DNase I Reaction Buffer from New England Biolabs

Product Specs
ItemDNase I Reaction Buffer
CompanyNew England Biolabs
Price $26.00 Inquire
Catalog NumberB0303S
Quantity6 ml
ApplicationsFunctional Genomics
TypeBuffer
Temperature-20 C

Specifications/Features

Storage:
,-20°C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The kinetoplastid-specific transcription factor mtTF1 is essential for mitochondrial gene expression and maxicircle replication in Trypanosoma bruceiPLoS BiolSeptember 21, 2026Bianca Manuela Berger, Enrico Santini, Himaja Manjunatha, Lea Trost, Markus Gerber, Hauke S. Hillen, Torsten Ochsenreiteraddition of 1 µl RNase R (NEB, # M0100S), 2.5 µl DNase I (NEB, # M0303S) and 22 µl DNase I buffer (NEB, #B0303S) according to the manufacturer protocol. Afterwards, samples were centrifuged at 14,000 rpm, 4 °C, for

(2) Micro- and nanoplastics remodel the autophagy-lysosomal axis and mitochondrial function in primary human monocytesFrontiers in ImmunologyAugust 26, 2026Stefania Pezzana, Martina Broggiato, Fiona Limanaqi, Elena Vezzoli, Silvia Zecchini, Luigia Valsecchi, Claudia Vanetti, Lara De Luca, Clara De Palma, Michela Bardini, et al.consisting of 0.25 μl of DNase I enzyme (M0303S, New England BioLabs) and 1.2 μl of 10× DNase buffer (B0303S, New England BioLabs), for a total volume of 1.45 μl. The reaction was then incubated at 37°C for 10

(3) TTC33 forms a complex with WDR61 and PHF5A to recruit factors relevant to genomic integrityNature CommunicationsAugust 21, 2026Rafał Tomecki, Małgorzata Drabko, Małgorzata Siek, Maria Matykiewicz, Miłosz Ludwinek, Łukasz S. Borowski, Aneta Jurkiewicz, Kamil Kobyłecki, Agnieszka Jabłońska, Dominik Cysewski, et al.of total RNA was subjected to DNase I (M0303S; New England BioLabs) treatment in dedicated buffer (B0303S; New England BioLabs) for 30 min. at 37 °C. RNA was next extracted briefly with acid phenol, cleared

(4) Stepwise DNA-unwinding gates TnpB genome-editing activityMolecular CellAugust 20, 2026Zehan Zhou, Iren Saffarian-Deemyad, Honglue Shi, Trevor Weiss, Muhammad Moez Ur-Rehman, Kamakshi Vohra, Petr Skopintsev, Peter H. Yoon, Marena I. Trinidad, Conner J. Langeberg, et al.DNase I (New England Biolabs, M0303S) with 0.5 μL 10× DNase I Reaction Buffer (New England Biolabs, B0303S). Gel Filtration Buffer was added to each sample to a final volume of 5 μL. Reactions were incubated

(5) Transcriptomic and Metabolomic Insights into Tissue- and Cultivar-Dependent Polysaccharide Accumulation in Anoectochilus roxburghiiInt J Mol SciAugust 1, 2026Xiaoqun Peng, Chao Liu, Yiping Liu, Haiwei Xie, Liangliang He, Yan Xie, Zhisheng Zhang, Menglong Wang, Shu ChenChina), RNase-free DNase I (M0303S, New England Biolabs, Ipswich, MA, USA), and DNase I Reaction Buffer (B0303S, New England Biolabs). Genomic DNA contamination was removed by DNase I treatment, and the extracted

(6) TCR origin and specificity to self or environmental antigens on different antigen presenting cells determine peripheral regulatory T cell differentiationImmunityJuly 28, 2026Xinxin Chi, Charlotte H. Wang, Yollanda Franco Parisotto, William A. Nyberg, Vanja Cabric, Adelaide Gelineau, Yi Cao, David L Owen, Jonas Ambjörnsson, Diane Mathis, et al.centrifugal filter units (Millipore Sigma #UFC910024). AAV titers were determined by qPCR using DNase I (NEB #B0303S)-treated and Proteinase K (Qiagen #1114886)-digested AAV samples. qPCR was performed with SsoFast Eva

(7) Production of virus-like particles with AsCas12a nuclease and CMV-driven crRNA for mammalian genome editingFrontiers in Genome EditingJune 29, 2026Natalia A. Kruglova, Sofiia E. Borovikova, Mikhail V. Shepelev0.2 µm PES filter and store at 4 °C.DNAse I (#M0303S, New England Biolabs, USA)10× DNAse I buffer (#B0303S, New England Biolabs, USA) 2.1.6 Flow cytometry PBSLabeled anti-CXCR4 antibody (#E-AB-F1157D, Elabscience,

(8) In vivo site-specific engineering to reprogram T cellsNatureMarch 18, 2026William A. Nyberg, Pierre-Louis Bernard, Wayne Ngo, Charlotte H. Wang, Jonathan Ark, Allison Rothrock, Gina M. Borgo, Gabriella R. Kimmerly, Jae Hyung Jung, Vincent Allain, et al.07820) gradient ultracentrifugation. AAV vector titres were determined by qPCR on DNase-I-treated (NEB, B0303S), proteinase-K-digested (Qiagen, 1114886) AAV samples after purification, using primers targeting the

(9) A robust cis -regulatory network ensures Otx2 expression during retinal developmentDevelopmentMarch 15, 2026Ian J. Purvis, Omar E. Ochoa Olmos, Ko Uoon Park, Michael L. Kaufman, Charles M. Henry, Chris Schaaf, Olivia J. Clise, Corey D. Tesdahl, Ami Haas, Joseph A. Brzezinskiwild-type cells, sorted cells were pelleted (5 min at 300 g) and incubated with 2 U of DNase (NEB, M0303S, B0303S) at 37°C for at least 30 min. Cells were washed three times with PBS before proceeding to genomic DNA

(10) Capturing ribosomal structures in cellular extracts with cryoPRISM: A purification-free cryoEM approach reveals novel structural statesProceedings of the National Academy of SciencesMarch 3, 2026Mira B. May, Gabriella S. Lopez-Perez, Joseph H. Davistube with addition of 33 U/mL DNase I and 1X DNase I Reaction Buffer (New England Biolabs #M0303L and #B0303S, respectively), followed by a second spin at 21,000 g for 10 minutes. The resulting buffer composition

(11) Stepwise DNA unwinding gates TnpB genome-editing activitybioRxivJanuary 28, 2026Zehan Zhou, Iren Saffarian-Deemyad, Honglue Shi, Trevor Weiss, Muhammad Moez ur-Rehman, Kamakshi Vohra, Petr Skopintsev, Peter H. Yoon, Marena I. Trinidad, Conner J. Langeberg, et al.digestion was performed using RNase-free DNase I (NEB M0303S) with 0.5 μL 10× DNase I Reaction Buffer (NEB B0303S). Gel Filtration Buffer was added to each sample to a final volume of 5 μL. Reactions were incubated

(12) A Human Model of Oligodendrocyte Development Shows MCL-1 Influences Oligodendrocyte MorphogenesisGliaDecember 19, 2025Melanie Gil, Marina R. Hanna, Vivian Gamafollowing the Invitrogen TRIzol Reagent Protocol. RNA was incubated with DNase I (New England Biolabs, B0303S) at 37°C for 10 min. The reaction was inactivated by adding 0.1 M EDTA (EMD Millipore, 324506) and incubated

(13) DNA nanodevice for analysis of force-activated protein extension and interactionsNature NanotechnologyDecember 15, 2025Kun Zhou, Minhwan Chung, Shankar Pandey, Jing Cheng, John T. Powell, Qi Yan, Jun Liu, Yong Xiong, Martin A. Schwartz, Chenxiang LinU/μL, Thermo Fisher Scientific or New England Biolabs) in DNase I reaction buffer (New England Biolabs, B0303S) containing 0.1% Tween-20 at 37°C for 30 min while shaking. The samples were then analyzed by western blot

(14) Simulating cell-free chromatin using preclinical cancer models for liquid biopsy applicationsiScienceNovember 22, 2025Sasha C. Main, Steven D. De Michino, Lucas Penny, Aleem Aamir, Tina Keshavarzian, Benjamin H. Lok, Robert Kridel, David W. Cescon, Michael M. Hoffman, Mathieu Lupien, et al.25 μg/mL, and 50 μg/mL in a reaction mix consisting of 10% DNase1 buffer (New England Biolabs, CAT# B0303S), 1% BSA (New England Biolabs, CAT# B9000), and molecular-grade water. DNase1 was tested at 20 U/mL,

(15) Constrained reassortment and genotype-specific traits shape the evolutionary landscape of galbut virusVirus EvolutionNovember 4, 2025Alexandra H Keene-Snickers, Ali L Brehm, Tillie J Dunham, Taylor A Gelpi, Mark D Stengleinhigh-sensitivity RNA reagent (ThermoFisher, Q32852), and DNA was removed via a DNAse I treatment (NEB B0303S). Prior to library preparation, samples were normalized to 5 ng/μl except where sample concentrations

(16) Protocol optimization improves the performance of multiplexed RNA imagingScientific ReportsAugust 31, 2025Josh J. Luce, Christian A. Reardon-Lochbaum, Paolo Cadinu, Rosalind J. Xu, Jose Aceves-Salvador, Evangelia Semizoglou, Bertrand Wong, Iris Lopez, Alan B. Cantor, William Renthal, et al.protocol, samples were treated with 2000 units/µL DNase I (Thermo, EN0521) in 1× DNase buffer (NEB, B0303S) for 40 min at room temperature according to manufacturer’s instructions. Samples were washed once in

(17) Capturing ribosomal structures in cellular extracts with cryoPRISM: A purification-free cryoEM approach reveals novel structural statesBIORXIVAugust 22, 2025Mira B. May, Gabriella S. Lopez-Perez, Joseph H. Davistube with addition of 33 U/mL DNase I and DNase I Reaction Buffer (New England Biolabs #M0303L and #B0303S, respectively), followed by a second spin at 21,000g for 10 minutes. The resulting buffer composition

(18) Structural stabilization of the intrinsically disordered SARS-CoV-2 N by binding to RNA sequences engineered from the viral genome fragmentNat CommunJuly 15, 2025Sara Landeras-Bueno, Chitra Hariharan, Ruben Diaz Avalos, Andrew S. Norris, Dalton T. Snyder, Kathryn M. Hastie, Stephanie Harkins, Michelle Zandonatti, Roshan R. Rajamanickam, Eduardo Olmedillas, et al.inhibitors. The sample was incubated for 3 h at 37 °C. The template DNA was digested using 1 µl DNAse (NEB #B0303S) for 30 min at 37 °C. Then the RNA was purified using TRIzol and precipitated with isopropanol according

(19) A-to-I mRNA editing in bacteria can affect protein sequence, disulfide bond formation, and functionNucleic Acids ResJuly 2, 2025Liron Didi, Ofir Fargeon, Liam Aspit, Eyal Elias, Dor Braverman, Dganit Melamed, Daniel Keidar-Friedman, Nadav Sorek, Orit Raz, Sharon Ovnat Tamir, et al.shaker incubator at 200 rpm. Each sample was treated with 3 ml DNase solution: 10×DNaseI buffer (NEB, B0303S) and DNaseI 2000 units/ml (NEB, M0303L) according to protocol recommendation, 1 mM DTT, and 1 mM phenylmethylsulfonyl

(20) Flow cytometric analysis of the murine placenta to evaluate nanoparticle platforms during pregnancyPlacentaJune 13, 2025Kelsey L. Swingle, Alex G. Hamilton, Michael J. Mitchellwith 2 mL of 1× DNase I Reaction Buffer (diluted 10× in deionized water, New England BioLabs cat. #B0303S). Keep tubes on ice until further processing. Note 4: To increase statistical power, we recommend processing

Be the First to Write a Review!