TNT T7 Coupled Reticulocyte Lysate System, Trial Size from Promega

Product Specs
ItemTNT T7 Coupled Reticulocyte Lysate System, Trial Size
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL4611
Quantity8 reactions
SystemT7 Coupled Reticulocyte
Molecular Weight~59kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Short internal open reading frames repress the translation of N-terminally truncated proteoformsEMBO ReportsFebruary 17, 2025Raphael Fettig, Zita Gonda, Niklas Walter, Paul Sallmann, Christiane Thanisch, Markus Winter, Susanne Bauer, Lei Zhang, Greta Linden, Margarethe Litfin, et al.2000",https://emboss.sourceforge.net Other,Other,Other T7 TNT Coupled Reticulocyte Lysate System,Promega,Cat# L4611 QuikChange site-directed mutagenesis kit,Agilent,Cat# 200513 Zero Blunt TOPO PCR cloning kit,ThermoFisher Scientific

(2) Identification of protease serine S1 family member 53 as a mitochondrial protein in murine islet beta cellsIsletsDecember 31, 2022Noriko Mizusawa, Nagakatsu Harada, Takeo Iwata, Izumi Ohigashi, Mitsuo Itakura, Katsuhiko Yoshimotocontrol of the T7 promoter using a transcription–translation coupled reticulocyte lysate system (Promega; L4611) with pCR-Blunt II-Prss53 cDNA (including a stop codon) or pcDNA3.1/myc-His-Prss53 cDNA at 30°C for

(3) In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate ScreeningJournal of Visualized ExperimentsOctober 25, 2022Prathibha Yarikipati, Andreas BergmannScientific,14–959-49A, Sucrose,Sigma-Aldrich,S70903–250G, TnT Coupled Reticulocyte Lysate -T7,Promega,L4611, Tris-base,Fisher Scientific,BP154–1, Tween 20,Sigma-Aldrich,P1379, Waterbath,Fisher Scientific,2340, Western

(4) Acute sleep deprivation upregulates serotonin 2A receptors in the frontal cortex of mice via the immediate early gene Egr3Molecular PsychiatryMarch 1, 2022Xiuli Zhao et al.recombinant EGR3 protein was synthesized using the TnT Coupled Reticulocyte Lysate System (Promega, Cat. # L4611) using template pcDNA 3.1(−) mouse Egr3, a gift from Peter Johnson (Addgene plasmid # 107998; http://n2t.net/addgene:107998;

(5) The kinesin KIF4 mediates HBV/HDV entry through the regulation of surface NTCP localization and can be targeted by RXR agonists in vitroPLoS pathogensMarch 1, 2022Gad SA, Sugiyama M, Tsuge M, Wakae K, Fukano K, Oshima M, Sureau C, Watanabe N, Kato T, Murayama A, et al.translated from pIRESpuro3 expression vector using the TNT Coupled Reticulocyte Lysate Systems (Promega, L4611) following manufacturer’s recommendation; the recombinant NTCP protein preparation was previously reported

(6) C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assemblyCell metabolismMarch 2, 2021Wang T, Liu H, Itoh K, Oh S, Zhao L, Murata D, Sesaki H, Hartung T, Na CH, Wang J.,New England Biolabs,E5510S Cell counting kit-8 (CCK-8),Abcam,Ab228554 TNT coupled reticulocyte lysate systems,Promega,L4611 Complex I enzyme activity microplate assay kit,Abcam,ab109721 Mitochondrial Complex I activity colorimetric assay kit

(7) RNAi Screening Identifies that TEX10 Promotes the Proliferation of Colorectal Cancer Cells by Increasing NF-κB ActivationAdvanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2020Wang Z, Sheng C, Kan G, Yao C, Geng R, Chen S.Coupled Reticulocyte Lysate Systems according to the manufacturer's instructions (Promega, Madison, USA, #L4611). The in vitro translated Myc‐TEX10 was incubated with GST proteins overnight and analyzed by IB. siRNA

(8) Convallatoxin-Induced Reduction of Methionine Import Effectively Inhibits Human Cytomegalovirus Infection and ReplicationJournal of VirologyDecember 1, 2016Tobias Cohen, John D. Williams, Timothy J. Opperman, Roberto Sanchez, Nell S. Lurain, Domenico Tortorellainhibition was determined using the TnT T7 Coupled Reticulocyte Lysate system (Promega; catalog no. L4611) according to the manufacturer’s protocol for luciferase control experiments, with the following modifications.

(9) NOD1 Participates in the Innate Immune Response Triggered by Hepatitis C Virus PolymeraseJournal of VirologyJuly 1, 2016Serena Vegna, Damien Gregoire, Marie Moreau, Patrice Lassus, David Durantel, Eric Assenat, Urszula Hibner, Yannick Simoninwith [35S]methionine (PerkinElmer) was synthetized in a separate reaction with a TNT coupled reticulocyte lysate kit (L4611; Promega) according to the manufacturer’s instructions. Fifteen microliters of extracts was mixed with

(10) Corp Regulates P53 in Drosophila melanogaster via a Negative Feedback LoopPLoS geneticsJuly 1, 2015Chakraborty R, Li Y, Zhou L, Golic KG.synthesis were carried out using the TnT Coupled Reticulocyte Lysate Systems (Promega, Catalog number L4611) following manufacturer’s instructions. GST pull-down assays For in vitro synthesized protein, 1 ug

(11) Wedelolactone disrupts the interaction of EZH2-EED complex and inhibits PRC2-dependent cancerOncotargetMay 30, 2015Chen H, Gao S, Li J, Liu D, Sheng C, Yao C, Jiang W, Wu J, Chen S, Huang W.and Myc-His-EED were translated in vitro with TNT T7 coupled reticulocyte lysate system (Promega, #L4611) according to the manufacturer’s instructions. The in vitro translated products were used to perform

(12) LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3BMC biologyMarch 27, 2014Li Y, Pan J, Wei C, Chen J, Liu Y, Liu J, Zhang X, Evans SM, Cui Y, Cui S.in vitro transcription and translation of Isl1 using the TNT Coupled Reticulocyte Lysate System (Promega; L4611) and pcDNA3.1 was used as control. 5′-biotin-labeled oligonucleotides were obtained from Sangon Biotech

(13) New roles for Nanos in neural cell fate determination revealed by studies in a cnidarianDevelopmentAugust 15, 2013Justyna Kanska, Uri Frankin vitro transcription-translation system TNTHT7 Coupled Reticulocyte Lysate System (Promega, cat.no. L4611). We added 1 ml of 1 mM Nanos2 morpholino to 50 ml transcription-translation reaction. As control, a transcription-translation

(14) Critical loss of CBP/p300 histone acetylase activity by caspase-6 during neurodegenerationThe EMBO JournalDecember 15, 2003Caroline Rouaux, Natasa Jokic, Corinne Mbebi, Stephanie Boutillier, Jean-Philippe Loeffler, Anne-Laurence Boutillier[35S]methionine-labeled CBP and HDM1 was performed using the TNTâ Coupled Reticulocyte Lysate Systems kit (L4611) purchased from Promega, according to the 6547 C.Rouaux et al. manufacturer's procedures. [35S]Methionine

(15) Slit2 Guides Both Precrossing and Postcrossing Callosal Axons at the MidlineIn VivoThe Journal of NeuroscienceSeptember 3, 2003Tianzhi Shu, Vasi Sundaresan, Margaret M. McCarthy, Linda J. Richardsconsistent with previous findings (Brose et al., 1999). In the control lane (A, lane 1), an sate system, l4611; Promega, Madison, WI) was empty vector was added to the reaction, and, thus, no protein was produced.

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