TNT SP6 High-Yield Wheat Germ Protein Expression System from Promega

Product Specs
ItemTNT SP6 High-Yield Wheat Germ Protein Expression System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL3261
Quantity10 reactions
Systemwheat germ extract

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Compound Inflorescence (S) represses fruit growth and seed development in tomatoMolecular HorticultureJanuary 8, 2026Lin Weng, Li Wang, Enbai Zhou, Guixiang Wang, Xiaoyan Wang, Rong Li, Yuan Sun, Meng Li, Han Xiaoprotein was synthesized using the TnT™ SP6 High-Yield Wheat Germ Protein Expression System (Cat #, L3261, Promega, USA) and incubated with biotin- or CY5- labeled probes for 20 min at room temperature. Biotin-labeled

(2) The specificity landscape of WRKY transcription factors reveals the bidirectional influence of non-CG methylationNucleic Acids ResearchNovember 20, 2025Nana Ma, Dingkun Jiang, Tian Li, Lin Luo, Hao Chen, Xinfeng Zhang, Zongkai Mu, Shuyan Chen, Piaojuan Chen, Qing Liu, et al.HALO-tagged WRKYs were expressed using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega, L3261) following the manufacturer’s instructions: ∼2 μg of plasmid DNA was added into a 50 μl reaction and

(3) An acentrosomal aster with atypical microtubule polarity recruits cytokinesis signals to its center in Xenopus egg extractsJournal of Cell ScienceSeptember 15, 2025Ling Jin, Muchen Liu, Xianrui ChengBio-Rad). GFP–MKLP1 was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3261, Promega), following the manufacturer's protocol for in vitro protein synthesis. After the in vitro

(4) Mechanistic origins of temperature scaling in the early embryonic cell cycleNat CommunAugust 28, 2025Jan Rombouts, Franco Tavella, Alexandra Vandervelde, Connie Phong, James E. Ferrell, Qiong Yang, Lendert Gelensin vitro translation using the TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega, Cat. No. L3261). For each reaction, 3–4 μL of translated securin-CFP was added to 50–80 μL of Xenopus egg extract.

(5) The human ciliopathy protein RSG1 links the CPLANE complex to transition zone architectureNat CommunJuly 1, 2025Neftalí Vazquez, Chanjae Lee, Irene Valenzuela, Thao P. Phan, Camille Derderian, Marcelo Chávez, Nancie A. Mooney, Janos Demeter, Mohammad Ovais Aziz-Zanjani, Ivon Cusco, et al.reading frames (ORFs) with the TNT SP6 Hig-Yield Wheat Germ Protein Expression System (Promega Cat# L3261). The in vitro translated GFP-tagged and Myc-tagged proteins were first incubated together in binding/wash

(6) Specificity landscapes of 40 R2R3‐MYBs reveal how paralogs target different cis‐elements by homodimeric bindingiMetaMarch 5, 2025Tian Li, Hao Chen, Nana Ma, Dingkun Jiang, Jiacheng Wu, Xinfeng Zhang, Hao Li, Jiaqing Su, Piaojuan Chen, Qing Liu, et al.Halo tag were expressed using the TnT® SP6 High‐Yield Wheat Germ Protein Expression System (Promega, L3261). For each AtMYB protein, 3 µg plasmids were used in a 50 µL reaction and incubated for 2 h at 25°C;

(7) A Spatiotemporal Transcriptome Reveals Stalk Development in Pearl MilletInternational Journal of Molecular SciencesSeptember 10, 2024Fei Mao, Lin Luo, Nana Ma, Qi Qu, Hao Chen, Chao Yi, Mengxue Cao, Ensi Shao, Hui Lin, Zhanxi Lin, et al.subsequently expressed using the TnT® SP6 High Yield Wheat Embryo Protein Expression System (Promega, L3261, Fitchburg, WI, USA). The expression system was prepared as follows: 3 µg of reconstituted plasmid was

(8) Single-molecule imaging reveals the mechanism of bidirectional replication initiation in metazoaCellJuly 25, 2024Riki Terui, Scott E. Berger, Larissa A. Sambel, Dan Song, Gheorghe Chistolreactions were performed using TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega, Cat# L3261) according to the manufacturer’s instructions. Proteins were not purified after their production by

(9) Viscosity-dependent control of protein synthesis and degradationNat CommunMarch 8, 2024Yuping Chen, Jo-Hsi Huang, Connie Phong, James E. FerrellSP6-securin-CFP plasmid in 20 µL H2O with 30 µL SP6 High-Yield Wheat Germ Protein Expression System (TnT® L3261, Promega), and incubating at room temperature for 2 h per the manufacturer’s instructions. CSF extracts

(10) Protein homeostasis from diffusion-dependent control of protein synthesis and degradationbioRxivMay 3, 2023Yuping Chen, Jo-Hsi Huang, Connie Phong, James E. FerrellSP6-securin-CFP plasmid in 20 μL H2O with 30 μL SP6 High-Yield Wheat Germ Protein Expression System (TnT® L3261, Promega), and incubating at room temperature for 2 h per the manufacturer’s instructions. CSF extracts

(11) Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to ciliaMolecular biology of the cellMarch 1, 2023Palicharla VR, Hwang SH, Somatilaka BN, Legué E, Shimada IS, Familiari NE, Tran VM, Woodruff JB, Liem KF Jr, Mukhopadhyay S.by in vitro translation using the TNT Sp6 high-yield wheat germ protein expression system (Promega; L3261) from pCS2-Myc-ARL13B vectors generated by gateway cloning. GFP-SNAP-ARL13B was generated by cloning

(12) DNA demethylation affects imprinted gene expression in maize endospermGenome BiologyDecember 1, 2022Qiang Xu, Leiming Wu, Zhixiang Luo, Mei Zhang, Jinsheng Lai, Lin Li, Nathan M. Springer, Qing Licontrol. The ZmO2 and GFP proteins were expressed in vitro using TNT SP6 Wheat Germ Master Mix (Promega L3261) following the manufacturer’s instruction. Separately, DNA from WT and zmros1ab mutant endosperm was

(13) Variation in the fruit development gene POINTED TIP regulates protuberance of tomato fruit tipNature CommunicationsOctober 8, 2022Jianwen Song, Lele Shang, Changxing Li, Wenqian Wang, Xin Wang, Chunli Zhang, Guo Ai, Jie Ye, Changxian Yang, Hanxia Li, et al.PTR-pF3K and PTH-pF3K plasmids were added to the TnT® SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega, USA) and incubated at 25 °C for 2 h to express the proteins in vitro. EMSAs were performed

(14) Global Profiling of 2-hydroxyisobutyrylome in Common WheatGenomics, proteomics & bioinformaticsAugust 1, 2022Zhang N, Zhang L, Li L, Geng J, Zhao L, Ren Y, Dong Z, Chen F.Halo-His-gene was expressed using a 30 μl TNT SP6 High-Yield Wheat Germ Protein Expression System (Catalog No. L3261, Promega). Expression levels were determined by SDS-PAGE and WB. Magne Halo Tag Beads (Catalog No. G728A,

(15) Regulator of G protein signaling 2 inhibits Gα(q)-dependent uveal melanoma cell growthThe Journal of biological chemistryJune 1, 2022Zhang Q, Haak AJ, Sjögren B.In vitro transcription/translation and co-IP TnT SP6 High-Yield Wheat Germ Protein Expression System kit (L3260; Promega) was used as directed by the manufacturer. The transcription/translation reaction was performed

(16) The Emerging Role of E3 Ubiquitin Ligase SMURF2 in the Regulation of Transcriptional Co-Repressor KAP1 in Untransformed and Cancer Cells and TissuesCancersMarch 22, 2022Shah PA, Boutros-Suleiman S, Emanuelli A, Paolini B, Levy-Cohen G, Blank M.

(17) CEP97 phosphorylation by Dyrk1a is critical for centriole separation during multiciliogenesisThe Journal of cell biologyJanuary 3, 2022Lee M, Nagashima K, Yoon J, Sun J, Wang Z, Carpenter C, Lee HK, Hwang YS, Westlake CJ, Daar IO.and Dvl2 proteins were prepared using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260; Promega) according to the manufacturer’s instructions for the CEP97 protein shift assay. For in vitro

(18) Bistable, Biphasic Regulation of PP2A-B55 Accounts for the Dynamics of Mitotic Substrate PhosphorylationCurrent biology : CBFebruary 22, 2021Kamenz J, Gelens L, Ferrell JE Jr.was in vitro translated using the TNT SP6 high-yield wheat germ protein expression system (Promega, #L3261). Three or 4 μL of in vitro translated securin-CFP was added to 50 or 80 μL extract. The extract was

(19) Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed GerminationInternational Journal of Molecular SciencesFebruary 7, 2021Cheng-Chao Zhu, Chu-Xin Wang, Chen-Ya Lu, Jin-Dong Wang, Yu Zhou, Min Xiong, Chang-Quan Zhang, Qiao-Quan Liu, Qian-Feng Li#G184A). OsbZIP09 fused to HaloTag was expressed using a TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega, Madison, WI, USA), and was purified using Magne HaloTag Beads (G7281, Promega). The Magne

(20) Biofoundry-assisted expression and characterization of plant proteinsSynthetic biology (Oxford, England)January 1, 2021Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron NJ.germ cell-free expressions used the TNT® SP6 High-Yield Wheat Germ Protein Expression System L3260/L3261 (Promega) according to manufacturers’ instructions. Plasmids were added at a final concentration of

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