.were generated in vitro through translation using TnT® T7 Coupled Reticulocyte Lysate Systems (Cat# L4600, Madison, Promega, WI, USA) according to the manufacturer’s protocol. The same analysis as that described

(11) Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendagesbioRxiv : the preprint server for biologyJanuary 10, 2023Kanie T, Ng R, Abbott KL, Pongs O, Jackson PK.3×HA vectors described above using TnT® Coupled Reticulocyte Lysate System under the SP6 promoter (L4600, Promega) and by following manufacturer’s recommendations with few modifications. Briefly, instead of

(12) Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extractsCommunications BiologyOctober 28, 2022Lev Brio, Danit Wasserman, Efrat Michaely-Barbiro, Gal Barazany-Gal, Doron Gerber, Amit Tzurwere in-vitro expressed using rabbit reticulocyte lysate (TNT-coupled reticulocyte system; Promega; #L4600, #L4610) supplemented with either 35S-methionine/S-L-cysteine mix (PerkinElmer; #NEG772002MC) for radiography

(13) Revisiting the multisite phosphorylation that produces the M-phase supershift of key mitotic regulatorsMolecular biology of the cellOctober 1, 2022Tan T, Wu C, Liu B, Pan BF, Hawke DH, Su Z, Liu S, Zhang W, Wang R, Lin SH, et al.Quick Coupled Transcription/Translation System (L2080) or TnT Coupled Transcription/Translation System (L4600) obtained from Promega Corporation following the instruction manual. The TNT products were either used

(14) PERK prevents hepatic lipotoxicity by activating the p62-ULK1 axis-mediated noncanonical KEAP1-Nrf2 pathwayRedox biologyApril 1, 2022Lee DH, Park JS, Lee YS, Bae SH.was prepared via in vitro translation using a TNT-coupled SP6 reticulocyte lysate system (Promega, L4600). Pull-down assays were performed by incubating equal quantities of GST or GST‐p62, and ULK1 fusion

(15) Regulation of the endocytosis and prion-chaperoning machineries by yeast E3 ubiquitin ligase Rsp5 as revealed by orthogonal ubiquitin transferCell chemical biologySeptember 16, 2021Wang Y, Fang S, Chen G, Ganti R, Chernova TA, Zhou L, Duong D, Kiyokawa H, Li M, Zhao B, et al.Chemiluminescent Substrate,Thermo Fisher Scientific,Cat#34580 TNT-coupled rabbit reticulocyte kit,Promega,Cat#L4600 Deposited Data,Deposited Data,Deposited Data MS proteomics data of HBT-xUB-conjugated proteins from

(16) Phosphorylation of SARS-CoV-2 Orf9b Regulates Its Targeting to Two Binding Sites in TOM70 and Recruitment of Hsp90International Journal of Molecular SciencesAugust 26, 2021Lukas Brandherm, Antonio Mario Kobaš, Mara Klöhn, Yannick Brüggemann, Stephanie Pfaender, Joachim Rassow, Sebastian Kreimendahltranslation in commercially available reticulocyte lysate (TNT Coupled Reticulocyte Lysate System, L4600; Promega, Madison, WI, USA) in the presence of [35S]-methionine (added from a stock solution of 10 μCi/μL,

(17) A small molecule that induces translational readthrough of CFTR nonsense mutations by eRF1 depletionNature communicationsJuly 16, 2021Sharma J, Du M, Wong E, Mutyam V, Li Y, Chen J, Wangen J, Thrasher K, Fu L, Peng N, et al.In vitro transcription/translation assay The TnT® SP6 Coupled Reticulocyte Lysate System (Promega, cat#: L4600) was used to generate the protein products expressed from the QXQ readthrough plasmid (pDB603)41 which

(18) Elucidating Human Mitosis Using an Anaphase-Like Cell-Free SystemMethods in molecular biology (Clifton, N.J.)January 1, 2021Wasserman D, Nachum S, Noach-Hirsh M, Auerbach N, Sheinberger-Chorni E, Enrico TP, Lahmi R, Emanuele MJ, Tzur A.TNT® SP6 coupled reticulocyte lysate system. Protein translation was satisfactory in both premixed and non-premixed lysate systems (Promega; #L4600). Expression by T3 or T7 promoters is possible by matching TNT® systems from Promega. Degradation

(19) The selectivity filter of the mitochondrial protein import machineryBMC BiologyDecember 1, 2020Sebastian Kreimendahl, Jan Schwichtenberg, Kathrin Günnewig, Lukas Brandherm, Joachim Rassowtranslation in commercially available reticulocyte lysate (TNT Coupled Reticulocyte Lysate System, Promega, L4600) in the presence of [35S]-methionine (added from a stock solution of 10 μCi/μl, Hartmann Analytic, SCM01/37)

(20) Cell cycle oscillators underlying orderly proteolysis of E2F8Molecular biology of the cellApril 1, 2020Wasserman D, Nachum S, Cohen M, Enrico TP, Noach-Hirsh M, Parasol J, Zomer-Polak S, Auerbach N, Sheinberger-Chorni E, Nevenzal H, et al.transcribed and translated in rabbit reticulocyte lysate (TNT-coupled reticulocyte system; Promega; #L4600, #L4610) supplemented with 35S-methionine (IsoLabel L-35S Steady Blue, Izotop; #TSM-01). Degradation/mobility-shift assays

Be the First to Write a Review!