FuGENE 6 Transfection Reagent from Promega

Product Specs
ItemFuGENE 6 Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2691
Quantity1ml
Transfection MethodNon-liposomal
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Radiation-induced autophagy regulates fibroblast mitochondrial metabolism and crosstalk with triple-negative breast cancer cellsCell ReportsMarch 24, 2026Kevin C. Corn, Shannon E. Martello, Vinay K. Menon, Lucy S. Britto, Kara M. Simmons, Youssef K. Mohamed, Yoanna I. Ivanova, Abtin A. Ghelmansaraei, Sara A. Weidenbach, Tian Zhu, et al.Following amplification, 1 μg of each plasmid was transfected into HEK293T cells using FuGene6 (Promega E2691) along with 0.75 μg packaging plasmid (psPAX2; AddGene 12260) and 0.25 μg envelope plasmid (pMD2.G;

(2) T cell development from expanded hematopoietic progenitors reveals initiation control by Lmo2 and Flt3L priming*Science ImmunologyMarch 13, 2026Boyoung Shin, Samantha J. Chang, Brendan W. MacNabb, Tom Sidwell, Brian A. Williams, Ellen V. Rothenbergor trio was pooled for packaging in Phoenix-Eco cells using Fugene 6 Transfection Reagent (Promega #E2691) according to the manufacturer’s protocols. The sequences of sgRNAs are in Supplemental Methods. Retroviral

(3) Palmitoylation Induced Activation of SMAD3 Exacerbates Colitis by Promoting Tissue-resident Memory T Cells DifferentiationInflammationMarch 4, 2026Yuejie Xu, Siqi Ji, Ping Jiang, Zhenguo Zhao, Qian Zhou, Dan Su, Shafi Ullah, Yu Zhao, Xiaoping Zou, Mingming Zhang, et al.verified by Western blotting. The transient transfection was carried out using either the FuGene 6 (E2691, Promega, Madison, WI, USA) (for HEK293T cells) or the Gene Pulser Xcell™ system (1652677, Bio-Rad,

(4) A Novel Heterozygous ARL3 Variant in Non-Syndromic Retinitis Pigmentosa: Clinical and Functional CharacterizationInt J Mol SciMarch 3, 2026Emilia Stellacci, Lucia Ziccardi, Alessandro Bruselles, Carmen Dell'Aquila, Luca Mignini, Marcello Niceta, Luigi Chiriatti, Mattia Carvetta, Erika Zara, Alessandro Leone, et al.transiently transfected with the wild-type and D67H, D67V, R99I, and T90C expression vectors Fugene 6 (E2691, Promega, Fitchburg, WI, USA), following the manufacturer’s instructions. Twenty-four hours after transfection,

(5) MRCKα Is a Suppressor of GEF-H1/RhoA/MRTF Signaling in Tubular CellsCellsMarch 2, 2026Veroni S. Sri Theivakadadcham, Qinghong Dan, Brian Wu, Shruthi Venugopal, Vida Maksimoska, Aysegul Yucel-Polat, Andras Kapus, Katalin Szásziwith 4–5 µg of GFP alone, or GFP-tagged GEF-H1 constructs using Fugene 6 transfection reagent (Cat# E2691, Promega Madison, WI, USA) following the manufacturer’s instructions. Cells were used 24 or 48 h post-transfection. For

(6) GLP ‐ 1R biased cAMP agonism maintains glycemic control with reduced malaise and emesis in preclinical mammalian modelsDiabetes, Obesity and MetabolismMarch 1, 2026Caitlin Baumer‐Harrison, Danya Aldaghma, Alex D. White, Sarah V. Applebey, Allison M. Pataro, Allaha Z. Mohiby, Brandon Alonso, Allison G. Xiao, Libbey S. O'Farrell, Yuewei Qian, et al.transiently transfected as previously described22 with Halo-tagged GLP1R constructs using Fugene-6 (Promega, E2691). After 48 h, transfected cells were spun down and resuspended in cAMP assay buffer (DMEM, Gibco 31053)

(7) Immune gene diversity and STING1 variants in shaping cancer immunity across different genetic ancestry populationsCell ReportsFebruary 24, 2026Xiaowen Hu, Jie Huang, Jiao Yuan, Yanrong Sun, Yuxin Wang, Zhongyi Hu, Junjie Jiang, Zhiling Wang, Bingwei Wang, Meixiao Long, et al.were transfected into the 293T packaging cell line using the FuGENE 6 Transfection Reagent (Cat No: E2691, Promega). The medium containing lentivirus was collected 48 hours post-transfection. SK-OV-3 and TOV-21G

(8) Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stabilityMolecular CellFebruary 19, 2026Carlos Origel Marmolejo, Celina Sanchez, Erin Helms, Melissa J. McEvoy, Juyoung Lee, Marcel Werner, Paige Roberts, Stephan Hamperl, Joshua C. Saldivartransfected with pEGFP-H1.1 plasmid (Addgene, 32894) using FuGENE® 6 transfection reagent (Promega, E2691), following manufacturer’s guidelines. A transfection mixture was prepared by first adding FuGENE® 6 transfection reagent

(9) Structural and mechanistic analysis of covalent ligands targeting the RNA-binding protein NONOCell Chemical BiologyFebruary 19, 2026Garrett L. Lindsey, Thomas K. Hockley, Alejandro Villa Gomez, Andrew C. Marshall, William R. Brothers, Colin T. Finney, Jacob Gross, Archa H. Fox, Gene W. Yeo, Bruno Melillo, et al.MW 40,000",Polysciences,Cat#: 24765-1 RNAiMax,ThermoFisher,Cat#: 13778030 Fugene 6,Promega,Cat#: E2691 Polybrene,Santa Cruz,Cat#: 134220 Blasticidin,Fisher Scientific,Cat#: 50712728 Puromycin,Sigma-Aldrich,Cat#:

(10) Structures of proteinase 3 and the CD177 receptor complex reveal a major autoantibody epitopeEMBO ReportsFebruary 17, 2026Céline Zheng-Gérard, Jana Joha, Maria Carrasquero, Kamel El Omari, Edward Lowe, Shirish Dubey, Simon J Draper, Yu-Chi Chang, Hsi-Hsien Lin, Alan D Salama, et al.from Jones Lab Grueninger-Leitch et al, 1996", Biotin,Sigma-Aldrich,Cat.#B4501 FuGENE 6,Promega,Cat.#E2691 Amine coupling kit,Cytiva,Cat.#BR100050 Nunc MaxiSorpTM flat-bottom plates,ThermoScientific,Cat.#735-0083 BlockerTM

(11) Env-antibody coevolution identifies B cell priming as the principal bottleneck to HIV V2 apex broadly neutralizing antibody developmentSci ImmunolFebruary 13, 2026Rumi Habib, Ryan S. Roark, Hui Li, Andrew Jesse Connell, Michael P. Hogarty, Kshitij Wagh, Shuyi Wang, Lorie Marchitto, Ashwin N. Skelly, John W. Carey, et al.proviral plasmid DNA was then transfected using FuGENE 6 transfection reagent (Promega, catalog no. E2691) according to the manufacturer’s instructions. Transfected cells were then incubated for 48 hours at

(12) Multicenter international cohort study of haploinsufficiency of A20 reveals novel genetic architecture and phenotypic evolutionJournal of Allergy and Clinical ImmunologyFebruary 13, 2026Tingyan He, Jun Wang, Manuel Carpio Carpio Tumba, Shihao Wang, Youyou Luo, Jie Chen, Guomin Li, Zhou Shu, Song Zhang, Deborah L. Stone, et al.plasmids for GFP-tag TNFAIP3 wild type or mutants using Polyjet transfection reagents (Promega, Cat.No.E2691). After 48 hours, cells were left untreated or stimulated with TNF (20 ng/mL). Luciferase activity (the

(13) Virus glycoprotein nanodisc platform for vaccine analyticsNature CommunicationsFebruary 10, 2026Kimmo Rantalainen, Alessia Liguori, Gabriel Ozorowski, Claudia Flynn, Jon M. Steichen, Olivia M. Swanson, Patrick J. Madden, Sabyasachi Baboo, Swastik Phulera, Anant Gharpure, et al.produced in human embryonic kidney 293 T cells (RRID:CVCL 0063) co-transfected using FuGENE 6 (Promega t#E2691) with pseudovirus Env-expressing plasmid and Env-deficient backbone plasmid (PSG3DEnv). PSVs were harvested

(14) Germ line LCP1 mutations cause immunodeficiency with neutropenia, monocytopenia, lymphopenia, and defective cytokinesis∗†Blood AdvancesFebruary 10, 2026Thijs van Bergen, Dennis A. Bosch, Christine Bellanné-Chantelot, Roger Mulet-Lazaro, Jacob R. Bledsoe, Lanpeng Chen, Hans W. J. de Looper, Claire van Dijk, Mariette N. D. ter Borg, Eric M. J. Bindels, et al.CATTTTACAAAGGACAATGC. These constructs were used to transfect HeLa cells using FuGENE 6 Transfection Reagent (Promega, E2691). Confocal microscopy of HeLa cells and primary B cells See the supplemental Methods for details. Results Clinical

(15) Microglial GM3 accumulation impairs Aβ phagocytic activity and promotes neuroinflammation in Alzheimer's diseaseNeurotherapeuticsFebruary 6, 2026Se Eun Park, Yun Jae Cha, Young-Kwang Kim, Hee Kyung Kim, Chaesun Kwon, Yoonah R. Oh, Geonmo Kim, Kristen Gah-Hyeon Kim, Hong-Gyun Lee, Yun Pyo Kang, et al.FBS and 1 % antibiotic-antimycotic. Plasmid transfection was performed using Fugene 6 (Promega, cat #E2691) for 24 h. Primary microglia were seeded in a 24-well plate at a density of 2 × 105 cells per well and

(16) Interferon regulatory factor 5 involves the pathogenesis of emphysema through NLRP3 and Ly6C expressing cellsExperimental & Molecular MedicineFebruary 5, 2026Sun-Hee Heo, Suk Young Park, Na Hyun Kim, Heeseo Kim, In-Jeoung Baek, Young Hoon Sung, Chiwook Chung, Sei Won LeeDNA per well was used for transfection with FuGene 6 transfection reagent (Promega, catalog number E2691) following the manufacturer’s instructions. The efficacy of overexpression of IRF5 was assessed by western blotting

(17) Repression of PRMT activities sensitize human homologous recombination-proficient ovarian and breast cancer cells to PARP inhibitor treatmenteLifeFebruary 3, 2026Youyou Zhang, Mu Xu, Jiao Yuan, Zhongyi Hu, Junjie Jiang, Yanrong Sun, Jie Huang, Yuxin Wang, Bingwei Wang, Jianfeng Shen, et al.,Thermo Fisher Scientific,Cat: #P11496,IF (1:1000) "Chemical compound, drug",FuGENE 6 Transfection Reagent,Promega,Cat: #E2691,

(18) Dynamic regulation of endogenous transcription factor hubs at single-molecule resolutionbioRxivJanuary 31, 2026Shawn Yoshida, Yanghao Zhong, Akiko Kumagai, William G. Dunphy, Shasha Chongco-transfected together with a SuperPiggyBac transposase vector into the A673 cell line using Fugene 6 (Promega, E2691) according to the manufacturer’s instructions. 48 hours after transfection, we started with selection

(19) PU.1 inhibition sensitizes stem-monocytic AML to BCL2 blockadebioRxivJanuary 28, 2026William M Yashar, Itallia V Pacentine, Akram Taherinasab, Thai Nguyen, Samantha Worme, Mitsuhiro Tsuchiya, Theresa Lusardi, Chelsea Hutchinson, Thomas L Fillmore, Camilo Posso, et al.packaging plasmid psPAX2 and envelope plasmid pMD2.G using FuGENE 6 Transfection Reagent (Promega #E2691). psPAX2 (Addgene plasmid #12260) and pMD2.G were gifts from Didier Trono (Addgene plasmid #12259).

(20) Granzyme B-mimetic nanozyme for nanovesicle targeted anticancer applicationsNature CommunicationsJanuary 26, 2026Xueyan Hu, Qiqi Liu, Helong Kang, Ming Zhao, Zheng Zhou, Yuanke Li, Weicheng Peng, Tianyi Qi, Ruming Liu, Li Jiao, et al.vesicular stomatitis virus G glycoprotein (VSVG) and cytomegalovirus (CMV) packaging plasmids using Fugene@6 (E2691, Promega) transfection reagent following the manufacturer’s instructions. The medium was changed after

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(1)

2/4/2020Best Transfection Reagent for Large-Sized DNA Sunita MeenaGood for large-sized DNA.
(1)

6/15/2016Fugene 6 Transfection Kit Benjamin GerovacThis kit is used to transfect mammalian cell lines. The kit is simple to use and results in high transfection efficiency.