Nuclear extract from HeLa cells from Enzo Life Sciences, Inc.

Description

Prepared by high salt extraction of HeLa nuclei (human cervical cancer cell line), this extract is a rich source of HDAC activity, useful for inhibitor screening or immunoprecipitation of HDAC containing complexes. Typically, 0.5µl or less per well is sufficient for assay with the HDAC FLUOR DE LYS® fluorometric activity assay (Prod. No. BML-AK500)

Product Specs
ItemNuclear extract from HeLa cells
CompanyEnzo Life Sciences, Inc.
Price $104.00Supplier Page View Company Product Page
Catalog NumberBML-KI140-0100
Quantity100 µl
FormatLiquid. In 0.1M KCl, 20mM HEPES/NaOH, pH 7.9, 20% (v/v) glycerol, 0.2mM EDTA, 0.5mM DTT, 0.5mM PMSF
TypeAccessory
Molecule NameNuclear extract from HeLa cells

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Enzo Life Sciences, Inc.

10 Executive Blvd.

Farmingdale, NY 11735

United States

Phone: 800-942-0430
Fax: 610-941-9252

Company Profile

Website: www.enzo.com

(1) Histone deacetylase enzyme activity is not the universal anticancer target of HDAC inhibitorsSignal Transduct Target TherJune 5, 2026Chaitra Rai, Hang Ruan, Xue Li, Wenbo Li, Hyun-Hwan Jeong, Chengchuang Song, Panpan Liu, Yingjie Chang, Hao Fang, Udhaya Kumar. S, et al.in a 96-well black microplate, followed by 10 μL of pre-chilled diluted HeLa nuclear extract (Enzo, BML-KI140-0100). The mixture was pre-incubated at 4 °C for 5 min. Subsequently, 40 μL of the substrate (Boc-Lys(Ac)-AMC,

(2) Epigenetic Histone Deacetylases Activity Assay in the Brain and Peripheral Organ TissuesCurr ProtocJune 11, 2025Doodipala Samba Reddy, Hudson Boyd, Vinitha Karnati, Joon Kim, Sreevidhya Ramakrishnan, Xin Wuextract with a high known HDAC activity. For this protocol, we used HeLa cells (Enzo Life Sciences, BML‐KI140‐0100) for inhibitor screening.Add the reagents in the following order: assay buffer, inhibitor, and enzyme.

(3) Synthesis and biological evaluation of ortho-phenyl phenylhydroxamic acids containing phenothiazine with improved selectivity for class IIa histone deacetylasesJournal of enzyme inhibition and medicinal chemistryDecember 1, 2024Hsu KC, Huang YY, Chu JC, Huang YW, Hu JL, Lin TE, Yen SC, Weng JR, Huang WJ.reader (TECAN, Männedorf, Switzerland) was used for absorbance analysis. The HeLa nuclear extract (BML-KI140-0100) used in the enzyme inhibitory assay was purchased from Enzo Life Sciences. Unless otherwise mentioned,

(4) Measuring Histone Deacetylase Inhibition in the BrainCurrent protocols in pharmacologyJune 1, 2018Reddy DS, Wu X, Golub VM, Dashwood WM, Dashwood RH.tebu-bio) as a reference for standard HDAC activity, as can HeLa cell nuclear extract (Enzo Life Sciences, BML-KI140-0100) which can be used for inhibitor screening or immunoprecipitation of HDAC containing complexes (Rajendran

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Nuclear extract from HeLa cells from Enzo Life Sciences, Inc.

Description

Enzo Life Sciences, Inc.

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Nuclear extract from HeLa cells from Enzo Life Sciences, Inc.

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