Y-27632 Dihydrochloride from Selleck Chemicals

Product Specs
ItemY-27632 Dihydrochloride
CompanySelleck Chemicals
Price $147.00Supplier Page View Company Product Page
Catalog NumberS1049
Quantity2mg
Purity99.86
TargetROCK
Storage3 years-20°C powder
FormulaC14H21N3O.2HCl

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Selleck Chemicals

14408 W Sylvanfield Drive

Houston, Texas TX 77014

United States

Phone: Toll Free: (877) 796-6397 (US and Canada only)
Tel: (832) 582-8158
Fax: (832) 582-8590

Email: sales@selleckchem.com

Company Profile

Website: www.selleckchem.com

(1) Microdystrophins partially rescue deficits of duchenne muscular dystrophy iPSC-cardiomyocytesMol Ther AdvSeptember 10, 2026Abiageal R. Keegan, Adriana Fernanda G.S. Gonzalez, Rongruo Zhang, Hung Doan, Sofía I. Torres-Bigio, Fiona Lau, Kaulen N. Ly, Elaine C. Lai, Leahlyn C. Mamuyac, Sriram Bhimaraju, et al.KnockOut Serum Replacement (KSR) (Invitrogen; 10828010), and 10 μM Y-27632 (Selleck Chemicals, Houston, TX; S1049). On days 18 and 19, iPSC-CMs were selected for resistance to zeocin 20 μg/mL (Fisher; R25001). From

(2) Establishment, biobanking, and multi-omics characterization of 41 patient-derived colorectal cancer organoids: MYC/PRC classificationTranslational OncologySeptember 1, 2026You Jin Lee, Jae Hyun Park, Hee Ju Nam, Soon-Chan Kim, Min Jung Kim, Seung-Yong Jeong, Ji Won Park, Ja-Lok KuCat#17101–015), hyaluronidase (20 µg mL⁻¹, Sigma-Aldrich, Cat#H3506), and Y-27632 (10 µM, Selleckchem, Cat#S1049). The cell suspension was filtered through a 100 µm strainer (SPL Life Sciences, Cat#93100), centrifuged

(3) E- and N-cadherin drive hepatic polarity and lumen elongation via opposing effects on RhoA activityJ Cell BiolAugust 3, 2026Junya Hayase, Li Yang, Yu-Heng Zhou, Kangji Wang, Cheng-Ran Xu, Erfei Biliver explants were cultured for 2 days in the presence of DMSO (D2650; Sigma-Aldrich) or 1 µM Y27632 (S1049; Selleck). Antibodies and chemicals Anti-E-cadherin (36/E-cadherin, 610182) and anti-N-cadherin (32/N-cadherin,

(4) Generation and characterization of two human induced pluripotent stem cell lines from myotonic dystrophy type 1 patientsStem Cell ResearchAugust 1, 2026Pooja Darji, Wenqiang Liu, Wenshu Zeng, Jade T. Chao, Paul D. Pang, Matthew T. Wheeler, Marco Perez, Joseph C. Wu70–80% confluency using 0.5 mM EDTA (Invitrogen #15575–038) with 10 μM ROCK inhibitor Y-27632 (Selleck #S1049) added for 24 h. The medium was replaced every 2 days. 4.3. Trilineage differentiation Passage 23

(5) Generation of two induced pluripotent stem cell lines from patients carrying heterozygous PTEN mutations associated with PTEN Hamartoma Tumor SyndromeStem Cell ResearchAugust 1, 2026Kristina Zdantsevich, Renke Tan, Dong Li, Parker Walther, Uri Ladabaum, Joseph C. Wu(Invitrogen, Cat #15575–038). After replating, 10 μM Y-27632, a ROCK inhibitor (Selleck Chemicals, Cat #S1049), was added for 24 h to enhance post-passaging survival. Cells were kept under standard stem cell culture

(6) Generation of two induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying MYBPC3 mutationsStem Cell ResearchAugust 1, 2026Catherine A. Wu, Matthew A. Wu, Shane R. Zhao, Junyi Sun, Amira G. Flores-Banuelos, Parker Walther, Matthew Wheeler, Nazish SayedXF medium was used to maintain the iPSCs. 10 μM of ROCK inhibitor (Y27632, Selleck Chemicals, Cat #S1049) was added to the medium during passaging. The medium was refreshed the next day and subsequently replenished

(7) B cell CD19 is transferred between immune cells in mice and humansNat CommunJuly 29, 2026Jasmin Ochs, Pia Schweineberg, Jacqueline Thode, Alica Blenkle, Leila Husseini, Matthias Klein, Tobias Bopp, Patrick Schindler, Friedemann Paul, Martin S. Weber#HZ-1045), 3 µM CHIR 99021 (Selleckchem; #S1263), and 10 µM ROCK inhibitor (Y-27632; Selleckchem; #S1049) onto Matrigel (Corning)-coated plates. After 18 h, medium was changed to TeSR-E6 (E6; Stemcell; #05946)

(8) KIF1A-mediated trafficking is required for neuronal autophagy in human neuronsbioRxivJuly 24, 2026Carris Borland, Jacob Popolow, Erika L. F. Holzbaur17502048 Non-essential Amino Acids (NEAA),Thermo Fisher Scientific,Cat# 11140050 ROCK Inhibitor Y-27632,Selleck,Cat# S1049 Revert™ 700 Total Protein Stain,LICOR,Cat#926-11021 NewBlot™ IR Stripping Buffer,LICOR,Cat# 928-40028 EveryBlot Blocking Buffer

(9) Expanded GEP-NET organoid culture for personalized therapy evaluationSci AdvJuly 24, 2026Steven D. Forsythe, Srujana V. Yellapragada, Tracey Pu, Darryl Nousome, Samarth Mathur, Dilara Akbulut, Stephen G. Andrews, Kamala A. Kenny, Martha Quezado, Margaret C. Cam, et al.growth factor–basic (1 ng/ml; 100-18B, PeproTech). During the first week of culture, 10 μM Y27632 (S1049, SelleckChem) was used and then removed from the following NET organoid formulations. To form organoids,

(10) IGF2BP3 is essential for the growth heterogeneity of colorectal adenoma cells by regulating MYCiScienceJuly 17, 2026Tomohiko Sunami, Roberto Coppo, Kunishige Onuma, Jumpei Kondo, Hiroyuki Uematsu, Daisuke Hoshi, Yoshihiro Yamamoto, Osamu Kikuchi, Shinya Ohashi, Yasuhide Takeuchi, et al.,Thermo Fisher Scientific,P3566 0.25% trypsin-EDTA,Thermo Fisher Scientific,25200072 Y-27632,Selleckchem,S1049 Noble agar,Sigma-Aldrich,A5431 ProLong™ Gold Antifade Mountant with DAPI,Thermo Fisher Scientific,P36931 Nutlin-3,ChemScene,CAS:548472-68-0 ,, Critical

(11) NOD1/2 signaling in macrophages drives adaptive immune resistance in cancerSignal Transduct Target TherJuly 16, 2026Xiduan Wei, Li Yang, Yuting Wang, Kun Wang, Dan Wang, Mengqian Gao, Xinhua Liu, Xuerui Yang, Suhua Wang, Yiran Zheng, et al.ng/mL human EGF (PeproTech; GMP100-15-100), 0.5 μM A83-01 (Selleck; S7692), and 10 μM Y-27632 (Selleck; S1049-10). Coculture system of PBMCs, MSS-CRC organoids, and tumor-reactive T cells Tumor-reactive T cell

(12) A feeder-free culture system supports long-term expansion and germline competence of bovine formative embryonic stem cells†Biology of ReproductionJuly 15, 2026Lei Chen, Furui Wang, Jiale He, Mingwei Sun, Yiru Wang, Yinjuan Wang, Shumin Wang, Lei An, Guangyin Xi, Jianhui Tianresuspended in DPBS containing 30% Matrigel (Corning, 354230) and 10 μM ROCK inhibitor Y-27632 (Selleck, S1049). A total of 5 × 106 cells in 100 μL were injected subcutaneously into the Inguinal flank of 8-week-old

(13) Protocol for establishing rectal tumor organoid-derived monolayers in a microphysiological system to model radiotherapySTAR ProtocJuly 15, 2026Eloïse Bouges, Charlotte Segers, Marco Lezzerini, Natalie Leys, Sarah Lebeer, Jianbo Zhang, Felice MastroleoSupplementa,ThermoFischer,CAT#175004044 Penicillin-Streptomycin,Sigma-Aldrich,CAT#P4333 Primocin,InvivoGen,CAT#2004131 Y-27632 2HCl,Selleckchem,CAT#S1049 Trypan blue 0.4%,Invitrogen,CAT#15250061 TrypLE Express,ThermoFischer,CAT#12604021 Cell recovery

(14) KDM8 acts as a co-regulator of transcription factor SOX2 for promoting cell pluripotencyStem Cell ReportsJuly 14, 2026Songqin Yang, Zhikai Ye, Lu Lin, Zhenlong Jiang, Erkang Wang, Jin Wangproteins","Chemicals, peptides, and recombinant proteins" ,, "ROCK inhibitor, Y27632",Selleck Chemicals,Cat# S1049 Camptothecin,MedChemExpress,Cat#HY-16560 ,, Critical commercial assays,Critical commercial assays,Critical

(15) Contractility Drives Cardiomyocyte Maturation and the Response to NanopatternsCirc ResJuly 9, 2026Laura A. Sherer, Abigail Nagle, Mary Papadaki, Seby Edassery, Tim McMillen, Dasom Yoo, Lauren D’Amico, Daniel Brambila-Diaz, Lucy Maynard, Mark Qiao, et al.a Matrigel-coated plate in mTeSR-1 media containing 10 μM Y-27632 ROCK Inhibitor (Selleck Chemicals S1049) at a density of 37,140 cells/cm2 in a 12-well plate. On the following day, the media was changed to

(16) Osimertinib-induced cardiotoxicity is driven by HDAC-dependent epigenetic repression and rescued by vorinostatSignal Transduct Target TherJuly 2, 2026Angelica Toro Cora, Arvind Singh Bhati, Allen Sam Titus, Ashish Jaiswal, Baldeep Singh, Prachi Umbarkar, Daniel Y. Li, Roshan Dutta, Qinkun Zhang, Suresh K. Verma, et al.(Stemcell Technologies 07920) and reseeded in MTeSR1 supplemented with 10 μM Y-27632, ROCK1 inhibitor (Selleckchem S1049) at a split ratio of 1:6. After 12 h, hiPSCs cells are switched to MTeSR1 without ROCK1 inhibitor. After

(17) CRISPR activation of DLX5 drives neural progenitors to the GnRH cell fateJ Mol EndocrinolJuly 1, 2026Shrinidhi Madhusudan, Nazli Eskici, Celia Gomez-Sanchez, Kristiina Pulli, Kirsi Vaaralahti, Venkatram Yellapragada, James R W Conway, Yafei Wang, Taneli Raivio200 U/mL collagenase IV (ThermoFisher Scientific, Cat. 17104019) and 10 μM Y-27632 (Selleckchem, Cat. S1049) in a 1:2 ratio and plated on Matrigel-coated dishes using basal N2B27 medium. From days 11 to 20, the

(18) DNA repair drives cisplatin-induced neuronal deathCellJune 25, 2026William J. Nathan, Chuanyuan Chen, Rosy Sakr, Bruno Siqueira Siqueira Mietto, Vincent van Batenburg, Jeroen van den Berg, Josette J. Wlaschin, Ferenc Livak, Elsa Callen, Nancy Wong, et al.(Thermofisher Scientific, cat. No. 35050061), 10 μM ROCK inhibitor (Y-27632; Selleckchem, cat. No. S1049), and 2 μg/mL doxycycline (Clontech, cat. No. 631311). N2 medium was removed and changed daily for two

(19) Identifying membrane-bound transcriptional regulatory proteins from rare but evolutionarily conserved domain combinationsNucleic Acids ResJune 25, 2026Muyoung Lee, Yeejin Jang, Qingqing Guo, Ujwal Punyamurtula, Joonhyuk Choi, Jonghwan Kim, Edward M Marcotte(Thermo Fisher Scientific, 10828028), 0.2 mM L-ascorbic acid (Wako, 013-12061), 2.5 µM Y27632 (Selleck Chemicals, S1049), 25 ng/ml EGF (PeproTech, AF-100-15), 0.8 mM VPA (Wako, 227-01071), 2 µM CHIR99021 (Selleck Chemicals,

(20) Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effectsCellJune 25, 2026Sriram Pendyala, Katie Partington, Nicholas Bradley, Abbye E. McEwen, Gwenneth Straub, Hyeon-Jin Kim, Shawn Fayer, Daniel Lee Holmes, Katherine A. Sitko, Riddhiman K. Garge, et al.until cells dissociated, then resuspended the cells in mTeSR Plus with 10 μM Y-27632 (SelleckChem, S1049) and replated. Media changes without re-plating used mTeSR Plus only. Cells were tested routinely for

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