KAPA Pure Beads from Roche

Product Specs
ItemKAPA Pure Beads
CompanyRoche
Price Supplier Page View Company Product Page
Catalog Number07983271001
Quantity5 mL

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) Epigenetics and chromatin structure regulate var2csa expression and the placental binding phenotype in Plasmodium falciparumeLifeApril 15, 2026Todd Lenz, Madle Sirel, Hannes Hoppe, Sulman Shafeeq, Karine G Le Roch, Ulf Ribackeat 68°C) using the KAPA HiFi HotStart ready mix (KAPA Biosystems) before purification twice over 1X KAPA Pure beads (KAPA Biosystems). Prior to sequencing, the quality of libraries was evaluated using the Agilent 2100 Bioanalyzer

(2) Aedes aegypti Mosquitoes from Central Vietnam Feature Specific Viromic Profiles Linked to Dengue Virus CoinfectionVirusesMarch 31, 2026Margarita Popova, Alena Khalilova, Anna Gladkikh, Ekaterina Klyuchnikova, Tatiana Arbuzova, Edward Ramsay, Nguyen T. Dong, Bui T. Phu, Hung Thai Do, Vladimir DedkovRoche, Mannheim, Germany). Post-ligation clean up and post-amplification clean up were performed using KAPA Pure Beads (Roche, Mannheim, Germany). Library quality was assessed using the QIAxcel Advanced System (QIAGEN,

(3) Precise exome analysis of blastocyst biopsy scale samples using primary template-directed amplificationBMC GenomicsMarch 5, 2026Alina Samitova, Vera Belova, Iuliia Vasiliadis, Zhanna Repinskaia, Tatiana Gorodnicheva, Evgeny Romanov, Mariam Pogosyan, Emil Gaysin, Tatyana Nazarenko, Denis Rebrikov, et al.[902.3–2061.8] ng; range 516–3240 ng) of DNA per sample after PTA-WGA and further purification using 2x Kapa Pure Beads (Roche). Raw sequencing data and pooling balance We obtained between 190 and 249 million (M) reads

(4) Genome-wide DNA methylation profiling in COVID-19 positive patients reveals alterations in pathways linked to neurological dysfunctionClinical EpigeneticsFebruary 27, 2026Saima Zameer, Ehraz Anis, Qiong Sha, Martha L. Escobar Galvis, Sanam Khan, Jennifer A. Steiner, Milda Milčiūtė, Ieva Kerševičiūtė, Migle Gabrielaite, Juozas Gordevicius, et al.denaturation was performed using 0.1 N sodium hydroxide, followed by four cycles of PCR amplification. KAPA pure beads (Roche Sequencing, Cat. #KK8001) were used for cleanup steps in all prepared libraries. Quality and

(5) Dose-dependent IFN programs in myeloid cells after mRNA and adenovirus COVID-19 vaccinationJCI InsightFebruary 23, 2026Giray Eryilmaz, Yilmaz Yucehan Yazici, Radu Marches, Eleni P. Mimitou, Lisa Kenyon-Pesce, Kim Handrejk, Sonia Jangra, Michael Schotsaert, Adolfo García-Sastre, George A. Kuchel, et al.were ligated with xGen UDI-UMI adapters for Illumina (IDT). Adapter-ligated DNA was purified using KAPA Pure beads (Roche). This was followed by 12 cycles of PCR amplification and another clean up. Purified libraries

(6) Hookworm genomic diversity and population structure from accessible sample types: A validated approach to generate genome-wide polymorphism datasets from individual third-stage larvaebioRxivFebruary 20, 2026Kaylee S Herzog, Savanna Randi, Dickson Osabutey, Christina Paraggio, Richard Bungiro, Lisa Harrison, Irene S Offei Owusu, Francis Appiah-Tsum, Amanda Lamptey, Isaac Quaye, et al.instructions, and eight L3s were extracted per buffer. Following lysis, extractions were purified using KAPA Pure Beads (Roche) in a 1:1 bead-to-sample ratio and eluted in 25μL nuclease-free water. All extractions were quantified

(7) Gut microbial diversity impacts carbohydrate fermentation by children with severe acute malnutritioniScienceFebruary 20, 2026Akshay Bisht, Jennifer Ahn-Jarvis, Kendall Corbin, Suzanne Harris, Perla Troncoso-Rey, Peter Olupot-Olupot, Nuala Calder, Kevin Walsh, Kathryn Maitland, Gary Frost, et al.for 30 s, followed by a final 720C for 5 min. Following PCR, a 0.7X SPRI clean-up was performed using KAPA Pure Beads (Roche Catalogue No. 07983298001), eluting the DNA in 20 μL of water. A second PCR master mix was made

(8) Dynamic chromatin accessibility and transcriptional landscapes of porcine kidney cells during pseudorabies virus infectionFrontiers in ImmunologyFebruary 4, 2026Songbai Yang, Mingyang Dong, Haixin Shi, Xiangchen Li, Han Wang, Xiaolong Zhou, Ayong Zhao72 °C for 30 s, and a final extension at 72 °C for 5 m. The amplified products were purified using KAPA Pure Beads (Cat# KS8002, KAPA Biosystems), and fragment selection was performed. The library fragment size and

(9) Bile acids regulate lipid metabolism through selective actions on fatty acid absorptionCell MetabolismFebruary 3, 2026Alvin P. Chan, Kelsey E. Jarrett, Rochelle W. Lai, Madelaine C. Brearley-Sholto, Angela S. Cheng, Maria O. Taveras, Anne M. Iwata, Michelle E. Steel, Andrew Lau, Emily C. Whang, et al.were pooled to yield 1000 ng of final pooled product, then cleaned up using KAPA Pure Beads (Roche, 07983271001) for library size selection, which removed fragments shorter than 250 bp and longer than 1,500 bp. The

(10) Mycorrhizal fungal associations of the fringed orchids (Platanthera) in the US, inter- and intra-species variationBiodiversity and ConservationFebruary 1, 2026Melissa K. McCormick, Ida Hartvig, Simone A. Evans, Julianne McGuinness, Stephanie Lyon, Zöe Knapp, Jason Ligon, Lauren Eserman, Emily Coffey, Dennis F. Whighampooled together in equimolar concentration. A final cleanup was conducted on the resulting library using KAPA Pure Beads (Roche), with a 0.8X bead to library ratio. The library was analyzed on an Agilent TapeStation 4200

(11) Multiomics transcriptome and metabolome insights into fat metabolism and meat quality in Songliao black pigs and Songlei crossbred pigsBMC GenomicsJanuary 22, 2026Jing Xu, Yunpeng Zhang, Suthar Teerath Kumar, Enlong Liu, Yunlong Zheng, Yalong Zhu, Qi Zhang, Wu-Sheng Sun, Li Pan, Yuan Zhao, et al.the primer sequences on the adapters. The amplified PCR products were purified and recovered using KAPA Pure Beads (KAPA BIOSYSTEMS, United States) for fragment selection. Library fragment size and concentration were

(12) Genomic characterization of a severe West Nile Virus transmission season using a single reaction amplicon sequencing approachPLOS Neglected Tropical DiseasesJanuary 21, 2026Shawn Freed, Sarah Chandler, Sarah Uhm, Zach Pella, Dikchha Gurung, Hallie Smith, Tammy Dowdy, Amanda M. Bartling, Ava Butz, Michael R. Wiley, et al.previously described [24] with reduced PCR cycles to 30. Following bead-based sample purification using KAPA Pure Beads (Roche), samples were eluted into 25ul molecular grade water. Pool 1 and Pool 2 contents were pooled

(13) Epigenetics and chromatin structure regulate var2csa expression and the placental binding phenotype in Plasmodium falciparumbioRxivJanuary 16, 2026Todd Lenz, Madle Sirel, Hannes Hoppe, Sulman Shafeeq, Karine Le Le Roch, Ulf Ribackeat 68 °C) using the KAPA HiFi HotStart ready mix (KAPA Biosystems) before purification twice over 1X KAPA Pure beads (KAPA Biosystems). Prior to sequencing, quality of libraries was evaluated using Agilent 2100 Bioanalyzer

(14) The human IG heavy chain constant gene locus is enriched for large structural variants and coding polymorphisms that vary among human populationsCell GenomicsJanuary 14, 2026Uddalok Jana, Oscar L. Rodriguez, William Lees, Eric Engelbrecht, Zach Vanwinkle, Ayelet Peres, William S. Gibson, Kaitlyn Shields, Steven Schultze, Abdullah Dorgham, et al.Biosciences,Cat#100-265-900 "Barcode adapters, DNA Plate Duplex Oligo, 50 μM",IDT,IDT Plate #13748866 "KAPA Pure Beads, 60 mL",KAPA Roche,Cat#07983298001 "Universal Oligos, 100 μM",IDT,Ref#288647790 KAPA Probes Resuspension

(15) Combined metabolomic and metagenomic analysis reveals inflammatory bowel disease diversity in pediatric and adult patientsWorld J GastroenterolDecember 28, 2025Natalya B Zakharzhevskaya, Svetlana I Erdes, Elena A Belousova, Ivan S Samolygo, Marina A Manina, Polina V Kondrashova, Ekaterina Y Lomakina, Dmitry A Kardonsky, Elizaveta A Vorobyeva, Olga Y Shagaleeva, et al.(SQK-NBD109.96)) were ligated using Blunt/TA Ligase Master Mix (NEB). Barcoded libraries were purified using KAPA Pure Beads (Roche, Switzerland). Library concentrations were measured using the Quant-iT dsDNA Assay Kit, High Sensitivity

(16) Menin-MLL1 complex cooperates with NF-Y to promote hepatocellular carcinoma survivalCell ReportsDecember 23, 2025Margarita Dzama-Karels, Mallory Sokolowski, Peyton Kuhlers, Jacqueline A. Brinkman, John P. Morris, Jesse R. Raabadapter concentration (Roche Dual Index, 08861919702). Libraries were cleaned twice using 1.1× volumes Kapa pure beads before PCR amplification using Kapa Hifi PCR mix with the following conditions: 98°C for 30″ to denature

(17) Identification of Sjögren’s disease–associated T cell receptor motifs through deep sequencingJCI InsightDecember 22, 2025Ananth Aditya Jupudi, Michelle L. Joachims, Christina Lawrence, Charmaine Lopez-Davis, Bhuwan Khatri, Astrid Rasmussen, Kiely Grundahl, R. Hal Scofield, Judith A. James, Joel M. Guthridge, et al.Adaptor-tagged DNA from pooled samples was subjected to size selection, and excess adaptors were removed using KAPA Pure beads (Roche Sequencing Solutions). Following a PCR amplification step using Illumina universal index primers,

(18) EpCAM activates the ERK-EGR1 signaling axis and promotes TNF-α-induced the progression of anaplastic thyroid cancerJournal of Translational MedicineDecember 22, 2025Chi-Chiu Lee, Yi-Jen Su, Sushree Shankar Panda, Chiung-Yi Chiu, Chung-Hsuan Wu, Tsai-Ming Lu, Pi-Chun Li, Yen-Lin Huang, Ruey-Long Hong, Shao-Hsi Hung, et al.carrying the adapters. To select cDNA fragments of 300–400 bp, the library fragments were purified with KAPA Pure Beads system (KAPA Biosystems, Roche, Basel, Switzerland). The library carrying appropriate adapter sequences

(19) A Monitoring Method to Evaluate the Accumulation of Antimicrobial-Resistance Genes in Gram-Negative Bacteria Distributed in Environmental WaterEnviron Microbiol RepDecember 19, 2025Nobuyoshi Yagi, Sora Miyazato, Nguyen Quoc Anh, Bui Thi Mai Huong, Itaru Hiraimin 45 s, and a final extension at 72°C for 7 min. Then the amplified DNA samples were purified by KAPA Pure Beads (Roche Sequencing Solutions Inc., Pleasanton, CA) and subjected to the Nanopore sequencing (Oxford Nanopore

(20) Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbationNature CommunicationsDecember 12, 2025Rajini Srinivasan, Tao Sun, Azalia Sandles, Diana Wu, Liang Wang, Hetal Patel, Ryan Pabalate, Maram Bader, Amy Heidersbach, Clark Ho, et al.amplicons from all of the reactions are pooled together. The amplicon library pool is cleaned twice using KAPA Pure Beads (Kapa) at a 1:1 ratio to remove unused primers and index adapters. The quality and quantity of the clean

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KAPA Pure Beads from Roche

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KAPA Pure Beads from Roche

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