UNICORN™ 7 control software from Cytiva

Product Specs
ItemUNICORN™ 7 control software
CompanyCytiva Life Sciences
Price Get Pricing Get Quote for this Product
Catalog NumberUNICORN 7
QuantityEA
Electrical Requirements300 VA

Cytiva Life Sciences

(1) Immunogenicity and protective efficacy of MERS CoV, NL140422, and HKU4 spike protein nanoparticle vaccinesnpj VirusesFebruary 19, 2026Peter J. Halfmann, Jeong Soo Lee, Tong Wang, Augustine Duffy, Yoshihiro Kawaoka, Ravi S. Kanecontained 8 μg of S. The solution was run on Superdex 200 Increase 10/300 Column (Cytiva) using the Unicorn 7 control system (Cytiva) and was eluted with 1 CV of PBS at 0.65 mL/minute. The absorbance at 210 nm

(2) On-Column Dual-Gradient Refolding for Efficient Recovery of Insoluble Affinity-Tagged Recombinant ProteinsBIO-PROTOCOLFebruary 5, 2026Anna Vlaskina, Dmitry Petrenko, Yulia Agapova, Anastasia Kuzminkova, Marta Evteeva, Maxim Patrushev223TV) 24. Refrigerator (2–8 °C) ХЛ-250 POZIS (Pozis, catalog number: 223TV) Software and datasets 1. UNICORN 7 control software (Cytiva) 2. Image Lab Software (Bio-Rad) Procedure A. Expression of recombinant proteins Note: In

(3) Mechanisms of HSV-1 helicase-primase inhibition and replication fork complex assemblyCellJanuary 22, 2026Zishuo Yu, Pradeep Sathyanarayana, Cong Liu, Joel M.J. Tan, Pan Yang, Biswajit Das, Side Hu, Xiaoyi Fan, Chenggong Ji, Sandra K. Weller, et al.RRID:SCR_002798" UNICORNTM version 7.8,Cytiva,https://www.cytivalifesciences.com/en/us/shop/chromatography/software/unicorn-7-p-05649 AlphaFold Server,"Abramson et al., 202441","https://deepmind.google/technologies/alphafold/alphafold-server/,

(4) Targeted computational design of an interleukin-7 superkine with enhanced folding efficiency and immunotherapeutic efficacyeLifeJanuary 16, 2026See-Khai Lim, Wen-Ching Lin, Yi-Chung Pan, Sin-Wei Huang, Yao-An Yu, Cheng-Hung Chang, Che-Ming Jack Hu, Chung-Yuan Mou, Kurt Yun Mouand (D) Neo-7-Q6P-T45I. Percentage of purity is calculated from the SEC-FPLC peak profile via Cytiva Unicorn 7 software after affinity chromatography purification. SPR (Biacore) characterization of the binding kinetics

(5) De novo design of protein nanoparticles with integrated functional motifsbioRxivJanuary 5, 2026Cyrus M. Haas, Sanela Rankovic, Hanul K. Lewis, Kenneth D. Carr, Connor Weidle, Sophie S. Gerdes, Lily R. Nuss, Felicitas Ruiz, Syed Moiz, Maggie Fiorelli, et al.500 mM NaCl, 100 mM L-Arginine). Instrument control and elution profile analysis were performed using UNICORN 7 software (Cytiva). HEK293F expression and purification Plasmids for mammalian expression were purified

(6) A novel chromatography method for high purity production of Recombinant human parathyroid hormone from E. coliScientific ReportsDecember 30, 2025Farhad Mollasoltani, Milad Adak, HomaSadat Esfahani, Elaheh Roshani Yasaghi, Sirwan Khanchezarwith Unicorn 7.0 version software (https://www.cytivalifesciences.com/en/us/shop/cell-therapy/software/unicorn-7-p-05649?psmenu=0, Cytiva, Uppsala, Sweden). Capto™ Adhere Impres resin (Catalog Number: [17371502],

(7) Molecular characterisation of the Bacillus subtilis SpbK antiphage defence systemNature CommunicationsDecember 29, 2025Biswa P. Mishra, Christian L. Loyo, Yanyao Cai, Thomas Litfin, Gause Miraj, Lou Brillault, Veronika Masic, Tamim Mosaiab, Premraj Rajaratnam, Santosh Rudrawar, et al.Pure device (Cytiva) at 0.5 mL/min. Analysis of chromatograms and image preparation were performed in Unicorn 7 software (Cytiva) and Excel (Microsoft). Peak fractions were analysed by SDS-PAGE. Mass photometry Mass

(8) From sequence to scaffold: Computational design of protein nanoparticle vaccines from AlphaFold2-predicted building blocksProceedings of the National Academy of SciencesNovember 11, 2025Cyrus M. Haas, Naveen Jasti, Annie Dosey, Joel D. Allen, Rebecca Gillespie, Jackson McGowan, Elizabeth M. Leaf, Max Crispin, Cole A. DeForest, Masaru Kanekiyo, et al.25 mM Tris pH 8.0, 150 mM NaCl. Instrument control and elution profiles analysis were performed with UNICORN 7 software (Cytiva). Mammalian Protein Expression and Purification. For purification of plasmid DNA

(9) Structural basis of apoptosis induction by the mitochondrial voltage-dependent anion channelNat CommunOctober 27, 2025Melina Daniilidis, Umut Günsel, Georgios Broutzakis, Kira D. Leitl, Robert Janowski, Kai Fredriksson, Dierk Niessing, Christos Gatsogiannis, Franz HagnNi-NTA and a HiLoad 16/600 Superdex 200 pg column (Cytiva) on an Äkta Purifier system controlled by Unicorn 7 software. For the preparation of nanodiscs for cryo-EM, the size-exclusion chromatography buffer was

(10) Production of Homogeneous, Functional Zinc-Finger Arrays in High Yield With Two Chromatographic StepsBio ProtocAugust 20, 2025Jingchang Liang, Maia Azubel, Guanqiao Wang, Yan Nie, Roger D. Kornberg, Andrew J. Beel, Pierre-Jean MatteïÄKTA purifier (GE Healthcare, https://www.cytivalifesciences.com/en/us/shop/chromatography/software/unicorn-7) Procedure A. Transformation of E. coli BL21 (DE3) with ZF-array expression plasmids 1. DNA fragments

(11) De Novo Design of High-Performance Cortisol Luminescent BiosensorsJournal of the American Chemical SocietyAugust 6, 2025Julie Yi-Hsuan Chen, Xue Peng, Chenggang Xi, Gyu Rie Lee, David Baker, Andy Hsien-Wei Yehwithout further purification. Size exclusion chromatography was performed using a KTA pure M system with UNICORN 7 Workstation control (GE Healthcare) coupled with a Superdex 75 Increase 10/300 GL column. DNA and protein

(12) DNA-encoded library screening uncovers potent DNMT2 inhibitors targeting a cryptic allosteric binding siteiScienceAugust 5, 2025Ariane F. Frey, Merlin Schwan, Annabelle C. Weldert, Valerie Kadenbach, Jürgen Kopp, Zarina Nidoieva, Robert A. Zimmermann, Lukas Gleue, Collin Zimmer, Marko Jörg, et al.compound 3. The resulting chromatograms were analyzed by superposing their 280 nm signals using the Unicorn7 evaluation software (Cytiva). Crystallization, data collection, model building, and refinement Crystals

(13) Teicoplanin Nonribosomal Peptide Synthetase Is Unable to Incorporate Alpha-Ketoacid Building BlocksBiochemistryMay 6, 2025Minuri S. Ratnayake, Xinyun Jian, Julien Tailhades, Gregory L. Challis, Mathias H. Hansen, Józef R. Lewandowski, Max J. Crylecrude, 5 mL, Cytiva) pre-equilibrated in lysis buffer using an Akta Start Purification System (running Unicorn 7, Cytiva). The target protein was eluted in fractions with a linear gradient from 10 mM to 300 mM imidazole

(14) Antibody Purification Using Spy ChemistryBio ProtocApril 20, 2025Xiaofeng Yang, Zhanglin Lin, Ya Xiang, Zisha LaoAKTA purifier (GE Healthcare, https://www.cytivalifesciences.com/en/us/shop/chromatography/software/unicorn-7-p-05649 ) Procedure A. Protein expression 1. Transform E. coli BL21(DE3) bacterial strain with the

(15) A Robust and Easy Protein Purification Method Using SpyDock-Modified ResinBIO-PROTOCOLApril 20, 2025Xiaofeng Yang, Zhanglin Lin, Ya Xiang, Binrui Chen, Zisha LaoAKTA purifier (GE Healthcare, https://www.cytivalifesciences.com/en/us/shop/chromatography/software/unicorn-7-p-05649) Procedure Note: Perform most procedures at room temperature (25 °C), except for steps where

(16) PglZ from Type I BREX phage defence systems is a metal-dependent nuclease that forms a sub-complex with BrxBbioRxivMarch 29, 2025Jennifer J. Readshaw, Lindsey A. Doyle, Maria Puiu, Abigail Kelly, Andrew Nelson, Alex J. Kaiser, Sydney McGuire, Julieta Peralta-Acosta, Darren L. Smith, Barry L. Stoddard, et al.volume directly proportional to Vo. Elution volumes (Ve) were calculated using the Peaks function in Unicorn™ 7 (Cytiva) and converted to partitioning coefficients (Kav) using the following equation: Kav=Ve−VoVc−Vo Molecular

(17) De novo luciferases enable multiplexed bioluminescence imagingChemMarch 13, 2025Julie Yi-Hsuan Yi-Hsuan Chen, Qing Shi, Xue Peng, Jean de Dieu de Dieu Habimana, James Wang, William Sobolewski, Andy Hsien-Wei Hsien-Wei Yehwere purchased from Fisher Scientific or VWR and used without further purification. ÄKTA pure M with UNICORN 7 Workstation control (Cytiva) coupled with a Superdex 75 Increase 10/300 GL or Superdex 200 Increase 10/300 GL

(18) TCR catch bonds nonlinearly control CD8 cooperation to shape T cell specificityCell ResearchFebruary 27, 2025Rui Qin, Yong Zhang, Jiawei Shi, Peng Wu, Chenyi An, Zhenhai Li, Nuo Liu, Ziyan Wan, Ting Hua, Xiaolong Li, et al.buffer exchange. All purifications were performed on ӒKTA goTM chromatography system and analyzed via UNICORN 7. Purification of biotinylated pMHC proteins The procedures of pMHC-I protein purification have been

(19) A method for facile production of variable lymphocyte receptors using SHuffle Escherichia coliBiotechnology ProgressJanuary 23, 2025Elizabeth A. Appelt, James B. Thoden, Candis Dancy, Hannah D. Bachmeier, Seth A. Gehrke, Melanie A. Staffenson, Ivan Rayment, Moriah E. Katt, Hazel M. Holden, Eric V. Shustaphase. 0.2 mL samples were injected and eluted with a flowrate of 0.75 mL/min. Spectra were analyzed in Unicorn 7. 2.9 ECM binding assay and K D calculation of P1C10 bEnd.3 (ATCC #CRL2299) culture was stored in

(20) Multivalent S2 subunit vaccines provide broad protection against Clade 1 sarbecoviruses in female miceNat CommunJanuary 7, 2025Peter J. Halfmann, Raj S. Patel, Kathryn Loeffler, Atsuhiro Yasuhara, Lee-Ann Van De Velde, Jie E. Yang, Jordan Chervin, Chloe Troxell, Min Huang, Naiying Zheng, et al.PBS. They were loaded into a Superdex 200 Increase 10/300 Column (Cytiva) using an ÄKTA Pure and the Unicorn 7 control system (Cytiva). They were eluted with a full column volume of PBS flowing at 0.65 mL/min, while

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