Affi-Prep Protein A Support from Bio-Rad

Product Specs
ItemAffi-Prep Protein A Support
CompanyBio-Rad
Price $2,232.00Supplier Page View Company Product Page
Catalog Number1560005

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) STAG2-truncating variants reveal a mosaic STAG2-inactivation pattern and compensatory mechanisms involving cohesin complex remodelingiScienceNovember 22, 2025Macarena Moronta-Gines, Marja W. Wessels, Valentina Casa, Thomas van Staveren, Amber Hof, Wendy K. Chung, Marjolaine Willems, Anna Sandestig, Irina Huening, Peter Turnpenny, et al.(20 mM Tris-HCl pH 8.0, 0.15 M NaCl, 2 mM EDTA, 1% TX-100, protease inhibitors) and precleared with Affi-Prep Protein A support beads (Bio-Rad). The respective antibodies were incubated with the lysate overnight at 4°C, followed

(2) YAP/TAZ and ATF4 drive resistance to Sorafenib in hepatocellular carcinoma by preventing ferroptosisEMBO Molecular MedicineDecember 7, 2021Ruize Gao et al.11815) overnight at 4°C. Immunocomplexes were incubated with 30 μl pre‐locked Sepharose Protein A beads (Affi‐Prep Protein A Support, Bio‐Rad; 1560006) at 4°C, 2 h later the immunocomplexes were centrifuged at 9,500 g for 1 min and the

(3) Modulation of Respiration and Mitochondrial Dynamics by SMAC-Mimetics for Combination Therapy in Chemoresistant CancerTheranosticsJuly 9, 2019Judith Hagenbuchner, Herbert Oberacher, Kathrin Arnhard, Ursula Kiechl-Kohlendorfer, Michael J. Ausserlechnereither 1 µg anti-Survivin antibody, anti-XIAP antibody or normal IgG as control covalently coupled to Affi-Prep Protein A support (Bio-Rad, Munich, Germany). Total protein (50 µg/lane), cytoplasmic and mitochondrial fractions (20

(4) The tubulin inhibitor MG-2477 induces autophagy-regulated cell death, ROS accumulation and activation of FOXO3 in neuroblastomaOncotargetMay 9, 2017Judith Hagenbuchner, Lorena Lungkofler, Ursula Kiechl-Kohlendorfer, Giampietro Viola, Maria Grazia Ferlin, Michael J. Ausserlechner, Petra ObexerBCLXL, (Cell Signaling Technology Inc., Boston, USA) or normal IgG control were covalently coupled to Affi-Prep Protein A Support (BioRad Laboratories, Munich, Germany) using dimethylpimelidate dihydrochloride/Borax buffer as described

(5) A CDC42EP4/septin-based perisynaptic glial scaffold facilitates glutamate clearanceNature communicationsDecember 10, 2015Ageta-Ishihara N, Yamazaki M, Konno K, Nakayama H, Abe M, Hashimoto K, Nishioka T, Kaibuchi K, Hattori S, Miyakawa T, et al.protease inhibitors), centrifuged and the supernatant was mixed with protein A Sepharose CL-4B (GE Healthcare) or Affi-Prep Protein A Support (Bio-Rad) that had been preincubated with anti-CDC42EP4, anti-SEPT4 or nonimmune rabbit IgG (as a negative

(6) CRL4(RBBP7) is required for efficient CENP-A deposition at centromeresJournal of Cell ScienceMay 1, 2015Julien Mouysset, Samuel Gilberto, Michelle G. Meier, Fabienne Lampert, Mukta Belwal, Patrick Meraldi, Matthias Peterimmunoprecipitate endogenous CUL4A, HeLa ‘Kyoto’ cells were lysed as described above and incubated with Affi-Prep Protein A Support resin (Biorad). Rabbit IgG and rabbit anti-CUL4A antibodies (Olma et al., 2009) were incubated for 1

(7) A Tetrahymena Hsp90 co-chaperone promotes siRNA loading by ATP-dependent and ATP-independent mechanismsThe EMBO JournalFebruary 12, 2015Sophie L Woehrer, Lucia Aronica, Jan H Suhren, Clara Janaâ€Lui Busch, Tomoko Noto, Kazufumi Mochizukiμl of water and stored at −20°C. Preparation of antibody-immobilized beads A 200 μl suspension of Affi-Prep Protein A Support (Bio-Rad) was washed twice with 500 μl of 1× loading buffer (30 mM HEPES-KOH pH 7.4, 40 mM KOAc, and

(8) A Cohesin-Independent Role for NIPBL at Promoters Provides Insights in CdLSPLoS GeneticsFebruary 13, 2014Jessica Zuin, Vedran Franke, Wilfred F. J. van IJcken, Antoine van der Sloot, Ian D. Krantz, Michael I. J. A. van der Reijden, Ryuichiro Nakato, Boris Lenhard, Kerstin S. WendtMgCl2, 0.2% NP40, 1 mM NaF, 0.5 mM DTT) and incubated for 1 h at 4°C with the respective antibodies. Affi-Prep Protein A support beads (BioRad) were added and incubated 1 h at 4°C. The beads were washed 3 times with IP buffer and

(9) Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cellsMolecular biology of the cellJanuary 1, 2014Gallego-Paez LM, Tanaka H, Bando M, Takahashi M, Nozaki N, Nakato R, Shirahige K, Hirota T.Wendt et al., 2008). In brief, cross-linked cell lysates solubilized by sonication were incubated with Affi-prep protein A Support (Bio-Rad) and cross-linked with the antibodies for 14 h at 4°C. After this, beads were washed several

(10) A novel Mcl1 variant inhibits apoptosis via increased Bim sequestrationOncotargetAugust 31, 2013Judith Hagenbuchner, Ursula Kiechl-Kohlendorfer, Petra Obexer, Michael J. Ausserlechnernegative control, were covalently coupled to Tachisorb™ Immunoadsorbent (Calbiochem, Nottingham, UK) or Affi-Prep Protein A Support (BioRad Laboratories, Munich, Germany) using dimethylpimelidate dihydrochloride/Borax buffer. Antibody-bead

(11) A novel Mcl1 variant inhibits apoptosis via increased Bim sequestrationOncotargetJuly 15, 2013Judith Hagenbuchner, Ursula Kiechl-Kohlendorfer, Petra Obexer, Michael J. Ausserlechnernegative control, were covalently coupled to Tachisorb™ Immunoadsorbent (Calbiochem, Nottingham, UK) or Affi-Prep Protein A Support (BioRad Laboratories, Munich, Germany) using dimethylpimelidate dihydrochloride/Borax buffer. Antibody-bead

(12) A hierarchy of signals regulates entry of membrane proteins into the ciliary membrane domain in epithelial cellsThe Journal of cell biologyApril 4, 2011Francis SS, Sfakianos J, Lo B, Mellman I.constructs IP protocol is based on Cheeseman and Desai (2005). For each IP, 160 µl of resuspended Affi-Prep Protein A Support (Bio-Rad Laboratories) bead slurry (∼110-µl bead volume) was used to make anti-GFP beads. Volumes were

(13) Regulation of RB transcription in vivo by RB family membersMolecular and cellular biologyApril 1, 2010Burkhart DL, Ngai LK, Roake CM, Viatour P, Thangavel C, Ho VM, Knudsen ES, Sage J.Cells were lysed, and nuclear extracts were sonicated through 12 30-s pulses on a Vircell sonicator at 4°C. Affi-Prep protein A support (Bio-Rad) was blocked overnight with salmon sperm DNA and BSA. Immunoprecipitations were performed overnight

(14) Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg ExtractsMethods in Molecular BiologyJuly 21, 2009Michael J. Emanuele, P. Todd Stukenberg7.5TBSt: 10 mM Tris, 137 mM NaCl, 0.1% Tween-20, pH 7.5Bovine Serum Albumin, Fraction V (lyophilized)Affi-Prep Protein-A Support (Bio-Rad: Cat # 156-0006)12 well tissue culture dishes (COSTAR: Cat #- 3513)Antibodies- used for immunodepletion

(15) Maturation of active zone assembly by Drosophila BruchpilotThe Journal of cell biologyJuly 13, 2009Fouquet W, Owald D, Wichmann C, Mertel S, Depner H, Dyba M, Hallermann S, Kittel RJ, Eimer S, Sigrist SJ.total protein extract from whole cell lysates was mixed with 20 µl protein A agarose bead suspension (Affi-prep protein A support; Bio-Rad Laboratories) precoupled with either monoclonal Mα-Myc antibody (9E10; Santa Cruz Biotechnology,

(16) Shugoshin regulates cohesion by driving relocalization of PP2A in Xenopus extractsChromosomaApril 1, 2009Teresa Rivera, Ana Losada(two rounds of 40 min). For the depletion of Sgo, 50 μl of rabbit polyclonal serum was bound to 25 μl of Affi-prep protein A Support (BIO-RAD). In all cases, mock depletions were performed using beads coated with pre-immune rabbit IgG (Sigma). Chromosome

(17) Regulation of Microtubule Assembly and Organization in Mitosis by the AAA+ ATPase PontinMolecular Biology of the CellJuly 1, 2008Daniel Ducat, Shin-ichi Kawaguchi, Hongbin Liu, John R. Yates, Yixian Zheng␥-tubulin (Zheng et al., 1995), for 1 h with rotation at 4°C. The immune-complex was recovered using Affi-Prep protein A support beads (Bio-Rad, Hercules, CA). After washing the beads three times with HB100K, ␥TuRC was eluted using

(18) Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2The EMBO JournalNovember 29, 2006Anja Hanisch, Herman H W Silljé, Erich A Niggand Western blot analysis were described previously (Hanisch et al, 2006). For immunoprecipation experiments Affi-Prep Protein A Support beads (Bio-Rad Laboratories, Hercules, CA) coated with either rabbit anti-Ska1, rabbit antiSka2 or rabbit

(19) ZW10 links mitotic checkpoint signaling to the structural kinetochoreJournal of Cell BiologyApril 11, 2005Geert J.P.L. Kops, Yumi Kim, Beth A.A. Weaver, Yinghui Mao, Ian McLeod, John R. Yates, Mitsuo Tagaya, Don W. Clevelanddescribed for the affinity purification, using rabbit anti-GFP antibody coupled and cross-linked to Affi-Prep protein A support (Bio-Rad Laboratories). The matrix was washed and resuspended in 2× Laemmli sample buffer. SDS-PAGE

(20) A role of topoisomerase II in linking DNA replication to chromosome condensationThe Journal of Cell BiologyMarch 3, 2003Olivier Cuvier, Tatsuya Hiranoaffinity-purified anti-XCAP-C, -E, and -G (6 μg each) and anti-XCAP-D2 (12 μg) was incubated with 30 μl Affi-Prep® Protein A support (Bio-Rad Laboratories) for 1 h. For topo II depletion, 30 μl of anti-topo II serum was loaded onto 30

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