Y-27632 Dihydrochloride from Selleck Chemicals

Product Specs
ItemY-27632 Dihydrochloride
CompanySelleck Chemicals
Price $570.00Supplier Page View Company Product Page
Catalog NumberS1049
Quantity50mg
Purity99.86
TargetROCK
Storage3 years-20°C powder
FormulaC14H21N3O.2HCl

Specifications/Features

Ki, IC, EC, or working concentration: Ki: 0.14 µM for p160ROCK
Ki: 26 µM for PKC
Ki: 25 µM for cAMP-dependent protein kinase
Ki: >250 µM for myosin light-chain kinase

Add to Compare List

Selleck Chemicals

14408 W Sylvanfield Drive

Houston, Texas TX 77014

United States

Phone: Toll Free: (877) 796-6397 (US and Canada only)
Tel: (832) 582-8158
Fax: (832) 582-8590

Email: sales@selleckchem.com

Company Profile

Website: www.selleckchem.com

(1) E- and N-cadherin drive hepatic polarity and lumen elongation via opposing effects on RhoA activityJ Cell BiolAugust 3, 2026Junya Hayase, Li Yang, Yu-Heng Zhou, Kangji Wang, Cheng-Ran Xu, Erfei Biliver explants were cultured for 2 days in the presence of DMSO (D2650; Sigma-Aldrich) or 1 µM Y27632 (S1049; Selleck). Antibodies and chemicals Anti-E-cadherin (36/E-cadherin, 610182) and anti-N-cadherin (32/N-cadherin,

(2) Global transcriptional changes across multiple isogenic C9orf72 patient iPSC-derived neuronsiScienceJune 19, 2026Aparna Sreeram, Desiree M. Baron, Alberto Brusati, Karly Stallworth, Jack Humphrey, John E. LandersBP531-500 Matrigel hESC-Qualified Matrix,Corning,Cat# 08-774-552 ROCK Inhibitor,Selleck Chemicals,Cat# S1049 Lipofectamine Stem Transfection Reagent,Invitrogen,Cat# STEM00001 Stemflex Medium,Gibco,Cat# A3349401 Poly-L-Ornithine,Sigma,Cat#

(3) Arrayed dual-gRNA CRISPR Screening Platform for C9orf72 Repeat Expansion Excision in Patient iPSCsMolecular Therapy AdvancesJune 11, 2026Olubankole Aladesuyi Arogundade, Katie Jing Kay Kay Lam, Katherine A. Brown, Tanya Jain, Patrick O. Issagholian-Lewin, Cerianne Huang, Taylor Rae-Hudson, Kevin Briseno, Stacia K. Wyman, Netravathi Krishnappa, et al.one well of a 24-well plate and were recovered in mTeSR Plus with 10 μM ROCK1 inhibitor (Selleckchem S1049) and CloneR (STEMCELL Technologies 05888). We collected the pool of edited cells after 48 h and extracted

(4) Protocol for generating iPSC-derived intestinal organoids through dissection to model cystic fibrosis therapiesSTAR ProtocolsJune 1, 2026Alexander J. Stuffer, Barbara Tabak, Joshua Conte, Victoria Momyer, Mercy Chado, Matthew Armstrong, Phillip Wible, Andrey Sivachenko, Normand Allaire, Junjie Lu, et al.,R&D Systems,251-KG-050/CF Dexamethasone,Millipore Sigma,D4902 3-Isobutyl-1-methylxanthine (IBMX),Millipore Sigma,I5879 Y-26732,Selleck Chemicals,S1049 "8-Bromoadenosine 3′,5′-cyclic monophosphate sodium salt (cAMP)",Millipore Sigma,B7880 HEPES (1 M),Thermo Fisher Scientific

(5) Phosphoproteomics distinguishes disease-specific mechanisms for human phospholamban cardiomyopathy reversible by RNA therapySignal Transduct Target TherMay 28, 2026Frederik E. Deiman, Nils Bömer, Pia Davidsson, Daniela Später, Annet N. Linders, Itamar B. Dias, Karla F. Arevalo Gomez, Antonio Esquivel Gaytan, Jumo Zhu, Anna Walentinsson, et al.#12604-021) and plated as single cells in Essential 8 medium with 5 µM Y27632 (Selleck Chemicals, #S1049). The medium was refreshed daily. Upon reaching 80% confluency, differentiation was initiated on day

(6) Knockout of the LRRK2-counteracting RAB phosphatase PPM1H disrupts axonal autophagy and exacerbates alpha-synuclein aggregationCell RepMay 21, 2026Michel Fricke, Anna Mechel, Lennart Evers, Björn Twellsieck, Jessica M. Grein, Maria-Sol Cima-Omori, Mohammed Al-Azzani, Tiago F. Outeiro, Markus Zweckstetter, Erika L.F. Holzbaur, et al.,Thermo Fisher,A1517001 ReLeSR,Stemcell Technologies,05872 Accutase,Sigma-Aldrich,A6964 ROCK Inhibitor Y-27632,Selleckchem,S1049 Knockout Serum Replacement,Thermo Fisher,10828010 "DMEM/F-12, HEPES",Thermo Fisher,11330032 N2 Supplement,Thermo Fisher

(7) A longitudinal single-cell and spatial multiomic atlas of pediatric high-grade gliomaCell Reports MedicineMay 19, 2026Jonathan H. Sussman, Derek A. Oldridge, Wenbao Yu, Chia-Hui Chen, Abigail M. Zellmer, Jiazhen Rong, Arianne Parvaresh-Rizi, Anusha Thadi, Austin Yang, Joseph S. Tumulty, et al.Hydrochloride,Selleckchem,S7060 SB366791,Selleckchem,S8238 Verbascoside,Cayman Chemicals,24965 Y-27632,Selleckchem,S1049 Optimal Cutting Temperature (OCT) Compound,Sakura Finetek,Cat# 4583 "Nuclei Extraction Buffer components

(8) Human pancreatic progenitor organoids define genetic and epigenetic barriers to early PDAC transformationDev CellMay 19, 2026Xian Zhang, Wilfred Wong, Hyein S. Cho, Dapeng Yang, Gary Dixon, Renhe Luo, Dingyu Liu, Denis Torre, Shigeaki Umeda, Federico González, et al.(Stem Cell Technologies, 05872). 5 μM Rho-associated protein kinase (ROCK) inhibitor Y-27632 (Selleck Chemicals, S1049) was added into the culture medium when passaging cells. H1 hPSCs (NIHhESC-10–0043) were maintained

(9) Adrenomedullin restores the human cortical interneurons migration defects induced by hypoxiaElifeMay 15, 2026Alyssa Puno, Wojciech P Michno, Li Li, Amanda Everitt, Kate McCluskey, Saw Htun, Dhriti Nagar, Jong Bin Choi, Yuqin Dai, Seyeon Park, et al.centrifuged at 100 × g for 3 min in hiPSC medium supplemented with ROCK inhibitor Y-27632 (Selleck Chemicals, S1049). After 24 hr, the newly formed organoids were transferred into ultra-low-attachment plastic dishes

(10) Functional chromatin signatures premark future lineage-specific enhancersCell GenomicsMay 13, 2026Julian Pulecio, Zakieh Tayyebi, Dingyu Liu, Wilfred Wong, Renhe Luo, Jeyaram R. Damodaran, Samuel J. Kaplan, Nan Hu, Hyein S. Cho, Jielin Yan, et al.dissociate cells. 5 μM Rho-associated protein kinase (ROCK) inhibitor (RI) Y-27632 (Selleck Chemicals, S1049) was added into the E8 media when passaging or thawing hESCs. Cells were tested every 6 months by Memorial

(11) Translational 3D in vitro models for immunotherapy testing: from reconstituted organoid co-culture assays to autologous ex vivo patient tissuesFront ImmunolMay 8, 2026Saskia de Man, Ezgi Kaya-Aksoy, Tomas Veenendaal, Niels Meesters, Sergei Chavez Abiega, Xuefei Yan, Michelle Kop, Luca Gandini, Jolie Flach, Jiawei Meng, et al.(ImmunoTools, cat# 11343045), 10 ng/mL hFGF-b (PeproTech, cat# 100-18B), 0.01 mM Y27632 (SelleckChem, cat# S1049) and 10 ng/mL hEGF (PeproTech, cat# AF-100-15) Therapeutics, including control and reference compounds,

(12) OGDHL promotes prostate cancer progression and regulates neuroendocrine marker expression and nucleotide abundanceMol Cancer ResMay 4, 2026Matthew J. Bernard, Andrea Gallardo, Angel Ruiz, Johnny A. Diaz, Nicholas M. Nunley, Rachel N. Dove, Shile Zhang, Ernie Lee, Kylie Y. Heering, Grigor Varuzhanyan, et al.mg/mL Dispase II (Gibco: 17-105-041) and 10μM p160 ROCK-inhibitor Y-27632 2HCl (Selleck Chemicals: S1049). This mixture was incubated at 37°C for 1 hour with constant rocking. Following dissociation, cells

(13) Predictive Cellular Signatures from Live Human Motor Neurons Distinguish TDP-43 ALS and Enable ALS Subtype StratificationbioRxivMay 1, 2026Julia Kaye, Naufa Amirani, Úna Chan, Noura Al Bistami, Zohreh Faghihmonzavi, Monika Ahirwar, Reuben Thomas, Wesley Robinson, Edward Vertudes, Krishna Raja, et al.motor neuron differentiation All iPSCs were thawed into media with ROCK inhibitor (ROCKi; Selleck Chemicals, #S1049) and mTESR Plus media (StemCell Technologies, # 00–1130). iPSCs were cultured in mTESR Plus and grown

(14) Isoginkgetin protects against degeneration of ALS motor neurons via regulating the GSK-3β–TFEB signaling axisPharmacological ResearchMay 1, 2026Ang Li, Xianglu Xiao, Guopan Liu, Krinos Li, Yixia Ling, Shenglong Deng, Chunzuan Xu, Shu-qin Cao, Jing Wen, Guang Lu, et al.neurotrophic factor (BDNF) (HY-P7116A; MCE), ciliary neurotrophic factor (CNTF) (HY-P7146; MCE), Y-27632 (S1049; SELLECKCHEM), and MG-132 (HY-13259; MCE). Primary antibodies used in this study were as follows: anti-TFEB

(15) THE FAM53C/DYRK1A axis regulates the G1/S transition of the cell cycleeLifeApril 30, 2026Taylar Hammond, Jong Bin Choi, Miles W Membreño, Janos Demeter, Roy Ng, Debadrita Bhattacharya, Thuyen N Nguyen, Griffin G Hartmann, Caterina I Colón, Carine Bossard, et al.Essential 8 medium (Thermo Fisher Scientific, A1517001) supplemented with 10 µM Rock inhibitor Y-27632 (Selleckchem, S1049). The next day, spheres were collected and transferred to 10 cm ultra-low-attachment dishes (Corning,

(16) The FAM53C/DYRK1A axis regulates the G1/S transition of the cell cycleElifeApril 30, 2026Taylar Hammond, Jong Bin Choi, Miles W Membreño, Janos Demeter, Roy Ng, Debadrita Bhattacharya, Thuyen N Nguyen, Griffin G Hartmann, Caterina I Colon, Carine Bossard, et al.Essential 8 medium (Thermo Fisher Scientific, A1517001) supplemented with 10 µM Rock inhibitor Y-27632 (Selleckchem, S1049). The next day, spheres were collected and transferred to 10 cm ultra-low-attachment dishes (Corning,

(17) Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal LungCurr ProtocApril 30, 2026Evelyn Tran, Laurence St. Pierre, Diego Velarde, Bianca Dal Bó, Juan Ramon Alvarez, Kweku Ofosu-Asante, Nazarius S. Lamango, Yong Huang, Crystal N. Marconett, Zea Borok, et al.100× antibiotic‐antimycotic (Gibco, 15240‐062)200 µl of 1 mM Y‐27632 (final 10 µM, Selleck Chemicals, S1049)Store up to 2 weeks at 4°C General freezing medium For 50 ml:45 ml filtered FBS (HyClone FBS, Thermo

(18) Deep Learning-Guided Holotomography Reveals Early Structural Remodelling During Pluripotency ExitbioRxivApril 27, 2026Hoewon Park, Geon Kim, Jeongwon Shin, Seo-Hyun Kim, Eui-been Hwang, Minji Kim, Taewoong Hwang, Arim Lim, Gyungtae Yoon, Jisu Park, et al.and transferred onto Matrigel-coated plates in the presence of ROCK inhibitor (Y27632; Selleckchem, #S1049). Reprogrammed iPSCs were adapted and maintained in E8 medium for more than 10 passages prior to imaging

(19) Emergent latent neurotoxic effects of manganese following nominal chronic exposures in human stem cell and Caenorhabditis elegans modelsToxicol SciApril 26, 2026Xueqi Tang, Airton C Martins, Anke M Tukker, Hyunjin Kim, Michael Aschner, Aaron B BowmanMatrigel-coated plates with mTeSR1 supplemented with 10 µM ROCK inhibitor Y-27632 (Selleckchem, Cat# S1049, dissolved in culture-graded water, stock concentration 10 mM). Once the hiPSC culture reached 100%

(20) Ciliated Cells Drive Critical STING-Mediated Tumor Suppression in the Fallopian Tube EpitheliumCancer ResApril 22, 2026Jose A. Colina, Maria Sol Recouvreux, Alexander M Sobeck, Benjamin K. Johnson, Yinzhi Lin, Sreeja C. Sekhar, Rita A. Avelar, Gabriela Rivera-Gonzalez, Yali Zhai, Harini Ram, et al.streptomycin (Invitrogen Cat #15140122) and freshly added: 10 μM ROCK inhibitor Y-27632 (Selleckchem Cat # S1049), wnt3a 100ng/ml (R&D systems Cat #5036-WN), β-estradiol 10nM. Secretory-only cultures were achieved

Be the First to Write a Review!