FOXP3 Fix/Perm Buffer Set from BioLegend

Product Specs
ItemFOXP3 Fix/Perm Buffer Set
CompanyBioLegend
Price $141.00Supplier Page View Company Product Page
Catalog Number421403
Quantity100 tests
ApplicationsICFC - Quality tested
TargetFOXP3

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cellsCellApril 20, 2026Ujjwal Rathore, Eli Dugan, Hunter Thornton, Vigneshwari Easwar Kumar, Rama Dajani, Ryan C. Burdick, Janet M. Young, Zachary Steinhart, Reanna Lao, Krista A. Delviks-Frankenberry, et al.EasySep Human CD4+ T Cell Isolation Kit,STEMCELL Technologies,Cat# 100-0696 "FOXP3 Fix/Perm Buffer Set, 100 tests",BioLegend,Cat# 421403 P3 Primary Cell 96-well Nucleofector Kit (960 RCT),Lonza,Cat# V4SP-3960 Phusion High-Fidelity PCR Master Mix with HF Buffer

(2) Distinct dendritic cell cytoskeletal programs dictate synapse architecture and CD8+ T cell fateFront ImmunolApril 1, 2026Camille D. Clamagirand, Christoph Ratswohl, Marc W. Schmid, Theresia Eich, Nadine Anslinger, Daniel F. Legler, Jérémie Rossy(BD, 554714) and intranuclear transcription factor staining via FOXP3 Fix/Perm Buffer Set (Biolegend, 421403) following manufacturer’s instructions. Ultimately, 5,000 events of viable T cells were measured on

(3) Direct contact with natural killer cells reprograms monocyte-derived dendritic cells into a tolerogenic phenotypeImmunohorizonsMarch 26, 2026Ammar J Alsheikh, Liang Chen, Daniel Korenfeld, Jason Tam, Suresh Patil, Hsi-Ju Wei, Jianing Li, Suju Zhong, Feng Dong, Terry Melim, et al.Cat#109-136-088). Intracellular staining was performed using the FOXP3 Fix/Perm Buffer Set (BioLegend, Cat#421403) after the surface staining. Snarf-1 dye, which also functions as a sensitive pH indicator, was used

(4) A combination of proviral and antiviral roles of CD11c- and T-bet-expressing B cells defines parameters of chronic murine gammaherpesvirus infectionmBioMarch 11, 2026Erika R. Johansen, Xander G. Bradeen, Emily V. Xie, Bonnie N. Dittel, Elizabeth A. Leadbetter, Vera L. TarakanovaFor intracellular staining, cells were fixed and permeabilized using FOXP3 Fix/Perm Buffer Set (cat: 421403; BioLegend [San Diego, CA]) according to the manufacturer’s instructions, and cells were stained for

(5) Loss of MicroRNA-29b promotes DNMT3b-mediated STING downregulation to attenuate radiotherapy-induced antitumor immunity in KRAS-mutated colorectal cancernpj Precision OncologyJanuary 28, 2026Hsin-Yu Chang, Jhen-Yu Chen, Tao-Wei Ke, Yi-Wen Jiang, Wei-Ze Hong, Yuan-Yao Tsai, Shu-Fen Chiang, Yu-Sen Lin, Chi-Hsien Huang, Te-Hong Chen, et al.intracellular marker Foxp3 was fixed and permeabilized with a FOXP3 Fix/Perm buffer before staining (Cat# 421403, Biolegend). The intracellular marker INFγ was activated by the protein transport inhibitor brefeldin

(6) Axes of biological variation in diffuse large B cell lymphomaCancer CellJanuary 15, 2026Boya Wang, George Wright, Julius C. Enssle, Xin Yu, James D. Phelan, Yandan Yang, Arthur Shaffer, Arvin E. Ruiz, Da Wei Huang, Zana Coulibaly, et al.GEM Single Cell 5’ Kit v2,10X Genomics,Cat.# PN-1000265 FOXP3 Fix/Perm Buffer Set,Biolegend,Cat.# 421403 Deposited data,, Bulk RNA and WES of DLBCL,This publication,EGAS50000001227 Single cell RNA and ATAC

(7) FOXP3 expression depends on cell-type-specific cis-regulatory elements and transcription factor circuitryImmunityJanuary 13, 2026Jennifer M. Umhoefer, Maya M. Arce, Sivakanthan Kasinathan, Sean Whalen, Rama Dajani, Sanjana Subramanya, Laine Goudy, Julia A. Belk, Royce Zhou, Minh T. N. Pham, et al.removed. Intracellular staining was conducted using the FOXP3 Fix/Perm Buffer Set (Biolegend, catalog no. 421403). Kit components were diluted in DPBS according to manufacturer’s protocol. Cells were resuspended in

(8) Hyperglycemia drives lmmune evasion in colorectal cancer through VISTA stabilizationCell Communication and SignalingJanuary 7, 2026Xin Liu, Qian Liu, Dong Guo, Zezhou Zhao, Jing Wang, Yaoxian Xiang, Kangjie Wang, Lijun Yang, Ke Zhu, Li Wang, et al.Subsequently, the cells were fixed and permeabilized using Fixation & Permeabilization Buffer (Biolegend; Cat# 421403), and stained for 30 min with the following antibodies: APC anti-human CD8 (Biolegend; Cat# 344722);

(9) Type I interferon restricts mRNA vaccine efficacy through suppression of antigen uptake in cDCsnpj VaccinesJanuary 5, 2026Tyson A. Lobb, Alexandria Dickson, Wenzheng Guo, Smrithi Beeram, Javier A. Carrero, Yago Dalben, Richard J. DiPaolo, Elise Alspach, Longping V. Tse, Stephen T. Ferrisantibodies. Cells were then washed before being fixed with the FoxP3 Fix/Perm Buffer set (Biolegend 421403) for 20 min at room temperature. Then cells were stained with intracellular antibody IFNγ in perm buffer

(10) A Brain Endothelial Cell Caveolin-1/CXCL10 Axis Promotes T Cell Transcellular Migration Across the Blood-Brain BarrierASN NeuroDecember 31, 2025Troy N. Trevino, Ali A. Almousawi, Remy Martins-Goncalves, Andrea Ochoa-Raya, KaReisha F. Robinson, Genesis L. Abad, Leon M. Tai, Suellen D. Oliveira, Richard D. Minshall, Sarah E. Lutz(Biolegend 150627, 1:100), CD11b (Biolegend 101229, 1:100). Fixation and permeabilization (Biolegend 421403) was followed with an antibody against Cav-1 (Cell Signaling 31411S). Cells were analyzed with a Beckman

(11) B cell-intrinsic IRF-1 and conserved gammaherpesvirus protein kinase cooperate to promote murine gammaherpesvirus-driven germinal center response and splenic latent reservoirJournal of VirologyDecember 23, 2025Cade R. Rahlf, Christopher N. Jondle, Erika R. Johansen, Vera L. Tarakanovaintracellular detection of γH2AX, cell permeabilization was performed using FOXP3 Fix/Perm Buffer Set (cat: 421403; BioLegend (San Diego, CA)) followed by 1 hour incubation with optimized antibody concentration at room

(12) Persistent glycolysis defines pathological foreign body-associated inflammation to polymeric implantsSci AdvDecember 19, 2025Christian Rempe, Neal Callaghan, Lauren Fong-Hollohan, Sarah Nersesian, Zachary Froom, Kyle Medd, Ibrahim Ahmed, Tobias Karakach, Jeanette E. Boudreau, Michael Bezuhly, et al.serum (FBS) (heat inactivated, Corning, 35-011-CV), FoxP3 Fixation/Permeabilization Buffer (BioLegend, 421403), FoxP3 Permeabilization Solution (BioLegend, 421403), Harringtonine (Abcam, AB141941), Histoplast PE

(13) Physical activity decreases cancer burden by alleviating immunosenescence-related inflammation and improving overall immunityCell Rep MedDecember 16, 2025Yi Jin, Zhiyu Yang, Zishuai Li, Zhan Ding, Xueyi Zhang, Huixian Zeng, Letian Fang, Yiwei Shi, Puyi Xing, Wenbin Liu, et al.staining, the cells were then fixed and permeabilized using the FOXP3 Fix/Perm Buffer Set (BioLegend, Cat# 421403), according to the manufacturer’s protocol. Following permeabilization, cells were incubated with antibodies

(14) MyD88 Deficiency Protects Mice From Experimental Autoimmune Encephalomyelitis by Influencing Both Dendritic Cells and T CellsImmunologyDecember 9, 2025Wen Si, Gaochen Zhu, Qianling Jiang, Xin Ma, Guan Yangantibodies against mouse FoxP3 (eBioscience, 11–5773‐82) and the FoxP3 Fix/Perm Buffer Set (Biolegend, 421403). To detect the expression of IL‐17A, IFN‐γ, and GM‐CSF in CD4+ T cells, splenocytes were first treated

(15) Genistein enhances anti-PD-1 efficacy in melanoma by suppressing regulatory T cell differentiation and activitySci RepOctober 22, 2025Fei Mo, Min Hong, Xiao Chen, Qiye Wang, Chao Dongstaining, cells were fixed and permeabilized using the Foxp3 Transcription Factor Staining Buffer Set (421403, BioLegend, San Diego, CA, USA) and subsequently stained with APC-αFoxp3 (1:100). After three washes

(16) Superinfection promotes replication and diversification of defective HIV-1 proviruses in people with non-suppressible viraemiaNature MicrobiologyOctober 3, 2025Vivek Hariharan, Jennifer A. White, Filippo Dragoni, Emily J. Fray, Nicholas Pathoulas, Milica Moskovljevic, Hao Zhang, Anushka Singhal, Jun Lai, Subul A. Beg, et al.(ThermoFisher, L34975). Cells were then fixed and permeabilized with the FOXP3 Buffer Set (Biolegend, 421403), followed by intracellular staining of HIV-1 p24 with clone 28B7 APC (MediMabs, MM-0289-APC) and clone

(17) Isolation of a Monoclonal Human scFv Against Cytomegalovirus pp71 Antigen Using Yeast DisplayAntibodies (Basel)July 10, 2025Kazuhisa Aoki, Rikio Yabe, Sayaka Ono, Mayumi Saeki, Yuri Tanno, Hidetaka Tannotransfection, the cells were fixed and permeabilized with a FOXP3 Fix/Perm Buffer Set (BioLegend, #421403). The cells were incubated with the ID1 or ID2 scFv-Fc (1.3 μg/mL) and then stained with FITC anti-His tag

(18) Interferon-γ orchestrates leptomeningeal anti-tumour responseNatureJuly 1, 2025Remsik J, Tong X, Kunes RZ, Li MJ, Estrera R, Snyder J, Thomson C, Osman AM, Chabot K, Sener UT, et al.processed using True-Nuclear Buffer Set (BioLegend, 424401) or FOXP3 Fix/Perm buffer set (BioLegend, 421403) and analysed. MHC class I levels of vehicle- or recombinant mouse IFNγ-exposed (BioLegend, 714006)

(19) α-GalCer-LNP ehanced mRNA delivery and activates natural killer T cells for superior tumor immunotherapyMaterials Today BioJune 1, 2025Kexin Zhang, Zhen Xu, Zexiu Xiao, Fei Cao, Jing Wang, Yuandong Xu, Winson M.J. Ma, Sihui Long, Gao-Feng Zha(e.g., PLZF) were detected using Cyto-Fast Fix/Perm (BioLegend 750000135) and FoxP3 Fix/Perm (BioLegend 421403) kits, respectively, after surface staining, followed by intracellular/intranuclear antibody incubation

(20) LncRNA TCL6 regulates miR-876–5p/MYL2 axis to suppress breast cancer progressionTranslational OncologyMarch 1, 2025YaoBang Liu, Hong Li, DaHai Chai, Bin Lian, ZhengYang Bai, YaLi Gao, JinPing Licytokine staining. For nuclear protein staining, the cells were resuspended in 1x FOXP3 Fix/Perm buffer (421403, Biolegend, USA), mixed, and incubated at room temperature for 20 min. The cells were then resuspended

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FOXP3 Fix/Perm Buffer Set from BioLegend

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FOXP3 Fix/Perm Buffer Set from BioLegend

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