Coelenterazine-h from Promega

Product Specs
ItemCoelenterazine-h
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberS2011
Quantity250microg

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Development of pXeDNA3.1-LacZ, a Dual-System Plasmid for Membrane Protein Expression in Xenopus and Mammalian CellsMicroPubl BiolDecember 1, 2025Li Guan, Tom E. Reynoldson, Eleonora M. Pieroni, James C. Dillon, Iris Hardege, Luis A. Yanez-GuerramM NaHCO₃) without phenol and without FBS, supplemented with 4 µM coelenterazine H (Promega Cat. No. S2011). Cells were incubated in the dark at 37 °C for 2 h to allow aequorin loading. During this incubation,

(2) Identification of antimalarial drugs and bradykinin as ligands of the Plasmodium membrane protein PfSR10iScienceNovember 21, 2025Dan Jiang, Xin Wen, Ji-Fei Han, Qiu-Xia Cheng, Ru Zhang, Yuan Zheng, Kai Zheng, Shao-Hui Huang, Jia-Yuan Chen, Su-Wen Li, et al.synthesis des-Arg10-Kallidin,ChinaPeptides,Custom synthesis Melatonin,MCE,Cat# HY-B0075 Coelenterazine-h,Promega,Cat# S2011 Coelenterazine 400a,Maokangbio.,Cat# MX4610 FlAsH-EDT2,Thermo Fisher,Cat# T34561 2xClonExpression

(3) GABA-independent activation of GABAB receptor by mechanical forcesNature CommunicationsNovember 7, 2025Yujia Huo, Yiwei Zhou, Li Lin, Fan Yang, Jiyong Meng, Feiteng He, Fengfan Zhang, Mengdan Song, Cangsong Shen, Yuxuan Liu, et al.Baclofen (#0796) and Rac BHFF (#3313) were from Tocris Bioscience (Shanghai, China). Coelenterazine H (#S2011) was purchased from Promega (Beijing, China). Snap-Surface Alexa Fluor 647 (#S9136) was from New England Biolabs

(4) Spectral Tuning in Mammalian MelanopsinsMolecular Biology and EvolutionSeptember 25, 2025Richard J McDowell, Mazie J Gatt, Saba Notash, Robert J LucasInstitutes of Health). Prior to stimulation, cells were incubated with 10 µM Coelenterazine-h (Promega, Cat# S2011) in the dark for 2 h. Luminescence was recorded in a plate reader (Optima FLUOStar, BMG) modified with

(5) Encoding and decoding selectivity and promiscuity in the human chemokine-GPCR interaction networkCellJune 26, 2025Andrew B. Kleist, Martyna Szpakowska, Lindsay J. Talbot, Greg Slodkowicz, Duccio Malinverni, Monica A. Thomas, Kyler S. Crawford, Daniel J. McGrail, Acacia F. Dishman, Michael J. Wedemeyer, et al.,Molecular Devices,https://www.moleculardevices.com/products/assay-kits/gpcrs/flipr-calcium-assay-kits "HBSS, no Ca2+, no Mg2+",Life Technologies,14170112 Coelenterazine H,Promega,S2011 Nano-Glo live Cell substrate,Promega,https://www.promega.com/products/luciferase-assays/reporter-assays/nano-glo-extended-live-cellsubstrates/?catNum=N2570 ,, Critical

(6) A GLP-1 analogue optimized for cAMP-biased signaling improves weight loss in obese miceMolecular MetabolismMarch 27, 2025Jonathan D. Douros, Aaron Novikoff, Barent DuBois, Rebecca Rohlfs, Jacek Mokrosinski, Wouter F.J. Hogendorf, Robert Augustin, Myrte Merkestein, Lene Brandt Egaa Martini, Lars Linderoth, et al.replaced with PBS (Cat #: 10010056, Gibco, Carlsbad, CA, USA) containing 10 μM of coelenterazine-h (Cat #: S2011, Promega, Madison, WI, USA) or 1:500 NanoGlo (Cat #: N1110, Promega, Madison, WI, USA). BRET measurements

(7) High-affinity agonists reveal recognition motifs for the MRGPRD GPCRCell ReportsDecember 24, 2024Chunyu Wang, Yongfeng Liu, Marion Lanier, Adam Yeager, Isha Singh, Ryan H. Gumpper, Brian E. Krumm, Chelsea DeLeon, Shicheng Zhang, Marcus Boehm, et al.,Thermo Fisher Scientific,37069 Bright-Glo™ Luciferase Assay System,Promega,E2620 Coelenterazine h,Promega,S2011 Coelenterazine 400a,NanoLight Technology,Cat#340 AEBSF,GoldBio,Cat#A-540–5 Leupeptin,Sigma,Cat#L2884 E–64,AG

(8) Small-molecule GSDMD agonism in tumors stimulates antitumor immunity without toxicityCellOctober 31, 2024Pietro Fontana, Gang Du, Ying Zhang, Haiwei Zhang, Setu M. Vora, Jun Jacob Hu, Ming Shi, Ahmet B. Tufan, Liam B. Healy, Shiyu Xia, et al.710335C-25mg CELLview Cell Culture Dish,USA Scientific,Cat# 5662–7870 Coelenterazine-h,Promega,Cat# S2011 Collagenase D,Sigma,Cat# 11088866001 cOmplete™ Protease Inhibitor Cocktail,Sigma,Cat# 11836170001 DAPI,Thermo Fisher

(9) A pH-sensitive closed-loop nanomachine to control hyperexcitability at the single neuron levelNature communicationsJuly 4, 2024Merolla A, Michetti C, Moschetta M, Vacca F, Ciano L, Emionite L, Astigiano S, Romei A, Horenkamp S, Berglund K, et al.was diluted in ethanol and brought to a final concentration of 100 μM in saline solution. CTZh (cat. #S2011, Promega) was solubilized in 20 mM (2-Hydroxypropyl)-β-cyclodextrin in PBS as described73 and used at

(10) Large-scale deorphanization of Nematostella vectensis neuropeptide G protein-coupled receptors supports the independent expansion of bilaterian and cnidarian peptidergic systemsElifeMay 10, 2024Daniel Thiel, Luis Alfonso Yañez Guerra, Amanda Kieswetter, Alison G Cole, Liesbet Temmerman, Ulrich Technau, Gáspár Jékely5000,AAT Bioquest,60022,Used 0.3 µl per 100 ng of DNA "Chemical compound, drug",Coelenterazine-H,Promega,S2011,Diluted to 2 mM in ethanol and used at a final concentration of 4 µM in the assays "Chemical compound,

(11) Large-scale deorphanization of Nematostella vectensis neuropeptide G protein-coupled receptors supports the independent expansion of bilaterian and cnidarian peptidergic systemseLifeMay 10, 2024Daniel Thiel, Luis Alfonso Yañez Guerra, Amanda Kieswetter, Alison G Cole, Liesbet Temmerman, Ulrich Technau, Gáspár Jékely5000,AAT Bioquest,60022,Used 0.3 µl per 100 ng of DNA "Chemical compound, drug",Coelenterazine-H,Promega,S2011,Diluted to 2 mM in ethanol and used at a final concentration of 4 µM in the assays "Chemical compound,

(12) A Marine Natural Product, Harzianopyridone, as an Anti-ZIKV Agent by Targeting RNA-Dependent RNA PolymeraseMolecules (Basel, Switzerland)February 23, 2024Zhang K, Liang J, Zhang B, Huang L, Yu J, Xiao X, He Z, Tao H, Yuan J.opaque 96-well plate and injected with 60 μL of 16.7 μM coelenterazine-h (Promega, Madison, WI, USA, #S2011) per well. The luminescence was acquired for 2 s using the Infinite F500 (TECAN, Grödig, Austria). 4.9.

(13) Riboproteome remodeling during quiescence exit in Saccharomyces cerevisiaeiScienceJanuary 19, 2024Clara A. Solari, María Clara Ortolá Martínez, Juan M. Fernandez, Christian Bates, Gerardo Cueto, María Pía Valacco, Fabián Morales-Polanco, Silvia Moreno, Silvia Rossi, Mark P. Ashe, et al.TRIzol Reagent,Invitrogen,Cat#: 15596-026 Puromycin dihydrochloride,Sigma-Aldrich,Cat#: P9620 Coelenterazine-h,Promega,Cat#: S2011 Trypsin,Promega,Cat#: V5111 ,, Critical commercial assays,Critical commercial assays,Critical commercial

(14) The incretin co-agonist tirzepatide requires GIPR for hormone secretion from human isletsNature MetabolismJune 5, 2023Kimberley El, Jonathan D. Douros, Francis S. Willard, Aaron Novikoff, Ashot Sargsyan, Diego Perez-Tilve, David B. Wainscott, Bin Yang, Alex Chen, Donald Wothe, et al.media was replaced with HBSS (cat. no. 14025092; Gibco) containing 10 μM of coelenterazine-h (cat. no. S2011; Promega) or 1:500 dilution of NanoGlo (cat. no. N1110; Promega). BRET measurements were taken every

(15) Expanding luciferase reporter systems for cell-free protein expressionScientific ReportsDecember 1, 2022Wakana Sato, Melanie Rasmussen, Christopher Deich, Aaron E. Engelhart, Katarzyna P. Adamala(Cayman Chemical Company, 25836), coelenterazine (Cayman Chemical Company, 16123), coelenterazine H (Promega, S2011), frimazine (Aoblous, AOB36539), hispidin (Cayman Chemical Company, 10012605), octanaldehyde (Fisher

(16) RASopathy mutations provide functional insight into the BRAF cysteine-rich domain and reveal the importance of autoinhibition in BRAF regulationMolecular cellNovember 17, 2022Spencer-Smith R, Terrell EM, Insinna C, Agamasu C, Wagner ME, Ritt DA, Stauffer J, Stephen AG, Morrison DK.((Z)-4-Hydroxytamoxifen),Millipore Sigma,cat# H7904 Paraformaldehyde,EM Sciences,cat# 15710 Coelenterazine-h,Promega,cat# S2011 ,, Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays ,, CellTiter-Glo

(17) Biased signaling due to oligomerization of the G protein-coupled platelet-activating factor receptorNature communicationsOctober 26, 2022Liu J, Tang H, Xu C, Zhou S, Zhu X, Li Y, Prézeau L, Xu T, Pin JP, Rondard P, et al.copper(II) (CuP, 362204) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Coelenterazine-h (S2011), HaloTag® Alexa Fluor®660 (G8471), and HaloTag® Alexa Fluor® 488 (G1001) were purchased from Promega Corporation

(18) Signaling snapshots of 5-HT2B serotonin receptor activated by the prototypical psychedelic LSDNeuronOctober 5, 2022Can Cao, Ximena Barros-Álvarez, Shicheng Zhang, Kuglae Kim, Marc A. Dämgen, Ouliana Panova, Carl-Mikael Suomivuori, Jonathan F. Fay, Xiaofang Zhong, Brian E. Krumm, et al.Potassium Chloride,Sigma,Cat#793590 Magnesium Chloride,Sigma,Cat#M0250 TALON IMAC resin,Clontech,Cat#635507 Coelenterazine-h,Promega,Cat#S2011 Poly-L-lysine,Sigma,Cat#P2636 Penicillin/Streptomycin,Invitrogen,Cat#15140-122 Sf-900 II SFM,Invitrogen,Cat#10902096 ESF921,Expression

(19) SERINC5 restricts influenza virus infectivityPLoS pathogensOctober 1, 2022Zhao F, Xu F, Liu X, Hu Y, Wei L, Fan Z, Wang L, Huang Y, Mei S, Guo L, et al.luciferase reporter IAV were collected and luciferase activity was measured using Coelenterazine-h (Promega, S2011) with a Luminometer (Modulus) following the manufacturer’s protocol. Silic acid linkage expression

(20) GLP-1-mediated delivery of tesaglitazar improves obesity and glucose metabolism in male miceNature metabolismAugust 1, 2022Quarta C, Stemmer K, Novikoff A, Yang B, Klingelhuber F, Harger A, Bakhti M, Bastidas-Ponce A, Baugé E, Campbell JE, et al.replaced with PBS (catalogue no. 10010056, Gibco) containing 10 μM of coelenterazine-h (catalogue no. S2011, Promega) or 1:500 dilution of NanoGlo (catalogue no. N1110, Promega). BRET measurements were taken

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8/16/2022Substrate Lights Up Experiments Tobias BeiglI was looking for a cheap Renilla luciferase substrate with a optimal price-to-performance-ratio and ended up using this substrate (Coelenterazine-h) in most of my luciferase assays. It works fine ... Read Review