Fig 1: GATA3 and CD86 expression of SATB1 KO and AAVS1 control KO Treg and Tconv cells.(A) Mean fluorescence intensity (MFI) of GATA3 expression of SATB1 KO and AAVS1 control KO Treg and Tconv cells treated with or without IL-12. n = 6–9 (biological replicates), paired t test. (B) Mean fluorescence intensity (MFI) of CD86 and FOXP3 expression of SATB1 KO and AAVS1 control KO Treg and Tconv cells treated with or without IL-12. n = 8–9 (biological replicates), paired t test. (C, D) AAVS1 and SATB1 KO Tconv cell expansion rate at day 14, 15, 16, and 18 of culture with or without the addition of IL-12. (C) Absolute cell counts with mean and SD, (D) cell counts of SATB1 KO Tconv cells normalized to respective AAVS1 KO control cells. n = 6 (biological replicates). (E, F) Extracellular IL-6 levels determined by LEGENDplexTM assay of IL-12-treated AAVS1 KO and SATB1 KO Treg (E) or Tconv cells (F). Absolute values (left), normalized to AAVS1 KO controls (right), n = 7–10 (biological replicates), paired t test.