FuGENE 6 Transfection Reagent from Promega

Product Specs
ItemFuGENE 6 Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2691
Quantity1ml
Transfection MethodNon-liposomal
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Direct activation of SARM1 by dsDNA is not supported by biochemical and cellular evidenceProc Natl Acad Sci U S ASeptember 15, 2026Allison H. Kao, Biswa P. Mishra, Mitchell Sorbello, Wenbin Zhang, Qinyi Zhou, Yuefeng Jiang, Veronika Masic, Paige Harten, Weixi Gu, Jian Yuan Yang, et al.and VSV-G or pMD2.G (Addgene #12259) using FuGENE 6 transfection reagent [Promega (Madison, WI); Cat.#E2691]. Viral supernatants were harvested at 48 h posttransfection, clarified by centrifugation (500×g, 10

(2) A Universal Plasticizer Alkylsulfonic Phenyl Ester Activates Pregnane X Receptor to Increase Intestinal Cholesterol UptakeInt J Mol SciSeptember 15, 2026Nnamdi Okore, Wangeci Kariuki, Conner Brown, Michael A. Moxley, Naara Ramirez, Yipeng Suithe corresponding luciferase reporter plasmids, together with β-gal control plasmids, using FuGENE 6 (E2691, Promega Corporation, Madison, WI, USA) in serum-free DMEM. For mammalian two-hybrid assays, HepG2 cells

(3) Lean adipocyte oxylipin signaling restrains breast cancer through ferroptosisScienceSeptember 10, 2026Meghan C. Curtin, Abigail E. Jackson, Mark D. Lee, Elisabeth A. Brown, J. Alan Maschek, David H. Lum, James E. Cox, Alana L. Welm, Keren I. Hilgendorf#8455), and pCMV-VSV-G (Addgene plasmid #8454) using OptiMEM and FuGENE 6 Transfection Reagent (Promega, E2691) according to manufacturer protocols. pCMV-dR8.2 dvpr and pCMV-VSV-G were a gift from Bob Weinberg.

(4) NAT10 is critical to block RNA sensing-induced IFN-β transactivation in viral infectionmBioSeptember 9, 2026Youngmin Park, Yan Liu, Jinshan He, Suha Eleya, Zhenyu Wu, Guillaume Nicolas Fiches, Dawei Zhou, Emily G. Watters, Zhixian M. He, Kateepe Arachchige Sudeera Nuwan Shanaka, et al.for 24 h. Cell transfection Poly(I:C) was transfected with the Fugene-6 transfection reagent (Cat. #E2691, Promega). Cells were incubated with Poly(I:C) for 24 h. For gene knockdown, siRNA (10 nM) was reversely

(5) Quantitative comparison of methodologies for translation site imaging in living cellsRNASeptember 1, 2026Agata D. Misiaszek, Esther Griesbach, Egle Jaugaite, Aurelio Ortale, Jan Eglinger, Tobias Hochstoeger, Jeffrey A. Chao(pHAGE lentiviral backbone) and accessory plasmids (VSV-G, Gag/Pol, Tat, Rev) using Fugene 6 (Promega E2691). The supernatant was harvested daily over 3 days, pooled and centrifuged, filtered through a 0.45 μm

(6) Astrocytes mobilize a broader repertoire of lysosomal repair mechanisms than neuronsiScienceAugust 21, 2026Erin M. Smith, Natali L. Chanaday, Sandra Madayplasmid expressing fluorescently-tagged proteins or 1 μg of plasmid to express shRNA. FuGENE6 (Promega, #E2691) reagent was used for the transfection following the manufacturer’s protocol. One day post transfection,

(7) Crosstalk between S-nitrosylation and glycation defines a metabolic vulnerability in liver and renal cancersNature CommunicationsAugust 20, 2026Chiara Pecorari, Mojca Bratina, Evelyn Gonzalez, Salvatore Rizza, Letizia Incampo, Lina Vardouli, Paola Giglio, Elena Maresca, Zsófia Márta Sztupinszki, Marcus Montanares Kildegaard Nielsen, et al.SHP002) and target plasmids (pLV-shAKR1A1 or pLV-shSCR) using Fugene® transfection reagent (Promega, E2691). After 16 h, the medium was replaced. Viral supernatants were collected at 24 h and again at 48 h post-transfection.

(8) Targeting NAT10 Activates Tumor-Intrinsic Immunity and Suppresses Tumor Progression in Head and Neck Squamous Cell CarcinomabioRxivAugust 8, 2026Thurbu Tshering Lepcha, Nithya Paruchuri, Dawei Zhou, Guillaume N. Fiches, Jinshan He, Kateepe Asn Shanaka, Yan Liu, Suha Eleya, Nischal Koirala, Jian Zhu, et al.STING agonist cyclic di-GMP (c-di-GMP) was transfected using FuGENE HD transfection reagent (Cat. #E2691, Promega). Western blotting Following transfection or treatment, cells were washed with cold PBS and

(9) FANCA-dependent FEN1 recruitment suppresses transcription-replication conflicts and PARPi sensitivityMolecular CellAugust 6, 2026Qinhong Wang, Simon W. Ellington, Paolo Guerra, Faeze Gharibpoor, Dennis A. Simpson, Min-Guk Cho, Adriana Beltran, Gaorav P. GuptaLipofectamine 3000 Transfection Reagent,Thermo Fisher Scientific,Cat# L3000008 FuGENE 6 Transfection Reagent,Promega,Cat# E2691 Lipofectamine RNAiMAX Reagent,Thermo Fisher Scientific,Cat# 13778030 Neon Transfection System 10 μl Kit

(10) Stable excitatory-inhibitory synapse balance despite dynamic turnoverElifeJuly 27, 2026Krassimira A Garbett, James P Allen, Jaybree M Lopez, Cassandra M Smith, Richard C Sando(pRSV-REV, pMDLg/pRRE, and vesicular stomatitis virus G protein [VSVG]) was done with FuGENE6 (Promega E2691) using 2.5 µg of each plasmid per 9.6 cm2. Lentiviral-containing medium was collected 48 hr after transfection,

(11) Cysteine Redox State Governs the Condensation Pathway of Hendra Virus W Protein and Differentially Impacts Type I IFN and NF-κB SignalingAdv Sci (Weinh)July 17, 2026Frank Gondelaud, Alexandre Lalande, Giulia Pesce, Auriane Bouchet, Carole Meunier, Christophe Bignon, Denis Ptchelkine, Yu Gu, Eva Ogire, Pierre-Yves Lozach, et al.pCAGGS vector or pCAGGS plasmids coding for tc‐tagged WHeV variants, using FuGENE 6 (Promega, Cat# E2691) according to the manufacturer's instructions, with a DNA:reagent ratio of 1:4. The medium was replaced

(12) High Throughput Characterization of Eukaryotic 2A-Like Peptides Identifies Novel Leucine-Associated Reduction in Protein AbundancebioRxivJuly 16, 2026John C. Snell, Kenneth A. Matreyekgrow for 24 hours, and then transfected using either xFect (Takara Bio, 631318) or Fugene 6 (Promega, E2691), with 100 ng of a vector containing eeBxb164 (Addgene plasmid A222339). After 24 hours, media was changed,

(13) The p.R249W Mutation in LMNA-Related Congenital Muscular Dystrophy Causes Nuclear Deformities and an Enrichment in Lamin A/C at the Ends of the NucleusCellsJuly 16, 2026Catherine Harvey, Zixuan Zhu, Iden Han, Kan Caolentivirus construct of interest, a pMDG.2 envelope vector, and a psPAX2 packaging vector using Fugene 6 (#E2691; Promega, Madison, WI, USA). The supernatant was collected 48 h and 72 h post-transfection and filtered

(14) Discovery of PIPE-791 , A Potent and Brain-Penetrant Lysophosphatidic Acid Receptor 1 Antagonist with Slow Tight Binding Characteristics for the Treatment of Neuroinflammatory DisordersJournal of Medicinal ChemistryJuly 9, 2026Austin Chen, Christopher Baccei, Jill Baccei, Alexander Broadhead, Kym I. Lorrain, Michael M. Poon, Jeffrey Roppe, Thomas O. Schrader, Karin J. Stebbins, Lino Valdez, et al.Diego, CA, USA). A transfection mix was prepared by adding Fugene 6 transfection mix (Promega, Cat. No. E2691) and 0.1 μg of the requisite plasmid to serum free media. HEK293T cells (ATCC, Cat. No. CRL-3216) were

(15) Dynamic Regulation of Endogenous Transcription Factor Hubs at Single-Molecule ResolutionAdv Sci (Weinh)July 8, 2026Shawn Yoshida, Yanghao Zhong, Akiko Kumagai, William G. Dunphy, Shasha Chongco‐transfected together with a SuperPiggyBac transposase vector into the A673 cell line using Fugene 6 (Promega, E2691) according to the manufacturer's instructions. 48 h after transfection, we started with selection by

(16) CDK1 and CEP97 cooperatively control centriole length to orchestrate ciliogenesis and developmental patterningGenes & DevelopmentJuly 1, 2026Yue Liu, Zhengmao Wang, Tanvi Sinha, Kathy H. Li, Robert J. Chalkley, Vicente Herranz-Pérez, Chang Xie, Bradley K. Yoder, Alma L. Burlingame, Brian L. Black, et al.Lenti-X 293T cells (HEK 293T; Takara) at 70%–80% confluence using Fugene 6 transfection reagent (Promega E2691). Medium containing lentiviral particles was filtered with a 0.45 µm PES membrane filter (Genesee Scientific

(17) Vaccination elicits HIV broadly neutralizing antibodies in primatesNatureJune 30, 2026Jon M. Steichen, Patrick J. Madden, Claudia T. Flynn, Swastik Phulera, Monolina Shil, Oleksandr Kalyuzhniy, Alessia Liguori, Carolyne Kifude, Leigh M. Sewall, Christopher A. Cottrell, et al.neutralization assay Pseudoviruses were produced in HEK293T cells (RRID: CVCL_0063) co-transfected using FuGENE 6 (Promega, E2691) or PEI Max (Polisciences, 24765-1) with pseudovirus Env-expressing plasmid and Env-deficient backbone

(18) Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activityiScienceJune 19, 2026Sven Kratochvil, Maximilian Kullmann, Henning Gruell, Sophie Sayettat, Chia-Hung Tsai, Natasa Vukovic, Christine Janaitis, Claudia Lindemann, Sandra Praßl, Ricarda Stumpf, et al.substrate",Biotrend Chemikalien GmbH,Cat#4380A CN54gp140,Polymun,#ENV002 FuGENE® 6 reagent,Promega,Cat#E2691 Phusion® Hot Start Flex DNA Polymerase,New England Biolabs,Cat#M0535L Maraviroc,NIH AIDS Reagent program,Cat#

(19) Receptor basis of unusual tissue tropism of avian influenza H5N1 clade 2.3.4.4b virus in cattleSci AdvJune 19, 2026Surabhi Srinivas, Shubhada K. Chothe, Santhamani Ramasamy, Sougat Misra, Noel Chandan Nallipogu, Lindsey LaBella, Yin-Ting Yeh, May Wang, Lauren E. Pepi, Heidi L. Pecoraro, et al.human embryonic kidney (HEK) 293T cells using Fugene6 transfection reagent (Promega, USA, catalog no. E2691). Pseudoviruses were collected 48 hours posttransfection, and titers were determined by infecting the

(20) Hemagglutinin double-mutation enhances binding of human-infecting avian influenza virus clade 2.3.4.4b H5Ny to human and SLeX receptorsEMBO ReportsJune 16, 2026Xiyue Jin, Pu Han, Yuxuan Wang, Haichen Wang, Chunge Zhang, Antonio Di Maio, Jin Yu, Tianjiao Hao, Yuhang Gu, Zeyu Zhang, et al.and other reagents" Insect-XPRESS,LONZA,Cat# 12-730Q FuGENE® 6 Transfection Reagent,Promega,Cat# E2691 Thrombin,Sigma,Cat# T4648 Goat Serum,Beyotime,Cat# C0265 "Maackia Amurensis Lectin I (MAL-I), Fluorescein",Vector Laboratories

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(1)

2/4/2020Best Transfection Reagent for Large-Sized DNA Sunita MeenaGood for large-sized DNA.
(1)

6/15/2016Fugene 6 Transfection Kit Benjamin GerovacThis kit is used to transfect mammalian cell lines. The kit is simple to use and results in high transfection efficiency.