Description
DAF-2 is a sensitive fluorescent indicator commonly used for the detection of NO.{6360,6632} It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively.{6632} At neutral pH the detection limit for NO is 2-5 nM