Anti-Nucleus MicroBeads from Miltenyi Biotec

(1) Patient-derived tau-seeded human neuronal chimeras recapitulate mature Alzheimer's tau pathology and uncover human-specific neuronal vulnerabilitybioRxivJuly 9, 2026Yanru Ji, Purba Mandal, Jiahui Liu, Wen Hu, Bryce David Colón, Changlin Wan, Raymond W Pan, Dongkai Guo, Lijia Zhang, Xingjian Gu, et al.with minimum cell debris, the suspension was treated with Anti-Nucleus Microbeads (Miltenyi Biotec, 130–132-997). After purification, nuclei were washed with PBS and proceed to library preparation using Chromium

(2) Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencingSTAR ProtocJuly 8, 2026Lei Li, Jonathan G. Pol, Christophe Desterke, Wanchao Hu, Nicolas Trainel, Morgane Benardeau, Manuela Lizarralde-Guerrero, Maria Chiara Maiuri, Guido Kroemer, Gabriel Perlemuter, et al."Liver Dissociation Kit, mouse",MILTENYI BIOTEC,Cat#130-105-807 Anti-Nucleus MicroBeads,MILTENYI BIOTEC,Cat#130-132-997 BD Rhapsody™ cDNA Kit,BD Biosciences,Cat#633773 BD Rhapsody™ Enhanced Cartridge Reagent Kit,BD Biosciences,Cat#667052 BD Rhapsody

(3) Targeting FGFR signaling overcomes therapeutic resistance and immune evasion in oncogenic PIK3CA-driven serous-like endometrial cancerNature CommunicationsMay 4, 2026Xin Cheng, Yadong Zhang, Changli Qian, Erica Holdridge, Guruprasad Ananda, Tao Jiang, Jing Ni, Shaozhen Xie, Hao Gu, Renlei Ji, et al.minutes at 4ْ°C. Nuclei pellets were further purified using Anti-Nucleus MicroBeads (Miltenyi Biotec, #130-132-997) following the manufacturer’s instructions. Purified nuclei were resuspended in 100 μL resuspension

(4) Mapping glioblastoma’s isoform diversity using long-read single-cell analysisNature CommunicationsApril 23, 2026Wenshu Tang, Cario W. S. Lo, Annie T. W. Chu, Wing Lun Lee, Dingyuan Wang, Karrie Mei-Yee Kiang, Lai-Fung Li, Gilberto K. K. Leung, Hong Kong Genome Project, Aya El Helali, et al.min at 4 °C. The resulting pellet was resuspended in Nuclei Resuspension Buffer and incubated with Anti-Nucleus MicroBeads (Miltenyi Biotec) for 15 min at 4 °C. Labeled nuclei were isolated using magnetic separation with LS columns

(5) Frontal Brain Injury Reduces Sensitivity to Reward-Predictive Cues and Remodels the Nucleus AccumbensbioRxivApril 19, 2026Erskine Chu, Jenna E. McCloskey, Mia A. Eleid, Sathvik Jami, Alexandra G. Dorinsky, Fikir B. Arega, Kris M. Martens, Fangli Zhao, Jonathan M. Packer, Patrick Stevens, et al.and the Acridine Orange/Propidium Iodide Stain (Logos Biosystems) as per manufacturer’s instructions. Anti-nucleus microbeads (Miltenyi) was applied to nuclei suspension as per manufacturer’s instructions. Nuclei were then magnetically

(6) Autoimmune neuroinflammation leads to neuronal death via MIF nuclease-mediated parthanatosNature NeuroscienceFebruary 17, 2026Jackson W. Mace, Sachin P. Gadani, Matthew D. Smith, Danny Galleguillos, Bong Gu Kang, Milton Roy, Meilian Liu, Benjamin Summers, Thomas Garton, Marjan Gharagozloo, et al.that had greater than 2 × 106 nuclei with additional NSB. Anti-nucleus microbeads (Miltenyi, cat. no. 130-132-997) were then added at a ratio of 50 μl beads per 1 × 106 nuclei and pipette-mixed then incubated at 4

(7) Single-nuclei RNA-sequencing uncovers sexually divergent exercise signatures partially mimicked by TFEB overexpression in mouse skeletal musclebioRxivJanuary 30, 2026Katrina Granger, Kailin Liu, Tiarra Joseph, Ari Adler, Ian Matthews, Jocelyne Leon, Clayton Baker, Minhoo Kim, Hu Wang, Xianlin Han, et al.Nuclei Extraction Buffer (Cat # 130–128-024). Lysate was purified using Miltenyi Anti-Nucleus Microbeads (Cat #130–132-997) and LS Columns (Cat # 130–042-401). Nuclei were counted using MACSQuant Analyzer 10 Flow Cytometer

(8) Nuclei Isolation Methods on Frozen Clotted Blood SamplesBIO-PROTOCOLJanuary 20, 2026Melissa Cuevas, Kenneth Jones, Nancy Hadley Miller(Invitrogen, catalog number: 01-2222-41) 4. Anti-nucleus microbeads (Miltenyi Biotec, catalog number: 130-132-997) 5. RNase inhibitor (Applied Biosystems, catalog number: N8080119) 6. Ethanol 200 proof (Decon, catalog

(9) Nuclei Isolation Methods on Frozen Clotted Blood SamplesBio ProtocJanuary 20, 2026Melissa Cuevas, Kenneth Jones, Nancy Hadley Miller(Invitrogen, catalog number: 01-2222-41) 4. Anti-nucleus microbeads (Miltenyi Biotec, catalog number: 130-132-997) 5. RNase inhibitor (Applied Biosystems, catalog number: N8080119) 6. Ethanol 200 proof (Decon, catalog

(10) CRISPR screening reveals genetic regulators associated with the evolution of eye degenerationbioRxivJanuary 3, 2026Devin Shennard, Charlotte Mulliniks, Estephany Ferrufino, Aubrey Manning, Stefan Choy, Rianna Ambosie, Shiyanth Thevasagayampillai, Brooke Pileggi, Lane Zimmermann, Chenxiao Shen, et al.SmartStrainer to ensure a homogeneous single-nucleus suspension. Nuclei were magnetically labelled with Anti-Nucleus MicroBeads (Miltenyi Biotec) for enrichment and debris removal. One million nuclei were resuspended in 450 μL of

(11) A Single-Cell Omics Technical Guide for Advancing Neuropsychiatric ResearchGenes (Basel)November 21, 2025Kayleigh Casmey, Maria Zimmermann, Yuxin Xie, Sierra A. Codeluppi-Arrowsmith, Gustavo TureckiMO, USA), 10x Genomics Chromium Nuclei Isolation Kit (10x Genomics, Pleasanton, CA, USA), Miltenyi Anti-Nucleus MicroBeads (Bergisch Gladbach, Germany), Miltenyi Debris Removal Solution (Bergisch Gladbach, Germany), and Miltenyi

(12) Plexin-B1 safeguards astrocyte agility and glial alignment to facilitate wound corralling and axon pathfinding in mouse spinal cord injury modelNature communicationsNovember 18, 2025Ni H, Zhou Z, Estill M, Halawani D, Junqueira Alves C, Shen L, Xie N, Friedel RH, Zou H.#130-128-024). The resulting suspension was enriched for nuclei using anti-Nucleus MicroBeads (Miltenyi Biotec, #130-132-997). Nuclei were subsequently fixed using the Evercode Nuclei Fixation Kit v3 (Parse Biosciences, ECFN3300). Split-seq

(13) A simple approach of nuclei isolation for single nucleus multiome sequencingJournal of Molecular and Cellular CardiologyNovember 1, 2025Yin Wang, Di Ren, Randy Kang, Kai Zhang, Yunqian Peng, Heather Zhou, Geming Lu, Junjie Guo, Adolfo Garcia-Ocaña, Yingfeng Deng, et al.homogenization, nuclei suspension was incubated with anti-nucleus micro-beads (Miltenyi Biotec., cat #130-132-997). After mixing with separation buffer, nuclei suspension was proceeded to magnetic separation by a MACS

(14) Capicua Refines Mossy Fiber–CA3 Axon Targeting in the Late Postnatal HippocampusThe FASEB JournalSeptember 30, 2025Rebekah van Bruggen, Karla Manzanet Freyre, Mi Wang, Qiumin Tan1000493) lysis buffer, and the debris was removed using Miltenyi Anti‐Nucleus beads (Miltenyi Biotec, Cat# 130‐132‐997). Briefly, frozen tissue pieces were pooled, weighed, and chopped into small pieces on dry ice before

(15) Capicua Refines Mossy Fiber–CA3 Axon Targeting in the Late Postnatal HippocampusFASEB JSeptember 22, 2025Rebekah van Bruggen, Karla Manzanet Freyre, Mi Wang, Qiumin Tan1000493) lysis buffer, and the debris was removed using Miltenyi Anti‐Nucleus beads (Miltenyi Biotec, Cat# 130‐132‐997). Briefly, frozen tissue pieces were pooled, weighed, and chopped into small pieces on dry ice before

(16) Diverse cell types establish a pathogenic immune environment in peripheral neuropathyJ NeuroinflammationMay 23, 2025Julie Choi, Amy Strickland, Hui Qi Loo, Wendy Dong, Lilianne Barbar, A. Joseph Bloom, Yo Sasaki, Sheng Chih Jin, Aaron DiAntonio, Jeffrey Milbrandtcold wash buffer. The samples were further purified using Anti-Nucleus MicroBeads (Miltenyi Biotec 130-132-997) per manufacturer’s manuals. The purified single nuclei were used to prepare snRNA-seq libraries following

(17) Single cell multiomics reveals drivers of metabolic dysfunction-associated steatohepatitismedRxivMay 11, 2025Weston Elison, Lei Chang, Yang Xie, Charlene Miciano, Qian Yang, Hannah Mummey, Ryan Lancione, Sierra Corban, Sadatsugu Sakane, Jacinta Lucero, et al.then filtered through 100-μm and 30-μm CellTrics™ Filters and centrifuged for 5 min at 500g at 4 C. Anti-Nucleus MicroBeads (Miltenyi Biotec) were used for debris removal and enrichment of nuclei. The nuclei suspension was centrifuged

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Anti-Nucleus MicroBeads

Anti-Nucleus MicroBeads from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Anti-Nucleus MicroBeads from Miltenyi Biotec

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