KAPA Pure Beads from Roche

Product Specs
ItemKAPA Pure Beads
CompanyRoche
Price Supplier Page View Company Product Page
Catalog Number08963851001
Quantity60 mL

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) Protocol for flash-freezing, pooling, and preparing single nuclei suspensions from amniote inner-ear sensory epitheliaSTAR ProtocAugust 18, 2026Jimena Perez Lloret, Valeria Carolina Castagna, Marcela LipovsekBetaine solution,Sigma-Aldrich,B0300-1VL Human kidney total RNA,ThermoFisher,QS0616 KAPA HiFi HotStart ReadyMix PCR Kit,Roche,KK2601 Kapa® Pure Beads,Roche,KK8000 Buffer EB,Qiagen,19086 Qubit 1X dsDNA HS Assay Kit,ThermoFisher,Q33230 Bioanalyzer High

(2) Gut microbiome and functional adaptations to paleo and rational diets in professional athletes: a pilot studyFront MicrobiolAugust 14, 2026Aleksandra Pięta, Kinga Zielińska, Kinga Humińska-Lisowska, Barbara Frączekconcentration to limit the formation of adapter dimers. After ligation, the libraries were purified with KAPA Pure Beads (Roche, Switzerland) and six PCR cycles were performed to amplify the libraries with minimal bias. A

(3) Complex landscape of somatic copy number alterations in head and neck paragangliomasFront Endocrinol (Lausanne)August 14, 2026Vladislav S. Pavlov, Maria S. Fedorova, Turpal-Ali S.M. Elnukaev, Dmitry V. Kalinin, Elena A. Pudova, Irina V. Katunina, Zulfiya G. Guvatova, Anastasia A. Kobelyatskaya, Andrey D. Kaprin, Anna V. Kudryavtseva, et al.min. The sizes of the PCR products were verified on a 2% agarose gel and subsequently purified using KAPA Pure Beads (Roche, Switzerland). Fragment analysis of the amplicons was performed on a NANOFOR-05 instrument (Sintol,

(4) Molasses-amended siderophore biofertilizer reshapes the rhizosphere microbiome, promotes growth and reduces lipid peroxidation in Ocimum basilicum LFront Plant SciAugust 13, 2026Marcin Musiałowski, Anna Bernatowicz, Łucja Kowalewska, Aleksandra Goszcz, Xiaomeng Chen, Silvia Celletti, Michela Schiavon, Klaudia Dębiec-Andrzejewskafor 45 s, followed by a final elongation at 72 °C for 1 min. Obtained DNA material was purified using KAPA Pure Beads (Roche, Switzerland) and quantified with Qubit fluorometer. Sequencing libraries were prepared with

(5) Measuring the change in deleterious mutation burden of barley germplasm conserved over one regeneration cycleG3: Genes, Genomes, GeneticsAugust 5, 2026Yong-Bi Fuquality, the multiplex library was further cleaned to remove small fragments (<250 bp) using 2 rounds of KAPA Pure Beads (Roche Canada, Mississauga, ON, Canada) at a 0.9× beads-to-sample ratio, following the single-tail clean-up

(6) Microbial communities in the rhizosphere of three Mentha species: Links to soil properties and essential oil profilesPLoS OneJuly 31, 2026Yasmine Wazzani, Szilvia Tavaszi-Sárosi, Katalin Patonay, Ferenc Olasz, Ákos Juhász, Katalin PostaHotStart Ready Mix, with 8 amplification cycles. Indexed libraries were purified using 0.8 × volume KAPA Pure Beads (Roche AG, Basel, Switzerland). Library quantification was performed with the Equalbit 1 × dsDNA HS

(7) Genetic Elements Associated with the Acquired Resistome of the Gut Microbiota in a Broiler Rooster Flock in HungaryAnimals (Basel)July 29, 2026János Kiss, Balázs Libisch, Chioma Lilian Ozoaduche, Hedvig Fébel, Geertrui Rasschaert, Ellen Lambrecht, Marc Heyndrickx, Mónika Szabó, Tibor Keresztény, Katalin Posta, et al.Basel, Switzerland) in a 25-cycle PCR1 reaction. The PCR products were purified with 0.8 volume of KAPA Pure Beads (Roche, Basel, Switzerland). Then, 10 ng of PCR1 products was used as a template in index PCRs. Indexing

(8) Operationalising Environmental DNA (eDNA) Detection of Major Malaria Vector Species in GhanaMol Ecol ResourJuly 28, 2026Mojca Kristan, Yaw A. Afrane, Sophie Moss, Yaw Akuamoah-Boateng, Abdul Rahim Mohammed Sabtiu, Judith Dzifa Azumah, Anisa Abdulai, Isaac Kwame Sraku, Bright Churchill Obeng, Karen L. Figueroa Chilito, et al.and size. Barcoded PCR products from different eDNA samples were pooled. Pools were purified using KAPA pure beads (Roche Diagnostics, USA) at a ratio of 0.8:1 (beads: DNA) to remove PCR reagents and excess primers.

(9) Type V Collagen Controls Decidual Extracellular Matrix Organization and Angiogenesis During Embryo ImplantationbioRxivJuly 25, 2026Mona Gebril, Emily Kinneston, Rimi Das, Joseph Boyd, John P Lydon, Shanmugasundaram Nallasamywere ligated to the libraries, and products were purified using KAPA Pure Beads (KAPA/Roche, Cat. No. 07983298001). Libraries were then amplified, bead-purified, and eluted in 20 µL of 10 mM Tris-Cl, pH 8.5 (Qiagen,

(10) Proteome‐Wide Prediction of Putative Bemisia tabaci Effector Candidates and Transient Validation of BtApe‐Mediated Suppression of Tomato Immune ResponsesArchives of Insect Biochemistry and PhysiologyJuly 1, 2026Prakash Kolanchi, Murugan Marimuthu, Mohana Pradeep Rangaraj Kumar, Vamsi Nalam, Punya Nachappapoly(A) RNA enrichment, reverse transcription, and adapter ligation. Libraries were purified using KAPA Pure Beads to remove adapter dimers and other contaminants, and fragment size distribution was validated using

(11) Cannabidiol Activates Integrated Stress Response Signaling and Immune Trafficking Programs in an A375 Melanoma–Jurkat T Cell Coculture Model: A Multi-Omics AnalysisACS OmegaJune 30, 2026Ni Deng, Huifang Li, Ang Cai, Christopher L. Hemme, Jonghae Youn, Ryan Janis, Matthew D. Stone, Hang Ma, Chang Liuindexes (7 μM; KAPA/Roche, 8861919702) were added via ligation, and the product was purified using KAPA Pure Beads (KAPA/Roche, 7983298001). The purified product was then amplified, bead-purified, and finally, eluted

(12) Uncoupling the TFIIH Core and Kinase Modules leads to misregulated RNA polymerase II CTD Serine 5 phosphorylationElifeJune 8, 2026Gabriela Giordano, Robin Buratowski, Célia Jeronimo, Christian Poitras, François Robert, Stephen Buratowskibeing used in ChIP-seq libraries. All ChIP and Input samples were subjected to a 1.7× cleanup using KAPA Pure Beads (Roche, 07983280001) according to the manufacturer’s instructions. Samples were eluted with 40 μL of

(13) Adhesion-derived condensates control component availability to regulate adhesion dynamicsNature CommunicationsJune 5, 2026Michal Dibus, Megan R. Chastney, Giray Enkavi, Gautier Follain, Omkar Joshi, Ilpo Vattulainen, Johanna Ivaskain Supplementary Data 1). PCR was carried out for 18 cycles, and the reactions were purified using KAPA Pure Beads (Roche), digested with DpnI (Thermo Fisher Scientific) and transformed into E. coli DH5α competent bacteria.

(14) When clades collide: Genomic admixture in blacklegged ticks (Ixodes scapularis) from the Great PlainsbioRxivJune 4, 2026Kaylee S. Herzog, Zach Pella, Halie Smith, Dayna McCormick, Lily Nuss, Amanda Brinkworth, Sujata Chaudhari, Kathryn Duncan, Kellee Sundstrom, Sydney Stein, et al.by McClung et al (2023)33. Prior to library preparation, these extractions were bead-cleaned using KAPA Pure Beads (Roche) in a 1:1 ratio, eluted in nuclease-free water, and quantified using Qubit™ dsDNA HS assay. Next-generation sequencing

(15) Diversity and community structure of anaerobic gut fungi in camelsbioRxivMay 29, 2026G. L. Sakalya N. H. De Silva, julia Vinzelj, Samuel L. Miller, Anna M. Jemmett, Mostafa S. Elshahed, Noha H. Yousseffollowing the manufacturer’s instructions. The indexed PCR products were subsequently purified again using KAPA Pure Beads (Kapa Biosystems). Samples were then normalized and pooled using the Illumina Library Pooling Calculator

(16) Allele-specific antisense oligonucleotide treatment rescues atad3- associated phenotype in zebrafishbioRxivMay 27, 2026Shlomit Ezer, Shira Yanovsky-Dagan, Amit Granit, Matthew McDougal, Taeyoung Hwang, Israel Antman, Rotem Karni, Wan Hee Yoon, Ann Saada, Adi Inbal, et al.attach sample-specific index sequences (IDT, USA) to the adapter. PCR products were purified using KAPA Pure Beads (Roche, Switzerland) and diluted to a normalized concentration of 0.1ng/ul. Samples were sequenced on

(17) Arginine methylation of BAF155 regulates interactions with RNA processing machinerybioRxivMay 22, 2026Mallory Sokolowski, Deena Scoville, Peyton C. Kuhlers, Jesse R. Raabamplification was performed accordingly, and libraries were purified using a two-sided bead cleanup (KAPA Pure Beads, Roche) with 0.6x and 1.2x ratios to remove large fragments and unincorporated adapters. Library concentration

(18) Mitochondrial l-2-hydroxyglutarate is a physiological signalling metaboliteNatureMay 20, 2026Ram P. Chakrabarty, Jonathan G. Van Vranken, Yuki Aoi, Taylor A. Poor, Gregory S. McElroy, Karthik Vasan, Shimaa H. A. Soliman, Marta Iwanaszko, Rogan A. Grant, Benjamin C. Howard, et al.HotStart Ready Mix master mix (KAPA, KR0370). To remove small fragments, we performed size selection using KAPA pure beads: 40 µl KAPA beads was added to 50 µl of the PCR solution. We ran 2 µl of the library on a TapeStation

(19) An engineered viral RNA degrader on mitochondrial surface that mitigates RNA virus infectionNature CommunicationsMay 19, 2026Chih-Wei Chen, Wei-Ting Liao, Tung Chao, Yi-Han Chen, Shih-Che Weng, Shin-Hong Shiao, Ching-Yang Kao, Yun-Jung Li, Kai-An Cheng, Zee-Fen Changthe manufacturer’s recommendations to remove ribosomal RNA. The libraries were then purified using KAPA Pure Beads system and assessed on the Qsep 100 DNA/RNA Analyzer (BiOptic Inc., Taiwan) for quality check. The PCR

(20) An autochthonous CRISPR activation screening platform for characterizing tissue-specific oncogene selectionCell Reports MedicineMay 19, 2026Fredrik I. Thege, Amber Hoskins, Annaliese Kramer, Intisar Salim, Ashwath Seetharaman, Natalie Fowlkes, Kimal I. Rajapakshe, Anirban Maitra, Sonja M. WoermannNEBuilder HiFi DNA Assembly Master Mix,NEB,E2621S GlycoBlue Coprecipitant,Thermofisher,AM9515 NEBNext Ultra II Q5 Master Mix,NEB,M0544L KAPA Pure Beads,Roche,7983280001 NextSeq 1000/2000 P1 Reagents (100 cycles),Illumina,20074933 ,, Critical commercial

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KAPA Pure Beads from Roche

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