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Protein Biochemistry
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Coelenterazine-h
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Coelenterazine-h
Coelenterazine-h
Coelenterazine-h from Promega
(1)
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Citations:
(21)
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Promega for
Coelenterazine-h
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Description
We offer Coelenterazine (native) and Coelenterazine-h. Coelenterazine (native) is the luminescent substrate for Renilla luciferase and apoaequorin
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Coelenterazine-h
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
S2011
Quantity
250microg
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (21)
(1)
The incretin co-agonist tirzepatide requires GIPR for hormone secretion from human islets
Nature Metabolism
June 5, 2023
Kimberley El, Jonathan D. Douros, Francis S. Willard, Aaron Novikoff, Ashot Sargsyan, Diego Perez-Tilve, David B. Wainscott, Bin Yang, Alex Chen, Donald Wothe,
et al.
media was replaced with HBSS (cat. no.
14025092
;
Gibco
) containing 10 μM of coelenterazine-h (cat. no.
S2011
;
Promega
) or
1:500
dilution of NanoGlo (cat. no.
N1110
;
Promega
). BRET measurements were taken every
(2)
Expanding luciferase reporter systems for cell-free protein expression
Scientific Reports
December 1, 2022
Wakana Sato, Melanie Rasmussen, Christopher Deich, Aaron E. Engelhart, Katarzyna P. Adamala
(
Cayman Chemical Company
,
25836
), coelenterazine (
Cayman Chemical Company
,
16123
),
coelenterazine H
(
Promega
,
S2011
), frimazine (Aoblous, AOB36539), hispidin (
Cayman Chemical Company
,
10012605
), octanaldehyde (Fisher
(3)
Biased signaling due to oligomerization of the G protein-coupled platelet-activating factor receptor
Nature Communications
October 26, 2022
Junke Liu, Hengmin Tang, Chanjuan Xu, Shengnan Zhou, Xunying Zhu, Yuanyuan Li, Laurent Prézeau, Tao Xu, Jean-Philippe Pin, Philippe Rondard,
et al.
copper(II) (CuP, 362204) were purchased from
Sigma-Aldrich
(St. Louis, MO, USA).
Coelenterazine-h
(
S2011
), HaloTag® Alexa Fluor®660 (
G8471
), and HaloTag® Alexa Fluor® 488 (
G1001
) were purchased from
Promega Corporation
(4)
SERINC5 restricts influenza virus infectivity
PLoS pathogens
October 1, 2022
Zhao F, Xu F, Liu X, Hu Y, Wei L, Fan Z, Wang L, Huang Y, Mei S, Guo L,
et al
.
luciferase reporter IAV were collected and luciferase activity was measured using
Coelenterazine-h
(
Promega
,
S2011
) with a Luminometer (Modulus) following the manufacturer’s protocol. Silic acid linkage expression
(5)
GLP-1-mediated delivery of tesaglitazar improves obesity and glucose metabolism in male mice
Nature metabolism
August 1, 2022
Quarta C, Stemmer K, Novikoff A, Yang B, Klingelhuber F, Harger A, Bakhti M, Bastidas-Ponce A, Baugé E, Campbell JE,
et al
.
replaced with PBS (catalogue no. 10010056, Gibco) containing 10 μM of coelenterazine-h (catalogue no.
S2011
,
Promega
) or
1:500
dilution of NanoGlo (catalogue no.
N1110
,
Promega
). BRET measurements were taken
(6)
An anti-influenza A virus microbial metabolite acts by degrading viral endonuclease PA
Nature communications
April 19, 2022
Zhao J, Wang J, Pang X, Liu Z, Li Q, Yi D, Zhang Y, Fang X, Zhang T, Zhou R,
et al
.
by Reed and Muench13. Gluc activity
assay
Briefly, before
assay
, the coelenterazine-h (
Promega
, #
S2011
) was diluted to 16.7 μΜ with phosphate-buffered saline (PBS).
Cell culture
supernatants were added to
(7)
A peptide toxin in ant venom mimics vertebrate EGF-like hormones to cause long-lasting hypersensitivity in mammals
Proceedings of the National Academy of Sciences of the United States of America
February 15, 2022
Eagles DA, Saez NJ, Krishnarjuna B, Bradford JJ, Chin YK, Starobova H, Mueller A, Reichelt ME, Undheim EAB, Norton RS,
et al
.
Dulbecco’s phosphate-buffered saline (DPBS; Gibco; 2160) containing 5 µM coelenterazine-h (
Promega
;
S2011
). Light emissions at 430 to 485 nm (Rluc8) and 520 to 560 nm (Venus) were measured at 45-s intervals
(8)
Mutations that adapt SARS-CoV-2 to mink or ferret do not increase fitness in the human airway
Cell reports
February 8, 2022
Zhou J, Peacock TP, Brown JC, Goldhill DH, Elrefaey AME, Penrice-Randal R, Cowton VM, De Lorenzo G, Furnon W, Harvey WT,
et al
.
substrate,Europa Bioproducts,MO701A reporter lysis buffer,
Promega
,
E4030
Coelenterazine-H
,
Promega
,
S2011
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,, Ribomax T7
(9)
CD95/Fas suppresses NF-κB activation through recruitment of KPC2 in a CD95L/FasL-independent mechanism
iScience
December 17, 2021
Jean-Philippe Guégan, Justine Pollet, Christophe Ginestier, Emmanuelle Charafe-Jauffret, Marcus E. Peter, Patrick Legembre
pyrophosphatase,Sigma Aldrich,Cat.#I1643 Coomassie Brilliant Blue,,
Coelenterazine-h
,
Promega
,Cat.#
S2011
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,,
NucleoSpin RNA
(10)
An ionic lock and a hydrophobic zipper mediate the coupling between an insect pheromone receptor BmOR3 and downstream effectors
The Journal of biological chemistry
October 1, 2021
Lin JY, Yang Z, Yang C, Du JX, Yang F, Cheng J, Pan W, Zhang SJ, Yan X, Wang J,
et al
.
procedures
Materials The GloSensor cAMP
assay
reagent (
E1290
), Rluc substrate
Coelenterazine h
(
S2011
), and Nluc substrate furimazine (
N1661
) were purchased from
Promega
. TC-FlAsH II In-Cell Tetracysteine
(11)
Acetylation of calmodulin regulates synaptic plasticity and fear learning
The Journal of biological chemistry
September 1, 2021
Zhang HL, Zhao B, Han W, Sun YB, Yang P, Chen Y, Ni D, Zhang J, Yin DM.
(Sigma, H9395). We added 200 μl cell resuspension solution plus 50 μl luciferase substrate (
Promega
,
S2011
) in each well of 96-well plate and then read the emission ratio of 530 nm to 480 nm. The calcium concentration
(12)
Spatiotemporal GLP-1 and GIP receptor signaling and trafficking/recycling dynamics induced by selected receptor mono- and dual-agonists
Molecular metabolism
July 1, 2021
Novikoff A, O'Brien SL, Bernecker M, Grandl G, Kleinert M, Knerr PJ, Stemmer K, Klingenspor M, Zeigerer A, DiMarchi R,
et al
.
replaced with PBS (Cat #: 10010056, Gibco, Carlsbad, CA, USA) containing 10 μM of coelenterazine-h (Cat #:
S2011
,
Promega
, Madison, WI, USA) or
1:500
NanoGlo (Cat #:
N1110
,
Promega
, Madison, WI, USA). BRET1 measurements
(13)
Structure of a Hallucinogen-Activated Gq-Coupled 5-HT2A Serotonin Receptor
Cell
September 17, 2020
Kuglae Kim et al.
resin,Clontech,Cat#635507 1-Oleoyl-rac-glycerol (monoolein),Sigma,Cat#M7765
Cholesterol
,
Sigma
,Cat#C8667
Coelenterazine-h
,
Promega
,Cat#
S2011
Coelenterazine 400a (Deep Blue C),Nanolight,Cat#340-1 Poly-L-lysine,
Sigma
,Cat#P2636
Penicillin/Streptomycin
,
Invitrogen
,Cat#
15140
-122 Zeocin,
Invitrogen
,Cat#
R25005
Sf-900 II SFM
(14)
Distinct Binding Preferences between Ras and Raf Family Members and the Impact on Oncogenic Ras Signaling
Molecular Cell
December 1, 2019
Elizabeth M. Terrell et al.
S7842
PLX7904
,
SelleckChem
,Cat#
S7964
SB-590885,
SelleckChem
,Cat#
S2220
Coelenterazine-h
,
Promega
,Cat#
S2011
Halo Oregon Green ligand,
Promega
,Cat#
G2802
Epidermal Growth Factor (EGF),
ThermoFisher
,cat#
PHG0311
GST
-RBD,
Millipore
,cat#
(15)
Brodifacoum does not modulate human cannabinoid receptor-mediated hyperpolarization of AtT20 cells or inhibition of adenylyl cyclase in HEK 293 cells
PeerJ
September 25, 2019
Shivani Sachdev, Rochelle Boyd, Natasha L. Grimsey, Marina Santiago, Mark Connor
cAMP inhibition, all the drugs were made in 3 µM of forskolin.
Coelenterazine-h
substrate (2.5 µM) (#
S2011
;
Promega
, Madison, WI, USA) was added to the cells, and incubated for 5 mins prior to the addition of
(16)
VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells
Cell
July 25, 2019
English JG, Olsen RHJ, Lansu K, Patel M, White K, Cockrell AS, Singh D, Strachan RT, Wacker D, Roth BL.
followed by 30μL of drug (3X) per well, and finally 10μL of the RLuc substrate, coelenterazine h (
Promega
,
S2011
, 5 mM final concentration). Plates were incubated for 5 min to allow for substrate diffusion, and then
(17)
Limited AT1 Receptor Internalization Is a Novel Mechanism Underlying Sustained Vasoconstriction Induced by AT1 Receptor Autoantibody From Preeclampsia
Journal of the American Heart Association
March 19, 2019
Jingwei Bian, Jinghui Lei, Xiaochen Yin, Pengli Wang, Ye Wu, Xiaoli Yang, Li Wang, Suli Zhang, Huirong Liu, Michael L. X. Fu
between RLuc and YFP was measured after the addition of the RLuc substrate coelenterazine‐h (5 μmol/L,
S2011
,
Promega
, USA). The BRET signal was calculated as the ratio of emission of YFP (527 nm) to RLuc (370–480
(18)
Arrestin-biased AT1R agonism induces acute catecholamine secretion through TRPC3 coupling
Nature Communications
April 1, 2017
Chun-Hua Liu et al.
Subsequently, we incubated the
transfected
cells with
Coelenterazine h
at room temperature (
Promega
S2011
, final concentration, 5 μM) and two different light emissions were used for measurement (480/20 nm for
(19)
Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181
Molecular and Cellular Biology
March 1, 2016
Sarah M. Pedersen, Waipan Chan, Rakhi P. Jattani, deMauri S. Mackie, Joel L. Pomerantz
Gibco) and 90 l was aliquoted into white 96-well plates (
Costar
3912
).
Coelenterazine-h
(catalog number
S2011
;
Promega
) was added to the cells to a final concentration of 5 M, and BRET was measured 10 to 30 min
(20)
A protease-independent function for SPPL3 in NFAT activation
Molecular and cellular biology
January 1, 2015
Makowski SL, Wang Z, Pomerantz JL.
plates 10 to 40 min after the addition of coelenterazine-h to a final concentration of 5 M (catalog no.
S2011
;
Promega
). Rluc8 emission was detected over 1 s at 480 nm, and YFP emission was detected over 1 s at
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Reviews
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(1)
8/16/2022
Substrate Lights Up Experiments
Tobias Beigl
I was looking for a cheap Renilla luciferase substrate with a optimal price-to-performance-ratio and ended up using this substrate (Coelenterazine-h) in most of my luciferase assays. It works fine ...
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Coelenterazine-h from Promega
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