FuGENE 6 Transfection Reagent from Promega

Product Specs
ItemFuGENE 6 Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2691
Quantity1ml
Transfection MethodNon-liposomal
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Multi-omic analysis reveals nitric oxide dependent remodeling in classically activated macrophages and identifies negative regulation mediated by AKR1A1Redox BiologyJune 1, 2026Nicholas L. Arp, Uzziah S. Urquiza, Marcel Morgenstern, Jonathan H. Schrope, James A. Votava, Steven V. John, Jack J. Stevens, Felicia Deng, Anna Huttenlocher, Joshua J. Coon, et al.lentiviral particles were produced by transfecting cells with FuGENE 6 Transfection Reagent (Promega, E2691) and Opti-MEM (Thermo Scientific, 31985070) containing psPAX2 packaging plasmid (Addgene, plasmid no.

(2) HCMV infection depends on EGLN1-mediated mitochondrial activation to increase dNTP pools for viral DNA replicationCell RepMay 26, 2026Lucas A. Simpson, Diana M. Dunn, Wyatt Fales, Zachary J. Moore, Jessica H. Ciesla, Nicole C. Waild, Matthew H. Raymonda, Isaac S. Harris, Joshua Munger#12259), and our lentiviral plasmid was incubated for 5 min. Following this incubation, FuGENE (Promega, E2691) was added to the mixture and incubated for 20 min. This solution was added to HEK 293T cells and incubated

(3) Hierarchical small molecule inhibition of MYST acetyltransferasesNat CommunMay 13, 2026Xuemin Chen, Alexandra Castroverde, Minervo Perez, Ronald Holewinski, Kiall F. Suazo, Rashmi Karki, Thorkell Andresson, Benjamin A. Garcia, Jordan L. Meier(4.4*10^5 cells/dish in 4.4 ml DMEM media). After 24 h, plasmids were transfected using Fugene 6 (Promega, E2691) according to the manufacturer’s instructions: 202.8 μL of OPTIM, 17.2 μL of Fugene 6 and 2.3 μg of

(4) Characterization and therapeutic suppression of KEAP1-NRF2-driven resistance to KRAS inhibitors in pancreatic and lung cancerbioRxivApril 24, 2026Wen-Hsuan Chang, Alec J. Vaughan, Addison G. Stamey, Mariana Mancini, Makiko Hayashi, Runying Yang, Ryan Robb, Daniel Andrussier, Jeffrey A. Klomp, Andrew M. Waters, et al.#12260, RRID: Addgene_12260), and pMD2.G (Addgene #12259, RRID: Addgene_12259) via FuGene 6 (Promega #E2691). HEK293T cells were incubated with the transfecting agents for 24 hours, and the medium was replaced

(5) ENPP1 buffers extracellular cGAMP in brown adipose tissue to limit insulin resistancebioRxivApril 17, 2026Songnan Wang, Yingjie Guo, Weidong An, Michelle Lee, Yu Li, Valentino Sudaryo, Gabriel Grenot, Gemini Skariah, Saranya C. Reghupaty, Sonny Young, et al.with MCS-13X Linker-BioID2-HA (empty vector, WT, or K173Q; Addgene 74224) using FuGENE 6 (Promega, #E2691) and Opti-MEM (Thermo Fischer, #31985088). At 24 h post-transfection, the culture media was supplemented

(6) The atypical adhesion GPCR ADGRA1 controls hippocampal inhibitory circuit functionCell ReportsApril 9, 2026Baris Tosun, Kelly Honkanen, Elizabeth Orput, Swarada Kulkarni, Duy Lan Huong Huong Bui, Richard C. Sando(pRSV-REV, pMDLg/pRRE and vesicular stomatitis virus G protein (VSVG)) was done with FuGENE6 (Promega E2691) using 2.5 μg of each plasmid per 9.6 cm2. Lentiviral-containing medium was collected 48 h after transfection,

(7) Lysosomal swelling triggers LRRK2 activitybioRxivApril 3, 2026Tuyana Malankhanova, Zhiyong Liu, Samuel Strader, Weiping Wang, Kiwoon Sung, Zhong Li, Shawn M. Ferguson, Andrew B. Westtransduction Lentiviral particles encoding TMEM192-3xHA were produced in 293FT cells using FuGENE 6 (Promega, Cat# E2691) transfection reagent. One day prior to transfection, 4–6 × 106 293FT cells were seeded into T75 flasks

(8) Radiation-induced autophagy regulates fibroblast mitochondrial metabolism and crosstalk with triple-negative breast cancer cellsCell ReportsMarch 24, 2026Kevin C. Corn, Shannon E. Martello, Vinay K. Menon, Lucy S. Britto, Kara M. Simmons, Youssef K. Mohamed, Yoanna I. Ivanova, Abtin A. Ghelmansaraei, Sara A. Weidenbach, Tian Zhu, et al.Following amplification, 1 μg of each plasmid was transfected into HEK293T cells using FuGene6 (Promega E2691) along with 0.75 μg packaging plasmid (psPAX2; AddGene 12260) and 0.25 μg envelope plasmid (pMD2.G;

(9) Four subtypes of disease-causing missense mutations underlie pathogenic protein interactions in neurodegenerative VPS13A diseaseJ Clin InvestMarch 24, 2026Xing Lin, Yuta Ryoden, Chigure Suzuki, Hiroyuki Ishikawa, Takaharu Sakuragi, Yasuo Uchiyama, Shigekazu Nagatacholesteryl hemisuccinate (CHS) was from Sigma-Aldrich (C6512). FuGENE 6 was purchased from Promega (E2691). Plasmids. pGag-pol-IRES-bsr (71) was a gift from T. Kitamura (Institute of Medical Science, the

(10) T cell development from expanded hematopoietic progenitors reveals initiation control by Lmo2 and Flt3L priming*Science ImmunologyMarch 13, 2026Boyoung Shin, Samantha J. Chang, Brendan W. MacNabb, Tom Sidwell, Brian A. Williams, Ellen V. Rothenbergor trio was pooled for packaging in Phoenix-Eco cells using Fugene 6 Transfection Reagent (Promega #E2691) according to the manufacturer’s protocols. The sequences of sgRNAs are in Supplemental Methods. Retroviral

(11) Palmitoylation Induced Activation of SMAD3 Exacerbates Colitis by Promoting Tissue-resident Memory T Cells DifferentiationInflammationMarch 4, 2026Yuejie Xu, Siqi Ji, Ping Jiang, Zhenguo Zhao, Qian Zhou, Dan Su, Shafi Ullah, Yu Zhao, Xiaoping Zou, Mingming Zhang, et al.verified by Western blotting. The transient transfection was carried out using either the FuGene 6 (E2691, Promega, Madison, WI, USA) (for HEK293T cells) or the Gene Pulser Xcell™ system (1652677, Bio-Rad,

(12) A Novel Heterozygous ARL3 Variant in Non-Syndromic Retinitis Pigmentosa: Clinical and Functional CharacterizationInt J Mol SciMarch 3, 2026Emilia Stellacci, Lucia Ziccardi, Alessandro Bruselles, Carmen Dell'Aquila, Luca Mignini, Marcello Niceta, Luigi Chiriatti, Mattia Carvetta, Erika Zara, Alessandro Leone, et al.transiently transfected with the wild-type and D67H, D67V, R99I, and T90C expression vectors Fugene 6 (E2691, Promega, Fitchburg, WI, USA), following the manufacturer’s instructions. Twenty-four hours after transfection,

(13) MRCKα Is a Suppressor of GEF-H1/RhoA/MRTF Signaling in Tubular CellsCellsMarch 2, 2026Veroni S. Sri Theivakadadcham, Qinghong Dan, Brian Wu, Shruthi Venugopal, Vida Maksimoska, Aysegul Yucel-Polat, Andras Kapus, Katalin Szásziwith 4–5 µg of GFP alone, or GFP-tagged GEF-H1 constructs using Fugene 6 transfection reagent (Cat# E2691, Promega Madison, WI, USA) following the manufacturer’s instructions. Cells were used 24 or 48 h post-transfection. For

(14) GLP ‐ 1R biased cAMP agonism maintains glycemic control with reduced malaise and emesis in preclinical mammalian modelsDiabetes, Obesity and MetabolismMarch 1, 2026Caitlin Baumer‐Harrison, Danya Aldaghma, Alex D. White, Sarah V. Applebey, Allison M. Pataro, Allaha Z. Mohiby, Brandon Alonso, Allison G. Xiao, Libbey S. O'Farrell, Yuewei Qian, et al.transiently transfected as previously described22 with Halo-tagged GLP1R constructs using Fugene-6 (Promega, E2691). After 48 h, transfected cells were spun down and resuspended in cAMP assay buffer (DMEM, Gibco 31053)

(15) Immune gene diversity and STING1 variants in shaping cancer immunity across different genetic ancestry populationsCell ReportsFebruary 24, 2026Xiaowen Hu, Jie Huang, Jiao Yuan, Yanrong Sun, Yuxin Wang, Zhongyi Hu, Junjie Jiang, Zhiling Wang, Bingwei Wang, Meixiao Long, et al.were transfected into the 293T packaging cell line using the FuGENE 6 Transfection Reagent (Cat No: E2691, Promega). The medium containing lentivirus was collected 48 hours post-transfection. SK-OV-3 and TOV-21G

(16) Structural and mechanistic analysis of covalent ligands targeting the RNA-binding protein NONOCell Chemical BiologyFebruary 19, 2026Garrett L. Lindsey, Thomas K. Hockley, Alejandro Villa Gomez, Andrew C. Marshall, William R. Brothers, Colin T. Finney, Jacob Gross, Archa H. Fox, Gene W. Yeo, Bruno Melillo, et al.MW 40,000",Polysciences,Cat#: 24765-1 RNAiMax,ThermoFisher,Cat#: 13778030 Fugene 6,Promega,Cat#: E2691 Polybrene,Santa Cruz,Cat#: 134220 Blasticidin,Fisher Scientific,Cat#: 50712728 Puromycin,Sigma-Aldrich,Cat#:

(17) Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stabilityMolecular CellFebruary 19, 2026Carlos Origel Marmolejo, Celina Sanchez, Erin Helms, Melissa J. McEvoy, Juyoung Lee, Marcel Werner, Paige Roberts, Stephan Hamperl, Joshua C. Saldivartransfected with pEGFP-H1.1 plasmid (Addgene, 32894) using FuGENE® 6 transfection reagent (Promega, E2691), following manufacturer’s guidelines. A transfection mixture was prepared by first adding FuGENE® 6 transfection reagent

(18) Structures of proteinase 3 and the CD177 receptor complex reveal a major autoantibody epitopeEMBO ReportsFebruary 17, 2026Céline Zheng-Gérard, Jana Joha, Maria Carrasquero, Kamel El Omari, Edward Lowe, Shirish Dubey, Simon J Draper, Yu-Chi Chang, Hsi-Hsien Lin, Alan D Salama, et al.from Jones Lab Grueninger-Leitch et al, 1996", Biotin,Sigma-Aldrich,Cat.#B4501 FuGENE 6,Promega,Cat.#E2691 Amine coupling kit,Cytiva,Cat.#BR100050 Nunc MaxiSorpTM flat-bottom plates,ThermoScientific,Cat.#735-0083 BlockerTM

(19) Multicenter international cohort study of haploinsufficiency of A20 reveals novel genetic architecture and phenotypic evolutionJournal of Allergy and Clinical ImmunologyFebruary 13, 2026Tingyan He, Jun Wang, Manuel Carpio Carpio Tumba, Shihao Wang, Youyou Luo, Jie Chen, Guomin Li, Zhou Shu, Song Zhang, Deborah L. Stone, et al.plasmids for GFP-tag TNFAIP3 wild type or mutants using Polyjet transfection reagents (Promega, Cat.No.E2691). After 48 hours, cells were left untreated or stimulated with TNF (20 ng/mL). Luciferase activity (the

(20) Env-antibody coevolution identifies B cell priming as the principal bottleneck to HIV V2 apex broadly neutralizing antibody developmentSci ImmunolFebruary 13, 2026Rumi Habib, Ryan S. Roark, Hui Li, Andrew Jesse Connell, Michael P. Hogarty, Kshitij Wagh, Shuyi Wang, Lorie Marchitto, Ashwin N. Skelly, John W. Carey, et al.proviral plasmid DNA was then transfected using FuGENE 6 transfection reagent (Promega, catalog no. E2691) according to the manufacturer’s instructions. Transfected cells were then incubated for 48 hours at

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(1)

2/4/2020Best Transfection Reagent for Large-Sized DNA Sunita MeenaGood for large-sized DNA.
(1)

6/15/2016Fugene 6 Transfection Kit Benjamin GerovacThis kit is used to transfect mammalian cell lines. The kit is simple to use and results in high transfection efficiency.