FuGENE 6 Transfection Reagent from Promega

Product Specs
ItemFuGENE 6 Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2691
Quantity1ml
Transfection MethodNon-liposomal
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Stable excitatory-inhibitory synapse balance despite dynamic turnoverElifeJuly 27, 2026Krassimira A Garbett, James P Allen, Jaybree M Lopez, Cassandra M Smith, Richard C Sando(pRSV-REV, pMDLg/pRRE, and vesicular stomatitis virus G protein [VSVG]) was done with FuGENE6 (Promega E2691) using 2.5 µg of each plasmid per 9.6 cm2. Lentiviral-containing medium was collected 48 hr after transfection,

(2) Cysteine Redox State Governs the Condensation Pathway of Hendra Virus W Protein and Differentially Impacts Type I IFN and NF-κB SignalingAdv Sci (Weinh)July 17, 2026Frank Gondelaud, Alexandre Lalande, Giulia Pesce, Auriane Bouchet, Carole Meunier, Christophe Bignon, Denis Ptchelkine, Yu Gu, Eva Ogire, Pierre-Yves Lozach, et al.pCAGGS vector or pCAGGS plasmids coding for tc‐tagged WHeV variants, using FuGENE 6 (Promega, Cat# E2691) according to the manufacturer's instructions, with a DNA:reagent ratio of 1:4. The medium was replaced

(3) High Throughput Characterization of Eukaryotic 2A-Like Peptides Identifies Novel Leucine-Associated Reduction in Protein AbundancebioRxivJuly 16, 2026John C. Snell, Kenneth A. Matreyekgrow for 24 hours, and then transfected using either xFect (Takara Bio, 631318) or Fugene 6 (Promega, E2691), with 100 ng of a vector containing eeBxb164 (Addgene plasmid A222339). After 24 hours, media was changed,

(4) The p.R249W Mutation in LMNA-Related Congenital Muscular Dystrophy Causes Nuclear Deformities and an Enrichment in Lamin A/C at the Ends of the NucleusCellsJuly 16, 2026Catherine Harvey, Zixuan Zhu, Iden Han, Kan Caolentivirus construct of interest, a pMDG.2 envelope vector, and a psPAX2 packaging vector using Fugene 6 (#E2691; Promega, Madison, WI, USA). The supernatant was collected 48 h and 72 h post-transfection and filtered

(5) Discovery of PIPE-791 , A Potent and Brain-Penetrant Lysophosphatidic Acid Receptor 1 Antagonist with Slow Tight Binding Characteristics for the Treatment of Neuroinflammatory DisordersJournal of Medicinal ChemistryJuly 9, 2026Austin Chen, Christopher Baccei, Jill Baccei, Alexander Broadhead, Kym I. Lorrain, Michael M. Poon, Jeffrey Roppe, Thomas O. Schrader, Karin J. Stebbins, Lino Valdez, et al.Diego, CA, USA). A transfection mix was prepared by adding Fugene 6 transfection mix (Promega, Cat. No. E2691) and 0.1 μg of the requisite plasmid to serum free media. HEK293T cells (ATCC, Cat. No. CRL-3216) were

(6) Dynamic Regulation of Endogenous Transcription Factor Hubs at Single-Molecule ResolutionAdv Sci (Weinh)July 8, 2026Shawn Yoshida, Yanghao Zhong, Akiko Kumagai, William G. Dunphy, Shasha Chongco‐transfected together with a SuperPiggyBac transposase vector into the A673 cell line using Fugene 6 (Promega, E2691) according to the manufacturer's instructions. 48 h after transfection, we started with selection by

(7) CDK1 and CEP97 cooperatively control centriole length to orchestrate ciliogenesis and developmental patterningGenes & DevelopmentJuly 1, 2026Yue Liu, Zhengmao Wang, Tanvi Sinha, Kathy H. Li, Robert J. Chalkley, Vicente Herranz-Pérez, Chang Xie, Bradley K. Yoder, Alma L. Burlingame, Brian L. Black, et al.Lenti-X 293T cells (HEK 293T; Takara) at 70%–80% confluence using Fugene 6 transfection reagent (Promega E2691). Medium containing lentiviral particles was filtered with a 0.45 µm PES membrane filter (Genesee Scientific

(8) Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activityiScienceJune 19, 2026Sven Kratochvil, Maximilian Kullmann, Henning Gruell, Sophie Sayettat, Chia-Hung Tsai, Natasa Vukovic, Christine Janaitis, Claudia Lindemann, Sandra Praßl, Ricarda Stumpf, et al.substrate",Biotrend Chemikalien GmbH,Cat#4380A CN54gp140,Polymun,#ENV002 FuGENE® 6 reagent,Promega,Cat#E2691 Phusion® Hot Start Flex DNA Polymerase,New England Biolabs,Cat#M0535L Maraviroc,NIH AIDS Reagent program,Cat#

(9) Receptor basis of unusual tissue tropism of avian influenza H5N1 clade 2.3.4.4b virus in cattleSci AdvJune 19, 2026Surabhi Srinivas, Shubhada K. Chothe, Santhamani Ramasamy, Sougat Misra, Noel Chandan Nallipogu, Lindsey LaBella, Yin-Ting Yeh, May Wang, Lauren E. Pepi, Heidi L. Pecoraro, et al.human embryonic kidney (HEK) 293T cells using Fugene6 transfection reagent (Promega, USA, catalog no. E2691). Pseudoviruses were collected 48 hours posttransfection, and titers were determined by infecting the

(10) Hemagglutinin double-mutation enhances binding of human-infecting avian influenza virus clade 2.3.4.4b H5Ny to human and SLeX receptorsEMBO ReportsJune 16, 2026Xiyue Jin, Pu Han, Yuxuan Wang, Haichen Wang, Chunge Zhang, Antonio Di Maio, Jin Yu, Tianjiao Hao, Yuhang Gu, Zeyu Zhang, et al.and other reagents" Insect-XPRESS,LONZA,Cat# 12-730Q FuGENE® 6 Transfection Reagent,Promega,Cat# E2691 Thrombin,Sigma,Cat# T4648 Goat Serum,Beyotime,Cat# C0265 "Maackia Amurensis Lectin I (MAL-I), Fluorescein",Vector Laboratories

(11) Smoothened and ciliary GPCRs regulate ciliary protein kinase A activity involved in Hedgehog signal transductionPLoS BiolJune 10, 2026Thi D. Nguyen, Mia J. Konjikusic, Lorenzo M. Del Castillo, Roshanak Irannejad, Jeremy F. ReiterLenti-X 293T cells (Takara, 632180) at 70%–80% confluence using Fugene 6 transfection reagent (Promega, E2691). Medium containing lentiviral particles was collected 1 day after transfection and was concentrated

(12) A targetable developmental program co-regulates angiogenesis and immune evasion in melanomaCancer DiscovJune 1, 2026Pietro Berico, Amanda Flores Yanke, Fatemeh Vand-Rajabpour, Catherine Do, Irving Simonin Wilmer, Ines Delclaux, Tara Muijlwijk, Robert Stagnitta, Estefania Vazquez Cruz, Theodore Sakellaropoulos, et al.co-transfected with lentivector, psPAX2 (Addgene, 12260) and pMD2.G (Addgene, 12259) in Fugene6 (Promega, E2691). After 3 days of incubation, 293T supernatants were harvested, filtered and the virus concentrated

(13) Multi-omic analysis reveals nitric oxide dependent remodeling in classically activated macrophages and identifies negative regulation mediated by AKR1A1Redox BiologyJune 1, 2026Nicholas L. Arp, Uzziah S. Urquiza, Marcel Morgenstern, Jonathan H. Schrope, James A. Votava, Steven V. John, Jack J. Stevens, Felicia Deng, Anna Huttenlocher, Joshua J. Coon, et al.lentiviral particles were produced by transfecting cells with FuGENE 6 Transfection Reagent (Promega, E2691) and Opti-MEM (Thermo Scientific, 31985070) containing psPAX2 packaging plasmid (Addgene, plasmid no.

(14) Canonical microRNA loss drives tumor development, implicating therapeutic efficacy of enoxacin in angiosarcomaRNAJune 1, 2026Bozhi Liu, Ant Murphy, Annaleigh Benton, Lauren Gartenhaus, William Budka, Juliana M. Bronzini, Samuel Hartzler, Madison E. Yates, Alejandra Romero Alzate, Nimod D. Janson, et al.were treated with DMSO or 100 µM ENX. On the next day, cells were transfected with FuGENE6 (Promega E2691) with 5 ng of psiCHECK2 reporter plasmids (empty or a miR-497 sensor in which the Renilla luciferase

(15) HCMV infection depends on EGLN1-mediated mitochondrial activation to increase dNTP pools for viral DNA replicationCell RepMay 26, 2026Lucas A. Simpson, Diana M. Dunn, Wyatt Fales, Zachary J. Moore, Jessica H. Ciesla, Nicole C. Waild, Matthew H. Raymonda, Isaac S. Harris, Joshua Munger#12259), and our lentiviral plasmid was incubated for 5 min. Following this incubation, FuGENE (Promega, E2691) was added to the mixture and incubated for 20 min. This solution was added to HEK 293T cells and incubated

(16) SIRT2 mediates integrated stress response by deacetylating and stabilizing 4E-BP1 to suppress translationEMBO ReportsMay 18, 2026Yanlin Zi, Jiaqi Zhao, Miao Wang, Dan Hou, Richard A Cerione, Hening LinDual-luciferase Reporter assay system,Promega,E1910 TM,Synthesized in the lab,Methods section Polyethyleneimine,Polysciences,24765 FuGene6,Promega,E2691 CellTiter-Glo® 2.0 Cell Viability Assay,Promega,G9241 Acetyl-Lysine Affinity Beads,Cytoskeleton Inc,AAC04 MG132,MedChemExpress,HY-13259 Bafilomycin A1

(17) Nickase NmCas9 unsilences paternal Ube3a in a mouse model of Angelman syndrome without causing AAV vector integrationScientific ReportsMay 14, 2026Hannah O. Bazick, Lucas M. James, Mark J. Zylkatransfected cells with 2000 ng of WT or mutant NmCas9-gRNA23 plasmids (described above) using FuGENE 6 (E2691, Promega). We added 1 mL of DMEM 24-hours later and then washed cells 24-hours later with PBS and dissociated

(18) Hierarchical small molecule inhibition of MYST acetyltransferasesNat CommunMay 13, 2026Xuemin Chen, Alexandra Castroverde, Minervo Perez, Ronald Holewinski, Kiall F. Suazo, Rashmi Karki, Thorkell Andresson, Benjamin A. Garcia, Jordan L. Meier(4.4*10^5 cells/dish in 4.4 ml DMEM media). After 24 h, plasmids were transfected using Fugene 6 (Promega, E2691) according to the manufacturer’s instructions: 202.8 μL of OPTIM, 17.2 μL of Fugene 6 and 2.3 μg of

(19) Engineering Murine Cross‐Reactivity Into an Affibody to Human Death Receptor 5Biotechnology and BioengineeringMay 6, 2026Tse‐Han Kuo, Nagamani Vunnam, Jonathan N. Sachs, Benjamin J. Hackelal. 2020) in 1149 µL Opti‐MEM (Gibco 51985091) with 69.6 µL FuGENE 6 Transfection Reagent (Promega E2691). All HEK293 cells were cultured in a completed growth medium with DMEM (Gibco 11965092) supplemented

(20) Hepatitis B virus covalently closed circular DNA formation in murine hepatic cells uncovers a late entry blockProceedings of the National Academy of SciencesApril 28, 2026Xupeng Hong, Georgia Brousseau, Hsuan-An Chen, Catherine A. Freije, Leon L. Seifert, Mengyin Zhang, Yingpu Yu, William M. Schneider, Ype P. de Jong, Alan McLachlan, et al.Transfection Reagent (Roche, catalog no. 6366236001) or FuGENE6 transfection reagent (Promega, catalog no. E2691; for Huh7) at 3:1 reagent:DNA ratio. Stable cell line generation. To generate stable cell lines using

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(1)

2/4/2020Best Transfection Reagent for Large-Sized DNA Sunita MeenaGood for large-sized DNA.
(1)

6/15/2016Fugene 6 Transfection Kit Benjamin GerovacThis kit is used to transfect mammalian cell lines. The kit is simple to use and results in high transfection efficiency.