Bio-Spin® Chromatography Columns from Bio-Rad

Product Specs
ItemBio-Spin® Chromatography Columns
CompanyBio-Rad
Price $191.00Supplier Page View Company Product Page
Catalog Number7326008
QuantityPkg of 100
TypeColumns

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Lysosomal phosphoinositide turnover acts upstream of RagGTPase-mTORC1 and controls muscle growthNat MetabMarch 18, 2026Melanie Picot, Nesrine Hifdi, Mathilde Vaucourt, Melanie Mansat, Peng Li, Isabel Singer, Gaëtan Chicanne, Alexandre Stella, Shen Kuang, Caterina Miceli, et al.Ni-NTA-beads equilibrated in lysis buffer. After incubation, the beads were loaded onto a biospin column (7326008 Bio-Rad). The column was subsequently washed with eight column volumes of lysis buffer containing 25

(2) HLA ‐ DRB1 Allelic Combinations Differentially Shape Dendritic Cell Antigen Presentation Enhanced by Tumour Cell Line Lysate‐PulsingHLAFebruary 1, 2026Gonzalo Lázaro, Juan A. Cedano, Maitane Faus, Carme Roura‐Mir, Laia Garrigós, José Pérez‐García, Javier Román‐García, Javier Cortés, Andrea Aran, Mercè Martílysis buffer. After incubation, HLA‐DR‐peptide complexes were purified by immunoprecipitation using Bio‐Spin Chromatography Columns (Bio‐Rad). Non‐retained fractions were collected and stored for future analysis. Retained fractions

(3) A single vesicle fluorescence microscopy platform to quantify phospholipid scramblingbioRxivDecember 1, 2025Sarina Veit, Grace I. Dearden, Kartikeya M. Menon, Faria Noor, Indu Menon, Takefumi Morizumi, Oliver P. Ernst, Anant K. Menon, Thomas Gúnther Pomorskifrom Avanti Polar Lipids Inc. (Birmingham, AL, USA). Bio-Gel P-6 (#1504130) and Bio-Spin columns (#7326008) were purchased from Bio-Rad (Hercules, CA, USA). Alexa Fluor™ 647 C2 Maleimide (#A20347), ethylene

(4) Age and origin of a Cahokian wooden monument at the Mitchell site, Illinois, USAPLoS OneOctober 3, 2025Nicholas V. Kessler, Erin M. Benson, Timothy R. Pauketat, Jason D. Kirk, Matthew D. Therrellpolypropylene disposable columns (Bio-Spin® Chromatography Columns, Bio-Rad, Hercules, California, part number 7326008) using Sr resin (Sr-Spec Resin®) and twice-distilled acids [76]. Purified Sr was loaded unto Tantalum

(5) Roles of protein–protein interactions and monolayer mechanics in tricellulin localization to tricellular tight junctionsBiology OpenSeptember 15, 2025Toiba Mushtaq, Jaakko Lehtimäki, Konstantin Kogan, Johan Peränen, Xionan Liu, Markku Varjosalo, Aki Manninen, Pekka LappalainenLysate was centrifuged twice at 16,000 g at 4°C to remove the insoluble material. During centrifugation, Bio-Spin chromatography columns (Bio-Rad Laboratories) were loaded with Strep-Tactin Sepharose beads (400 µl 50% slurry; IBA Lifesciences)

(6) A TOM1 variant impairs interaction with TOLLIP, autophagosome-lysosome fusion and regulation of innate immunityDisease models & mechanismsSeptember 1, 2025Lång HKM, Roach TG, Hölttä M, Keskitalo S, Varjosalo M, Heiskanen K, Collins MV, Seppänen MRJ, Capelluto DGS, Ikonen E, et al.Benzonase Nuclease (Santa Cruz Biotechnology) on ice. Proteins were bound to Strep-Tactin sepharose and Bio-Spin chromatography columns (Bio-Rad) and eluted with D-biotin (Thermo Fisher Scientific). After C18 purification, samples were

(7) Akkermansia muciniphila‐Derived N‐Acetylspermidine Modulates the Localization of Intestinal α1,2‐Fucosylated Proteins to Maintain Gut HomeostasisAdvanced ScienceAugust 7, 2025Ye Yao, Zhangming Pei, Yuanyuan Dai, Yinghan Chen, Zepeng Chang, Hongchao Wang, Jianxin Zhao, Hao Zhang, Qixiao Zhai, Wenwei Lu, et al.Agarose‐bound Ulex Europaeus Agglutinin I (UEA I), (AL‐1063‐2, Vector laboratories, USA) was drawn into the Bio‐Spin Chromatography Columns (732‐6008, Bio‐Rad, USA) and washed with 10 column volumes of HBS buffer (10 mm HEPES, 0.15 m NaCl,

(8) Protocol for measuring β-arrestin1-phosphorylated peptide interaction and analyzing conformational dynamics of β-arrestin1STAR ProtocMay 9, 2025Kiae Kim, Ka Young Chungliquid chromatography),Cytiva,N/A NGC chromatography system,Bio-Rad,N/A Epoch microplate reader,BioTek,N/A Bio-Spin chromatography columns,Bio-Rad,Cat# BR7326008 Xevo G2-XS QTof,Waters,N/A Immobilized pepsin column (2.1 × 30 mm),Life Technologies,Cat#

(9) Amlodipine, an L-type Ca2+ channel inhibitor, regulates release of extracellular vesicles from tumor cellsCarcinogenesisApril 3, 2025Sujan K Mondal, Chang-Sook Hong, Jie Han, Brenda Diergaarde, Dan P Zandberg, Theresa L Whitesidefilter), and 100 µl of filtered plasma was applied to a small Bio-spin Chromatography Column (Bio-Rad #7326008) packed with Sepharose 2B for sEV isolation by SEC. PBS was used for elution, and 200 µl fractions were

(10) Adeno-associated viral delivery of Env-specific antibodies prevents SIV rebound in macaques after discontinuing antiretroviral therapyScience ImmunologyFebruary 28, 2025Vadim A. Klenchin, Natasha M. Clark, Nida K. Keles, Saverio Capuano, Rosemarie Mason, Guangping Gao, Aimee Broman, Emek Kose, Taina T. Immonen, Christine M. Fennessey, et al.pellets were mixed with 1 ml WB, transferred to 1 ml columns with pre-wetted bottom frit (Bio-Rad, cat. 7326008), and further washed three times with 1 ml WB by gravity flow. The columns were then inserted into 5

(11) Diet-derived urolithin A is produced by a dehydroxylase encoded by human gut Enterocloster speciesNat CommunJanuary 24, 2025Reilly Pidgeon, Sacha Mitchell, Michael Shamash, Layan Suleiman, Lharbi Dridi, Corinne F. Maurice, Bastien Castagnerfreshly prepared by loading 1 mL of nickel-nitrilotriacetic acid (Ni-NTA) agarose (QIAGEN) slurry into Bio-Spin chromatography columns (Bio-Rad). The Ni-NTA agarose bed (0.5 mL) was then washed with 5 mL wash buffer (1X PBS, 1 mM DTT,

(12) ALBA proteins facilitate cytoplasmic YTHDF-mediated reading of m6A in ArabidopsisThe EMBO JournalNovember 29, 2024Marlene Reichel, Mathias Due Tankmar, Sarah Rennie, Laura Arribas-Hernández, Martin Lewinski, Tino Köster, Naiqi Wang, Anthony A Millar, Dorothee Staiger, Peter Brodersenammonium,Sigma,45520 Ampicillin,Serva,13398.02 Kanamycin,Serva,26898.03 IPTG,Thermo Fisher,R0392 Ni-NTA agarose beads,macherey-nagel,745400.25 Gravity column,Bio-Rad,7326008 Imidazole,Thermo Scientific,J17525 His6-tagged ULP1 protease,"Kind gift from Prof. Birthe Kragelund,

(13) Proviral role of ATG2 autophagy related protein in tomato bushy stunt virus replication through bulk phospholipid transfer into the viral replication organelleMolecular Biology of the CellOctober 1, 2024Yuanrong Kang, Judit Pogany, Peter D. Nagyinhibitor cocktail). The supernatants were incubated with anti-Flag M2 affinity agarose (Sigma-Aldrich) in Bio-spin chromatography columns (Bio-rad) for 2 h at 4°C on a rotator, followed by washing with the washing buffer (10% [vol/vol] glycerol,

(14) Multiparametric profiling of HER2-enriched extracellular vesicles in breast cancer using Single Extracellular VEsicle NanoscopyJournal of NanobiotechnologySeptember 28, 2024Nan Jiang, Andras Saftics, Eugenia Romano, Ima Ghaeli, Cristal Resto, Vanessa Robles, Saumya Das, Kendall Van Keuren-Jensen, Victoria L. Seewaldt, Tijana Jovanovic-Talisman400 µL and mixed. The diluted Capto Core resin solution was then transferred to spin column (BIO-RAD, 7326008) and centrifuged at 500×g for 3 min to remove the storage buffer. After two washes with 500 µL of PBS,

(15) Adeno-associated viral delivery of Env-specific antibodies prevents SIV rebound after discontinuing antiretroviral therapybioRxivJune 7, 2024Vadim A. Klenchin, Natasha M. Clark, Nida K. Keles, Saverio Capuano, Rosemarie Mason, Guangping Gao, Aimee Broman, Emek Kose, Taina T. Immonen, Christine M. Fennessey, et al.pellets were mixed with 1 ml WB, transferred to 1 ml columns with pre-wetted bottom frit (Bio-Rad, cat. 7326008) and further washed three times with 1 ml WB by gravity flow. The columns were then inserted into 5

(16) Activity-Based Protein Profiling to Probe Relationships between Cytochrome P450 Enzymes and Early-Age Metabolism of Two Polycyclic Aromatic Hydrocarbons (PAHs): Phenanthrene and ReteneChemical Research in ToxicologyMay 20, 2024Kari A. Gaither, Whitney L. Garcia, Kimberly J. Tyrrell, Aaron T. Wright, Jordan N. Smith(Waltham, Massachusetts, USA). Sequencing grade trypsin was obtained from Promega, (Madison, WI, USA), and Bio-Spin chromatography columns from Bio-Rad (Hercules, CA, USA). N-(3-azidopropyl)biotinamide (biotin-azide) was purchased from TCI

(17) Quantification of Adaptive Immune Responses Against Protein-Binding Interfaces in the Streptococcal M1 ProteinMolecular & Cellular ProteomicsMay 1, 2024Eva Torres-Sangiao, Lotta Happonen, Morizt Heusel, Frida Palm, Carlos Gueto-Tettay, Lars Malmström, Onna Shannon, Johan Malmströmdone essentially as described (39). Briefly, Strep-Tactin Sepharose beads (IBA) were packaged in a Bio-Spin Chromatography Columns (BioRad) and equilibrated in PBS buffer, pH 7.4, for afterwards charged with 10 μg of recombinant TAP-tagged

(18) Subversion of selective autophagy for the biogenesis of tombusvirus replication organelles inhibits autophagyPLoS PathogMarch 14, 2024Yuanrong Kang, Wenwu Lin, Peter D. Nagyinhibitor cocktail). The supernatants were incubated with anti-Flag M2 affinity agarose (Sigma-Aldrich) in Bio-spin chromatography columns (Bio-rad) for 2 h at 4°C on a rotator, followed by washing with the washing buffer (10% [v/v] glycerol,

(19) A rapid, tag-free way to purify functional GPCRsJournal of Biological ChemistryJanuary 1, 2024Anthony D. Shumate, David L. Farrensslurry in storage buffer) to a clean 1.5 ml polypropylene chromatography column (Bio-Rad; catalog no.: 7326008) and pre-equilibrated with 5 CV of cold binding buffer. Binding of rhodopsin to the resin was initiated

(20) A Single-Step Immunocapture Assay to Quantify HCC Exosomes Using the Highly Sensitive Fluorescence Nanoparticle-Tracking AnalysisJournal of Hepatocellular CarcinomaNovember 2, 2023Ali Riza Koksal, Nergiz Ekmen, Yucel Aydin, Kelley Nunez, Tyler Sandow, Molly Delk, Martin Moehlen, Paul Thevenot, Ari Cohen, Srikanta DashSephadex G-200 (“hand-made gel column”, A 50120, Sigma, Burlington MA), Bio-Spin Chromatography Columns (7326008 Bio-Rad, Hercules, CA), Cell guidance Exo-spin mini columns (EX03-50), and Amersham MicroSpin G-50 Columns

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