FuGENE 6 Transfection Reagent from Promega

Product Specs
ItemFuGENE 6 Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2692
Quantity5 x 1ml
Transfection MethodNon-liposomal
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) NFX1-123 is required for keratinocyte differentiation and HPV 16 DNA maintenance in early and persistent infection modelsJournal of VirologyJuly 21, 2026Maura A. Dankoski, DeShawn Thompson, Kevin M. Quist, Rachel A. Katzenellenbogen(11). Briefly, the constructs were transfected into 293T cells using FuGENE6 (Promega, Madison, WI, US, E2692). After 24 h of transfection, media containing produced virus was serially collected four times (twice

(2) Kozak sequence libraries for systematically characterizing transgenes across expression levelsNucleic Acids ResJuly 20, 2026Nidhi Shukla, Nisha D Kamath, John C Snell, Anna M Bruchez, Kenneth A Matreyekof 1.2 μg plasmid of Bxb1 attB-encoding plasmid and 5 μl of Fugene 6 transfection reagent (Promega, E2692), in 100 μl total of Opti-MEM (Gibco, 31985070). Three days after transfection, non-recombined landing

(3) Dissecting the functional divergence of DCAF11 isoforms in protein degradationRSC Chem BiolJuly 10, 2026Xiaokang Jin, Xiaoyu Zhanganti-DCAF11 antibody (cat. #A15519, dilution 1 : 1000) was purchased from Abclonal. FuGene 6 (cat. #E2692) transfection reagent, Cell Titer Glo (cat. #G7573) and sequencing grade trypsin (cat. # V5111) and

(4) Induction of broadly neutralizing HIV antibodies by a two-step mechanism informs vaccine designScienceJune 18, 2026Ashwin N. Skelly, Harry B. Gristick, Hui Li, Edem Gavor, Andrew J. Connell, Edward F. Kreider, Lorie Marchitto, Michael P. Hogarty, Maddy L. Newby, Joel D. Allen, et al.generation) was diluted in 500 μL of DMEM, combined with 18 μL of FuGENE 6 transfection reagent (Promega E2692), and added dropwise to the cells. Cells were cultured for 48 hours at 37 °C with 5% CO2. Virus-containing

(5) Mechanism of RACK1-dependent ZAKα activation at stalled and collided ribosomesMolecular CellJune 18, 2026Anna Constance Vind, José Francisco Martínez, Zhenzhen Wu, Andrii Bugai, Kelly Mordente, Giancarlo Abis, Sébastien Chamois, Sofia Ramalho, Catarina Pechincha, Laura Ryder, et al.constructs were verified by sequencing, and all plasmid transfections were done using FUGENE6 (Promega, #E2692) according to the manufacturer’s protocol. HAP1 ΔGCN1 and ΔGCN2 cell lines were generated using CRISPR/Cas9

(6) Covalent Reprogramming of Kinase Binders to Modulate Protein AbundanceAdvanced ScienceJune 1, 2026Chen Mozes, Xiaokang Jin, Miguel A. Campos, Chen Zhou, Xiaoyu Zhang(TMT) isobaric label reagent (cat# 90110) were purchased from Thermo Fisher Scientific. FuGene6 (cat# E2692) transfection reagent and sequencing‐grade modified trypsin (cat# V5111) were purchased from Promega.

(7) TMPRSS2-induced Golgi disruption restricts the incorporation of virus envelope glycoproteins into virionsEMBO ReportsMay 19, 2026Sayuri Seki, Shigeyoshi Harada, Akiko Sugimoto-Ishige, Midori Unno, Machie Sakuma, Shinsuke Ito, Hiroyuki Yamamoto, Aki Tanabe, Saori Matsuoka, Chieko Makino-Okamura, et al.gift from Michael Davidson, Addgene#56590) (Olenych et al, 2007) expression plasmids using FuGene 6 (E2692, Promega) according to the manufacturer’s recommendations. The cells were fixed in 2% formaldehyde (Thermo

(8) Hematopoietic stem cells undergo bidirectional fate transitions in vivobioRxivApril 11, 2026Tsuyoshi Fukushima, Trine Ahn Kristiansen, Lai Ping Wong, Samuel Keyes, Yosuke Tanaka, Michael Mazzola, Ting Zhao, Lingli He, Masaki Yagi, Konrad Hochedlinger, et al.(Gibco, 31985–062) (900 μL), VSVG (3.5 μg), PAX2 (7 μg), vector (12 μg), and Fugene6 (67.5 μL) (Promega, E2692) were mixed and incubated at room temperature for 15 minutes, then added to the HEK293T cells. After

(9) An AIB1 isoform rewires glucocorticoid receptor signaling to promote TNBC progressioniScienceFebruary 20, 2026Amber J. Kiliti, Ghada M. Sharif, Megan E. McNamara, Raneen Rahhal, Susan Prewitt, Marcel O. Schmidt, Ci Wu, Junfeng Ma, Eric Glasgow, Anton Wellstein, et al.pcDNA3-AIB1Δ4 expression vector in the presence of FuGENE 6 Transfection Reagent (Promega Corporation, E2692). Cells were treated with either ethanol vehicle control or 10 nM of dexamethasone (Millipore Sigma,

(10) Identification and characterization of a novel inhibitor of influenza A virus that acts by blocking nucleoprotein oligomerizationAntimicrobial agents and chemotherapyFebruary 4, 2026Leonard VHJ, Vidales DB, Taft BR, Hesse MJ, Lee PS, Mamo M, Bussiere DE, Wolff KC, Kuhen KL, Wedel L, et al.cells/well) or 384-well plates (1.8 × 104 cells/well) using Fugene 6 transfection reagent (Promega, cat #E2692) for 293T cells and TransIT-293 transfection reagent (Mirus, cat #MIR2700) for HEK293T/17 cells, according

(11) Structural basis for pseudokinase-mediated regulation of GCN2 in the integrated stress responseProceedings of the National Academy of SciencesFebruary 3, 2026Yixin Liu, Jagannath Misra, Debarshi Ryan Bhowmik, Brady O’Boyle, Kirk A. Staschke, Natarajan Kannan, Ronald C. Wek, Natalia Juracontrol plasmid using FuGENE 6 transfection reagent in a 3:1 ratio of FuGENE 6 to DNA (Promega Cat #E2692). Cells were cultured in complete DMEM at 37 °C for another 24 h, then treated with 25 nM HF or DMSO

(12) Small molecule inhibition rescues the skeletal dysplasia phenotype of Trpv4 mutant miceJCI InsightJanuary 23, 2026Lisette Nevarez, Taylor K. Ismaili, Jennifer Zieba, Jorge Martin, Davis Wachtell, Derick Diaz, Jocelyn A. Ramirez, Valeria Aceves, Joshua Ito, Ryan S. Gray, et al.cDNA or 5.6 μg hTRPV4 WT cDNA, and transfection reagent. The transfection reagent, FUGENE 6 (Promega, E2692), was added so the final ratio in the macrotube was 6 μL FUGENE 6 per 1 μg cDNA. During the cDNA:FUGENE

(13) Rational Design of Multiclade Coronavirus Spike Immunodominant Domain Nanoparticles to Elicit Broad Antibody ResponsesResearch SquareJanuary 17, 2026Christian K.O. Corbett-Helaire, Christian Dzuvor, Lindsay R. McManus, Lindsay McManus, Abigail E. Dzordzorme, Abigail Dzordzorme, Taewoo Joseph, Valerie Joseph, Ingelise J. Gordon, Ingelise Gordon, et al.spike, S gene plasmids seeded in 150 mm tissue culture dishes using using fugene 6 transfection (# E2692, Promega, Madison, WI, USA) in OPTI-Minimum Essential Media (# 31985-070, ThermoFisher, Waltham, MA,

(14) Unconventional linkers facilitate potent stabilized coronavirus stem antibody responses following nanoparticle vaccinationbioRxivJanuary 16, 2026Christian K.O. Dzuvor, Sydney P. Moak, Lindsay R. McManus, Abigail E. Dzordzorme, Lamount R. Evanson, Taewoo Kim, Abigail Thomas, Olayimika Adeyemi, Valerie Foley, Aditi Limaye, et al.spike (S gene) plasmids seeded in 150 mm tissue culture dishes using FuGENE 6 transfection reagent (#E2692, Promega) in Opti-MEM Reduced Serum Medium (Gibco #31985–070). For SARS-CoV-2 and WIV1-CoV pseudoviruses,

(15) Development of a split-toxin CRISPR screening platform to systematically identify regulators of human myoblast fusionNature CommunicationsJanuary 15, 2026Haifeng Zhang, Renjie Shang, Zheng Zhang, Min Zhou, Anne Bigot, Yanqing Cai, Yanran Zhao, Yushu Wang, Aaryahi Deshmukh, Elena Kudryashova, et al.transfecting human embryonic kidney 293 cells with the retroviral plasmid using FuGENE 6 (Promega, E2692). Viral supernatant was harvested two days post-transfection, filtered, and used to infect target cells

(16) Neutralizing antibody responses to the primary series of a COVID-19 mRNA vaccine in the cancer cohort under active treatmentHuman Vaccines & ImmunotherapeuticsDecember 31, 2025Huijing Xue, Troy J. Kemp, Chenglei Li, Nancy V. Roche, Ziad Bakouny, Chris Labaki, Guo-Shou Mary Lee, Rachel Trowbridge, Sarah Strauss, Melissa E. Hughes, et al.was produced in HEK293T/17 cells (ATCC, Cat# CRL-11268) by transfection using Fugene 6 (Promega, Cat# E2692) and a combination of spike plasmid, pCMVDR8.2 and pHR’ CMV Luc in a 1:17:17 ratio in Opti-MEM (Thermo Fisher Scientific

(17) SM08502-Mediated β-Catenin Repression Synergizes with Olaparib to Inhibit Tumor ProgressionCancer Res CommunDecember 1, 2025Bradley R. Corr, Elizabeth R. Woodruff, Tomomi M. Yamamoto, Kimberly R. Jordan, Thomas Danhorn, Carine Bossard, Lily L. Nguyen, Edward B. Chuong, Lars Wick, Alexandra Young, et al.Luciferase Assay System (cat. E1501; Promega) and TOP-FLASH, FOP-FLASH plasmids (11, 20). Using FuGENE6 reagent (cat. #E2692; Promega), populations were transfected with TOP-FLASH or FOP-FLASH plasmid. M50 Super 8x TOPFlash and

(18) SM08502-Mediated β-Catenin Repression Synergizes with Olaparib to Inhibit Tumor ProgressionCancer Res CommunDecember 1, 2025Bradley R. Corr, Elizabeth R. Woodruff, Tomomi M. Yamamoto, Kimberly R. Jordan, Thomas Danhorn, Carine Bossard, Lily L. Nguyen, Edward B. Chuong, Lars Wick, Alexandra Young, et al.Luciferase Assay System (cat. E1501; Promega) and TOP-FLASH, FOP-FLASH plasmids (11, 20). Using FuGENE6 reagent (cat. #E2692; Promega), populations were transfected with TOP-FLASH or FOP-FLASH plasmid. M50 Super 8x TOPFlash and

(19) Optimal CXCR5 expression during Tfh maturation involves the Bhlhe40-Pou2af1 axisCell ReportsNovember 25, 2025Xiaoliang Zhu, Xi Chen, Yaqiang Cao, Chengyu Liu, Zoey J. Kline, Gangqing Hu, Sundar Ganesan, Tibor Z. Veres, Difeng Fang, Shuai Liu, et al.HEPES 1M,Gibco,Cat#15630-080 β-mercaptoethanol,Thermofisher,Cat#31350010 Penicillin Streptomycin,Gibco,Cat#15140-122 FuGENE® 6 Transfection Reagent,Promega,Cat#E2692 Polybrene Infection/Transfection Reagent,sigma,Cat#TR-1003-G Cytofix™ Fixation Buffer (554655; BD Bioscience

(20) Structure of European Robin Cryptochrome 1 reveals a Role in Circadian Rhythms, Not MagnetoreceptioniScienceNovember 11, 2025Anushka C. Wickramaratne, Emil Sjulstok Rasmussen, Yogarany Chelliah, Fabian Schuhmann, Ilia A. Solov’yov, Henrik Mouritsen, Carla B. Green, Brian D. Zoltowski, Joseph S. Takahashiproteins" ,, "D-Luciferin Firefly, sodium salt monohydrate",Biosynth,Cat#L8240 FuGENE 6,Promega,Cat#E2692 Dexamethasone,Sigma-Aldrich,Cat#D4902 Powdered DMEM without phenol red,Corning,Cat# 90-013-PB Protease inhibitors

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