Latex Beads-Rabbit IgG-FITC Complex from Cayman Chemical

Product Specs
ItemLatex Beads-Rabbit IgG-FITC Complex
CompanyCayman Chemical
Price $321.00Supplier Page View Company Product Page
Catalog Number400291
Quantity1 ea
Format150 µl of latex beads coated with rabbit-IgG conjugated to fluorescein
Storage4
OriginUSA
Source/Expression SystemAnimal/Rabbit

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Microglial Fkbp5 Impairs Post‐Stroke Vascular Integrity and Regeneration by Promoting Yap1‐Mediated Glycolysis and Oxidative PhosphorylationAdvanced ScienceMarch 1, 2026Yanan Li, Yanmei Qiu, Yunlei Yang, Yanhao Wei, Haokun Peng, Longhai Zeng, Pengcheng Li, Rentang Bi, Bo Huusing Phagocytosis Assay Kit (IgG FITC) (500290, Cayman, USA). Briefly, BV2 cells were incubated with latex beads‐rabbit IgG‐FITC complex at 37 °C for 1 h in the dark after treatment. The cells were incubated with trypan blue quenching solution

(2) Mesenchymal stem cell-derived exosome delivery of let-7a-5p enhances macrophage efferocytosis via Arid3a/Mertk axis in acute-on-chronic liver failureStem Cells Transl MedDecember 2, 2025Junyi Wang, Zhihui Li, Zhouhan Wang, Wei Liang, Shibo Meng, Junfeng Chen, Jialei Wang, Jing Zhang, Bingliang Linphagocytosis assay The assay was conducted using a phagocytosis kit (Cayman, MI, USA). After adding the Latex Beads-Rabbit IgG-FITC complex to the culture medium (1:400), the mixture was incubated at 37 °C and 5% CO2 for 2 h. Hoechst33342 (Beyotime)

(3) Hyaluronan provokes inflammation but suppresses phagocytotic function in macrophagesJournal of Molecular and Cellular CardiologyJanuary 1, 2025Timothy N. Audam, Caitlin M. Howard, Danielle T. Little, Lauren F. Garrett, Yi Wei Zheng, Zhen Gu, Kenneth R. Brittian, Raéden Gray, Julia Chariker, Richa A. Singhal, et al.ng/ml IL-4 and 10 ng/ml IL-13. Macrophage phagocytosis: We performed macrophage phagocytosis using latex beads-rabbit IgG-FITC complex kit (Cayman Chemical, #500290), following a standard protocol for measuring phagocytosis in adherent

(4) Multi-omic analysis of Huntington's disease reveals a compensatory astrocyte stateNature communicationsAugust 8, 2024Paryani F, Kwon JS, Ng CW, Jakubiak K, Madden N, Ofori K, Tang A, Lu H, Xia S, Li J, et al.the latter, the media was changed into fresh HMC3 media containing fluorescent latex beads (1:500, latex –beads-rabbit IgG-FITC complex—Cayman chemical cat#500290). The cells were incubated for 30 min at 37 °C before being washed as the

(5) Osteopontin drives neuroinflammation and cell loss in MAPT-N279K frontotemporal dementia patient neuronsCell Stem CellMay 2, 2024Osama Al-Dalahmah, Matti Lam, Julie J. McInvale, Wenhui Qu, Trang Nguyen, Jeong-Yeon Mun, Sam Kwon, Nkechime Ifediora, Aayushi Mahajan, Nelson Humala, et al.DeepRed,ThermoFisher,Cat#C10422 Fluorescent latex beads (latex beads-rabbit IgG-FITC complex),Cayman chemical,Cat#400291 Seahorse XFp Extracellular Flux Analyzers,Agilent,Cat#102340–001 RNAscope™ multiplex fluorescent v2,ACDbio,Cat#323110 Predesigned

(6) Disulfiram Reduces Atherosclerosis and Enhances Efferocytosis, Autophagy, and Atheroprotective Gut Microbiota in Hyperlipidemic MiceJournal of the American Heart AssociationApril 16, 2024C. Alicia Traughber, Kara Timinski, Ashutosh Prince, Nilam Bhandari, Kalash Neupane, Mariam R. Khan, Esther Opoku, Emmanuel Opoku, Gregory Brubaker, Junchul Shin, et al.by imaging by confocal microscopy. For phagocytosis assay, the THP‐1 macrophages were incubated with Latex beads‐rabbit‐IgG‐FITC complex (Cayman Chemical # 500290) with a final dilution of 1:200 at 37 °C for 1 hour. Cells were washed with

(7) Functional characterization of Alzheimer's disease genetic variants in microgliaNature geneticsOctober 1, 2023Yang X, Wen J, Yang H, Jones IR, Zhu X, Liu W, Li B, Clelland CD, Luo W, Wong MY, et al.Supplementary Table 8. Microglia phagocytosis assay We incubated microglia-like cells with diluted latex beads (rabbit IgG-FITC complex) (Cayman 500290) at 37 °C for 2 hours. Trypan blue quenching solution was added for 2 min, followed

(8) A simple protocol for isolating microglia from adult mouse brainNeuroImmune Pharmacology and TherapeuticsSeptember 26, 2023Sudipta Chakrabarti, Sukhamoy Gorai, Kalipada Pahanisolated from WT adult mice were plated on poly-D-lysine-coated glass coverslips. Cells were treated with latex beads-rabbit IgG-FITC complex (Cayman Chemical, Ann Arbor, MI) at a dilution of 1:200 for 30 min. For LPS-stimulated microglia, cells

(9) Reprogramming of PD-1+ M2-like tumor-associated macrophages with anti–PD-L1 and lenalidomide in cutaneous T cell lymphomaJCI InsightJuly 10, 2023Zhen Han, Xiwei Wu, Hanjun Qin, Yate-Ching Yuan, Daniel Schmolze, Chingyu Su, Jasmine Zain, Lilach Moyal, Emmilia Hodak, James F. Sanchez, et al.manufacturer’s instructions. Briefly, latex beads coated with rabbit IgG-FITC complex (Cayman Chemical, catalog 400291) were added directly to M2-like TAMs that were induced with CTCL cell line supernatant to a final dilution

(10) Macrophage polarization is involved in liver fibrosis induced by β (1)-adrenoceptor autoantibodyActa biochimica et biophysica SinicaAugust 25, 2022Wu Y, Fan X, Yu H, Liu J, Duan Y, Zhang S, Yan L, Du Y, Liu H.www.raybiotech.com). Phagocytosis assay RAW264.7 cells were plated on a 4-well chamber slide and allowed to adhere overnight. The latex beads-Rabbit IgG-FITC complex (Cayman Chemical, Ann Arbor, USA) was added directly to the culture medium (1:200) and incubated at

(11) Musashi-1 Regulates MIF1-Mediated M2 Macrophage Polarization in Promoting Glioblastoma ProgressionCancersApril 9, 2021Yang YP, Chien CS, Yarmishyn AA, Chan MS, Lee AC, Chen YW, Huang PI, Ma HI, Lo WL, Chien Y, et al.polarized into M1/M2 by standard protocols or incubated with rMIF or a conditioned medium. After 48 h, a Latex Beads-Rabbit IgG–FITC complex (Cayman Chemical, Ann Arbor, MI, USA) was added for 24 h. To prepare a Latex Beads-Rabbit IgG–FITC solution,

(12) PDLIM2 repression by ROS in alveolar macrophages promotes lung tumorigenesisJCI InsightMarch 8, 2021Liwen Li, Fan Sun, Lei Han, Xujie Liu, Yadong Xiao, Alyssa D. Gregory, Steven D. Shapiro, Gutian Xiao, Zhaoxia Quindicated mice were seeded in an ultralow attachment plate (Corning Inc.) for 20 minutes, and then Latex Beads-Rabbit IgG-FITC Complex (Cayman Chemical, 1:100) was directly added and cultured for 2 hours. The phagocytic abilities of AMs

(13) Extracts of Phellinus linteus, Bamboo (Sasa senanensis) Leaf and Chaga Mushroom (Inonotus obliquus) Exhibit Antitumor Activity through Activating Innate ImmunityNutrientsJuly 29, 2020Jun Fang, Shanghui Gao, Rayhanul Islam, Yuji Teramoto, Hiroshi MaedaMaterials Dextran Sulfate Sodium Salt (DSS) was purchased from MP Biomedicals, LLC (Irvine, CA, USA). Latex beads-Rabbit IgG-FITC Complex was from Cayman chemical (Ann Arbor, MI, USA). Thiazolyl Blue Tetrazolium Bromide (MTT) was purchased

(14) Ethanol Exposure Impairs AMPK Signaling and Phagocytosis in Human Alveolar Macrophages: Role of Ethanol MetabolismAlcoholism, clinical and experimental researchAugust 1, 2019Kaphalia L, Srinivasan MP, Kakumanu RD, Kaphalia BS, Calhoun WJ.AM To evaluate the phagocytosis, freshly isolated AM were incubated with EtOH (4 mg/ml) along with latex Beads-Rabbit IgG-FITC complex [Cayman’s Phagocytosis Kit (IgG-FITC) coated with fluorescent-labeled rabbit-IgG] at 37°C for 24 hr

(15) High fat diet worsens the impact of ageing on microglial function and morphology in a region-specific mannerNeurobiology of AgingFebruary 1, 2019Sarah J. Spencer, Bashirah Basri, Luba Sominsky, Alita Soch, Monica T. Ayala, Philipp Reineck, Brant C. Gibson, Ruth M. BarrientosLab-Tek II; ThermoFisher Waltham, MA, USA). The microglia were incubated at 37 °C, 5% CO3 for 2 hr. Latex beads-rabbit IgG-FITC complex (1:100 final concentration on cells; Cayman Chemicals, Ann Arbor, MI, USA) were then added to the wells.

(16) Regulation of Macrophage Activation and Polarization by HCC-Derived Exosomal lncRNA TUC339International journal of molecular sciencesSeptember 28, 2018Li X, Lei Y, Wu M, Li N.Assay THP-1 cells (1 × 106 /well) were plated in six-well culture plates and allowed to adhere overnight. The Latex beads-rabbit IgG-FITC complex (Cayman, Ann Arbor, MI, USA) was added directly to the culture medium at a 1:200 dilution and incubated

(17) Neuroinflammatory priming to stress is differentially regulated in male and female ratsBrain, Behavior, and ImmunityMay 1, 2018Laura K. Fonken, Matthew G. Frank, Andrew D. Gaudet, Heather M. D'Angelo, Rachel A. Daut, Emma C. Hampson, Monica T. Ayala, Linda R. Watkins, Steven F. Maierincubated at 37⁰C, 5% CO2 for 2 h and then latex beads-rabbit IgG-FITC complex (at 1:100; Cayman Chemicals, 400291) were added to the wells. After a 30 min incubation, the beads were washed off with PBS and the cells

(18) MIF-CD74 signaling impedes microglial M1 polarization and facilitates brain tumorigenesisOncogeneDecember 1, 2016A Ghoochani, M A Schwarz, E Yakubov, T Engelhorn, A Doerfler, M Buchfelder, R Bucala, N E Savaskan, I Y Eyüpoglutreated with 100 ng/ml rMIF or PBS as control. Latex Beads-Rabbit IgG-FITC complex (Cayman, cat. no. 400291) was added for 24 h. To prepare a Latex Beads-Rabbit IgG-FITC solution, beads solution were diluted

(19) AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mitesProceedings of the National Academy of Sciences of the United States of AmericaJune 2, 2015Kim LK, Morita R, Kobayashi Y, Eisenbarth SC, Lee CG, Elias J, Eynon EE, Flavell RA.cells were treated with FITC-labeled chitin or FITC-conjugated latex beads (Cayman Chemical, catalog # 400291) with or without bisdionin F (Calbiochem, catalog # 112252), and then flow cytometry was performed. Limulus Test

(20) Characterization of rapid neutrophil extracellular trap formation and its cooperation with phagocytosis in human neutrophilsDiscoveries (Craiova)June 30, 2014Mona Saffarzadeh, Hector A. Cabrera-Fuentes, Florian Veit, Dongsheng Jiang, Karin Scharffetter-Kochanek, Christian Gille Gille, Suzan H. M. Rooijakkers, Dominik Hartl, Klaus T. Preissnerresuspended in phenol red-free RPMI 1640. Phagocytosis assay Phagocytosis assay was performed using latex beads-rabbit IgG-FITC complex (Cayman Chemical, Germany) or fluorescent-labeled C3b-coated beads. The latex beads had a 0.1 µm mean

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Latex Beads-Rabbit IgG-FITC Complex from Cayman Chemical

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Cayman Chemical

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Latex Beads-Rabbit IgG-FITC Complex from Cayman Chemical

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