InstaGene™ Matrix from Bio-Rad

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ItemInstaGene™ Matrix
CompanyBio-Rad
Price $71.00Supplier Page View Company Product Page
Catalog Number7326030

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Characterization of Antimicrobial Resistance and Potential Zoonotic Risk in Uropathogenic Escherichia coli Isolated from Companion Animals, with Genomic Analysis of Virulence Determinants in a Representative IsolateTrop Med Infect DisApril 13, 2026Asanka R. DeZoysa, Madeline Kwan, Lekshmi K. Edison, Rebecca Barber, Lisa Glick, Thomas Denagamage, Subhashinie Kariyawasam[11]. 2.3. Multiplex PCR for Urovirulence Genes Genomic DNA was extracted from the isolates using the InstaGene Matrix kit (Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s instructions. A previously

(2) Tracking persistent and resistant Enterococcus faecalis and E. faecium from farm to fork: biofilm-linked risks in antibiotic resistance of isolatesVeterinary Research CommunicationsApril 1, 2026Kursat Koskeroglu, Nurhan Ertas Onmaz, Dursun Alp Gundog, Candan Gungor, Guven Gungor, Kálmán Imre, Adriana Morarisolates by real-time PCR Genomic DNA was extracted from presumptive Enterococcus isolates using the InstaGene Matrix DNA extraction kit (Bio-Rad, USA) according to the manufacturer’s instructions. The concentration and

(3) Genome data mining of a novel Stutzerimonas marianensis strain LB-0542 isolated from pelagic Sargassum seaweed waste for plastic-degrading and plant growth-promoting traitsData in BriefApril 1, 2026Bidyut R. Mohapatra, Linel S. Moralez, Kiya E. Jameswere collected by centrifugation (12,000 g for 10 min at 4 °C) and used for the DNA extraction using InstaGene™ matrix (Bio-Rad Laboratories, Canada). The purification of the extracted DNA was performed with ethanol precipitation

(4) Safety, Immunogenicity, and Vaccine Compatibility of a Trivalent Inactivated In Ovo Nanovaccine Against Avian Colibacillosis in Broilers Under Commercial Hatchery ConditionsAnimals (Basel)March 16, 2026Angelo Scuotto, Daniela Ogonczyk-Makowska, Romain Magnez, Bryan Thiroux, Pierre-Louis Herrouin, Thomas Bouillet, Anaïs-Camille Vreulx, Amélie Degraeve, Didier Betbederserotype-specific O-antigen gene clusters. Genomic DNA was extracted from bacterial colonies using the InstaGene matrix (Bio-Rad Laboratories, Hercules, CA, USA), following the manufacturer’s protocol. Briefly, three to

(5) Genomic and phenotypic characterisation of Listeria monocytogenes strains isolated from pig feces on farm and from pork meat at retail in FrancePLoS OneMarch 2, 2026Evelyne Boscher, Benjamin Félix, Alain Rincé, Patricia Legrandois, Lucas Sajan, Christophe Soumet, Martine Denisserotypes 4b, 4d, 4e). DNA was extracted from fresh bacterial cultures grown on TSAYE plates using the InstaGene® Matrix (BioRad Laboratories, Marnes-la-Coquette France) according to the manufacturer’s instructions. Pulsed-field gel electrophoresis

(6) Characterization of Salmonella spp. Based on CRISPR PCR and FT-IR Approaches: A Pilot Study in Northern ItalyPathogensMarch 2, 2026Clara Tramuta, Irene Floris, Monica Pitti, Giulia Federica Cazzaniga, Miriam Cordovana, Daniela Manila Bianchi4). 2.2. DNA Extraction and CRISPR PCR Approach Genomic DNA from each isolate was extracted by using an Instagene matrix (Bio-Rad, Richmond, CA, USA). The CRISPR 2 primer pair (B1 and B2) used for monoplex PCR were taken

(7) Bedaquiline resistance in patients with Xpert MTB/RIF Ultra-tested rifampicin-resistant tuberculosis in the Western Cape, South Africa: a prospective studyThe Lancet MicrobeMarch 1, 2026Janré Steyn, Jennifer Williams, Fahd Naufal, Jason Limberis, Melanie Grobbelaar, Nabila Ismail, Yonas Ghebrekristos, Christoffel J Opperman, Alina Nalyvayko, Sarishna Singh, et al.extraction method followed a modified protocol,10 involving centrifugation, incubation with Bio-Rad Instagene Matrix, bead homogenisation, and purification using AMPure XP beads (Beckman Coulter, Brea, CA, USA).11 Deeplex

(8) MinION Nanopore‐Enabled Identification and Genomic Characterization of Pseudomonas syringae Complex Infecting Blueberry Using Symptomatic Plant Samples and Pathogen Pure CultureCurr ProtocFebruary 25, 2026Rishi R. Burlakoti, Sanjib Sapkota, Pragyan Burlakoti, Mark Lubberts, Amy Novinscak, Harvinder Bennypaulsuitable for downstream applications including sequencing. Materials InstaGene Matrix (Bio‐Rad, cat. no. 7326030)Pure culture of Pseudomonas spp. on solid medium (Plant Pathology laboratory at Agassiz Research and

(9) Antibacterial potential of endophytic Streptomyces spp. isolated from peanut (Arachis hypogaea) roots: bioactiveprofiling and molecular docking studiesSci RepFebruary 10, 2026Rehab M. Mohamed, Mohamed E. El Awady, Asmaa M. Fahim, Aly E. Abo-Amerphylogenetic analysis of the genomic DNA from the most antimicrobial-active isolates were conducted utilizing InstaGene Matrix (BIO-RAD) with primers listed in Table 1. The PCR reaction utilized 20 ng of genomic DNA as the template

(10) Occurrence, antibiotic susceptibility and genetic variation of Streptococcus zooepidemicus in Finnish weanling horses with and without respiratory infectionActa Veterinaria ScandinavicaJanuary 27, 2026Reija Junkkari, Anna Mykkänen, Piia Sulku, Merja Rantala, Tarja Pohjanvirta, Marjut Eklund, Sinikka Pelkonen, Thomas GrönthalIsolation of the DNA from bacterial cells was performed according to the manufacturer’s instructions using InstaGene Matrix (Bio-Rad) and the supernatant was used as a PCR template. The MLST typing was performed according to

(11) Species Identification, Virulence Factors, and Antifungal Resistance in Clinical Candida Isolates from ICU PatientsMicroorganismsJanuary 21, 2026Paola Aparecida Alves Ferreira, Lucas Daniel Cibolli Roso, Daniel Almeida Freitas, Ana Paula Pereira Bressani, Paulo Henrique da Cruz Ferreira, Emerson Cotta Bodevan, Cristiane Rocha Fagundes Moura, Rosane Freitas Schwan, Vanessa Amaral Mendonça, Karina Teixeira Magalhães-Guedes, et al.confirmation of identification. A total of 17 yeasts were selected, and their DNA was extracted using InstaGene Matrix (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions. Polymerase chain reaction (PCR

(12) Actinobacillus suis-associated iliac thromboembolism caused acute posterior paralysis in a young boarJournal of Veterinary Medical ScienceJanuary 16, 2026Masaki KONNAI, Ayaka NAMIKAWA, Haruka MAKINO, Yui HAMAZAKI, Megumi AWAMURA, Hironobu SENBA, Megumi KUSAKARI, Susumu IWAIDE, Tomoyuki SHIBAHARARNA gene (16S rDNA) sequencing. DNA was extracted from bacterial colonies using a DNA extraction kit (InstaGene Matrix; Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s instructions. A variable region

(13) Symbiont- and Bacterial Cell Size-dependent Backpacking and Grazing of AcanthamoebaMicrobes and EnvironmentsJanuary 8, 2026Nana Tanaka, Kotoka Kuriki, Torahiko Okubo, Yoshiaki Enoeda, Ayano Konno, Shinji Nakamura, Toyotaka Sato, Shin-ichi Yokota, Hiroyuki Yamaguchipresence of 6 M guanidine at 68°C for 20‍ ‍min, and DNA was then extracted using a DNA extraction kit (InstaGene Matrix; Bio-Rad). The gyrA gene was amplified from the extracted DNA using a primer set (F 5′-ACG TAC TAG GCA

(14) Regulatory RNA Ern0160 controls Enterococcus faecium virulence through direct modulation of expression of LysM domain-containing proteinsBMC GenomicsJanuary 5, 2026Loren Dejoies, Valérie Bordeau, Killian Le Neindre, Sophie Reissier, Kevin Arnould, Brice Felden, Svetlana Chabelskaya, François Guérin, Charlotte Michaux, Vincent Cattoirinstructions. Transcription templates were amplified from E. faecium Aus0004 genomic DNA (extracted with InstaGene™ Matrix, BioRad) using forward primers containing T7 promoter sequences (detailed in Supplementary Table S2).

(15) Moderate heating of waterline improves dental unit water quality by activating bactericidal properties of tap waterJournal of Dental SciencesJanuary 1, 2026Kunihiro Fushimi, Masahiro Yamada, Jun Watanabe, Jumpei Washio, Nobuhiro Takahashi, Hiroshi Egusasequencing Three colonies per group (R2A plates) were analyzed. Colonies were suspended in 6 % Chelex resin (InstaGene Matrix, Bio-Rad), and DNA was extracted via centrifugation. PCR amplification of 16S rRNA genes was performed

(16) Structural characterisation of nucleotide sugar short‐chain dehydrogenases/reductases from the thermophilic pseudomurein‐containing methanogen Methanothermobacter thermautotrophicus ΔHThe FEBS JournalJanuary 1, 2026Vincenzo Carbone, Linley R. Schofield, Patrick J. B. Edwards, Andrew J. Sutherland‐Smith, Ron S. Ronimus(Braunschweig‐Süd, Germany) and grown in BY+ media at 65 °C according to Wedlock et al. [57]. DNA was isolated using InstaGene Matrix according to the manufacturer's instructions (Bio‐Rad, Hercules, CA, USA). The Mth375 gene was amplified

(17) Colistin and Biofilm-Related Genes of Positive Escherichia coli O157:H7 in Cattle (Bos taurus) Carcasses Antibiotic Resistance Profiles, Biofilm and Molecular Characterisation of IsolatesVet Med SciDecember 29, 2025Mukaddes Barel, Kursat Koskeroglu, Fatih Doğan Koca, Harun Hizlisoy, Huseyin Burak Disli, Adalet Dishan, Burcin Izzet Satıcıoglu, Serhat Hizlisoy(Pangprasit et al. 2025). 2.7 DNA and RNA Extraction Total genomic DNA (gDNA) was obtained by using InstaGene Matrix kit (Bio‐Rad), and this process was carried out as specified in the kit protocol. Additionally, bacterial

(18) Impact of different culture media and drying treatment on the isolation of Melissococcus plutonius, the causative agent of European foulbrood, from honey bee larval specimensJournal of Veterinary Medical ScienceDecember 1, 2025Yuko KITAMURA, Yusuke KUSAJIMA, Yuka SEKIYA, Reiko KAWAGUCHI, Yuka HAYASHI, Hisako KAKINUMA, Mariko OKAMOTO, Daisuke TAKAMATSU0.2 µM of each primer, and 2 µL of template DNA. Template DNA was prepared from each isolate using InstaGene Matrix (Bio-Rad Laboratories, Inc., Hercules, CA, USA) according to the manufacturer’s instructions. Cycling

(19) Disseminated Lichtheimia ramosa infection in a Japanese Black calfMedical Mycology Case ReportsDecember 1, 2025Tetsuya Komatsu, Naomi Ito, Yuji Takamura, Kennosuke Sugie, Ami Iwata, Susumu Iwaide, Tomoyuki Shibaharacolonies were observed on potato dextrose agar. Genomic DNA was extracted from the isolate using the Instagene Matrix (Bio-Rad Laboratories, Hercules, CA, U.S.A.) according to the manufacturer's instructions. PCR assays

(20) Competition between Pseudomonas species constrains ecological diversification in polymicrobial biofilmsnpj Biofilms and MicrobiomesNovember 25, 2025Rocio Espinosa, Isabel-Sophie Kramer, Cristina I. Amador, Lorrie Maccario, Asmus K. Olesen, Vaughn S. Cooper, Henriette L. Røderdown the samples for 50 min at 10,000 × g, carefully removing the supernatant, and adding 20 µl of InstaGene Matrix (BioRad, Hercules, CA, USA). Amplicon sequencing libraries were prepared using a two-step PCR, targeting

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