FuGENE 6 Transfection Reagent from Promega

Product Specs
ItemFuGENE 6 Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2692
Quantity5 x 1ml
Transfection MethodNon-liposomal
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) An AIB1 isoform rewires glucocorticoid receptor signaling to promote TNBC progressioniScienceFebruary 20, 2026Amber J. Kiliti, Ghada M. Sharif, Megan E. McNamara, Raneen Rahhal, Susan Prewitt, Marcel O. Schmidt, Ci Wu, Junfeng Ma, Eric Glasgow, Anton Wellstein, et al.pcDNA3-AIB1Δ4 expression vector in the presence of FuGENE 6 Transfection Reagent (Promega Corporation, E2692). Cells were treated with either ethanol vehicle control or 10 nM of dexamethasone (Millipore Sigma,

(2) Identification and characterization of a novel inhibitor of influenza A virus that acts by blocking nucleoprotein oligomerizationAntimicrobial Agents and ChemotherapyFebruary 4, 2026Vincent H. J. Leonard, Dianna B. Vidales, Benjamin R. Taft, Matthew J. Hesse, Patrick S. Lee, Mulugeta Mamo, Dirksen E. Bussiere, Karen C. Wolff, Kelli L. Kuhen, Laura Wedel, et al.cells/well) or 384-well plates (1.8 × 104 cells/well) using Fugene 6 transfection reagent (Promega, cat #E2692) for 293T cells and TransIT-293 transfection reagent (Mirus, cat #MIR2700) for HEK293T/17 cells, according

(3) Small molecule inhibition rescues the skeletal dysplasia phenotype of Trpv4 mutant miceJCI InsightJanuary 23, 2026Lisette Nevarez, Taylor K. Ismaili, Jennifer Zieba, Jorge Martin, Davis Wachtell, Derick Diaz, Jocelyn A. Ramirez, Valeria Aceves, Joshua Ito, Ryan S. Gray, et al.cDNA or 5.6 μg hTRPV4 WT cDNA, and transfection reagent. The transfection reagent, FUGENE 6 (Promega, E2692), was added so the final ratio in the macrotube was 6 μL FUGENE 6 per 1 μg cDNA. During the cDNA:FUGENE

(4) Development of a split-toxin CRISPR screening platform to systematically identify regulators of human myoblast fusionNature CommunicationsJanuary 15, 2026Haifeng Zhang, Renjie Shang, Zheng Zhang, Min Zhou, Anne Bigot, Yanqing Cai, Yanran Zhao, Yushu Wang, Aaryahi Deshmukh, Elena Kudryashova, et al.transfecting human embryonic kidney 293 cells with the retroviral plasmid using FuGENE 6 (Promega, E2692). Viral supernatant was harvested two days post-transfection, filtered, and used to infect target cells

(5) Neutralizing antibody responses to the primary series of a COVID-19 mRNA vaccine in the cancer cohort under active treatmentHuman Vaccines & ImmunotherapeuticsDecember 31, 2025Huijing Xue, Troy J. Kemp, Chenglei Li, Nancy V. Roche, Ziad Bakouny, Chris Labaki, Guo-Shou Mary Lee, Rachel Trowbridge, Sarah Strauss, Melissa E. Hughes, et al.was produced in HEK293T/17 cells (ATCC, Cat# CRL-11268) by transfection using Fugene 6 (Promega, Cat# E2692) and a combination of spike plasmid, pCMVDR8.2 and pHR’ CMV Luc in a 1:17:17 ratio in Opti-MEM (Thermo Fisher Scientific

(6) SM08502-Mediated β-Catenin Repression Synergizes with Olaparib to Inhibit Tumor ProgressionCancer Res CommunDecember 1, 2025Bradley R. Corr, Elizabeth R. Woodruff, Tomomi M. Yamamoto, Kimberly R. Jordan, Thomas Danhorn, Carine Bossard, Lily L. Nguyen, Edward B. Chuong, Lars Wick, Alexandra Young, et al.Luciferase Assay System (cat. E1501; Promega) and TOP-FLASH, FOP-FLASH plasmids (11, 20). Using FuGENE6 reagent (cat. #E2692; Promega), populations were transfected with TOP-FLASH or FOP-FLASH plasmid. M50 Super 8x TOPFlash and

(7) Optimal CXCR5 expression during Tfh maturation involves the Bhlhe40-Pou2af1 axisCell ReportsNovember 25, 2025Xiaoliang Zhu, Xi Chen, Yaqiang Cao, Chengyu Liu, Zoey J. Kline, Gangqing Hu, Sundar Ganesan, Tibor Z. Veres, Difeng Fang, Shuai Liu, et al.HEPES 1M,Gibco,Cat#15630-080 β-mercaptoethanol,Thermofisher,Cat#31350010 Penicillin Streptomycin,Gibco,Cat#15140-122 FuGENE® 6 Transfection Reagent,Promega,Cat#E2692 Polybrene Infection/Transfection Reagent,sigma,Cat#TR-1003-G Cytofix™ Fixation Buffer (554655; BD Bioscience

(8) Structure of European Robin Cryptochrome 1 reveals a Role in Circadian Rhythms, Not MagnetoreceptioniScienceNovember 11, 2025Anushka C. Wickramaratne, Emil Sjulstok Rasmussen, Yogarany Chelliah, Fabian Schuhmann, Ilia A. Solov’yov, Henrik Mouritsen, Carla B. Green, Brian D. Zoltowski, Joseph S. Takahashiproteins" ,, "D-Luciferin Firefly, sodium salt monohydrate",Biosynth,Cat#L8240 FuGENE 6,Promega,Cat#E2692 Dexamethasone,Sigma-Aldrich,Cat#D4902 Powdered DMEM without phenol red,Corning,Cat# 90-013-PB Protease inhibitors

(9) CRISPR Screen Identifies BAP1 as a Deubiquitinase Regulating SPIN4 StabilityBiochemistryOctober 21, 2025Alondra Sanchez, Chen Zhou, Rima Tulaiha, Francisco Ramirez, Lu Wang, Xiaoyu ZhangSanta Cruz Biotechnology. Cas9 endonuclease was purchased from Integrated DNA Technologies (cat. #1081061). FuGene 6 (cat. #E2692) transfection reagent and sequencing grade trypsin/Lys-C mix (cat. #V5071) were purchased from Promega.

(10) Proliferative proteome induced by HPV 16E6 and NFX1-123 partnership in longitudinal keratinocyte culturesJournal of VirologySeptember 23, 2025C. L. Billingsley, E. H. Doud, W. Smith-Kinnaman, A. L. Mosley, S. Chintala, R. A. Katzenellenbogendescribed (44). After incubating the viral plasmids and plasmids of interest with FuGENE6 (Promega, E2692) in 293T cells, the viral supernatants were collected over 48 h. For transduction, viral stocks were

(11) Hepatitis B surface antigen is upregulated by HIV Tat in an HIV-hepatitis B virus co-infection model systemMicrobiology spectrumSeptember 2, 2025Zhao W, Singh K, Sozzi V, Cevaal P, Rossello FJ, Martelotto L, Zerbato JM, Rhodes A, Powell DR, Schröder J, et al.(NIH AIDS reagent program) and 4 µg of the envelope plasmid expressing VSV.G using 60 µL FuGENE 6 (E2692; Promega, Madison, WI) according to the manufacturer’s instructions. After overnight incubation, the

(12) Optogenetic and chemical genetic tools for rapid repositioning of vimentin intermediate filamentsJ Cell BiolSeptember 1, 2025Milena Pasolli, Joyce C.M. Meiring, James P. Conboy, Gijsje H. Koenderink, Anna Akhmanovanumber SiR-tubulin,Spirochrome,SC014 Janelia Fluor 646 HaloTag ligand,Promega,GA1120 FuGENE 6,Promega,E2692 Pen/strep,Gibco,15140-122 Gibson assembly master mix,Thermo Fisher Scientific,A46629 ProLong glass

(13) Vaccination with an mRNA-encoded membrane-bound HIV envelope trimer induces neutralizing antibodies in animal modelsScience Translational MedicineJuly 30, 2025Parham Ramezani-Rad, Christopher A. Cottrell, Ester Marina-Zárate, Alessia Liguori, Elise Landais, Jonathan L. Torres, Amber Myers, Jeong Hyun Lee, Sabyasachi Baboo, Claudia Flynn, et al.of interest and the Env-deficient HIV-1 backbone plasmid pSG3ΔEnv, using either Fugene 6 (Promega, E2692) or PEI MAX (Polysciences, 24765–1). Viral supernatants were collected 72 hours post-transfection and

(14) The effect of amycretin, a unimolecular glucagon-like peptide-1 and amylin receptor agonist, on body weight and metabolic dysfunction in mice and ratsEBioMedicineJuly 23, 2025Rune Ehrenreich Kuhre, Borja Ballarín-González, Christian Lehn Brand, Tine Glendorf, Kim Grimstrup Madsen, Karina Rahr Hjøllund, Wouter Frederik Johan Hogendorf, David Højland Ipsen, Sofia Lundh, Thomas Kruse, et al.combination with a plasmid encoding mouse RAMP3 by use of FuGENE®6 transfection reagent (Promega, Cat no. E2692). After addition of plasmids and transfection reagent, cells were incubated for 24 h at 37 °C in 5%

(15) CRAMP1-dependent histone H1 biogenesis is essential for topoisomerase II inhibitor toleranceMolecular cellJuly 3, 2025Ingham A, de Vega IA, Morlot L, Gittens W, Hendriks IA, Kakulidis ES, Freire R, Davey NE, Duxin JP, Lund Nielsen M, et al.peptides, and recombinant proteins" ,, Lipofectamine 3000,Invitrogen,Cat# L3000015 Fugene 6,Promega,Cat# E2692 Lipofectamine RNAiMax,Invitrogen,Cat# 13778500 Etoposide,Sigma-Aldrich,Cat# E1383 ICRF-193,Sigma-Aldrich,Cat#

(16) Biased agonism of GLP-1R and GIPR enhances glucose lowering and weight loss, with dual GLP-1R/GIPR biased agonism yielding greater efficacyCell Rep MedJune 17, 2025Ruben Rodriguez, Anne Hergarden, Shyam Krishnan, Marikris Morales, Davina Lam, Ted Tracy, Teresa Tang, Avalon Patton, Craig Lee, Asmita Pant, et al.Substrate,Promega,N2012 Nano-Glo® HiBiT Extracellular Detection System,Promega,N2421 Fugene 6,Promega,E2692 U-PLEX Mouse Insulin Assay,Meso Scale Discovery,K1526HK-2 ,, Experimental models: Cell lines,Experimental models

(17) Parkin-dependent ubiquitination of TAX1BP1 directs efficient autophagic removal of defective mitochondriabioRxivMay 22, 2025Anna Lechado-Terradas, Bianca Lemke, Katharina I. Zittlau, Boris Macek, Philipp J. Kahlecell death. Transient transfection of the indicated constructs was performed with FuGENE6® (Promega, E2692), following manufacturer’s instructions. HeLa cells were transfected with 5 nM of siRNA for three consecutive

(18) Synchronized temporal-spatial analysis via microscopy and phosphoproteomics (STAMP) of quiescenceSci AdvApril 25, 2025Mohammad Ovais Azizzanjani, Rachel E. Turn, Anushweta Asthana, Karen Y. Linde-Garelli, Lucy Artemis Xu, Leilani E. Labrie, Mohammadamin Mobedi, Peter K. Jacksondvpr, and 500 ng of lentiviral transfer plasmids (previously described) along with 3 μl of Fugene 6 (E2692, Promega) transfection reagent. The medium was replaced 24 hours after transfection to prevent toxicity

(19) Parp3 assists muscle function and skeletal muscle differentiation by selectively adjusting H3K27me3 enrichmentiScienceApril 18, 2025Zuleyha Yildirim, Aurélia Noll, Kathline Martin-Hernandez, Jean-Christophe Amé, Najat Hanini, Nadia Messaddeq, Isabelle Robert, Bernardo Reina San Martin, Gunn Hildrestrand, Magnar Bjoras, et al.or Flag-Parp3HE, described in (19)) and 0.9 μg/μL of pCL-Ecotropic plasmid using Fugene 6 (Promega #E2692). Two days later, retroviral supernatants were added to C2C12 Parp3KO1 cells in the presence of Polybrene

(20) Tripartite motif-containing 27 negatively regulates NF-κB activation in bone remodelingMol MedApril 18, 2025Kabsun Kim, Jung Ha Kim, Inyoung Kim, Semun Seong, Hyun Kook, Jeong-Tae Koh, Nacksung Kimplasmids, and the parental pMX vector were transfected into the packaging cell line Plat-E using Fugene 6 (#E2692, Promega) according to the manufacturer’s protocol. Viral supernatant was collected from cultured media

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