HaloLink Resin from Promega

Product Specs
ItemHaloLink Resin
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG1914
Quantity10ml
TypeResins
ModeAffinity Purification
Applicationspurification

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) The COX2-PGE2-PKA Axis Suppresses Antiviral Immunity by Inhibiting mtDNA-Dependent STING ActivationbioRxivApril 6, 2026Pham Thuy Tien Vo, Julien Cicero, Zichen Wang, Hiroyuki Hakozaki, Thomas S Hoang, Sendi Rafael Adame-Garcia, Dana J. Ramms, Kuniaki Sato, Erica Stevenson, Yuan Zhou, et al.20μL of 8-AHA-cAMP- or Rp-8-AHA-cAMPS-agarose (Biolog, A012 and A028) or HaloLink-agarose (Promega, G1914) as a negative binding control, with continuous rotation at 4°C for 3 hours. A 75 μL aliquot of the

(2) Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stressSci AdvMarch 6, 2026Kenneth Ehses, Jorge P. López-Alonso, Odetta Antico, Yannik Lang, Till Rudack, Abdussalam Azem, Miratul M. K. Muqit, Iban Ubarretxena-Belandia, Rubén Fernández-Busnadiegom-DSK, a yeast Dsk1-derived ubiquitin-binding protein, was immobilized by incubating with 200 μl of HaloLink resin (Promega) in binding buffer [50 mM tris-HCl (pH 7.5), 150 mM NaCl, and 0.05% NP-40] overnight at 4°C.

(3) E3 ligase LMO7 enhances temozolomide sensitivity by promoting MGMT degradation in lung cancerJournal of Biological ChemistryFebruary 26, 2026Jiabing Li, Xiaorong Feng, Yunfang Deng, Bei Chen, Lin Miao, Zhaohui Liu, Zhiming Sun, Lei Hu, Jinqiu Ma, Liyuan Zeng, et al.a final SDS concentration of 0.1% (w/v). Cell extracts were incubated with 10 μl of HaloLink resin (G1915, Promega) prebound with 10 μg of purified Halo-TUBE protein per sample. Incubation was carried out overnight

(4) Structural Mechanisms of SAMD9 Autoinhibition and Pathogenic DysregulationbioRxivFebruary 3, 2026Zongjun Mou, Fushun Zhang, Marisol Morales, Bibekananda Sahoo, Xinghong Dai, Yan XiangDTT and 0.005% IGEPAL CA-630). Clarified lysates were incubated with 500 μl HaloLink Resin (Promega G1915) pre-equilibrated in HaloTag Protein Purification Buffer. Binding was performed by inversion mixing

(5) Protein kinase a regulates cyclooxygenase-2 expression through the RNA-binding proteins HuR and TTPJournal of Biological ChemistryFebruary 1, 2026Sendi Rafael Adame-Garcia, Thomas S. Hoang, Pham Thuy Thien Vo, Valeria Burghi, Rodolfo Daniel Cervantes-Villagrana, Lennis Beatriz Orduña-Castillo, Dana J. Ramms, JoAnn Trejo, J. Silvio Gutkind20 μl of 8-AHA-cAMP- or Rp-8-AHA-cAMPS-agarose (Biolog, A012 and A028) or HaloLink-agarose (Promega, G1914) as a negative binding control, with continuous rotation at 4 °C for 3 h. A 75 μl aliquot of the pooled

(6) Dissecting the Interaction Domains of SARS-CoV-2 Nucleocapsid Protein and Human RNA Helicase DDX3X and Search for Potential InhibitorsInternational Journal of Molecular SciencesJanuary 9, 2026Camilla Lodola, Maria Michela Pallotta, Fabrizio Manetti, Paolo Governa, Emmanuele Crespan, Giovanni Maga, Massimiliano Secchimixing 3 µg of Halo tagged proteins with 3 µg of NLuc tagged proteins and 50 µL of pre-equilibrated HaloLink resin (Promega) to reach a total volume of 500 µL in TBST 1× with the addition of 20 μg/mL DNaseI and of 100

(7) Carbonic Anhydrase 3 Overexpression Modulates Signalling Pathways Associated with Cellular Stress Resilience and ProteostasisInt J Mol SciDecember 15, 2025Yezhou Yu, Merrina Anugraham, Tony Blick, Arutha Kulasinghe, Louise M. Sternicki, Giovanna Di Trapani, Sally-Ann Poulsen, Daniel Kolarich, Kathryn F. Tonissenwas transferred into a new tube and diluted with 700 µL of 1× Tris-buffered saline pH 7.5 (Promega). HaloLink Resin (200 µL, Promega) was spun down (800× g, 1 min) and washed with 800 µL of Resin Equilibration/Wash Buffer

(8) The deubiquitinase OTUD4 suppresses TAK1 kinase–dependent NF-κB signaling and inflammationJournal of Biological ChemistryNovember 1, 2025Zhaohui Liu, Xiaolong Wang, Lihui Wu, Li Min, Ying Sheng, Zhiming Sun, Yunfang Deng, Lin Miao, Yue Liu, Jiabing Li, et al.affinity purification. Cell extracts were incubated overnight at 4 °C with 10 μl of HaloLink resin (G1915; Promega) prebound to purified Halo-NZF2 protein (7 μg/sample) under gentle rotation. After incubation,

(9) Distinct Filament Conformation for Receptor-Bound Amyloid-ß from Alzheimer’s Disease BrainbioRxivOctober 16, 2025Mikhail A. Kostylev, Carmen Butan, Graham P. Roseman, Yangyi Liu, Pallavi Gopal, Stephen M. Strittmatterconjugate purified IC16 to a resin, the antibody was diluted to 0.25 mg/mL with PBS and mixed with HaloLink Resin (Promega) (1 mg of IC16 to 1 mL of resin) for at least four hours at room temperature. Conjugation was

(10) Identification of a RAB32-LRMDA-Commander membrane trafficking complex reveals the molecular mechanism of human oculocutaneous albinism type 7Nat CommunOctober 2, 2025Rebeka Butkovič, Michael D. Healy, Cecilia de Heus, Alexander P. Walker, Wyatt Beyers, Kerrie E. McNally, Philip A. Lewis, Kate J. Heesom, Nalan Liv, Judith Klumperman, et al.and added to pre-washed Glutathione Sepharose 4b beads (Cytiva, 17075601) or HaloLink resin (Promega, G1914). The samples were incubated for 1 h at room temperature, and following the incubation, beads were collected

(11) SPIDR enables multiplexed mapping of RNA-protein interactions and uncovers a mechanism for selective translational suppression upon cell stressCellSeptember 18, 2025Erica Wolin, Jimmy K. Guo, Mario R. Blanco, Isabel N. Goronzy, Darvesh Gorhe, Wenzhao Dong, Andrew A. Perez, Abdurrahman Keskin, Elizabeth Valenzuela, Ahmed A. Abdou, et al.cleared by centrifugation at 15000 g for 2 minutes and the supernatant was collected for capture to HaloLink Resin (Promega). 50 μL of lysate was taken prior to immunoprecipitation for input processing. For each CLAP

(12) USP7 promotes temozolomide resistance by stabilizing MGMT in glioblastomaCell Death DisAugust 20, 2025Jiabing Li, Xiaorong Feng, Zhaohui Liu, Yunfang Deng, Zhiming Sun, Bei Chen, Lihui Wu, Xiaolong Wang, Lin Miao, Liyuan Zeng, et al.concentration to 0.1% prior to affinity purification. Extracts were incubated with 10 μL of HaloLink™ resin (G1915, Promega) pre-bound to purified Halo-TUBE protein (10 μg per sample). The clarified extracts were incubated

(13) CCDC32 collaborates with the membrane to assemble the AP-2 clathrin adaptor complexbioRxivAugust 5, 2025Dillon E. Sloan, Ariel Matthews, Haruaki Yanagisawa, Tanita Tedamrongwanish, Kevin Cannon, Jake Simmons, Garrett Chappell, Nathan I. Nicely, Rebecca Berlow, Masahide Kikkawa, et al.of 100 μL base buffer (20 mM HEPES, pH 7.6, 100 mM NaCl, 1 mM DTT, 0.1% Tween-20). 10 μL of magnetic Halolink resin (20% slurry, Promega) was added to 200 μL tubes and washed 5x in base buffer before proteins were added.

(14) A sperm–oocyte protein partnership required for egg activation in Caenorhabditis elegansDevelopmentJune 15, 2025Tatsuya Tsukamoto, Ji Kent Kwah, Mark E. Zweifel, Naomi Courtemanche, Micah D. Gearhart, Katherine M. Walstrom, Aimee Jaramillo-Lambert, David GreensteinHaloTag protein purification buffer (50 mM HEPES, pH 7.5, 150 mM NaCl, 0.05% NP-40) and applied to HaloLink resin (Promega) in batch overnight at 4°C plus 1 h at room temperature. After the binding, the unbound proteins

(15) MicroRNA mechanisms instructing Purkinje cell specificationNeuronMay 21, 2025Norjin Zolboot, Yao Xiao, Jessica X. Du, Marwan M. Ghanem, Su Yeun Choi, Miranda J. Junn, Federico Zampa, Zeyi Huang, Ian J. MacRae, Giordano Lippiflash-frozen in liquid N2, and stored at −80 °C. Preparation of Halo-SpyCatcher3 Resin 400 μL of HaloLink™ resin (Promega, G1912) was washed three times with Halo binding buffer (1x PBS with 0.005% IGEPAL® CA-630

(16) The Porphyromonas gingivalis RNA-binding protein is required for growth in high levels of zinc and persistence with host cellsFront Cell Infect MicrobiolMay 15, 2025Sai Yanamandra, Holly Marsh, Romana Cvitkovic, Qin Gui, Benjamin R. Belvin, Janina P. Lewis(Sigma-Aldrich). The cell lysates were centrifuged at 12,000 rpm for 15 min, and supernatant was applied to HaLoLink resin (Promega). Following 45 min of incubation at room temperature, the resin-tagged protein was washed three

(17) Zic family member 5 promotes RIO kinase 3 expression to enhance pancreatic cancer survivalThe FEBS JournalMay 3, 2025Reiko Satow, Yuki Kashiwaba, Misaki Okao, Shin Takano, Yuna Aiga, Atsuko Yoneda, Kazuyoshi Hosomichi, Kiyoko Fukamiconcentration of 125 mm for 10 min. Cells were processed according to the manufacturers' protocol using HaloLink™ Resin (Promega, Tokyo, Japan). The protein–DNA complexes were then reverse‐crosslinked by heating for 6 h

(18) ATLAS: a rationally designed anterograde transsynaptic tracerNature methodsMay 1, 2025Rivera JF, Huang H, Weng W, Sohn H, Girasole AE, Li S, Albanese MA, Qin M, Tao C, Klug ME, et al.secreted protein AF-HA-TEVcs-HALOTag was collected and incubated with HaloLink resin (Promega, cat. no. G1914) overnight at 4 °C. and then with tobacco etch virus (TEV) protease (New England Biolabs, cat. no. P8112S)

(19) Serum/glucose starvation enhances binding of miR-4745-5p and miR-6798-5p to HNRNPA1 mRNA 3ʹUTR: A novel method to identify miRNAs binding to mRNA 3ʹUTR using λN peptide-boxB sequenceNon-coding RNA ResearchApril 1, 2025Tetsuyuki Takahashi, Mai Funamura, Shun Wakai, Takao HijikataNP-40, and 0.5 mM DTT) supplemented with RNaseOUT (Invitrogen, Carlsbad, CA, USA) and pulled down with HaloLink™ Resin (Promega) for 1 h at 25 °C. RNAs from the resultant precipitate were isolated using ISOGEN (Nippon Gene,

(20) SIRT7 Is a Lysine Deacylase with a Preference for Depropionylation and DemyristoylationInternational Journal of Molecular SciencesMarch 28, 2025Mohammad Golam Kibria, Tatsuya Yoshizawa, Tianli Zhang, Katsuhiko Ono, Tomoya Mizumoto, Yoshifumi Sato, Tomohiro Sawa, Kazuya Yamagatafor 10 min at 4 °C. Then, 5 mL cleared lysate was incubated with 50 µL pre-washed HaloLink resin (#G1914; Promega) overnight at 4 °C. The resin was washed with Halo purification buffer, and ProTEV Plus protease

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HaloLink Resin

HaloLink Resin from Promega

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HaloLink Resin from Promega

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