KAPA Pure Beads from Roche

Product Specs
ItemKAPA Pure Beads
CompanyRoche
Price Supplier Page View Company Product Page
Catalog Number07983271001
Quantity5 mL

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) Hybrid-seq deciphers the complex transcriptional profile of the human BRCA1 DNA repair associated geneRNA BiologyDecember 31, 2023Panagiotis G. Adamopoulos, Konstantina Athanasopoulou, Michaela A. Boti, Georgios Dimitroulis, Glykeria N. Daneva, Panagiotis Tsiakanikas, Andreas Scorilasselection (target fragment ~ 400bp) was followed to enrich the library for the desired fragments, using the KAPA Pure Beads (Kapa Biosystems Inc.) in the recommended dsDNA: beads ratio. In the next step, the quantification of

(2) A high-quality genome for the slender anole (Anolis apletophallus): an emerging model for field studies of tropical ecology and evolutionG3: Genes, Genomes, GeneticsDecember 29, 2023Renata M Pirani, Carlos F Arias, Kristin Charles, Albert K Chung, John David Curlis, Daniel J Nicholson, Marta Vargas, Christian L Cox, W Owen McMillan, Michael L Loganphenol–chloroform method (Barnett and Larson 2012). We purified the extracted DNA with 3× concentration of KAPA Pure Beads (Roche Sequencing) and quantified the purity using a Qubit fluorometer (Thermo Fisher Scientific). We

(3) Investigating the Human Intestinal DNA Virome and Predicting Disease-Associated Virus-Host Interactions in Severe Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS)Int J Mol SciDecember 8, 2023Shen-Yuan Hsieh, George M. Savva, Andrea Telatin, Sumeet K. Tiwari, Mohammad A. Tariq, Fiona Newberry, Katharine A. Seton, Catherine Booth, Amolak S. Bansal, Thomas Wileman, et al.of 95 °C for 10 s, 55 °C for 20 s, and 72 °C for 3 min. All libraries were then pooled, cleaned with KAPA pure beads (Roche Diagnostics Ltd., Hassocks, UK), and quantified using an Invitrogen™ Quant-iT dsDNA assay kit

(4) Comparison of Nanopore and Synthesis-Based Next-Generation Sequencing Platforms for SARS-CoV-2 Variant Monitoring in WastewaterInt J Mol SciDecember 6, 2023David Garcia-Pedemonte, Albert Carcereny, Josep Gregori, Josep Quer, Damir Garcia-Cehic, Laura Guerrero, Adrià Ceretó-Massagué, Islem Abid, Albert Bosch, Maria Isabel Costafreda, et al.control mixtures and wastewater samples were purified. The RNA controls were purified with magnetic Kapa Pure Beads (Roche, Basel, Switzerland) and the wastewater samples with the QIAquick Gel Extraction Kit (Qiagen,

(5) In-Host HEV Quasispecies Evolution Shows the Limits of Mutagenic Antiviral TreatmentsInt J Mol SciDecember 6, 2023Sergi Colomer-Castell, Josep Gregori, Damir Garcia-Cehic, Mar Riveiro-Barciela, Maria Buti, Ariadna Rando-Segura, Judit Vico-Romero, Carolina Campos, Marta Ibañez-Lligoña, Caroline Melanie Adombi, et al.elongation temperature at 50 °C and 72 °C, respectively (Figure 7). PCR product was purified using KAPA Pure Beads (Kapa Biosystems, Roche, Pleasanton, CA, USA). Quantification was carried out by Qubit fluorometry using

(6) Protocol to capture transcription factor-mediated 3D chromatin interactions using affinity tag-based BL-HiChIPSTAR ProtocolsDecember 1, 2023Ruimin Ren, Yao Hua, Heng WangI-Block Protein-Based Blocking Reagent,Invitrogen,Cat#T2015 Dynabeads MyOne Streptavidin C1,Invitrogen,Cat#65001 KAPA pure beads,KAPA,Cat#KK8001 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Gel

(7) Ecological and clinical evidence of the establishment of West Nile virus in a large urban area in Europe, Berlin, Germany, 2021 to 2022Euro SurveillDecember 1, 2023Claudia Ruscher, Corinna Patzina-Mehling, Julia Melchert, Selina L Graff, Sarah E McFarland, Christian Hieke, Anne Kopp, Anita Prasser, Torsten Tonn, Michael Schmidt, et al.final extension step at 72 °C. Resulting PCR products were pooled and purified with a twofold volume of KAPA Pure Beads according to the manufacturer’s instructions (Roche). For library preparation, the PCR Sequencing kit

(8) Whole-Genome Sequencing of 5-Hydroxymethylcytosine at Base Resolution by Bisulfite-Free Single-Step Deamination with Engineered Cytosine DeaminaseACS Central ScienceNovember 30, 2023Neng-Bin Xie, Min Wang, Wei Chen, Tong-Tong Ji, Xia Guo, Fang-Yin Gang, Ya-Feng Wang, Yu-Qi Feng, Yuan Liang, Weimin Ci, et al.a Hieff NGS Ultima DNA Ligation Module Kit (Yeasen), and the resulting DNA was purified using 0.8× KAPA Pure beads (Roche). To the resulting mixture were added DNA-L-5mC and DNA-5hmC as spike-ins to evaluate the deamination

(9) Species-specific gill’s microbiome of eight crab species with different breathing adaptationsScientific ReportsNovember 29, 2023Giovanni Bacci, Niccolò Meriggi, Christine L. Y. Cheng, Ka Hei Ng, Alessio Iannucci, Alessio Mengoni, Duccio Cavalieri, Stefano Cannicci, Sara FratiniEnzyme. PCR products were checked through electrophoresis on 1.5% agarose gel and then purified using KAPA Pure Beads (Roche) following the manufacturer’s instructions. The indexing step was performed with 2 × KAPA HiFi HotStart ReadyMix

(10) Spatial profiling of cancer-associated fibroblasts of sporadic early onset colon cancer microenvironmentnpj Precision OncologyNovember 14, 2023Satoru Furuhashi, Matias A. Bustos, Shodai Mizuno, Suyeon Ryu, Yalda Naeini, Anton J. Bilchik, Dave S. B. Hoonin equal amounts. Pooled library was purified twice with a 1.2X magnetic beads-to-sample ratio with KAPA Pure Beads (Roche, Basel, Switzerland), each followed by two 80% ethanol washes, then eluted with Elution Buffer

(11) Gut Microbiota Signatures Are Associated With Psychopathological Profiles in Patients With Ulcerative Colitis: Results From an Italian Tertiary IBD CenterInflammatory Bowel DiseasesNovember 2, 2023Franco Scaldaferri, Antonio Maria D’Onofrio, Rosaria Calia, Federica Di Vincenzo, Gaspare Filippo Ferrajoli, Valentina Petito, Eleonora Maggio, Pia Clara Pafundi, Daniele Napolitano, Letizia Masi, et al.seconds, at 72°C for 30 seconds, and a final step at 72°C for 5 minutes. DNA amplicons were cleaned up by KAPA Pure Beads (Roche Diagnostics, Mannheim, Germany). Indexed libraries were obtained by using Nextera technology

(12) Canine diffuse large B-cell lymphoma downregulates the activity of CD8 + T-cells through tumor-derived extracellular vesiclesCancer Cell InternationalOctober 26, 2023Hsin-Pei Weng, Chiao-Hsu Ke, Chun-Wei Tung, Akiyoshi Tani, Chia-Chi Wang, Wen-Yuan Yang, Yu-Shan Wang, Winston Han, Chi-Hsun Liao, Hirotaka Tomiyasu, et al.that appropriate adapter sequences were at both ends with library amplification primers. Finally, the KAPA Pure Beads system (KAPA Biosystems) was used to purify PCR products, and the library quality was estimated with

(13) Investigating Antibody Reactivity to the Intestinal Microbiome in Severe Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): A Feasibility StudyInternational Journal of Molecular SciencesOctober 18, 2023Katharine A. Seton, Marianne Defernez, Andrea Telatin, Sumeet K. Tiwari, George M. Savva, Antonietta Hayhoe, Alistair Noble, Ana L. S. de Carvalho-KoK, Steve A. James, Amolak Bansal, et al.to 50 min. DNA was precipitated using 0.7X solid phase reversible immobilisation bead clean-up with KAPA pure beads (Roche, Basel, Switzerland). Whole genome amplification was performed using the REPLI-g advanced DNA

(14) Multiple Sclerosis-Associated Gut Microbiome in the Israeli Diverse Populations: Associations with Ethnicity, Gender, Disability Status, Vitamin D Levels, and Mediterranean DietInternational Journal of Molecular SciencesOctober 9, 2023Zehavit Nitzan, Elsebeth Staun-Ram, Anat Volkowich, Ariel MillerSan Francisco, CA, USA) were used to add adaptor and index sequences, and the reactions cleaned using Kapa Pure beads (Kapa, Roche, Basel, Switzerland). The concentration of each library was measured by Qubit (Life Technologies,

(15) Comparison of Antibacterial Activity of Phytochemicals against Common Foodborne Pathogens and Potential for Selection of ResistanceMicroorganismsOctober 5, 2023Ryan Sweet, Catherine Booth, Kathryn Gotts, Stephen F. Grove, Paul A. Kroon, Mark Webberrecently [30]. Briefly, cultured cells were lysed and DNA was isolated via DNA-binding magnetic beads (KAPA Pure Beads, Roche Diagnostics) and eluted with 10 mM Tris-Cl, pH 8.5. The extracted DNA samples were sequenced

(16) Characterisation of neonatal Staphylococcus capitis NRCS-A isolates compared with non NRCS-A Staphylococcus capitis from neonates and adultsMicrobial GenomicsOctober 4, 2023Heather Felgate, Dheeraj Sethi, Kirsten Faust, Cemsid Kiy, Christoph Härtel, Jan Rupp, Rebecca Clifford, Rachael Dean, Catherine Tremlett, John Wain, et al.GloMax Discover Microplate Reader before being pooled and double-SPRI size selected between 0.5 and 0.7X bead volumes using KAPA Pure Beads (Roche). The final pool was quantified on a Qubit 3.0 instrument and run on a D5000 ScreenTape (Agilent)

(17) Single-Nucleotide Resolution Mapping of N6-Methyladenine in Genomic DNAACS Central ScienceSeptember 27, 2023Cheng-Jie Ma, Gaojie Li, Wen-Xuan Shao, Yi-Hao Min, Ping Wang, Jiang-Hui Ding, Neng-Bin Xie, Min Wang, Feng Tang, Yu-Qi Feng, et al.recommended procedures. The extracted DNA was eluted in 200 μL of H2O and further enriched by 0.4× KAPA Pure Beads (Roche). The obtained DNA was stored at −20 °C until use. Enzymatic Digestion of DNA A 30 μL mixture

(18) Uncoupling the TFIIH Core and Kinase Modules Leads To Unregulated RNA Polymerase II CTD Serine 5 PhosphorylationbioRxivSeptember 16, 2023Gabriela Giordano, Robin Buratowski, Célia Jeronimo, Christian Poitras, François Robert, Stephen Buratowskibeing used in ChIP-seq libraries. All ChIP and Input samples were subjected to a 1.7X cleanup using KAPA Pure Beads (Roche, 07983280001) according to the manufacturer’s instructions. Samples were eluted with 40 μL of

(19) Role of mucin glycosylation in the gut microbiota-brain axis of core 3 O-glycan deficient miceSci RepAugust 26, 2023Erika Coletto, George M. Savva, Dimitrios Latousakis, Matthew Pontifex, Emmanuelle H. Crost, Laura Vaux, Andrea Telatin, Kirk Bergstrom, David Vauzour, Nathalie Jugefor 30 s, 55 °C for 30 s and 72 °C for 30 s with a final step at 72 °C for 5 min. A 0.7× SPRI using KAPA Pure Beads (Roche, UK) was then performed and the DNA was eluted in 20 μL of EB (10 mM Tris–HCl). Following the

(20) Dynamics of Amino Acid Metabolism, Gene Expression, and Circulomics in a Recombinant Chinese Hamster Ovary Cell Line Adapted to Moderate and High Levels of Extracellular LactateGenes (Basel)August 2, 2023Dylan G. Chitwood, Lisa Uy, Wanfang Fu, Stephanie R. Klaubert, Sarah W. Harcum, Christopher A. Saskistarting material for a Phi-29-mediated rolling circle amplification (RCA) and magnetic bead purification (KAPA Pure Beads, Roche, KK8000) as described in the CIDER-seq protocol [37]. EccDNA libraries had SMRTbell barcodes

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KAPA Pure Beads from Roche

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KAPA Pure Beads from Roche

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