Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Research Areas
ELISA
Western Blot
3D Cell Culture
Digital PCR
Quantitative PCR
Cell and Gene Therapy
In Vivo Models
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
White Papers
Infographics
Events
Promotions
Multimedia
Biocompare Webinars
Technologies Transforming Precision Medicine
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Register
Sign In
Biocompare
Products
Protein Biochemistry
Chemicals and Reagents
FOXP3 Fix/Perm Buffer Set
FOXP3 Fix/Perm Buffer Set from BioLegend
Be the first to write a review!
Citations:
(142)
Supplier Page
Supplier Page from
BioLegend for
FOXP3 Fix/Perm Buffer Set
Description
FOXP3 Fix/Perm Buffer Set
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
FOXP3 Fix/Perm Buffer Set
Company
BioLegend
Price
$141.00
Supplier Page
View Company Product Page
Catalog Number
421403
Quantity
100 tests
Applications
ICFC - Quality tested
Target
FOXP3
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (142)
(1)
Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cells
Cell
April 20, 2026
Ujjwal Rathore, Eli Dugan, Hunter Thornton, Vigneshwari Easwar Kumar, Rama Dajani, Ryan C. Burdick, Janet M. Young, Zachary Steinhart, Reanna Lao, Krista A. Delviks-Frankenberry,
et al.
EasySep Human CD4+ T Cell Isolation Kit
,
STEMCELL Technologies
,Cat# 100-0696 "
FOXP3 Fix/Perm Buffer Set
, 100 tests",
BioLegend
,Cat#
421403
P3 Primary Cell 96-well Nucleofector Kit
(960 RCT),
Lonza
,Cat# V4SP-3960
Phusion High-Fidelity PCR Master Mix with HF Buffer
(2)
Distinct dendritic cell cytoskeletal programs dictate synapse architecture and CD8+ T cell fate
Front Immunol
April 1, 2026
Camille D. Clamagirand, Christoph Ratswohl, Marc W. Schmid, Theresia Eich, Nadine Anslinger, Daniel F. Legler, Jérémie Rossy
(
BD
,
554714
) and intranuclear transcription factor
staining
via
FOXP3 Fix/Perm Buffer Set
(
Biolegend
,
421403
) following manufacturer’s instructions. Ultimately, 5,000 events of viable
T cells
were measured on
(3)
Direct contact with natural killer cells reprograms monocyte-derived dendritic cells into a tolerogenic phenotype
Immunohorizons
March 26, 2026
Ammar J Alsheikh, Liang Chen, Daniel Korenfeld, Jason Tam, Suresh Patil, Hsi-Ju Wei, Jianing Li, Suju Zhong, Feng Dong, Terry Melim,
et al.
Cat#
109-136-088
). Intracellular
staining
was performed using the
FOXP3 Fix/Perm Buffer Set
(
BioLegend
, Cat#
421403
) after the surface
staining
. Snarf-1 dye, which also functions as a sensitive pH indicator, was used
(4)
A combination of proviral and antiviral roles of CD11c- and T-bet-expressing B cells defines parameters of chronic murine gammaherpesvirus infection
mBio
March 11, 2026
Erika R. Johansen, Xander G. Bradeen, Emily V. Xie, Bonnie N. Dittel, Elizabeth A. Leadbetter, Vera L. Tarakanova
For intracellular
staining
, cells were fixed and permeabilized using
FOXP3 Fix/Perm Buffer Set
(cat:
421403
;
BioLegend
[San Diego, CA]) according to the manufacturer’s instructions, and cells were stained for
(5)
Loss of MicroRNA-29b promotes DNMT3b-mediated STING downregulation to attenuate radiotherapy-induced antitumor immunity in KRAS-mutated colorectal cancer
npj Precision Oncology
January 28, 2026
Hsin-Yu Chang, Jhen-Yu Chen, Tao-Wei Ke, Yi-Wen Jiang, Wei-Ze Hong, Yuan-Yao Tsai, Shu-Fen Chiang, Yu-Sen Lin, Chi-Hsien Huang, Te-Hong Chen,
et al.
intracellular marker
Foxp3
was fixed and permeabilized with a
FOXP3
Fix/Perm buffer before
staining
(Cat#
421403
,
Biolegend
). The intracellular marker INFγ was activated by the protein transport
inhibitor
brefeldin
(6)
Axes of biological variation in diffuse large B cell lymphoma
Cancer Cell
January 15, 2026
Boya Wang, George Wright, Julius C. Enssle, Xin Yu, James D. Phelan, Yandan Yang, Arthur Shaffer, Arvin E. Ruiz, Da Wei Huang, Zana Coulibaly,
et al.
GEM Single Cell 5’ Kit v2,10X Genomics,Cat.# PN-1000265
FOXP3 Fix/Perm Buffer Set
,
Biolegend
,Cat.#
421403
Deposited data,, Bulk RNA and
WES
of DLBCL,This publication,EGAS50000001227 Single cell RNA and ATAC
(7)
FOXP3 expression depends on cell-type-specific cis-regulatory elements and transcription factor circuitry
Immunity
January 13, 2026
Jennifer M. Umhoefer, Maya M. Arce, Sivakanthan Kasinathan, Sean Whalen, Rama Dajani, Sanjana Subramanya, Laine Goudy, Julia A. Belk, Royce Zhou, Minh T. N. Pham,
et al.
removed. Intracellular
staining
was conducted using the
FOXP3 Fix/Perm Buffer Set
(
Biolegend
, catalog no.
421403
). Kit components were diluted in DPBS according to manufacturer’s protocol. Cells were resuspended in
(8)
Hyperglycemia drives lmmune evasion in colorectal cancer through VISTA stabilization
Cell Communication and Signaling
January 7, 2026
Xin Liu, Qian Liu, Dong Guo, Zezhou Zhao, Jing Wang, Yaoxian Xiang, Kangjie Wang, Lijun Yang, Ke Zhu, Li Wang,
et al.
Subsequently, the cells were fixed and permeabilized using Fixation & Permeabilization Buffer (
Biolegend
; Cat#
421403
), and stained for 30 min with the following antibodies:
APC anti-human CD8
(
Biolegend
; Cat#
344722
);
(9)
Type I interferon restricts mRNA vaccine efficacy through suppression of antigen uptake in cDCs
npj Vaccines
January 5, 2026
Tyson A. Lobb, Alexandria Dickson, Wenzheng Guo, Smrithi Beeram, Javier A. Carrero, Yago Dalben, Richard J. DiPaolo, Elise Alspach, Longping V. Tse, Stephen T. Ferris
antibodies. Cells were then washed before being fixed with the
FoxP3 Fix/Perm Buffer set
(
Biolegend
421403
) for 20 min at room temperature. Then cells were stained with intracellular antibody IFNγ in perm buffer
(10)
A Brain Endothelial Cell Caveolin-1/CXCL10 Axis Promotes T Cell Transcellular Migration Across the Blood-Brain Barrier
ASN Neuro
December 31, 2025
Troy N. Trevino, Ali A. Almousawi, Remy Martins-Goncalves, Andrea Ochoa-Raya, KaReisha F. Robinson, Genesis L. Abad, Leon M. Tai, Suellen D. Oliveira, Richard D. Minshall, Sarah E. Lutz
(
Biolegend
150627
,
1:100
),
CD11b
(
Biolegend
101229
,
1:100
). Fixation and permeabilization (
Biolegend
421403
) was followed with an antibody against
Cav-1
(
Cell Signaling
31411S
). Cells were analyzed with a Beckman
(11)
B cell-intrinsic IRF-1 and conserved gammaherpesvirus protein kinase cooperate to promote murine gammaherpesvirus-driven germinal center response and splenic latent reservoir
Journal of Virology
December 23, 2025
Cade R. Rahlf, Christopher N. Jondle, Erika R. Johansen, Vera L. Tarakanova
intracellular detection of γH2AX, cell permeabilization was performed using
FOXP3 Fix/Perm Buffer Set
(cat:
421403
;
BioLegend
(San Diego, CA)) followed by 1 hour
incubation
with optimized antibody concentration at room
(12)
Persistent glycolysis defines pathological foreign body-associated inflammation to polymeric implants
Sci Adv
December 19, 2025
Christian Rempe, Neal Callaghan, Lauren Fong-Hollohan, Sarah Nersesian, Zachary Froom, Kyle Medd, Ibrahim Ahmed, Tobias Karakach, Jeanette E. Boudreau, Michael Bezuhly,
et al.
serum (FBS) (heat inactivated,
Corning
,
35-011-CV
), FoxP3 Fixation/Permeabilization Buffer (
BioLegend
,
421403
), FoxP3 Permeabilization Solution (BioLegend,
421403
),
Harringtonine
(
Abcam
,
AB141941
), Histoplast PE
(13)
Physical activity decreases cancer burden by alleviating immunosenescence-related inflammation and improving overall immunity
Cell Rep Med
December 16, 2025
Yi Jin, Zhiyu Yang, Zishuai Li, Zhan Ding, Xueyi Zhang, Huixian Zeng, Letian Fang, Yiwei Shi, Puyi Xing, Wenbin Liu,
et al.
staining
, the cells were then fixed and permeabilized using the
FOXP3 Fix/Perm Buffer Set
(
BioLegend
, Cat#
421403
), according to the manufacturer’s protocol. Following permeabilization, cells were incubated with antibodies
(14)
MyD88 Deficiency Protects Mice From Experimental Autoimmune Encephalomyelitis by Influencing Both Dendritic Cells and T Cells
Immunology
December 9, 2025
Wen Si, Gaochen Zhu, Qianling Jiang, Xin Ma, Guan Yang
antibodies against
mouse
FoxP3
(
eBioscience
,
11–5773‐82
) and the
FoxP3 Fix/Perm Buffer Set
(
Biolegend
,
421403
). To detect the expression of IL‐17A, IFN‐γ, and GM‐CSF in CD4+ T cells, splenocytes were first treated
(15)
Genistein enhances anti-PD-1 efficacy in melanoma by suppressing regulatory T cell differentiation and activity
Sci Rep
October 22, 2025
Fei Mo, Min Hong, Xiao Chen, Qiye Wang, Chao Dong
staining
, cells were fixed and permeabilized using the Foxp3 Transcription Factor Staining Buffer Set (
421403
,
BioLegend
, San Diego, CA, USA) and subsequently stained with APC-αFoxp3 (
1:100
). After three washes
(16)
Superinfection promotes replication and diversification of defective HIV-1 proviruses in people with non-suppressible viraemia
Nature Microbiology
October 3, 2025
Vivek Hariharan, Jennifer A. White, Filippo Dragoni, Emily J. Fray, Nicholas Pathoulas, Milica Moskovljevic, Hao Zhang, Anushka Singhal, Jun Lai, Subul A. Beg,
et al.
(
ThermoFisher
,
L34975
). Cells were then fixed and permeabilized with the FOXP3 Buffer Set (
Biolegend
,
421403
), followed by intracellular
staining
of HIV-1 p24 with
clone
28B7 APC (MediMabs, MM-0289-APC) and
clone
(17)
Isolation of a Monoclonal Human scFv Against Cytomegalovirus pp71 Antigen Using Yeast Display
Antibodies (Basel)
July 10, 2025
Kazuhisa Aoki, Rikio Yabe, Sayaka Ono, Mayumi Saeki, Yuri Tanno, Hidetaka Tanno
transfection
, the cells were fixed and permeabilized with a
FOXP3 Fix/Perm Buffer Set
(
BioLegend
, #
421403
). The cells were incubated with the ID1 or ID2 scFv-Fc (1.3 μg/mL) and then stained with
FITC anti-His tag
(18)
Interferon-γ orchestrates leptomeningeal anti-tumour response
Nature
July 1, 2025
Remsik J, Tong X, Kunes RZ, Li MJ, Estrera R, Snyder J, Thomson C, Osman AM, Chabot K, Sener UT,
et al
.
processed using True-Nuclear Buffer Set (
BioLegend
,
424401
) or
FOXP3 Fix/Perm buffer set
(
BioLegend
,
421403
) and analysed. MHC class I levels of vehicle- or recombinant
mouse
IFNγ-exposed (BioLegend,
714006
)
(19)
α-GalCer-LNP ehanced mRNA delivery and activates natural killer T cells for superior tumor immunotherapy
Materials Today Bio
June 1, 2025
Kexin Zhang, Zhen Xu, Zexiu Xiao, Fei Cao, Jing Wang, Yuandong Xu, Winson M.J. Ma, Sihui Long, Gao-Feng Zha
(e.g., PLZF) were detected using Cyto-Fast Fix/Perm (
BioLegend
750000135) and FoxP3 Fix/Perm (
BioLegend
421403
) kits, respectively, after surface
staining
, followed by intracellular/intranuclear antibody
incubation
(20)
LncRNA TCL6 regulates miR-876–5p/MYL2 axis to suppress breast cancer progression
Translational Oncology
March 1, 2025
YaoBang Liu, Hong Li, DaHai Chai, Bin Lian, ZhengYang Bai, YaLi Gao, JinPing Li
cytokine
staining
. For nuclear
protein staining
, the cells were resuspended in 1x FOXP3 Fix/Perm buffer (
421403
,
Biolegend
, USA), mixed, and incubated at room temperature for 20 min. The cells were then resuspended
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Be the First to Write a Review!
FOXP3 Fix/Perm Buffer Set from BioLegend
Supplier Page
View Company Product Page
$141.00
Product Specs
Company Info
Citations
Reviews
Back To Top