Micro Bio-Spin™ Chromatography Columns from Bio-Rad

Product Specs
ItemMicro Bio-Spin™ Chromatography Columns
CompanyBio-Rad
Price $165.00Supplier Page View Company Product Page
Catalog Number7326204
QuantityPkg of 100
TypeColumns

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Protocol to identify covalent inhibitors targeting RhoA Cys16STAR ProtocApril 14, 2026Tin-Yan Koo, Chloe See-Hang Ho, Hillary Yui-Yan Yip, Clive Yik-Sham ChungV5111 C18 Stage Tips,Thermo Scientific™,Cat# 87782 Micro Bio-Spin Chromatography Columns,Bio-Rad,Cat# 7326204 Aurora C18 UHPLC column (75 μm i.d. × 25 cm length × 1.6 μm particle size),IonOpticks Australia,https://ionopticks.com/product/aurora-ultimate-25cm-x-75um-c18-uhplc-column/

(2) A bacterial family of fatty acid acyltransferases related to the Shigella effector IcsBmBioApril 8, 2026Waad Bajunaid, Kyle Tomaro, Anwer Hasil Kottarampatel, Geneviève F. Desrochers, Mathieu Lavallée-Adam, John P. Pezacki, François-Xavier Campbell-Valoisbead slurry (Thermo Scientific, 20353) was washed 3× with 700 μL PBS in a biospin column (Bio-Rad, 7326204) at 1,000 × g for 1 min. Beads were then transferred to protein samples using 500 μL PBS and rotated

(3) Chiral methionine oxidation reagents reveal stereospecific proteome modificationsbioRxivMarch 30, 2026Angel Gonzalez-Valero, Annika C. S. Page, Jayde M. Bertoch, Fadi Alsarhan, Jaehee Kim, Amr A. Alazali, Ritwik R. Srinivas, Xiao Xie, Audrey G. Reeves, Kacper Skakuj, et al.beads were then washed with 500 μL PBS (5X) and 500 μL MQ (5X) in micro bio-spin columns (Bio-Rad; 7326204) on a vacuum manifold. With the final wash, samples were transferred to a fresh 1.7 mL microcentrifuge

(4) Development of DNA Aptamers Against Leishmania infantum GP63 Protein for Therapeutic and Diagnostic ApplicationsPharmaceuticsFebruary 28, 2026Lucía Román-Álamo, Daniela Currea-Ayala, Gabriel S. Oliveira, Antonino Nicolò Fallica, Timen Mooren, Yunuen Avalos-Padilla, Xavier Fernàndez-Busquetsquantified in a Nanodrop 2000 (Thermo Fisher Scientific), and the forward single strand was purified in Micro Bio-Spin chromatography columns (Bio-Rad Laboratories, Inc.) loaded with High Capacity NeutrAvidinTM Agarose (Thermo Fisher Scientific),

(5) Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domainsMolecular CellJanuary 8, 2026Lin Guo, Jacob R. Mann, Jocelyn C. Mauna, Katie E. Copley, Hejia Wang, Jack D. Rubien, Cristian A. Bergmann, Jenny L. Carey, Jessica Merjane, Marilyn Ngo, et al.TDP-43-MBP-his was buffer exchanged into 166.66mM NaCl, 22.22mM HEPES-NaOH pH 7.0, 1.11mM DTT (Bio-Rad Micro Bio-Spin Chromatography Columns, following manufacturer’s instructions) and concentration was determined via NanoDrop, e280=114250 cm−1M−1.

(6) Anionic Detergents as Eluents for Microscale Isolation of Antigen-Specific Serum ImmunoglobulinsBiosensors (Basel)December 28, 2025Dmitry Trukhin, Marina Filippova, Alla Tskaeva, Ekaterina Troshina, Dmitry Gryadunov, Elena SavvateevaImmunoglobulins 2.5.1. Gel Filtration The obtained eluates (100 µL) were applied to spin columns (7326204, Bio-Rad Laboratories, USA) filled with Sephadex G-25 coarse (17-0034-02, GE Healthcare, Chicago, IL,

(7) A non-enzymatic role of Nudix hydrolase 5 in repressing purine de novo synthesisScienceDecember 11, 2025Tuan-Anh Nguyen, Jung-Ming G. Lin, Anne-Sophie M. C. Marques, Maximilian Fottner, Ludwig G. Bauer, Andreas Reicher, Diana Daum, Lorenzo Scrofani, Yusi Liu, Carol Cheng, et al.After 1 h incubation at 4°C while being rotated, beads were transferred to a Mini Spin column (BioRad, 7326204) and washed twice with wash buffer (lysis buffer without protease inhibitor) and once with Tris-HCl

(8) Single-cell mapping of the glycocalyx in the adult human and mouse ocular surfacePNAS NexusOctober 31, 2025Rafael Martínez-Carrasco, Pablo Argüeso(Thermo Fisher Scientific) according to the manufacturer's instructions. The beads were loaded into Micro Bio-Spin chromatography columns (Bio-Rad, Hercules, CA, USA). Conjugated lectins were dissolved in up to 200 µL of Dulbecco's phosphate-buffered

(9) Phosphate ions modulate enzyme activity and epistatic effects in two clavulanic acid-resistant β-lactamase mutantsProtein SciOctober 11, 2025Marko Radojković, Saar F. Koene, Aleksandra Chikunova, Bogdan I. Florea, Sivanandam V. Natarajan, Aimee L. Boyle, Marcellus Ubbinkenzyme‐to‐inhibitor ratio). After thawing, the sample buffer was exchanged for 10 mM ammonium acetate, pH 6.8, using Micro Bio‐Spin chromatography columns (Bio‐Rad). Samples were then loaded onto a Silica‐C4 reversed phase UPLC column and analyzed using a

(10) Mannose trimming is the dominant signal for the release of misfolded glycoproteins from ER quality controlJournal of Biological ChemistryOctober 1, 2025Yun-Ji Shin, Ulrike Vavra, Daniel Maresch, Clemens Grünwald-Gruber, Richard StrasserCA-630, followed by one washing step using the same buffer solution. The beads were then transferred to Micro Bio-Spin chromatography columns (Bio-Rad) and washed twice with 600 μl of a buffer solution containing 10 mM Tris–HCl, pH 7.5, 150 mM

(11) Molecular determinants for the endocytosis of the voltage-gated K + channel Kv1.3Science SignalingSeptember 30, 2025Irene Estadella, María Navarro-Pérez, Magalí Colomer-Molera, Michael L. Dustin, Alexander Sorkin, Jesusa Capera, Antonio Felipemixing. The beads were removed by centrifugation at 1000g for 30 s. Samples were incubated overnight in Micro Bio-Spin Chromatography Columns (Bio-Rad) with 50 μl of protein A-Sepharose beads and anti–green fluorescent protein (GFP) (4 ng of

(12) Classically activated macrophages undergo functionally significant nucleotide metabolism remodelling driven by nitric oxideNature MetabolismAugust 4, 2025Steven V. John, Gretchen L. Seim, Billy J. Erazo-Flores, James A. Votava, Uzziah S. Urquiza, Nicholas L. Arp, John Steill, Jack Freeman, Lauren N. Carnevale, Isaiah Roberts, et al.25°C. The total volume was spun at 1,000 × g for 4 minutes at 4°C in a chromatography column (BioRad: 7326204). The flowthrough was transferred to a 30 kDa MWCO filter (Sigma Aldrich: UFC503024). 300 μL of storage

(13) Structures of Mycobacterium tuberculosis isoprenyl diphosphate synthase Rv2173 in substrate-bound formsActa Crystallographica Section F Structural Biology CommunicationsMay 1, 2025James A. Titterington, Ngoc Anh Thu Ho, Charles P. H. Beasley, Francis Mann, Edward N. Baker, Timothy M. Allison, Jodie M. Johnstonanalysis, the protein sample (0.2 mg ml−1) was buffer-exchanged into 0.75 M ammonium acetate using Micro Bio-Spin Chromatography Columns (Bio-Rad) and then centrifuged at 17 000g for 2 min before dispensing into an electrospray capillary.

(14) Antibiotic target discovery by integrated phenotypic and activity-based profiling of electrophilic fragmentsCell Chemical BiologyMarch 20, 2025Yizhen Jin, Sadhan Jana, Mikail E. Abbasov, Hening Linambient temperature. Samples were next transferred onto Micro Bio-Spin Chromatography Columns (Bio-Rad, 7326204) and washed with the wash buffer (3 × 50 mM TEAB, 150 mM NaCl, 0.1% Igepal CA-630), DPBS (3×), and water

(15) Sulfinyl Aziridines as Stereoselective Covalent Destabilizing Degraders of the Oncogenic Transcription Factor MYCbioRxiv : the preprint server for biologyFebruary 27, 2025Rosen HT, Li K, Li EL, Currier B, Brittain SM, Garcia FJ, Beard DC, Haenni-Holzinger S, Dovala D, McKenna JM, et al.streptavidin-agarose beads (ThermoFisher, 20353) were washed with PBS using a Micro-Bio Spin Column (Bio-Rad, 7326204) and vacuum manifold. 500 μL of PBS was added to the dissolved proteins, and then the washed beads were

(16) Molecular mechanisms of CAND2 in regulating SCF ubiquitin ligasesNat CommunFebruary 26, 2025Kankan Wang, Lihong Li, Sebastian Kenny, Dailin Gan, Justin M. Reitsma, Yun Zhou, Chittaranjan Das, Xing Liubuffer and 100 mM Tris-HCl (pH 8.0). The resin was subsequently transferred to a spin column (Bio-Rad, 7326204) and incubated twice with 150 µL of 10% ammonium hydroxide for 5 min to elute the bound proteins. The

(17) Specialized metabolome and transcriptome atlas of developing Arabidopsis thaliana seed under warm temperaturesSci DataFebruary 20, 2025Léa Barreda, Stéphanie Boutet, Delphine De Vos, Céline Boulard, Damaris Grain, Loïc Lepiniec, Massimiliano Corsoand RNAs dried prior to their resuspension in 40 µL of RNAse-free water. RNAs were purified by using Micro Bio Spin Chromatography columns (BioRad) previously pre-conditioned by adding 5 mg of PVPP and two consecutive washings with 400 µL

(18) Force-bearing phagocytic adhesion rings mediate the phagocytosis of surface-bound particlesNature communicationsJanuary 24, 2025Kundu S, Pal K, Pyne A, Wang X.diffuses to PBS and is extracted by spinning the diced gel solution through a column with a filter (7326204, Bio-Rad). The purification with electrophoresis can be skipped if the RGD-DNA purity checked by a low-scale

(19) Chemical tools to define and manipulate interferon-inducible Ubl protease USP18Nat CommunJanuary 22, 2025Griffin J. Davis, Anthony O. Omole, Yejin Jung, Wioletta Rut, Ronald Holewinski, Kiall F. Suazo, Hong-Rae Kim, Mo Yang, Thorkell Andresson, Marcin Drag, et al.washed (4× 0.2% NP-40/PBS, 4x PBS, 4x ddH2O, 1x HEPES) by centrifugation (500 × g, 1 min, 4 °C) with Micro Bio-Spin™ Chromatography Columns (Bio-rad). After the final wash, the supernatant was removed, 50 μL of 100 mM of HEPES pH 7.4 was added,

(20) A Label-Free Colorimetric Aptasensor for Flavokavain B DetectionSensors (Basel)January 19, 2025Sisi Ke, Ningrui Wang, Xingyu Chen, Jiangwei Tian, Jiwei Li, Boyang Yu(Grand Island, NY, USA). Streptavidin-coated agarose resin was obtained from Thermo Fisher Scientific. Micro Bio-Spin chromatography columns (500 μL) were sourced from Bio-Rad, and 2 × GoTaq Hot Start Colorless Master Mix was acquired from Promega.

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