Mini Bio-Spin® Chromatography Columns from Bio-Rad

Product Specs
ItemMini Bio-Spin® Chromatography Columns
CompanyBio-Rad
Price $207.00Supplier Page View Company Product Page
Catalog Number7326207
QuantityPkg of 100
TypeColumns

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Inhibitors supercharge kinase turnover through native proteolytic circuitsNatureJanuary 22, 2026Natalie S. Scholes, Martino Bertoni, Arnau Comajuncosa-Creus, Katharina Kladnik, Xuefei Guo, Fabian Frommelt, Matthias Hinterndorfer, Hlib Razumkov, Polina Prokofeva, Martin P. Schwalm, et al.the dark. Finally, the samples were washed twice using 1× pre-washed Mini BioSpin columns (Bio-Rad, 7326207) with wash buffer 1 (0.2% SDS in 1× PBS), followed by 16 washes with wash buffer 2 (8 M urea in 1× PBS)

(2) Therapeutic 6-thio-deoxyguanosine inhibits telomere elongation in cancer cells by inducing a non-productive stalled telomerase complexNat CommunDecember 7, 2025Samantha L. Sanford, Mareike Badstübner, Michael Gerber, William Mannherz, Noah Lampl, Rachel Dannenberg, Angela Hinchie, Matthew A. Schaich, Sua Myong, Mark Hedglin, et al.150 mM KCl. The bead slurry was nutated for 30 min at 4 °C. The eluted telomerase was collected using Mini Bio-Spin® Chromatography columns (Bio-Rad). Samples were flash-frozen and stored at − 80 °C. Dot blot quantification of telomerase concentration The

(3) A new paradigm for outer membrane protein biogenesis in the BacteroidotaNatureNovember 13, 2025Xiaolong Liu, Luis Orenday Tapia, Justin C. Deme, Susan M. Lea, Ben C. Berksrotated slowly at 4 °C for 2 h and then transferred into Mini Bio-Spin Chromatography columns (Bio-Rad, 7326207), and centrifugation at 100g for 1 min. The beads were washed 3 times with 250 µl buffer WD and bound

(4) Using BONCAT to dissect the proteome of S. aureus persistersmSphereSeptember 30, 2025Eva D. C. George Matlalcuatzi, Thomas Bakkum, Pooja S. Thomas, Stephan Hacker, Bogdan I. Florea, Bastienne Vriesendorp, Daniel E. Rozen, Sander I. van Kasterensample, formic acid (3 µL) was added, followed by filtering off the beads over biospin columns (Bio-Rad, 7326207) on top of 2 mL Eppendorf tubes using centrifugation (2 min, 300 rcf). Note: Now, the 2 mL Eppendorf

(5) RHOA-dependent regulation of mitochondrial remodeling and cell motility in hypoxia-exposed gastric epithelial cellsJournal of Cell ScienceJuly 15, 2025Aranya Pal, Prabin Bawali, Abhisek Brahma, Smruti Ranjan Rana, Rakesh Mohapatra, Debashish Chakraborty, Indrajit Poirah, Supriya Samal, Smaran Banerjee, Duane T. Smoot, et al.prepared using RIPA buffer (HiMedia, India, #TCL131). Mini bio-spin chromatography columns (Bio-rad, USA, #7326207) and glutathione Sepharose 4B GST-tagged protein purification resin (Cytiva, USA, #17075601) were used.

(6) A general and accessible approach to enrichment and characterisation of natural anti-Neu5Gc antibodies from human samples†RSC Chemical BiologyJuly 2, 2025Esme Hutton, Yumiko Uno, Emma Scott, Craig Robson, Martin A. Fascione, Nathalie Signoretwere neutralised with 0.1 M ethanolamine-HCl for 1 h. Into each empty mini Bio-spin column (BioRad, #7326207) 1 mL resin was loaded to form Neu5Gc-negative (HS or WT-HEK lysate) columns and Neu5Gc-positive (CS

(7) Challenges and strategies in the soluble expression of CTA1-(S14P5)4-DD and CTA1-(S21P2)4-DD fusion proteins as candidates for COVID-19 intranasal vaccinesPLOS ONEDecember 26, 2024Simson Tarigan, Gita Sekarmila, Apas, Sumarningsih, Ronald Tarigan, Riyandini Putri, Damai Ria Setyawatix g for 15 minutes, with the supernatant carefully collected. For small-scale purification, we used Mini-Bio Spin™ chromatography columns (Bio-Rad) with 100 μL Ni-NTA agarose (Qiagen GmbH) and a 0.5 mL sample volume. For larger-scale purification,

(8) Multi-scale photocatalytic proximity labeling reveals cell surface neighbors on and between cellsScienceJuly 19, 2024Zhi Lin, Kaitlin Schaefer, Irene Lui, Zi Yao, Andrea Fossati, Danielle L. Swaney, Ajikarunia Palar, Andrej Sali, James A. Wells(Promega, V5111), sequencing-grade chymotrypsin (Promega, V1061) and mini Bio-Spin columns (Bio-Rad, 7326207) were purchased. When performing solvent exchange processes, 7 kDa Zeba Spin desalting columns (Thermo

(9) A persistent variant telomere sequence in a human pedigreeNature CommunicationsJune 1, 2024Angela M. Hinchie, Samantha L. Sanford, Kelly E. Loughridge, Rachel M. Sutton, Anishka H. Parikh, Agustin A. Gil Silva, Daniel I. Sullivan, Pattra Chun-On, Matthew R. Morrell, John F. McDyer, et al.150 mM KCl. The bead slurry was nutated for 30 min at 4 °C. The eluted telomerase was collected using Mini Bio-Spin® Chromatography columns (Bio-Rad). Samples were flash-frozen and stored a −80 °C. End-labeling of DNA primers 50 pmol of PAGE

(10) Structure of the human 20S U5 snRNPNature structural & molecular biologyMay 1, 2024Schneider S, Brandina I, Peter D, Lagad S, Fraudeau A, Portell-Montserrat J, Tholen J, Zhao J, Galej WP.NEs to the final volume of 10% and incubated ON at 8 °C, with shaking. TMG-beads were collected in Mini Bio-Spin chromatography columns, Bio-Rad, by centrifugation for 30 s at 2,500g at 8 °C. After two wash steps with the IP buffer, beads

(11) Antibodies elicited in humans upon chimeric hemagglutinin-based influenza virus vaccination confer FcγR-dependent protection in vivoProceedings of the National Academy of SciencesOctober 31, 2023Julia E. Edgar, Stephanie Trezise, Robert M. Anthony, Florian Krammer, Peter Palese, Jeffrey V. Ravetch, Stylianos BournazosThe mixture was rotated at 4 °C for 90 min. The protein G beads were washed 5 times with PBS using Mini Bio-Spin chromatography columns (Bio-Rad) and then eluted with IgG elution buffer (Pierce) into 10% volume 1M Tris-HCl (pH 9). Polyclonal

(12) Paralog-dependent isogenic cell assay cascade generates highly selective SLC16A3 inhibitorsCell chemical biologyAugust 17, 2023Dvorak V, Casiraghi A, Colas C, Koren A, Tomek T, Offensperger F, Rukavina A, Tin G, Hahn E, Dobner S, et al.corresponded to 40 μl of slurry). Next, samples were loaded to equilibrated Biospin columns (BioRad, 7326207) washed once with buffer containing HEPES (50 mM, pH 8.0), NaCl (150 mM), NP-40 (0.5% v/v), PMSF (1

(13) Method for Identifying Galectin Ligands on Lymphocyte Membrane GlycoproteinsMethods in Molecular BiologyApril 27, 2023Kashyap R. Patel, Adam W. Barb, Sean R. Stowellfresh before each immunoprecipitation). Neutralization buffer: 1 M ammonium carbonate, pH 8.0. Mini Bio-Spin Chromatography Columns (Bio-Rad). 1.5 ml tubes and suitable refrigerated centrifuge. Bath sonicator (VWR). Tube rotator

(14) Mechanisms of a Mycobacterium tuberculosis Active PeptidePharmaceuticsFebruary 6, 2023Komal Umashankar Rao, Ping Li, Charlotte Welinder, Erik Tenland, Pontus Gourdon, Erik Sturegård, James C. S. Ho, Gabriela GodalySample Preparation for LC–MS/MS Samples (50 µL) were added to Mini Bio-Spin Chromatography Columns (#7326207, BioRad, Solna, Sweden) for the removal of the remaining resins from the Co-IP and spun 250× g for 2

(15) Manganese-driven CoQ deficiencyNature CommunicationsOctober 13, 2022Jutta Diessl, Jens Berndtsson, Filomena Broeskamp, Lukas Habernig, Verena Kohler, Carmela Vazquez-Calvo, Arpita Nandy, Carlotta Peselj, Sofia Drobysheva, Ludovic Pelosi, et al.Sigma) for 16 h (37 °C). The digested samples were separated from the beads with spin-columns (BioRad, #7326207), the volume was determined and 5 µl of gallium standard (1 mg/l) were added. The complete volume was

(16) Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIVResearch SquareSeptember 1, 2022Aljawharah Alrubayyi et al.avoid off-target neutralization due to the HIV pseudovirus backbone, we purified IgG from serum using Mini Bio-Spin Chromatography Columns (BioRad) by incubating sera with a resin of protein G Sepharose beads (GE Healthcare) for 2h, eluting

(17) Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIVbioRxivSeptember 1, 2022Aljawharah Alrubayyi et al.avoid off-target neutralization due to the HIV pseudovirus backbone, we purified IgG from serum using Mini Bio-Spin Chromatography Columns (BioRad) by incubating sera with a resin of protein G Sepharose beads (GE Healthcare) for 2h, eluting

(18) The landscape of translational stall sites in bacteria revealed by monosome and disome profilingRNAMarch 1, 2022Tomoya Fujita, Takeshi Yokoyama, Mikako Shirouzu, Hideki Taguchi, Takuhiro Ito, Shintaro IwasakiTALON Metal Affinity Resin (Clontech) was equilibrated with resuspension buffer and transferred to Mini Bio-Spin Chromatography Columns (Bio-Rad). The resuspended RNC was incubated with the resin within the column for 20 min at 4°C. The

(19) Large-scale movement of eIF3 domains during translation initiation modulate start codon selectionNucleic Acids ResearchNovember 18, 2021Jose L Llácer, Tanweer Hussain, Jinsheng Dong, Laura Villamayor, Yuliya Gordiyenko, Alan G Hinnebuschinteraction assays Pull-down assays were carried out using Glutathione agarose resins (ABT) and the Mini Bio-Spin Chromatography columns (BioRad). In each case, 50 μl of resin was pre-equilibrated with 500 μl of storage buffer and then 100

(20) Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIVNature communicationsOctober 5, 2021Alrubayyi A, Gea-Mallorquí E, Touizer E, Hameiri-Bowen D, Kopycinski J, Charlton B, Fisher-Pearson N, Muir L, Rosa A, Roustan C, et al.avoid off-target neutralization due to the HIV pseudovirus backbone, we purified IgG from serum using Mini Bio-Spin Chromatography Columns (BioRad) by incubating sera with a resin of protein G Sepharose beads (GE Healthcare) for 2 h, eluting

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