MojoSort™ Buffer (5X) from BioLegend

Product Specs
ItemMojoSort™ Buffer (5X)
CompanyBioLegend
Price $66.00Supplier Page View Company Product Page
Catalog Number480017
Quantity250 mL
ApplicationsCell Separation (MojoSort™, ) - Quality tested

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) ULK1 drives NDP52-mediated selective autophagic degradation of MHC-I to promote immune evasion in HPV-positive head and neck cancerbioRxivMarch 20, 2026Lexi Vu, Nicholas S. Giacobbi, Mohamed I. Khalil, Canchai Yang, William J. Eckerman, Evelyn Gomez Recinos, Johnathon D. Garber, Hyomin Son, Prerna Chahal, Dara M. Villa, et al.was centrifuged at 350 × g for 10 min. Splenocytes were resuspended in 1X MojoSort Buffer (BioLegend #480017). CD8+ T cells were isolated from 1×108 splenocytes using the MojoSort Mouse CD8 T Cell Isolation Kit

(2) 5xFAD-NSG mice: a preclinical Alzheimer model for human cell therapy studiesbioRxivMarch 5, 2026David J Graber, Marie-Louise Sentman, W. James Cook, Charles L Sentmanliquid N2 and then thawed in cold XVI-VO-15 media, and then rinsed in cold MojoSort Buffer (Biolegend 480017, San Diego, CA, USA). Cells were labeled with anti-CD25-PE (1/50, Biolegend 356103) and then with anti-PE

(3) Extracellular vesicle-associated PD-L1 induces CD4+ T cell dysfunction and contributes to immunosuppression in sepsisCell Communication and SignalingFebruary 10, 2026Junhong Huang, Yuanyuan Xia, Yingqin Zhang, Chunmei Zhang, Zhizai Chen, Xun Hou, Zheng Wang, Bei Hu, Hongke Zeng, Xin Li, et al.mixed thoroughly, and incubated on ice for a further 15 min. Subsequently, 2.5 mL MojoSort™ buffer (#480017, Biolegend) was gently added along the wall of the tube. After placing on the separation magnet for

(4) PCID2 is essential for spermatogonial differentiation by regulating alternative splicingCellular and Molecular Life SciencesJanuary 13, 2026Feiyin Zhu, Ying Zhang, Yu Xi, Chenjia Gong, Yanlin Tang, Yidong Chen, Liying Yan, Jie Qiao, Qiang Liuthe final wash of testicular cells, cells were resuspended in 4 mL of 1× MojoSort™ Buffer (BioLegend, 480017). The cells were then filtered with a 70 μm cell strainer, centrifuged at 300 ×g for 5 min, and resuspended

(5) A Novel Image‐Based Approach for Analyzing Neutrophil Chemotaxis Using a Boyden Chamber AssayCurrent ProtocolsNovember 19, 2025Mohammad Umar, Gurpreet Kaur AulakhwellPrepare fresh before every experiment Can be prepared in‐lab or purchased from BioLegend (cat. no. 480017). Neutrophil recommended medium (NRM) 1 mM EDTA2% fetal bovine serum (FBS)1× PBS without Ca2+ and

(6) In vitro characterization of cellular responses elicited by endosomal TLR agonists encapsulated in Qβ virus-like particlesBMC ImmunologyNovember 17, 2025M. M. Hasibuzzaman, Briana Ibarra, Ishrat Nourin Khan, Thomas Craig Meagher, Caitlin D. Lemke-Miltner, George J. Weiner, Andrean L. Simonsmanufacturer’s protocol. Briefly, unfractionated PBMCs were resuspended in Mojoshrt Buffer (BioLegend, #480017) then incubated with a cocktail of antibody-conjugated magnetic beads. Magnetically labeled cells were

(7) Defects in anaplerotic metabolism sensitize Staphylococcus aureus small colony variants to bicarbonateMicrobiology SpectrumOctober 7, 2025Asif Iqbal, Zannatul H. Tumpa, Wyatt W. Wittliff, Bennett J. Blank, Basel H. Abuaita, William N. Beaversstrainer. Cells were collected by centrifugation and resuspended in MojoSort buffer (BioLegend, Cat. 480017). Neutrophils were purified by MojoSort mouse neutrophil isolation kit according to the manufacturer’s

(8) Integrin CD103 expression in naive CD8+ T cells promotes cytokine-driven acquisition of memory phenotype and effector functionImmunitySeptember 15, 2025Can Li, Davinna L. Ligons, Dominic Lanasa, Hilary R. Keller, Megan A. Luckey, Brian Capaldo, Daoud Meerzaman, Joo-Young Park, Jung-Hyun Parkdiluent,eBioscience,Cat#: 00-5223-56 IC fixation buffer,eBioscience,Cat#: 00-8222-49 MojoSort Buffer (5X),BioLegend,Cat#: 480017 MojoSort Mouse anti-PE Nanobeads,BioLegend,Cat#: 480080 Permeabilization buffer (10X),eBioscience,Cat#:

(9) Human CAR Tregs Targeting SOD1 and Expressing BDNF Reduce Inflammation and Delay Disease in G93A hSOD1-NSG MiceCellsAugust 26, 2025David J. Graber, W. James Cook, Marie-Louise Sentman, Joana M. Murad-Mabaera, Elijah W. Stommel, Charles L. SentmanCD4+ T cells were thawed in cold XVIVO-15 media and then rinsed in cold MojoSort Buffer (Biolegend 480017, San Diego, CA, USA). Cells were labeled with anti-CD25-PE (1/50, Biolegend 356103) and then with anti-PE

(10) Protocol to study juxtacrine signaling in human vascular cells using a fluorescence labeling co-culture approachSTAR protocolsJune 20, 2025Herrera JL, Komatsu M.(PM),ScienCell,1201 Endothelial cell growth basal medium phenol red free,Lonza,CC-3129 Mojo sort buffer (FACS),BioLegend,480017 4-well chamber glass slide with cover,Lab-Tek,154526 Cytoseal 60,Epredia,8310-4 Fluoromount-G,SouthernBiotech,0100-01 "4′,6-diamidino-2-phenylindole

(11) Protocol for CRISPR-mediated deletion of cis-regulatory element in murine Th17 cells for in vivo assessment of effector functionSTAR ProtocolsJune 20, 2025Xiancai Zhong, Hongmin Wu, Guanpeng Wang, Zuoming SunB01-03 GolgiStop protein transport inhibitor,BD Biosciences,Cat# BDB554724 MojoSort Buffer (5X),BioLegend,Cat# 480017 Dulbecco’s modified Eagle’s medium,Corning,Cat# 15-013-CV Fetal bovine serum (FBS),Corning,Cat# 35-011-CV HEPES,Corning,Cat#

(12) An epitope-directed mRNA vaccine inhibits tumor metastasis through the blockade of MICA/B α1/2 sheddingCell Reports MedicineMarch 18, 2025Rui Wang, Jingni Wu, Yifeng Lin, Yufei Xiao, Bin Yang, Sheng Yao, Tianhui Pan, Zhixuan Fu, Shuyu Li, Caihua Wang, et al.incubating on ice for another 15 min, and then resuspended in 2.5 mL of MojoSort Buffer (BioLegend, 480017). Place the tube in a magnet for 5 min, and the sorted cells was poured out and collected. Isolation

(13) Mapping cell diversity and dynamics in inflammatory temporomandibular joint osteoarthritis with pain at single-cell resolutionJCI InsightFebruary 10, 2025Supawadee Jariyasakulroj, Yang Shu, Ziying Lin, Jingyi Chen, Qing Chang, Pao-Fen Ko, Jian-Fu Chenthe cell dissociation step were washed and resuspended with cold MojoSort Buffer (BioLegend, catalog 480017). Cells were pelleted via centrifugation at 300g for 5 minutes at 4°C, and the supernatant was discarded.

(14) CryoSCAPE: Scalable immune profiling using cryopreserved whole blood for multi-omic single cell and functional assaysJournal of translational medicineJanuary 3, 2025Heubeck AT, Phalen C, Kaul N, Wittig PJ, Garber J, Weiss M, Genge PC, Thomson Z, Gustafson C, Reading J, et al.

(15) RNA Shielding of p65 Is Required to Potentiate Oncogenic Inflammation in TET2-Mutated Clonal HematopoiesisCancer discoveryDecember 2, 2024Ben-Crentsil NA, Mohammed Ismail W, Balasis ME, Newman H, Quintana A, Binder M, Kruer T, Neupane S, Ferrall-Fairbanks MC, Fernandez J, et al.BMT recipients were negatively selected using the MojoSort Mouse CD45.1 Selection Kit (BioLegend Cat# 480017) and then mixed with fresh BoyJ CD45.1 competitor BM cells in a 4:1 ratio. 5 million total cells were

(16) Reformed islets: a long-term primary cell platform for exploring mouse and human islet biologyCell Death DiscoveryNovember 23, 2024N. Haq, K. W. Toczyska, M. E. Wilson, M. Jacobs, Min Zhao, Y. Lei, Z. Shen, J. A. Pearson, S. J. Persaud, T. J. Pullen, et al.MojoSort Mouse CD8 T-cell isolation kit (Cat# 480043, BioLegend, San Diego, CA, USA) and MojoSort buffer (Cat# 480017, BioLegend). Cell Biolabs’ CytoSelect™ cell migration assay from Cell Biolabs and EZCellTM cell migration/chemotaxis

(17) Probiotics and their metabolite spermidine enhance IFN-γ(+)CD4(+) T cell immunity to inhibit hepatitis B virusCell reports. MedicineNovember 19, 2024Wang T, Fan Y, Tan S, Wang Z, Li M, Guo X, Yu X, Lin Q, Song X, Xu L, et al.N8090 Ampicilin,Solarbio,Cat# A8180 Metronidazole,Solarbio,Cat# M8060 MojoSort™ buffer (5×),BioLegend,Cat# 480017 IC Fixation Buffer,eBioscience,Cat# 00-8222-49 Permeabilization Buffer 10×,eBioscience,Cat# 00-8333-56 Intracellular

(18) CRISPR screens and lectin microarrays identify high mannose N-glycan regulatorsNature CommunicationsNovember 18, 2024C. Kimberly Tsui, Nicholas Twells, Jenni Durieux, Emma Doan, Jacqueline Woo, Noosha Khosrojerdi, Janiya Brooks, Ayodeji Kulepa, Brant Webster, Lara K. Mahal, et al.cells for 1 hour at 4 °C with rotation. Cells were then washed 2x with cold MojoSort buffer (BioLegend, 480017), and resuspended in MojoSort buffer. Cells were then placed on magnet and allowed to separate for 10

(19) Eosinophils mitigate intestinal fibrosis while promoting inflammation in a chronic DSS colitis model and co-culture model with fibroblastsScientific reportsNovember 7, 2024Jacobs I, Deleu S, Ke BJ, Cremer J, Dilissen E, De Hertogh G, Martens T, Vanden Berghe P, Matteoli G, Vermeire S, et al.which the cells were washed and centrifuged for 5 min at 300 g with 1X MojoSort buffer (Biolegend, 480017). Next, the cells were incubated for 15 min on ice in an appropriate amount of MojoSort™ Streptavidin Nanobeads

(20) IRF3 inhibits inflammatory signaling pathways in macrophages to prevent viral pathogenesisScience AdvancesAugust 9, 2024Sukanya Chakravarty, Merina Varghese, Shumin Fan, Roger Travis Taylor, Ritu Chakravarti, Saurabh Chattopadhyayfilter. These cells were then centrifuged, and the pellet was resuspended in MojoSort buffer (BioLegend, #480017) for cell counting. Equal number of cells isolated from each lung was then incubated with anti-F4/80

Be the First to Write a Review!

Four Key Considerations When Performing Magnetic Cell Separation
February 02, 2016
The separation of specific cells within a complex cell mixture is a challenge that researchers face on a daily basis. Three major techniques have emerged as most widely used: centrifugation-based, fluorescence-activated ... read more

MojoSort™ Buffer (5X) from BioLegend

Description

BioLegend

Citations (45)

Reviews

Bench Tips

Four Key Considerations When Performing Magnetic Cell Separation

MojoSort™ Buffer (5X) from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map