DNase I Reaction Buffer from New England Biolabs

Product Specs
ItemDNase I Reaction Buffer
CompanyNew England Biolabs
Price $26.00 Inquire
Catalog NumberB0303S
Quantity6 ml
ApplicationsFunctional Genomics
TypeBuffer
Temperature-20 C

Specifications/Features

Storage:
,-20°C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) In vivo site-specific engineering to reprogram T cellsNatureMarch 18, 2026William A. Nyberg, Pierre-Louis Bernard, Wayne Ngo, Charlotte H. Wang, Jonathan Ark, Allison Rothrock, Gina M. Borgo, Gabriella R. Kimmerly, Jae Hyung Jung, Vincent Allain, et al.07820) gradient ultracentrifugation. AAV vector titres were determined by qPCR on DNase-I-treated (NEB, B0303S), proteinase-K-digested (Qiagen, 1114886) AAV samples after purification, using primers targeting the

(2) Capturing ribosomal structures in cellular extracts with cryoPRISM: A purification-free cryoEM approach reveals novel structural statesProceedings of the National Academy of SciencesMarch 3, 2026Mira B. May, Gabriella S. Lopez-Perez, Joseph H. Davistube with addition of 33 U/mL DNase I and 1X DNase I Reaction Buffer (New England Biolabs #M0303L and #B0303S, respectively), followed by a second spin at 21,000 g for 10 minutes. The resulting buffer composition

(3) Stepwise DNA unwinding gates TnpB genome-editing activitybioRxivJanuary 28, 2026Zehan Zhou, Iren Saffarian-Deemyad, Honglue Shi, Trevor Weiss, Muhammad Moez ur-Rehman, Kamakshi Vohra, Petr Skopintsev, Peter H. Yoon, Marena I. Trinidad, Conner J. Langeberg, et al.digestion was performed using RNase-free DNase I (NEB M0303S) with 0.5 μL 10× DNase I Reaction Buffer (NEB B0303S). Gel Filtration Buffer was added to each sample to a final volume of 5 μL. Reactions were incubated

(4) A Human Model of Oligodendrocyte Development Shows MCL-1 Influences Oligodendrocyte MorphogenesisGliaDecember 19, 2025Melanie Gil, Marina R. Hanna, Vivian Gamafollowing the Invitrogen TRIzol Reagent Protocol. RNA was incubated with DNase I (New England Biolabs, B0303S) at 37°C for 10 min. The reaction was inactivated by adding 0.1 M EDTA (EMD Millipore, 324506) and incubated

(5) DNA nanodevice for analysis of force-activated protein extension and interactionsNature NanotechnologyDecember 15, 2025Kun Zhou, Minhwan Chung, Shankar Pandey, Jing Cheng, John T. Powell, Qi Yan, Jun Liu, Yong Xiong, Martin A. Schwartz, Chenxiang LinU/μL, Thermo Fisher Scientific or New England Biolabs) in DNase I reaction buffer (New England Biolabs, B0303S) containing 0.1% Tween-20 at 37°C for 30 min while shaking. The samples were then analyzed by western blot

(6) Simulating cell-free chromatin using preclinical cancer models for liquid biopsy applicationsiScienceNovember 22, 2025Sasha C. Main, Steven D. De Michino, Lucas Penny, Aleem Aamir, Tina Keshavarzian, Benjamin H. Lok, Robert Kridel, David W. Cescon, Michael M. Hoffman, Mathieu Lupien, et al.25 μg/mL, and 50 μg/mL in a reaction mix consisting of 10% DNase1 buffer (New England Biolabs, CAT# B0303S), 1% BSA (New England Biolabs, CAT# B9000), and molecular-grade water. DNase1 was tested at 20 U/mL,

(7) Constrained reassortment and genotype-specific traits shape the evolutionary landscape of galbut virusVirus EvolutionNovember 4, 2025Alexandra H Keene-Snickers, Ali L Brehm, Tillie J Dunham, Taylor A Gelpi, Mark D Stengleinhigh-sensitivity RNA reagent (ThermoFisher, Q32852), and DNA was removed via a DNAse I treatment (NEB B0303S). Prior to library preparation, samples were normalized to 5 ng/μl except where sample concentrations

(8) Protocol optimization improves the performance of multiplexed RNA imagingScientific ReportsAugust 31, 2025Josh J. Luce, Christian A. Reardon-Lochbaum, Paolo Cadinu, Rosalind J. Xu, Jose Aceves-Salvador, Evangelia Semizoglou, Bertrand Wong, Iris Lopez, Alan B. Cantor, William Renthal, et al.protocol, samples were treated with 2000 units/µL DNase I (Thermo, EN0521) in 1× DNase buffer (NEB, B0303S) for 40 min at room temperature according to manufacturer’s instructions. Samples were washed once in

(9) Capturing ribosomal structures in cellular extracts with cryoPRISM: A purification-free cryoEM approach reveals novel structural statesBIORXIVAugust 22, 2025Mira B. May, Gabriella S. Lopez-Perez, Joseph H. Davistube with addition of 33 U/mL DNase I and DNase I Reaction Buffer (New England Biolabs #M0303L and #B0303S, respectively), followed by a second spin at 21,000g for 10 minutes. The resulting buffer composition

(10) Structural stabilization of the intrinsically disordered SARS-CoV-2 N by binding to RNA sequences engineered from the viral genome fragmentNat CommunJuly 15, 2025Sara Landeras-Bueno, Chitra Hariharan, Ruben Diaz Avalos, Andrew S. Norris, Dalton T. Snyder, Kathryn M. Hastie, Stephanie Harkins, Michelle Zandonatti, Roshan R. Rajamanickam, Eduardo Olmedillas, et al.inhibitors. The sample was incubated for 3 h at 37 °C. The template DNA was digested using 1 µl DNAse (NEB #B0303S) for 30 min at 37 °C. Then the RNA was purified using TRIzol and precipitated with isopropanol according

(11) A-to-I mRNA editing in bacteria can affect protein sequence, disulfide bond formation, and functionNucleic Acids ResJuly 2, 2025Liron Didi, Ofir Fargeon, Liam Aspit, Eyal Elias, Dor Braverman, Dganit Melamed, Daniel Keidar-Friedman, Nadav Sorek, Orit Raz, Sharon Ovnat Tamir, et al.shaker incubator at 200 rpm. Each sample was treated with 3 ml DNase solution: 10×DNaseI buffer (NEB, B0303S) and DNaseI 2000 units/ml (NEB, M0303L) according to protocol recommendation, 1 mM DTT, and 1 mM phenylmethylsulfonyl

(12) Flow cytometric analysis of the murine placenta to evaluate nanoparticle platforms during pregnancyPlacentaJune 13, 2025Kelsey L. Swingle, Alex G. Hamilton, Michael J. Mitchellwith 2 mL of 1× DNase I Reaction Buffer (diluted 10× in deionized water, New England BioLabs cat. #B0303S). Keep tubes on ice until further processing. Note 4: To increase statistical power, we recommend processing

(13) Cardiac-Specific Gene Editing via an AAV9-Tnnt2-SaCas9-miR122TS VectorBio-protocolMarch 5, 2025Yang L, Guo C, Sun Y, Guo Y.M0303S) 15. Proteinase K (Beyotime, catalog number: ST532) 16. 10× reaction buffer (NEB, catalog number: B0303S) For amplicon sequencing 1. TIANamp Genomic DNA kit (Tiangen, catalog number: GDP304-03) 2. TansNGC

(14) Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite productionNat CommunJanuary 21, 2025Duck Gyun Kim, Boncheol Gu, Yujin Cha, Jeonghan Ha, Yongjae Lee, Gahyeon Kim, Byung-Kwan Cho, Min-Kyu Ohreaction mixture containing 1 µg of total RNA, 1.5 µL of 10× DNase I Reaction Buffer (New England BioLabs, B0303S), 15 µL of hybridization buffer (90 mM Tris-HCl pH 7.5 and 200 mM KCl), 1 µL of oligo probe mixture

(15) Pathobiont-triggered induction of epithelial IDO1 drives regional susceptibility to Inflammatory Bowel DiseasebioRxiv : the preprint server for biologyJanuary 4, 2025Spencer PN, Wang J, Smith EP, Spiga L, Simmons AJ, Kim T, Kim W, Brown ME, Yang Y, Kaur H, et al.licheniformis,Sigma-Aldrich,P5380 Deoxyribonuclease (DNase) I from ovine pancreas,New England Biolabs,M0303S 1x DNase I buffer,New England Biolabs,B0303S DTT,Teknova,D9750 1X NP-40 Lysis High Salt Buffer Solution,RPI Corp.,N320000 100X Halt™ Protease

(16) MCL-1 regulates cellular transitions during oligodendrocyte developmentbioRxivDecember 23, 2024Melanie Gil, Marina R Hanna, Vivian Gamafollowing the Invitrogen TRIzol Reagent Protocol. RNA was incubated with DNase I (New England Biolabs, B0303S) at 37 °C for 10 minutes. Reaction was inactivated by adding 0.1 M EDTA (EMD Millipore, 324506) and

(17) DNA nanodevice for analysis of force-activated protein extension and interactionsbioRxivDecember 14, 2024Kun Zhou, Minhwan Chung, Jing Cheng, John T. Powell, Qi Yan, Jun Liu, Yong Xiong, Martin A. Schwartz, Chenxiang LinU/μL, Thermo Fisher Scientific or New England Biolabs) in DNase I reaction buffer (New England Biolabs, B0303S) containing 0.1% Tween-20 at 37°C for 30 min while shaking. The samples were then analyzed by western blot

(18) Early‐Branching Cyanobacteria Grow Faster and Upregulate Superoxide Dismutase Activity Under a Simulated Early Earth Anoxic AtmosphereGeobiologyDecember 12, 2024Sadia S. Tamanna, Joanne S. Boden, Kimberly M. Kaiser, Nicola Wannicke, Jonas Höring, Patricia Sánchez‐Baracaldo, Marcel Deponte, Nicole Frankenberg‐Dinkel, Michelle M. GehringerPCR was observed. A 10 μL volume of 10‐fold DNAse I reaction buffer (New England Biolabs, catalogue‐B0303S), 5 units (2.5 μL) of RNase free DNAse I (New England Biolabs, 2000 u. mL−1) and 100 μL of nuclease‐free

(19) RIPK2 Is Crucial for the Microglial Inflammatory Response to Bacterial Muramyl Dipeptide but Not to LipopolysaccharideInternational Journal of Molecular SciencesNovember 1, 2024Changjun Yang, Maria Carolina Machado da Silva, John Aaron Howell, Jonathan Larochelle, Lei Liu, Rachel E. Gunraj, Antônio Carlos Pinheiro de Oliveira, Eduardo Candelario-JalilWorthington Biochemical Corporation, Lakewood, NJ, USA) + 7.5 µL of 10X NEB DNase I Reaction Buffer (Cat. No. B0303S; New England BioLabs® Inc., Ipswich, MA, USA) was added to the silica resin bed of the column and incubated

(20) Brain-wide silencing of prion protein by AAV-mediated delivery of an engineered compact epigenetic editorScienceJune 28, 2024Edwin N. Neumann, Tessa M. Bertozzi, Elaine Wu, Fiona Serack, John W. Harvey, Pamela P. Brauer, Catherine P. Pirtle, Alissa Coffey, Michael Howard, Nikita Kamath, et al.of 1000U/mL Turbonuclease (Sigma T4330-50KU) with 1X DNase I reaction buffer (New England BioLabs, B0303S) in UltraPure DNase/RNase-Free distilled water at 37°C for one hour. Next, the endonuclease solution

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