Resin from Promega

Product Specs
ItemResin
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA1753
Quantity5.5ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Urine-based diagnostic tests for tuberculosis: a scoping review highlighting unmet diagnostic needsFrontiers in MicrobiologyMarch 27, 2026Mayu Hikone, Yuya Kondo, Yu Takaizumi, Wataru Kagaya, Katharina Kranzer, Nobuo Saito°C",NR,"EDTA, Tris–HCl","4,000 g, 20 min, pellet/supernatant",Guanidine isothiocyanate; silica-based Wizard Resin (Promega),IS6110,129 (67),"Positivity: PTB (MRS+) 79.7% (n = 43), other 0% (n = 10), healthy 0% (n =

(2) Unveiling the viral frontier in a warming world: temperature as a key ecological driver of viral diversity in subantarctic Chilean Patagonia fjordsEnviron MicrobiomeMarch 3, 2026Marianne Buscaglia, Wilson Castillo-Inaipil, Frederik Schulz, Sergio Guajardo-Leiva, José Luis Iriarte, Pilar Aparicio-Rizzo, Italo Masotti, Beatriz DíezAmicon ultracentrifugal filters before DNA extraction with Wizard columns (Wizard DNA Purification Resin (Promega #A7181) and Wizard Mini Columns (Promega # A7211)). DNA from both size fractions was sequenced

(3) Structural Mechanisms of SAMD9 Autoinhibition and Pathogenic DysregulationbioRxivFebruary 3, 2026Zongjun Mou, Fushun Zhang, Marisol Morales, Bibekananda Sahoo, Xinghong Dai, Yan XiangProtein Purification Buffer. Binding was performed by inversion mixing at 4°C for ≥5 h (typically overnight). Resin was washed three times with HaloTag Protein Purification Buffer containing IGEPAL and three additional

(4) Inhibitors supercharge kinase turnover through native proteolytic circuitsNatureJanuary 22, 2026Natalie S. Scholes, Martino Bertoni, Arnau Comajuncosa-Creus, Katharina Kladnik, Xuefei Guo, Fabian Frommelt, Matthias Hinterndorfer, Hlib Razumkov, Polina Prokofeva, Martin P. Schwalm, et al.day, the beads were centrifuged briefly (30 s) and the supernatant was transferred into a fresh tube. Resin was washed once using 200 µl H2O, which was added to the already separated supernatant. Peptides were

(5) Carbonic Anhydrase 3 Overexpression Modulates Signalling Pathways Associated with Cellular Stress Resilience and ProteostasisInt J Mol SciDecember 15, 2025Yezhou Yu, Merrina Anugraham, Tony Blick, Arutha Kulasinghe, Louise M. Sternicki, Giovanna Di Trapani, Sally-Ann Poulsen, Daniel Kolarich, Kathryn F. Tonissen(Promega). HaloLink Resin (200 µL, Promega) was spun down (800× g, 1 min) and washed with 800 µL of Resin Equilibration/Wash Buffer (Promega) for a total of 3 washes. The resin was centrifuged at 800× g for

(6) Multi-domain temporal patterns reveal stable community membership but dynamic interactions in the coastal microbiomeEnvironmental MicrobiomeDecember 10, 2025Elvira Rey Redondo, Wenqian Xu, Yangbing Xu, Ruixian Sun, Siu Hei Wan, Shara K. K. Leung, Charmaine C. M. Yung(Zymo Research, USA), while viral DNA was extracted from 1 μm filters using Wizard DNA Purification Resin columns (Promega, USA). The extracted genomic DNA and total RNA were sent to Novogene (Tianjin, China)

(7) Condensation-dependent interactome of a chromatin remodeler underlies tumor suppressor activitiesNat CommunOctober 30, 2025Yasuhiro Tsukamoto, Atsuki Kawamura, Ayhan Yurtsever, Hidefumi Suzuki, Nichole Marcela Rojas-Chaverra, Hiroki Sato, Daisuke Ino, Takehiko Ichikawa, Weilin Wei, Shojiro Haji, et al.diluted into 0.5% SDS concentration, followed by avidin pull-down using SoftLinkTM Soft Release Avidin Resin (Promega, V2011). The avidin resin was washed with 0.5% SDS RIPA buffer (150 mM NaCl, 1% Triton X-100,

(8) Soluble CSF1R promotes microglial activation and amyloid clearance in alzheimer's diseaseJ NeuroinflammationOctober 28, 2025Lianshuai Zhang, Kai Chen, Ruicheng Zeng, Guojun Bu, Xiao-Fen Chen(sTREM2-TEV-Fc and sCSF1R-TEV-Fc) were purified from culture supernatants using rProtein G Agarose Resin (YEASEN, 36405ES25). The bound proteins were eluted and incubated with TEV protease (Promega, V6101),

(9) CCDC32 collaborates with the membrane to assemble the AP-2 clathrin adaptor complexbioRxivAugust 5, 2025Dillon E. Sloan, Ariel Matthews, Haruaki Yanagisawa, Tanita Tedamrongwanish, Kevin Cannon, Jake Simmons, Garrett Chappell, Nathan I. Nicely, Rebecca Berlow, Masahide Kikkawa, et al.proteins were added. Tubes were rotated for ~1 hour at room temperature for 4 hr or overnight at 4°C. Resin was pelleted in a magnetic stand and washed with base buffer three times. 30 μL base buffer containing

(10) Targeted O-GlcNAcylation of CK2α Triggers Its Ubiquitin-Proteasome Degradation and Alters Downstream PhosphorylationACS Chemical BiologyJuly 18, 2025Tongyang Xu, Bowen Ma, Yuanpei Li, Zhihao Guo, Miaomiao Zhang, Billy Wai-Lung Ngtransfected in each dish for 48 h before collecting. Next, the HaloTag proteins were purified using Halo-Link Resin (Promega, G1912) according to the manufacturer’s protocol. To verify the purified proteins, 2× loading

(11) MicroRNA mechanisms instructing Purkinje cell specificationNeuronMay 21, 2025Norjin Zolboot, Yao Xiao, Jessica X. Du, Marwan M. Ghanem, Su Yeun Choi, Miranda J. Junn, Federico Zampa, Zeyi Huang, Ian J. MacRae, Giordano Lippiprotein was aliquoted, flash-frozen in liquid N2, and stored at −80 °C. Preparation of Halo-SpyCatcher3 Resin 400 μL of HaloLink™ resin (Promega, G1912) was washed three times with Halo binding buffer (1x PBS

(12) The Porphyromonas gingivalis RNA-binding protein is required for growth in high levels of zinc and persistence with host cellsFront Cell Infect MicrobiolMay 15, 2025Sai Yanamandra, Holly Marsh, Romana Cvitkovic, Qin Gui, Benjamin R. Belvin, Janina P. Lewisgeneration and sequencing. (ii) Halo-tagged proteins Halo-tagged proteins were pulled down using Halo-Link Resin (Promega), and 60 μg of P. gingivalis W83 RNA was added to each of the resin-tagged recombinant proteins.

(13) N6-methyladenosine in DNA promotes genome stabilityElifeApril 7, 2025Brooke A Conti, Leo Novikov, Deyan Tong, Qing Xiang, Savon Vigil, Thomas J McLellan, Chuong Nguyen, Nancy De La Cruz, Reshma T Veettil, Prashant Pradhan, et al.drug",Trypsin,Promega,Catalog # V5111, "Chemical compound, drug",Easy-Spray 50 cm column packed with 2 mm C-18 Resin,Thermo Fisher,Catalog # ES903, "Chemical compound, drug",DNA Degradase Plus,Zymo Research,Catalog #

(14) Surface microlayer-mediated virome dissemination in the Central ArcticMicrobiomeOctober 24, 2024Janina Rahlff, George Westmeijer, Julia Weissenbach, Alfred Antson, Karin Holmfeldt4 °C. DNA was isolated from 1 mL of the concentrated supernatant using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as previously described [78]. A successful cross-pole

(15) Adaptation strategies of giant viruses to low-temperature marine ecosystemsThe ISME JournalAugust 23, 2024Marianne Buscaglia, José Luis Iriarte, Frederik Schulz, Beatriz Díezultracentrifugal filters. Viral DNA was then extracted using Wizard columns (Wizard DNA Purification Resin (Promega #A7181) and Wizard Mini Columns [Promega # A7211]), and DNA was quantified using the Qubit 2.0 Fluorometer

(16) Giant viral signatures on the Greenland ice sheetMicrobiomeMay 17, 2024Laura Perini, Katie Sipes, Athanasios Zervas, Christopher Bellas, Stefanie Lutz, Mohammad Moniruzzaman, Rey Mourot, Liane G. Benning, Martyn Tranter, Alexandre M. Anesioviral particle suspensions were treated with Wizard Polymerase Chain Reaction Preps DNA Purification Resin (Promega, Fitchburg, WI, USA) at a ratio of 1-mL sample to 1-mL resin and eluted with TE buffer (10-mM

(17) Denaturing purifications demonstrate that PRC2 and other widely reported chromatin proteins do not appear to bind directly to RNA in vivoMolecular CellApril 4, 2024Jimmy K. Guo, Mario R. Blanco, Ward G. Walkup, Grant Bonesteele, Carl R. Urbinati, Abhik K. Banerjee, Amy Chow, Olivia Ettlin, Mackenzie Strehle, Parham Peyda, et al.capture, 200 μL of 25% HaloLink Resin (50 μL of HaloLink Resin total) was used per 10 million cells. Resin was washed three times with 2 mL of 1X TBS (50 mM Tris pH 7.5, 150 mM NaCl) and incubated in 1X Blocking

(18) Cleft palate and minor metabolic disturbances in a mouse global Arl15 gene knockoutThe FASEB JournalNovember 1, 2023Ying Bai, Liz Bentley, Chao Ma, Navaratnam Naveenan, James Cleak, Yixing Wu, Michelle M. Simon, Henrik Westerberg, Ramón Casero Cañas, Neil Horner, et al.50 μL of lysis buffer containing 300 ng/μL flag peptide (F3290, Sigma-Aldrich, Germany) overnight. Resin bound protein slurry was used for GTPase-Glo™ Assay (Promega, USA). For GTPase-Glo Assay, we first titrated

(19) Ecogenomics and cultivation reveal distinctive viral-bacterial communities in the surface microlayer of a Baltic Sea slickISME CommunicationsSeptember 18, 2023Janina Rahlff, Matthias Wietz, Helge-Ansgar Giebel, Oliver Bayfield, Emelie Nilsson, Kristofer Bergström, Kristopher Kieft, Karthik Anantharaman, Mariana Ribas-Ribas, Hannah D. Schweitzer, et al.metagenomes DNA from 1 ml of harvested phage stock was extracted using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as described previously [53]. Bacterial genomic DNA

(20) KIAA0317 regulates SOCS1 stability to ameliorate colonic inflammationThe FEBS journalAugust 1, 2023Lockwood KC, Lear TB, Rajbhandari S, McKelvey AC, Dunn SR, Boudreau ÁN, Liu Y, Chen BB.(Promega) reticulocyte in vitro–synthesized tagless KIAA0317 and SOCS1-V5 proteins were purified via HisPur Resin (Thermo Fisher Scientific), and reaction products were processed for V5 immunoblotting. Cell-based

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Resin

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Resin from Promega

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