KAPA Pure Beads from Roche

Product Specs
ItemKAPA Pure Beads
CompanyRoche
Price Supplier Page View Company Product Page
Catalog Number07983298001
Quantity60 mL

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) An autochthonous CRISPR activation screening platform for characterizing tissue-specific oncogene selectionCell Reports MedicineMay 19, 2026Fredrik I. Thege, Amber Hoskins, Annaliese Kramer, Intisar Salim, Ashwath Seetharaman, Natalie Fowlkes, Kimal I. Rajapakshe, Anirban Maitra, Sonja M. WoermannNEBuilder HiFi DNA Assembly Master Mix,NEB,E2621S GlycoBlue Coprecipitant,Thermofisher,AM9515 NEBNext Ultra II Q5 Master Mix,NEB,M0544L KAPA Pure Beads,Roche,7983280001 NextSeq 1000/2000 P1 Reagents (100 cycles),Illumina,20074933 ,, Critical commercial

(2) Comparative Genomics of Transisthmian Damselfishes ( Abudefduf saxatilis and A. troschelii )Genome Biology and EvolutionMay 1, 2026Claire B Tracy, Carlos F Arias, Eirlys Tysall, Marc P Hoeppner, Owen W McMillan, Oscar Puebla, Moisés A Bernalmuscle tissue using a phenol:chloroform method (Sambrook and Russell 2006). The DNA was cleaned with 3X KAPA pure Beads (Roche Sequencing) and concentration/purity of the extracted DNA was assessed using a Qubit fluorometer

(3) Exploring the diversity and community structure of the Testudines fecal mycobiomebioRxivApril 27, 2026Taylor Mills, Julia M. Vinzelj, Emma R. Cook, Emma Mills, Alexander J. Rurik, Jason W. Dallas, Donald M. Walker, Paul A. Stone, Cameron D. Siler, Mostafa S. Elshahed, et al.were then cleaned to remove primers as well as any non-specific PCR products shorter than 200 bp using KAPA Pure Beads (Kapa Biosystems). Cleaned products were individually quantified and pooled using the Illumina library

(4) Intron retention regulates STAT2 function and predicts immunotherapy response in lung cancerbioRxivApril 21, 2026Ryan P. Englander, Mattia Brugiolo, Te-Chia Wu, Mitch Kostich, Nathan K. Leclair, SungHee Park, Jacques Banchereau, Peter Yu, Andrew Salner, Romain Banchereau, et al.fragments were ligated with Illumina unique adaptors (Illumina). Adaptor-ligated DNA was purified using KAPA Pure beads. This was followed by 10 cycles of PCR amplification. The final library was cleaned up using KAPA Pure Beads

(5) Epigenetics and chromatin structure regulate var2csa expression and the placental binding phenotype in Plasmodium falciparumeLifeApril 15, 2026Todd Lenz, Madle Sirel, Hannes Hoppe, Sulman Shafeeq, Karine G Le Roch, Ulf Ribackeat 68°C) using the KAPA HiFi HotStart ready mix (KAPA Biosystems) before purification twice over 1X KAPA Pure beads (KAPA Biosystems). Prior to sequencing, the quality of libraries was evaluated using the Agilent 2100 Bioanalyzer

(6) Epigenetics and chromatin structure regulate var2csa expression and the placental-binding phenotype in Plasmodium falciparumElifeApril 15, 2026Todd Lenz, Madle Sirel, Hannes Hoppe, Sulman Shafeeq, Karine G Le Roch, Ulf Ribackeat 68°C) using the KAPA HiFi HotStart ready mix (KAPA Biosystems) before purification twice over 1X KAPA Pure beads (KAPA Biosystems). Prior to sequencing, the quality of libraries was evaluated using the Agilent 2100 Bioanalyzer

(7) Peaked-to-flat transition in quasispecies structure evolutionVirus EvolutionApril 14, 2026Josep Gregori, Sergi Colomer-Castell, Carolina Campos, Marta Ibañez-Lligoña, Damir García-Cehic, Maria Francesca Cortese, David Tabernero, Mar Riveiro-Barciela, Maria Buti, Ariadna Rando-Segura, et al.(ThermoFisher Scientific, Waltham, MA, USA). All amplicons were normalized to the same concentration and purified using KAPA Pure Beads magnetic beads (KAPA Biosystems; Roche Applied Science, Pleasanton, CA, USA). A second quantification

(8) Defined cellular reprogramming of androgen receptor-active prostate cancer to neuroendocrine prostate cancerbioRxivApril 11, 2026Shan Li, Kai Song, Huiyun Sun, Yong Tao, Arthur Huang, Vipul Bhatia, Brian Hanratty, Radhika A. Patel, Henry W. Long, Colm Morrissey, et al.to targted PCR amplification and PCR products were removed from individual droplets, purified with KAPA Pure Beads (Roche Molecular Systems), and used as a template for PCR to incorporate Illumina P7 indices. PCR products

(9) Sgk1 upregulation in hippocampus-projecting amygdala neurons underlies the delayed onset of PTSD-like avoidance behaviorNature CommunicationsApril 1, 2026Jia-Xin Zou, Wei-Zhu Liu, Ya-Qing Li, Yuan-Yuan Li, Wen-Jie You, Han-Qing Pan, Chun-Yan Wang, Wen-Hua Zhang, Bing-Xing Panmin, 20 cycles; Step3: 72 °C for 5 min; Step4: hold at 4 °C. The cDNA products were purified using KAPA Pure Beads (Roche, Basel, Switzerland). The RT-qPCR was conducted on StepOnePlus Real-Time PCR System (Thermo Fisher Scientific

(10) Aedes aegypti Mosquitoes from Central Vietnam Feature Specific Viromic Profiles Linked to Dengue Virus CoinfectionVirusesMarch 31, 2026Margarita Popova, Alena Khalilova, Anna Gladkikh, Ekaterina Klyuchnikova, Tatiana Arbuzova, Edward Ramsay, Nguyen T. Dong, Bui T. Phu, Hung Thai Do, Vladimir DedkovRoche, Mannheim, Germany). Post-ligation clean up and post-amplification clean up were performed using KAPA Pure Beads (Roche, Mannheim, Germany). Library quality was assessed using the QIAxcel Advanced System (QIAGEN,

(11) Using Carrier DNA in Ultra-Low Input Library Preparations for Next-Generation SequencingJournal of Biomolecular TechniquesMarch 6, 2026Ping Li, Jeremy Kahsen, Karen Olsson-Francis, Stefan J. Greenindicate 15–16 cycles (library amplification stage) for 50 pg inputs. Library pools were purified using KAPA Pure Beads (Roche, Basel, Switzerland), quantified using a Qubit4 Fluorometer with the Qubit dsDNA HS Assay Kit

(12) Precise exome analysis of blastocyst biopsy scale samples using primary template-directed amplificationBMC GenomicsMarch 5, 2026Alina Samitova, Vera Belova, Iuliia Vasiliadis, Zhanna Repinskaia, Tatiana Gorodnicheva, Evgeny Romanov, Mariam Pogosyan, Emil Gaysin, Tatyana Nazarenko, Denis Rebrikov, et al.[902.3–2061.8] ng; range 516–3240 ng) of DNA per sample after PTA-WGA and further purification using 2x Kapa Pure Beads (Roche). Raw sequencing data and pooling balance We obtained between 190 and 249 million (M) reads

(13) Human TET2-mutant clonal hematopoiesis expansion is driven by distinct inflammatory signaling responses in stem cells versus myeloid progenyBlood Cancer DiscovMarch 4, 2026Hector Huerga Encabo, Giuseppe D'Agostino, Katherine Sturgess, Alice E. Lord, Eric D. Jong, Alessandra Ferrelli, Aneesh Sharma, Syed A. Mian, Khadidja Habel, Miriam Llorian-Sopena, et al.Library PCR amplification was done with 9 cycles and double-sided size selection was performed using KAPA Pure beads (Roche, 07983280001). Sequencing was then performed in a HiSeq4000 Sequencing System (Illumina) with

(14) Genome-wide DNA methylation profiling in COVID-19 positive patients reveals alterations in pathways linked to neurological dysfunctionClinical EpigeneticsFebruary 27, 2026Saima Zameer, Ehraz Anis, Qiong Sha, Martha L. Escobar Galvis, Sanam Khan, Jennifer A. Steiner, Milda Milčiūtė, Ieva Kerševičiūtė, Migle Gabrielaite, Juozas Gordevicius, et al.denaturation was performed using 0.1 N sodium hydroxide, followed by four cycles of PCR amplification. KAPA pure beads (Roche Sequencing, Cat. #KK8001) were used for cleanup steps in all prepared libraries. Quality and

(15) CD146 + interstitial cells contribute to the dystrophic skeletal muscle phenotype in vitroScientific ReportsFebruary 24, 2026Bartosz Mierzejewski, Zuzanna Michalska, Dominika Kulma, Aleksandra Bos, Wladyslawa Streminska, Zbigniew Bartoszewicz, Edyta Brzoskafrom cultured WT or mdx CD146 + cells was purified using KAPA Magnetic Beads (KAPA Biosystems, cat no. 07983298001) and then subjected to ribodepletion and library construction using the KAPA RNA HyperPrep Kit with

(16) Dose-dependent IFN programs in myeloid cells after mRNA and adenovirus COVID-19 vaccinationJCI InsightFebruary 23, 2026Giray Eryilmaz, Yilmaz Yucehan Yazici, Radu Marches, Eleni P. Mimitou, Lisa Kenyon-Pesce, Kim Handrejk, Sonia Jangra, Michael Schotsaert, Adolfo García-Sastre, George A. Kuchel, et al.were ligated with xGen UDI-UMI adapters for Illumina (IDT). Adapter-ligated DNA was purified using KAPA Pure beads (Roche). This was followed by 12 cycles of PCR amplification and another clean up. Purified libraries

(17) Hookworm genomic diversity and population structure from accessible sample types: A validated approach to generate genome-wide polymorphism datasets from individual third-stage larvaebioRxivFebruary 20, 2026Kaylee S Herzog, Savanna Randi, Dickson Osabutey, Christina Paraggio, Richard Bungiro, Lisa Harrison, Irene S Offei Owusu, Francis Appiah-Tsum, Amanda Lamptey, Isaac Quaye, et al.instructions, and eight L3s were extracted per buffer. Following lysis, extractions were purified using KAPA Pure Beads (Roche) in a 1:1 bead-to-sample ratio and eluted in 25μL nuclease-free water. All extractions were quantified

(18) Gut microbial diversity impacts carbohydrate fermentation by children with severe acute malnutritioniScienceFebruary 20, 2026Akshay Bisht, Jennifer Ahn-Jarvis, Kendall Corbin, Suzanne Harris, Perla Troncoso-Rey, Peter Olupot-Olupot, Nuala Calder, Kevin Walsh, Kathryn Maitland, Gary Frost, et al.5 min. Following PCR, a 0.7X SPRI clean-up was performed using KAPA Pure Beads (Roche Catalogue No. 07983298001), eluting the DNA in 20 μL of water. A second PCR master mix was made up using 4 μL KAPA 2G buffer,

(19) Dynamic chromatin accessibility and transcriptional landscapes of porcine kidney cells during pseudorabies virus infectionFrontiers in ImmunologyFebruary 4, 2026Songbai Yang, Mingyang Dong, Haixin Shi, Xiangchen Li, Han Wang, Xiaolong Zhou, Ayong Zhao72 °C for 30 s, and a final extension at 72 °C for 5 m. The amplified products were purified using KAPA Pure Beads (Cat# KS8002, KAPA Biosystems), and fragment selection was performed. The library fragment size and

(20) Bile acids regulate lipid metabolism through selective actions on fatty acid absorptionCell MetabolismFebruary 3, 2026Alvin P. Chan, Kelsey E. Jarrett, Rochelle W. Lai, Madelaine C. Brearley-Sholto, Angela S. Cheng, Maria O. Taveras, Anne M. Iwata, Michelle E. Steel, Andrew Lau, Emily C. Whang, et al.(Illumina, 20091654). Libraries were pooled to yield 1000 ng of final pooled product, then cleaned up using KAPA Pure Beads (Roche, 07983271001) for library size selection, which removed fragments shorter than 250 bp and longer

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KAPA Pure Beads from Roche

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