Chromatography Columns from Bio-Rad

Product Specs
ItemChromatography Columns
CompanyBio-Rad
Price Supplier Page View Company Product Page
QuantityEA
ModeIon Exchange, Mixed Mode, Affinity, Size Exclusion / Desalting

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

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Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) NSUN2-mediated M(5)c methylation of IRF3 mRNA negatively regulates type I interferon responses during various viral infectionsEmerging microbes & infectionsDecember 1, 2023Wang H, Feng J, Zeng C, Liu J, Fu Z, Wang D, Wang Y, Zhang L, Li J, Jiang A, et al.60°C for 1 h, and this process was repeated for 4 cycles, followed by desalting using Micro Bio-spin 6 Chromatography Columns (Biorad, 732-6200). Then, the RNA adducts were desulfonated by adding 1 volume of Tris–HCl (pH 9.0)

(2) NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systemsNature CommunicationsJune 6, 2023Pamela E. Molinari, Adriana R. Krapp, Andrea Weiner, Hannes M. Beyer, Arun Kumar Kondadi, Tim Blomeier, Melina López, Pilar Bustos-Sanmamed, Evelyn Tevere, Wilfried Weber, et al.reduced by 10 mM DTT for 6 min on ice, and the fully reduced protein was desalted with Micro Bio-Sin® 6 Chromatography Columns (BioRad). Oxidation was carried out with 0.5-1 mM NADP+, 10 µM thioredoxin-A from Escherichia coli or

(3) An association of CSF apolipoprotein E glycosylation and amyloid-beta 42 in individuals who carry the APOE4 alleleAlzheimer's Research & TherapyMay 23, 2023Cristiana J. Meuret, Yueming Hu, Sabrina Smadi, Mikaila Ann Bantugan, Haotian Xian, Ashley E. Martinez, Ronald M. Krauss, Qiu-Lan Ma, Dobrin Nedelkov, Hussein N. Yassinerotator. The samples were then equilibrated to room temperature and transferred to Bio-spin Disposable Chromatography Columns (BIO-RAD, Cat. No. 732–6008). The columns were first washed with 10 bed volumes of 1 \documentclass[12pt]{minimal}

(4) The flavagline FL3 interferes with the association of Annexin A2 with the eIF4F initiation complex and transiently stimulates the translation of annexin A2 mRNAFrontiers in Cell and Developmental BiologyMay 12, 2023Ann Kari Grindheim, Sudarshan S. Patil, Canan G. Nebigil, Laurent Désaubry, Anni Vedeleraliquoting 2 identical samples (= 60 µL slush) for each experimental condition into small microBio-Spin Chromatography Columns (#7326204EDU; Bio-Rad laboratories, Hercules, United States). 27 μL of 1 M imidazole (final concentration

(5) Nanoparticle‐mediated genome editing in single‐cell embryos via peptide nucleic acidsBioengineering & Translational MedicineMay 1, 2023Rachael Putman, Adele S. Ricciardi, Kelly E. W. Carufe, Elias Quijano, Raman Bahal, Peter M. Glazer, W. Mark Saltzmanfor 6 h then heat inactivated at 65°C for 10 min. Samples were purified using MicroBio‐Spin P‐30 Tris Chromatography columns (BioRad, Hercules, CA). Columns were inverted to resuspend the gel matrix, then centrifuged at 1000

(6) Snake venom-defined fibrin architecture dictates fibroblast survival and differentiationNature communicationsFebruary 23, 2023Wang Z, Lauko J, Kijas AW, Gilbert EP, Turunen P, Yegappan R, Zou D, Mata J, Rowan AE.purchased from ThermoFisher Scientific. The 15-well chamber slides (#81506) were purchased from Ibidi, USA. Chromatography columns (Poly-Prep, #731-1550) were bought from Bio-Rad. Cell culture inserts (3 μm filter, #MCSP24H48) and

(7) Fine Particle Adsorption Capacity of Volcanic Soil from Southern Kyushu, JapanNanomaterials (Basel)January 30, 2023Naoaki Misawa, Kentaro Yasui, Kentaro Sakai, Taichi Kobayashi, Hideki Nagahama, Tomohiro Haraguchi, Satomi Sasaki, Vetchapitak Torrung, Taradon Luangtongkum, Takako Taniguchi, et al.heat at 453 K for 30 min. A column with a diameter of 15 mm and height of 100 mm (Econo-Pac Disposable Chromatography Columns, BioRad, Tokyo, Japan) was filled with 5 g of a soil sample. Then, 10 mL of an E. coli (strain ATCC

(8) Characterization of a glycan-binding complex of minor pilins completes the analysis of Streptococcus sanguinis type 4 pili subunitsProceedings of the National Academy of SciencesJanuary 17, 2023Meriam Shahin, Devon Sheppard, Claire Raynaud, Jamie-Lee Berry, Ishwori Gurung, Lisete M. Silva, Ten Feizi, Yan Liu, Vladimir Pelicicsteps: affinity chromatography and SEC. 6His-tagged proteins were affinity-purified using Gravity Flow Chromatography columns (Bio-Rad) loaded with Ni-NTA agarose resin (Qiagen). Strep-tagged proteins were affinity-purified on

(9) Strategic optimization of conditions for the solubilization of GST-tagged amphipathic helix-containing ciliary proteins overexpressed as inclusion bodies in E. coliMicrobial Cell FactoriesDecember 12, 2022Amruta A. Shendge, Jacinta S. D’Souzabinding, the beads along with the supernatant were added to the rinsed column (Econo-Pac® Disposable Chromatography Columns, Bio-Rad Catalogue-732-1010) and allowed to settle down for 2 min. The flow-through containing the unbound

(10) Preclinical studies for improving radiosensitivity of non-small cell lung cancer cell lines by combining glutaminase inhibition and senolysisTranslational OncologyJuly 1, 2022Masaki Fujimoto, Ritsuko Higashiyama, Hironobu Yasui, Koya Yamashita, Osamu Inanamiby sonication, and the obtained cell lysate was applied to an anion exchange resin (AG 1-X8 Polyprep Chromatography Columns, Bio-Rad Laboratories, Hercules, CA, USA) to isolate 3H-labeled glutamate synthesized by cellular GLS

(11) An increase in surface hydrophobicity mediates chaperone activity in N-chlorinated RidARedox BiologyJuly 1, 2022Marharyta Varatnitskaya, Julia Fasel, Alexandra MÃ¼ller, Natalie Lupilov, Yunlong Shi, Kristin Fuchs, Marco Krewing, Christoph Jung, Timo Jacob, Barbara Sitek, et al.the residual oxidants was achieved by size-exclusion chromatography using “Micro Bio-Spin P30 Tris Chromatography Columns” (Bio-Rad, München, Germany) or Nap-5 columns (GE Healthcare Life Sciences, Amersham, UK) according

(12) Oligomerization and Spatial Distribution of KvÎ²1.1 and KvÎ²2.1 Regulatory SubunitsFrontiers in PhysiologyJune 17, 2022Sara R. Roig, Silvia Cassinelli, Andre Zeug, Evgeni Ponimaskin, Antonio Felipefor 1 h at 4°C. Next, each sample was incubated in a small chromatography column (Bio–Rad Microspin Chromatography Columns), which contained 2.5 μg of anti-GFP antibody (Genescript) previously crosslinked to protein A Sepharose

(13) An effective strategy for ligand-mediated pulldown of the HER2/HER3/NRG1β heterocomplex and cryo-EM structure determination at low sample concentrationsMethods in EnzymologyMay 7, 2022Raphael Trenker, Devan Diwanji, Kliment A. Verba, Natalia Jura50 ml, polypropylene (VWR, #21008) Microcentrifuge tubes, 1.7 ml (VWR, #87003-294) Econo-Pac(R) Chromatography Columns, 20 ml bed volume (Bio-Rad, #7321011) Syringes and Millex-HV Syringe Filter Unit, 0.45 um, Millipore Sigma

(14) S-acylation-dependent membrane microdomain localization of the regulatory Kvβ2.1 subunitCellular and molecular life sciences : CMLSApril 9, 2022Roig SR, Cassinelli S, Navarro-Pérez M, Pérez-Verdaguer M, Estadella I, Capera J, Felipe A.Health care) for 1 h at 4 °C. Next, samples were incubated in a chromatography column (BioRad Micro spin Chromatography Columns), which contained 2.5 μg of anti-GFP antibody (Genescript) previously crosslinked to protein A Sepharose

(15) A Comparison of Antibacterial Properties of Tachyplesin, Thanatin, and Enterocin P on Enterococcus faecalisEuropean Endodontic JournalMarch 30, 2022Armita Rouhani, Afshin Javadzadeh, Abbas Tanhaeian, Sara Navabithis phase, dry resin (His-Select Nickel Affinity Gel, Sigma; 1 mL) was washed on an Econo-Pac column (Chromatography Columns; BIO-RAD) with 5 vol of water and 10 vol of buffer A (50mM NaH2PO4, 300 mM NaCl, 10 mM Imidazole (Sigma®);

(16) Generation and Characterization of an Inducible Cx43 Overexpression System in Mouse Embryonic Stem CellsCellsFebruary 16, 2022Niemann P, Schiffer M, Malan D, Grünberg S, Roell W, Geisen C, Fleischmann BK.(Agilent Technologies, Santa Clara, CA, USA). Labelled probes were purified with Bio-Spin P30 Tris Chromatography Columns (Bio-Rad) added to the prehybridized membrane, and hybridization was performed under stringent conditions

(17) GCN5 maintains muscle integrity by acetylating YY1 to promote dystrophin expressionJournal of Cell BiologyFebruary 7, 2022Gregory C. Addicks et al.New England Biolabs) according to the manufacturer’s protocol and purified using Micro Bio-Spin P-30 Tris Chromatography Columns (7326250; Bio-Rad Laboratories). YY1 was bound to oligonucleotide dimers in a solution of 10 mM Tris,

(18) MrTPS3 and MrTPS20 Are Responsible for β-Caryophyllene and α-Pinene Production, Respectively, in Red Bayberry (Morella rubra)Frontiers in Plant ScienceJanuary 7, 2022Yan Wang et al.purified using Ni-NTA Sefinose Resin (Settled Resin) (Sangon Biotech, China) and Econo-Pac® Disposable Chromatography Columns (Bio-Rad) as previously described (Yang et al., 2020). The purified His-MrTPS3 protein was dialyzed

(19) The Mitochondrial Routing of the Kv1.3 ChannelFrontiers in oncologyJanuary 1, 2022Capera J, Navarro-Pérez M, Moen AS, Szabó I, Felipe A.gentle mixing. Next, each sample was incubated in a small chromatography column (Bio-Rad MicroBio-Spin Chromatography Columns) containing 2.5 mg of anti-GFP antibody (GeneScript) that had been previously cross-linked to Protein

(20) Adipose Lipolysis Regulates Cardiac Glucose Uptake and Function in Mice under Cold StressInternational journal of molecular sciencesDecember 12, 2021Choi Y, Shin H, Tang Z, Yeh Y, Ma Y, Kadegowda AKG, Wang H, Jiang L, Arya RK, Chen L, et al.homogenized and deoxyglucose phosphate was separated from deoxyglucose using an ion-exchange column (Chromatography Columns #731-6211, Bio Rad) and then counted as described [73]. 4.6. Gene Expression Analysis Total RNA was

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Chromatography Columns from Bio-Rad

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Chromatography Columns from Bio-Rad

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