FuGENE 6 Transfection Reagent from Promega

Product Specs
ItemFuGENE 6 Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2691
Quantity1ml
Transfection MethodNon-liposomal
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Receptor basis of unusual tissue tropism of avian influenza H5N1 clade 2.3.4.4b virus in cattleSci AdvJune 19, 2026Surabhi Srinivas, Shubhada K. Chothe, Santhamani Ramasamy, Sougat Misra, Noel Chandan Nallipogu, Lindsey LaBella, Yin-Ting Yeh, May Wang, Lauren E. Pepi, Heidi L. Pecoraro, et al.human embryonic kidney (HEK) 293T cells using Fugene6 transfection reagent (Promega, USA, catalog no. E2691). Pseudoviruses were collected 48 hours posttransfection, and titers were determined by infecting the

(2) Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activityiScienceJune 19, 2026Sven Kratochvil, Maximilian Kullmann, Henning Gruell, Sophie Sayettat, Chia-Hung Tsai, Natasa Vukovic, Christine Janaitis, Claudia Lindemann, Sandra Praßl, Ricarda Stumpf, et al.substrate",Biotrend Chemikalien GmbH,Cat#4380A CN54gp140,Polymun,#ENV002 FuGENE® 6 reagent,Promega,Cat#E2691 Phusion® Hot Start Flex DNA Polymerase,New England Biolabs,Cat#M0535L Maraviroc,NIH AIDS Reagent program,Cat#

(3) Smoothened and ciliary GPCRs regulate ciliary protein kinase A activity involved in Hedgehog signal transductionPLoS BiolJune 10, 2026Thi D. Nguyen, Mia J. Konjikusic, Lorenzo M. Del Castillo, Roshanak Irannejad, Jeremy F. ReiterLenti-X 293T cells (Takara, 632180) at 70%–80% confluence using Fugene 6 transfection reagent (Promega, E2691). Medium containing lentiviral particles was collected 1 day after transfection and was concentrated

(4) Multi-omic analysis reveals nitric oxide dependent remodeling in classically activated macrophages and identifies negative regulation mediated by AKR1A1Redox BiologyJune 1, 2026Nicholas L. Arp, Uzziah S. Urquiza, Marcel Morgenstern, Jonathan H. Schrope, James A. Votava, Steven V. John, Jack J. Stevens, Felicia Deng, Anna Huttenlocher, Joshua J. Coon, et al.lentiviral particles were produced by transfecting cells with FuGENE 6 Transfection Reagent (Promega, E2691) and Opti-MEM (Thermo Scientific, 31985070) containing psPAX2 packaging plasmid (Addgene, plasmid no.

(5) Canonical microRNA loss drives tumor development, implicating therapeutic efficacy of enoxacin in angiosarcomaRNAJune 1, 2026Bozhi Liu, Ant Murphy, Annaleigh Benton, Lauren Gartenhaus, William Budka, Juliana M. Bronzini, Samuel Hartzler, Madison E. Yates, Alejandra Romero Alzate, Nimod D. Janson, et al.were treated with DMSO or 100 µM ENX. On the next day, cells were transfected with FuGENE6 (Promega E2691) with 5 ng of psiCHECK2 reporter plasmids (empty or a miR-497 sensor in which the Renilla luciferase

(6) A targetable developmental program co-regulates angiogenesis and immune evasion in melanomaCancer DiscovJune 1, 2026Pietro Berico, Amanda Flores Yanke, Fatemeh Vand-Rajabpour, Catherine Do, Irving Simonin Wilmer, Ines Delclaux, Tara Muijlwijk, Robert Stagnitta, Estefania Vazquez Cruz, Theodore Sakellaropoulos, et al.co-transfected with lentivector, psPAX2 (Addgene, 12260) and pMD2.G (Addgene, 12259) in Fugene6 (Promega, E2691). After 3 days of incubation, 293T supernatants were harvested, filtered and the virus concentrated

(7) HCMV infection depends on EGLN1-mediated mitochondrial activation to increase dNTP pools for viral DNA replicationCell RepMay 26, 2026Lucas A. Simpson, Diana M. Dunn, Wyatt Fales, Zachary J. Moore, Jessica H. Ciesla, Nicole C. Waild, Matthew H. Raymonda, Isaac S. Harris, Joshua Munger#12259), and our lentiviral plasmid was incubated for 5 min. Following this incubation, FuGENE (Promega, E2691) was added to the mixture and incubated for 20 min. This solution was added to HEK 293T cells and incubated

(8) SIRT2 mediates integrated stress response by deacetylating and stabilizing 4E-BP1 to suppress translationEMBO ReportsMay 18, 2026Yanlin Zi, Jiaqi Zhao, Miao Wang, Dan Hou, Richard A Cerione, Hening LinDual-luciferase Reporter assay system,Promega,E1910 TM,Synthesized in the lab,Methods section Polyethyleneimine,Polysciences,24765 FuGene6,Promega,E2691 CellTiter-Glo® 2.0 Cell Viability Assay,Promega,G9241 Acetyl-Lysine Affinity Beads,Cytoskeleton Inc,AAC04 MG132,MedChemExpress,HY-13259 Bafilomycin A1

(9) Hierarchical small molecule inhibition of MYST acetyltransferasesNat CommunMay 13, 2026Xuemin Chen, Alexandra Castroverde, Minervo Perez, Ronald Holewinski, Kiall F. Suazo, Rashmi Karki, Thorkell Andresson, Benjamin A. Garcia, Jordan L. Meier(4.4*10^5 cells/dish in 4.4 ml DMEM media). After 24 h, plasmids were transfected using Fugene 6 (Promega, E2691) according to the manufacturer’s instructions: 202.8 μL of OPTIM, 17.2 μL of Fugene 6 and 2.3 μg of

(10) Engineering Murine Cross‐Reactivity Into an Affibody to Human Death Receptor 5Biotechnology and BioengineeringMay 6, 2026Tse‐Han Kuo, Nagamani Vunnam, Jonathan N. Sachs, Benjamin J. Hackelal. 2020) in 1149 µL Opti‐MEM (Gibco 51985091) with 69.6 µL FuGENE 6 Transfection Reagent (Promega E2691). All HEK293 cells were cultured in a completed growth medium with DMEM (Gibco 11965092) supplemented

(11) Hepatitis B virus covalently closed circular DNA formation in murine hepatic cells uncovers a late entry blockProceedings of the National Academy of SciencesApril 28, 2026Xupeng Hong, Georgia Brousseau, Hsuan-An Chen, Catherine A. Freije, Leon L. Seifert, Mengyin Zhang, Yingpu Yu, William M. Schneider, Ype P. de Jong, Alan McLachlan, et al.Transfection Reagent (Roche, catalog no. 6366236001) or FuGENE6 transfection reagent (Promega, catalog no. E2691; for Huh7) at 3:1 reagent:DNA ratio. Stable cell line generation. To generate stable cell lines using

(12) Characterization and therapeutic suppression of KEAP1-NRF2-driven resistance to KRAS inhibitors in pancreatic and lung cancerbioRxivApril 24, 2026Wen-Hsuan Chang, Alec J. Vaughan, Addison G. Stamey, Mariana Mancini, Makiko Hayashi, Runying Yang, Ryan Robb, Daniel Andrussier, Jeffrey A. Klomp, Andrew M. Waters, et al.#12260, RRID: Addgene_12260), and pMD2.G (Addgene #12259, RRID: Addgene_12259) via FuGene 6 (Promega #E2691). HEK293T cells were incubated with the transfecting agents for 24 hours, and the medium was replaced

(13) ENPP1 buffers extracellular cGAMP in brown adipose tissue to limit insulin resistancebioRxivApril 17, 2026Songnan Wang, Yingjie Guo, Weidong An, Michelle Lee, Yu Li, Valentino Sudaryo, Gabriel Grenot, Gemini Skariah, Saranya C. Reghupaty, Sonny Young, et al.with MCS-13X Linker-BioID2-HA (empty vector, WT, or K173Q; Addgene 74224) using FuGENE 6 (Promega, #E2691) and Opti-MEM (Thermo Fischer, #31985088). At 24 h post-transfection, the culture media was supplemented

(14) The atypical adhesion GPCR ADGRA1 controls hippocampal inhibitory circuit functionCell ReportsApril 9, 2026Baris Tosun, Kelly Honkanen, Elizabeth Orput, Swarada Kulkarni, Duy Lan Huong Huong Bui, Richard C. Sando(pRSV-REV, pMDLg/pRRE and vesicular stomatitis virus G protein (VSVG)) was done with FuGENE6 (Promega E2691) using 2.5 μg of each plasmid per 9.6 cm2. Lentiviral-containing medium was collected 48 h after transfection,

(15) Lysosomal swelling triggers LRRK2 activitybioRxivApril 3, 2026Tuyana Malankhanova, Zhiyong Liu, Samuel Strader, Weiping Wang, Kiwoon Sung, Zhong Li, Shawn M. Ferguson, Andrew B. Westtransduction Lentiviral particles encoding TMEM192-3xHA were produced in 293FT cells using FuGENE 6 (Promega, Cat# E2691) transfection reagent. One day prior to transfection, 4–6 × 106 293FT cells were seeded into T75 flasks

(16) Radiation-induced autophagy regulates fibroblast mitochondrial metabolism and crosstalk with triple-negative breast cancer cellsCell ReportsMarch 24, 2026Kevin C. Corn, Shannon E. Martello, Vinay K. Menon, Lucy S. Britto, Kara M. Simmons, Youssef K. Mohamed, Yoanna I. Ivanova, Abtin A. Ghelmansaraei, Sara A. Weidenbach, Tian Zhu, et al.Following amplification, 1 μg of each plasmid was transfected into HEK293T cells using FuGene6 (Promega E2691) along with 0.75 μg packaging plasmid (psPAX2; AddGene 12260) and 0.25 μg envelope plasmid (pMD2.G;

(17) Four subtypes of disease-causing missense mutations underlie pathogenic protein interactions in neurodegenerative VPS13A diseaseJ Clin InvestMarch 24, 2026Xing Lin, Yuta Ryoden, Chigure Suzuki, Hiroyuki Ishikawa, Takaharu Sakuragi, Yasuo Uchiyama, Shigekazu Nagatacholesteryl hemisuccinate (CHS) was from Sigma-Aldrich (C6512). FuGENE 6 was purchased from Promega (E2691). Plasmids. pGag-pol-IRES-bsr (71) was a gift from T. Kitamura (Institute of Medical Science, the

(18) T cell development from expanded hematopoietic progenitors reveals initiation control by Lmo2 and Flt3L priming*Science ImmunologyMarch 13, 2026Boyoung Shin, Samantha J. Chang, Brendan W. MacNabb, Tom Sidwell, Brian A. Williams, Ellen V. Rothenbergor trio was pooled for packaging in Phoenix-Eco cells using Fugene 6 Transfection Reagent (Promega #E2691) according to the manufacturer’s protocols. The sequences of sgRNAs are in Supplemental Methods. Retroviral

(19) Palmitoylation Induced Activation of SMAD3 Exacerbates Colitis by Promoting Tissue-resident Memory T Cells DifferentiationInflammationMarch 4, 2026Yuejie Xu, Siqi Ji, Ping Jiang, Zhenguo Zhao, Qian Zhou, Dan Su, Shafi Ullah, Yu Zhao, Xiaoping Zou, Mingming Zhang, et al.verified by Western blotting. The transient transfection was carried out using either the FuGene 6 (E2691, Promega, Madison, WI, USA) (for HEK293T cells) or the Gene Pulser Xcell™ system (1652677, Bio-Rad,

(20) A Novel Heterozygous ARL3 Variant in Non-Syndromic Retinitis Pigmentosa: Clinical and Functional CharacterizationInt J Mol SciMarch 3, 2026Emilia Stellacci, Lucia Ziccardi, Alessandro Bruselles, Carmen Dell'Aquila, Luca Mignini, Marcello Niceta, Luigi Chiriatti, Mattia Carvetta, Erika Zara, Alessandro Leone, et al.transiently transfected with the wild-type and D67H, D67V, R99I, and T90C expression vectors Fugene 6 (E2691, Promega, Fitchburg, WI, USA), following the manufacturer’s instructions. Twenty-four hours after transfection,

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(1)

2/4/2020Best Transfection Reagent for Large-Sized DNA Sunita MeenaGood for large-sized DNA.
(1)

6/15/2016Fugene 6 Transfection Kit Benjamin GerovacThis kit is used to transfect mammalian cell lines. The kit is simple to use and results in high transfection efficiency.