ProLong Gold Antifade Reagent with DAPI from Cell Signaling Technology

Product Specs
ItemProLong Gold Antifade Reagent with DAPI
CompanyCell Signaling Technology
Price $268.00 Inquire
Catalog Number8961S
Quantity10 ml
Research AreaRelated Products
ApplicationsIF-P, IF-F, IF-IC

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Oridonin-Loaded PDA@Gel@GO Nanocapsules Modulate NLRP3 and Epithelial Repair in ColitisFASEB JApril 30, 2026Wenbo Yuan, Qirui Zhao, Jing Hua, Lingyao Xu, Yufan Wang, Tongtong Xu, Zuchao Du, Liping Wu, Daxiang CuiIgG (Abcam, ab150116, 1:500) were incubated for 1 h. Nuclei were stained with DAPI (Cell Signaling, #8961, 1:1000) for 5 min. After washing with PBS, imaging was performed using a confocal microscope (Leica

(2) RHOV is a Detachment-Responsive Rho GTPase Necessary for Ovarian Cancer Peritoneal MetastasisCancer ResApril 16, 2026Amal T. Elhaw, Priscilla W. Tang, Ya-Yun Cheng, Shriya Kamlapurkar, Zaineb Javed, Sarah Al-Saad, Sierra R. White, Ahmed Emam Abdelnaby, Hannah Khan, Alex Seok Choi, et al.coverslips were mounted using ProLong Gold Antifade Mountant with DAPI (Cell Signaling Technology, 8961) and cured overnight in the dark at room temperature. For wound healing experiments, cells were fixed

(3) Acute Seizure Susceptibility and Chronic Vascular Malformation in a Developmental Mouse Model of Sturge–Weber SyndromeInt J Mol SciApril 15, 2026Nicholas Truver, Chase Solomon, Anne Comi#A48265), and finally DAPI (ProLong Gold Antifade Reagent with DAPI, Cell Signaling, Danvers, MA, USA, #8961S). Primary antibodies were tested with negative controls in pilot studies to confirm specificity. Antigen

(4) Anti-PECAM-1 antibodies: another tool for visualization of Reissner´s fiber and the subcommissural organ in rat central nervous systemBrain Struct FunctApril 4, 2026Lukáš Malčický, Ján Košuth, Daniel Barčák, Jarmila Zrubáková, Marie Vancová, Zuzana Daxnerová, Juraj Ševc, Anna Alexovič Matiašováslides and cover-slipped using ProLong Gold Antifade Reagent with DAPI (Cell Signaling Technology, #8961). Samples were analyzed using the confocal system Leica TCS SP5 X equipped with LAS AF software (Leica

(5) ALKBH5‐Driven m6A Demethylation Boosts Inflammation and Autophagy in LPS‐Stimulated MacrophagesImmunity, Inflammation and DiseaseMarch 16, 2026Gui Wang, Ting Zhou, Shujun Zhouapplied and allowed to incubate. To visualize the nuclei, ProLong® Gold Antifade Reagent with DAPI (#8961, CST) was used. The cells were then imaged using fluorescence microscopy with z‐stack image reconstructions

(6) Primary cilia promote resistance to EGFR tyrosine kinase inhibitor, osimertinib, in non-small cell lung cancerbioRxivMarch 5, 2026Li Wang, Anuradha Pandit, Sk. Kayum Alam, Rhiannon Skauge, Sergio A. Gradilone, Luke H. Hoeppner1:200). Nuclei were stained with 4’, 6-diamino-2-phenylindole (DAPI; Cell Signaling Technology, Cat no. 8961). Alexa Fluor™ 488 goat anti-rabbit IgG (Invitrogen, A-11008) and Alexa Fluor™ 594 goat anti-mouse IgG

(7) Esophageal Cancer Cells Exhibit Heterogeneity in DNA Double-Strand Break Repair and G2/M Checkpoint Arrest Associated With Cell Viability After Ionizing RadiationAdvances in Radiation OncologyMarch 1, 2026Kohei Tateno, Ken Okuda, Shunji Haruna, Mayu Isono, Takaaki Ishikawa, Hikaru Okumura, Ryota Hayashi, Ryuta Suzuki, Takumi Takahashi, Tamaki Saito, et al.mounted on glass slides using ProLong Gold Antifade Reagent with 4,6-diamidino-2-phenylindole (DAPI) (#8961; Cell Signaling Technology). Antibodies The primary antibodies used in this study are listed in the

(8) Synapses and dendritic spines are eliminated in the primary visual cortex of mice subjected to chronic intraocular pressure elevationNeural Regeneration ResearchMarch 1, 2026Xinyi Zhang, Deling Li, Weiting Zeng, Yiru Huang, Zongyi Zhan, Yuning Zhang, Qinyuan Hu, Lianyan Huang, Minbin Yuwere mounted with the antifade reagent 4′,6-diamidino-2-phenylindole (Cell Signaling Technology, Cat# 8961). Imaging of glutamatergic and GABAergic synapse puncta was performed using a confocal laser scanning

(9) Inflamed vessel–anchored release of H 2 across the blood-brain barrier for ischemic stroke neuroprotectionScience AdvancesFebruary 27, 2026Yuanman Yu, Mingjian Fan, Gaoyi Wu, Yongcheng Li, Chao Xia, Wenjiang Ding, Guanglin Li, Qianjun He, Wei Tang, Changsheng Liusections were then mounted using ProLong Gold Antifade reagent with 4′,6-diamidino-2-phenylindole (CST, 8961S). In some experiments, Alexa Fluor 647–conjugated dextran (10 kDa, Invitrogen, D22914) was intravenously

(10) Uncovering the signatures of aging and senescence in the human dorsolateral prefrontal cortexCell GenomicsFebruary 11, 2026Nicholas X. Sloan, Jason Mares, Aidan C. Daly, Shaunice Grier, Imdadul Haq, Christopher A. Jackson, Natalie Barretto, Obadele Casel, Kristy Kang, Shruti Khiste, et al.(16%),Electron Microscopy Sciences,Cat# 15710 Prolong Gold Fluoromount with DAPI,Cell Signaling Technology,Cat# 8961S Quenching Buffer,Advanced Cell Diagnostics Inc.,Cat#BU08-L Recombinant Albumin,NEB,Cat# B9200S RNAse

(11) Generation of osteoclast-like cells from human peripheral blood mononuclear cells using NFATc1 modified RNAPLoS OneFebruary 11, 2026Sirada Srihirun, Chareerut Phruksaniyom, Thanaporn Sriwantana, Tipparat Parakaw, Nathawut Sibmooh, Pornpun Vivithanaporn, Kran Suknuntha(Abcam,#ab176753) for 1 hour and mouthed with prolong gold anti-fade DAPI to nuclei staining (Cell signaling,#8961). Images were randomly taken using the spiral scan path of the confocal microscope (Stellaris 5, Leica Microsystems

(12) Mangosteen (Garcinia mangostana) Pericarp and Leaf Tinctures Inhibit LPS-Induced Pro-Inflammatory Responses in Macrophages and Activate Nrf2NutrientsFebruary 5, 2026Restituto Tocmo, Mirielle C. Nauman, Yunying Huang, Pradeep Subedi, Jeremy James Johnsonfrom Atlanta Biologicals Inc. (Lawrenceville, GA, USA); and Prolong Gold Antifade with DAPI (Cat # 8961S) was purchased from CST. All other chemicals and solvents were of analytical grade and were purchased

(13) Multimodal single-cell network analysis uncovers BSG/CD147 as an early biomarker and signaling hub in hepatocellular carcinomaResearch SquareJanuary 29, 2026Thomas DW Wang, Eun-Young K Choi, Shuo Feng, Hui Jiang, Thomas DS Wang, Sangeeta Jaiswalwashed 3X with PBST and mounted with 1.5 μm coverslips using ProLong Gold Antifade Reagent with DAPI (8961; Cell Signaling Technologies). Fluorescence images were acquired using a confocal microscope with 20X

(14) Co‐Treatment With rhBMP‐2 and Rapamycin Modulates Osteogenesis–Adipogenesis Balance to Enhance Aged Bone RegenerationAdvanced ScienceJanuary 28, 2026Zirui He, Xiaoqiao Bai, Fangru Xie, Xuan Wang, Fan Zhang, Yuanman Yu, Yuan Yuan, Changsheng Liuanti‐rabbit immunoglobulin G (Alexa Fluro 488) were subsequently added, and then sealed with DAPI (CST, 8961). Images were captured using a laser confocal microscopy (Leica, TCS SP8, Germany). 5.19 Statistical

(15) Circulating adipocyte fatty acid-binding protein exacerbates LPS-induced neurotoxicity by crossing the disrupted blood–brain barrier and promoting neuronal apoptosisCell Communication and SignalingJanuary 23, 2026Muhammad Mustapha Ibrahim, Chunyan Li, Linhui Qiu, Yue Hu, Aimin Xu, Shilun Yang, Junlei Chang, Cheng Fangslides were mounted with ProLong Gold Antifade Reagent with DAPI (Cell Signaling Technology, Cat. #8961S). Fluorescence imaging was performed via a ZEISS Axio Imager Z2 microscope with ApoTome.2. Quantitative

(16) Assessment of the anticancer and antimetastatic effects of monocarbonyl analogs of curcumin, C66 and B2BrBC, in breast cancer cellsCancer Cell InternationalJanuary 21, 2026Radoslav Stojchevski, Sara Velichkovikj, Jane Bogdanov, Katerina Dragarska, Ivana Todorovska, Nikola Hadzi-Petrushev, Mitko Mladenov, Leonid Poretsky, Dimiter AvtanskiSlides were mounted using Prolong Gold Antifade Reagent with DAPI (Cell Signaling Technologies, Cat. # 8961). Cells were imaged at 100x magnification using EVOS FL Auto Imaging System (Thermo Fisher Scientific)

(17) Glycolysis inhibition functionally reprograms T follicular helper cells and reverses lupusCell ReportsDecember 23, 2025Seung Chul Choi, Yong Ge, Ahmed Elshikha, Yuk Pheel Park, Cenxiao Fang, Milind V. Joshi, Maria Montes Montes de Oca Arena, Lauren Padilla, Yanan Zhu, William L. Clapp, et al.Tech.,Cat#MD0110 Fixation/Permeabilization kit,eBioscience,Cat#00-5123-43 ProLong Gold Antifade Reagent with DAPI,Cell Signaling,Cat#8961 Autophagy Antibody Sampler Kit,Cell Signaling,Cat#3700S Cyto-ID Autophagy Detection Kit,Enzo Life

(18) R183Q GNAQ Sturge–Weber Syndrome Leptomeningeal and Cerebrovascular Developmental Mouse ModelJournal of Vascular AnomaliesDecember 19, 2025Chase Solomon, Meghan McCann, Pratibha Singh, Christina Nemeth, Anne ComiA48265), 1X PBS] and cover-slipped with ProLong® Gold Antifade Reagent with DAPI (Cell Signaling, #8961S). Microscopy and imaging: Stained slides were imaged using a Leica Thunder Imager at 5x, 20x, 40x,

(19) Cellular interactions in the sentinel lymph node predict melanoma recurrencebioRxivDecember 17, 2025Sabrina M. Solis, Yang Yang, Yee Hoon Foong, Anushka Dheer, Kevin L. Ma, Mohammad Saad Farooq, Shira G. Rosenberg, Sophie L. Gray-Gaillard, Giorgos C. Karakousis, Xiaowei Xu, et al.,Fisher Scientific,Cat# BP337-100 "ProLong® Gold Antifade Reagent, With DAPI",Cell Signaling Technology,Cat# 8961S CoverGrip™ Coverslip Sealant,Biotium,Cat# 23005 Nuclease Free Water (Molecular Grade H2O),Invitrogen,Cat#

(20) Targeting of the m6A eraser ALKBH5 suppresses stemness and chemoresistance of colorectal cancerNature CommunicationsDecember 13, 2025Heming Zhou, Huarong Chen, Weixin Liu, Cong Liang, Shiyan Wang, Kai Yuan, Alvin Ho Kwan Cheung, Yuet Wu, Wei Kang, Henley Cheung, et al.(1:500, Life Technologies). Then wash the slides by PBST for 3 times. Cover slip slides with DAPI (#8961, CST) and take images with Multiphoton Microscope (Leica TCS SP8). TUNEL assay TUNEL assay were conducted

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