ViaFect Transfection Reagent from Promega

Product Specs
ItemViaFect Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE4982
Quantity2 x 0.75ml
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) High-efficiency transfection of Acanthamoeba castellanii using a cationic polymerCell Reports MethodsFebruary 23, 2026Anaísa B. Moreno, Viktor Ek, Jens Eriksson, Mikael E. Sellin, Lionel Guy41003 SuperFect Transfection Reagent,QIAGEN,Cat# 301305 ViaFect Transfection Reagent,Promega,Cat# E4981 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, NucleoSpin Tissue

(2) Hypokalaemia and bradycardia unmask the loss-of-function phenotype of a Brugada Syndrome SCN5A mutationEuropaceFebruary 3, 2026Anthony Frosio, Procolo Marchese, Giorgia Bertoli, David Molla, Martina Arici, Chiara Bartolucci, Chiara Piantoni, Giulia Guidi, Claudia Bazzini, Patrizia Benzoni, et al.#P4458) at a controlled temperature of 37°C and 5% CO2. The ViaFect™ transfection reagent (Promega, #E4981) was used to transiently transfect HEK293 cells with pCI Mammalian Expression Vector (GenBank U47119.2)

(3) Mechanical force locally damages, remodels, and stabilizes the lattice of spindle microtubulesCurrent BiologyFebruary 2, 2026Caleb J. Rux, Megan K. Chong, Valerie Myers, Nathan H. Cho, Sophie DumontCY-SC002 Janelia Fluor 646 HaloTag ligand,Promega,Cat# GA1120 ViaFect™ Transfection Reagent,Promega,Cat# E4982 Deposited data,Deposited data,Deposited data Raw and analyzed data,This study; Mendeley Data,Mendeley

(4) Single-cell analysis identifies BASP1 as a driver of drug resistance and cell plasticity in oral squamous cell carcinomaJournal of Biological ChemistryFebruary 1, 2026Abinash Behera, Sudeshna Datta, Sibasish Mohanty, Pallavi Mohapatra, Shamima Azma Ansari, Sreeparna Podder, Rachna Rath, Dillip Kumar Muduly, Rajeeb K. Swain, Sunil K. Raghav, et al.pCMV6-LIN7A (Myc-DDK-tagged) (Origene, Cat No# RC221902) using ViaFect transfection reagent (Promega, Cat No# E4982) separately at approximately 40 to 60% confluency. Successful transfection was confirmed by immunoblotting

(5) Assessing PARP trapping dynamics in ovarian cancer using a CRISPR-engineered FRET biosensorCell Reports MethodsJanuary 26, 2026Daniel Marks, Edwin Garcia, Sunil Kumar, Katie Tyson, Caroline Koch, Aleksandar P. Ivanov, Joshua B. Edel, Hasan B. Mirza, William Flanagan, Christopher Dunsby, et al.6-well plates in antibiotic-free medium and transfected with 1 μg of each plasmid using Viafect (Promega E4981). Fluorescence positive cells were isolated after 24–48h using FACS, and single cell colonies were expanded. BRCA1

(6) Differential Regulatory Effects of Cannabinoids and Vitamin E Analogs on Cellular Lipid Homeostasis and Inflammation in Human MacrophagesAntioxidants (Basel)January 16, 2026Mengrui Li, Sapna Deo, Sylvia Daunert, Jean-Marc Zinggwere transfected using ViaFect™ Transfection Reagent according to the manufacturer’s protocol (Promega E4981). Cells (1.5 × 105 cells/well) were seeded in 6-well plates 24 h before transfection. Plasmids pRL-TK

(7) LOTUS-domain proteins activate Vasa to drive germ granule assemblybioRxivJanuary 11, 2026Ian F. Price, Chin Wang, Wen Tangtransfected with 2 μg of TDRD5::GFP and/or DDX4::mCherry expression plasmids using ViaFect (Promega, E4981) according to the manufacturer’s instructions. Image analysis Quantification of condensed GLH-1 fraction. A

(8) Fentanyl blockade of K + channels contributes to wooden chest syndromeThe Journal of PhysiologyJanuary 1, 2026Aguan D. Wei, Nicholas J. Burgraff, Luiz M. Oliveira, Thiago S. Moreira, Jan‐Marino Ramirezexpression studies. Plasmid constructs were acutely transfected into HEK293 cells using Viafect reagent (E4981; Promega, Madison, WI, USA), following the manufacturer’s protocol. In brief, HEK293 cells were prepared

(9) Synchronization of the segmentation clock using synthetic cell–cell signalingGenes & DevelopmentJanuary 1, 2026Akihiro Isomura, Daisuke Asanuma, Ryoichiro KageyamapT2A and 0.5 µg of pCAGGS-mT2TP plasmids were transfected using ViaFect transfection reagent (Promega E4981) into cells cultured in a 12 well plate with a density of 2 × 105 cells. Transfected cells were expanded

(10) ITM2B Truncation Promotes Migrasome Formation to Accelerate Renal Cell Carcinoma GrowthAdvanced ScienceJanuary 1, 2026Qi‐tao Chen, Qiao‐ling Huang, Ming‐zhi Han, Xue‐hui Hong, De‐yi Feng, Lu‐ming Yao, Wen‐bin Hong, Yue Chen, Ya‐ying Huang, Hang‐zi Chen, et al.equilibrated with 5% CO2 at 37 °C. Transfections were performed using ViaFect Transfection Reagent (#E4982, Promega) for 786‐O cells according to the manufacturer's instructions, and the calcium–phosphate precipitation

(11) GATOR1 complex controls cisplatin sensitivityCell Death & DiseaseDecember 30, 2025Zhenrui Pan, Hanxiao Zhang, Xia Xiao, Catherine Brenner, Svetlana Dokudovskayaplasmids encoding Flag-NPRL2, Flag-NPRL3, or Flag-DEPDC5 using ViaFect™ Transfection Reagent (Promega, E4981) according to the manufacturer’s instructions. For each well, 100 ng plasmid DNA was mixed with ViaFect

(12) A bifunctional optical reporter for tracking estrogen response dynamics in neuronsbioRxivDecember 14, 2025Alexandra L. Cara, Eartha Mae Guthman, Aarya Mishra, Alexanne L. Hall, Sae Yokoyama, Norma P. Sandoval, Ian Gregg, Krisha Aghi, Kiran K. Soma, Stephanie M. Correa, et al.receptor and reporter DNA were transfected in a 1:3 ratio using the ViaFect Transfection Reagent (Promega E4981, Madison, WI) per supplier’s recommendations. Following another 24 h, hormone (17β-estradiol (E2) or

(13) Genetic evidence for PARP1 trapping as a driver of PARP inhibitor efficacy in BRCA mutant cancer cellsNucleic Acids ResDecember 11, 2025Jonathan Ribeiro, Kjetil Hansen, Lotte van Beek, Christopher Stubbs, Joan Frigola, Sara Talbot, Sabrina Bentouati, James Hall, Paul W G Wijnhoven, Josh Armenia, et al.transfected with pPARP1 (WT or L777P) -IRES-H2B-GFP vectors using the ViaFect Transfection Reagent (Promega, #E4981) and following the manufacturer’s instructions. Twenty-four hours post-transfection, media was changed.

(14) Repurposing clinically safe drugs for DNA repair pathway choice in CRISPR genome editing and synthetic lethalityNat CommunDecember 10, 2025Dominik Macak, Philipp Kanis, Stephan RiesenbergMMEJ substrate and 20 ng pGL4.53 (MMEJ). Transfection was performed using ViaFect reagent (Promega, E4981) at a ratio of 4 μl reagent per 1 μg of total DNA. For each well, the DNA mixture and the ViaFect reagent

(15) Regulated TRESLIN-MTBP loading governs initiation zones and replication timing in human DNA replicationNat CommunDecember 2, 2025Xiaoxuan Zhu, Atabek Bektash, Yuki Hatoyama, Sachiko Muramatsu, Shin-Ya Isobe, Chikashi Obuse, Atsushi Toyoda, Yasukazu Daigaku, Chun-Long Chen, Masato T. Kanemakiin this study are listed in Supplementary Table 2. Cells were transfected using ViaFect (Promega, #E4982) with CRISPR and donor plasmids to tag endogenous proteins, as described previously91. All guide-RNA

(16) Impaired cAMP-PKA-CREB1 signalling drives mitochondrial dysfunction in skeletal muscle during cancer cachexiaNature metabolismDecember 1, 2025Angelino E, Bodo L, Sartori R, Malacarne V, D'Anna B, Formaggio N, Barua S, Raiteri T, Lauria A, Reano S, et al.transfected with the ICUE3 Epac2-cAMP plasmid36. For transfection, ViaFect transfection reagent (Promega; E4982) was used at a 4:1 ratio (transfection reagent:DNA), following the manufacturer’s instructions. After

(17) Nanoscale architecture and dynamics of CaV1.3 channel clusters in cardiac myocytes revealed by single channel nanoscopyJournal of Molecular and Cellular Cardiology PlusDecember 1, 2025Niko Schwenzer, Roman Tsukanov, Tobias Kohl, Samrat Basak, Izzatullo Sobitov, Fitzwilliam Seibertz, Rohan Kapoor, Niels Voigt, Jörg Enderlein, Stephan E. Lehnart[41]. Cells were transfected the following day using 0.6–1 μg of plasmid and Viafect reagent (Promega E4981, 6 μl/μg plasmid). The medium was exchanged the following day to regular culture medium. Cells were

(18) Combination therapy overcomes secondary PARPi resistance in ATM-deficient prostate cancernpj Precision OncologyNovember 26, 2025Sara Arce-Gallego, Victor Esquefa, Heura Domenech, Andrei Salca, Daniel Aguilar, Teresa Casals, Gisela Mir, Sara Simonetti, Anna Oliveira, Lara de Llobet, et al.transfected after 24 h with 3 μg of the pHPRT-DR-GFP vector (Addgene 26475) using Viafect (Promega E4981); 48 h later, puromycin (1 μg/ml) was added to the media for 4 days. Surviving cells (DR-GFP stable

(19) Tau seeding in neurons enabled by transient endolysosomal perforations are confined within endolysosomesbioRxivNovember 5, 2025Anwesha Sanyal, Gustavo Scanavachi, Elliott Somerville, Beren Aylan, Jose Inacio Costa-Filho, Forest Brooks, John R. Dickson, Bradley T. Hyman, Tom Kirchhausenwell in 8-well chambered coverslips (Cellvis, C8-1.5H-N). We transfected cells with ViaFect (Promega, E4981) according to the manufacturer’s instructions. For each well, we mixed 0.2 μg plasmid DNA with 0.6 μL

(20) Reduced Expression of MTSS1 Increases Sarcomere Number and Improves Contractility in Select Forms of Monogenic DCMJACC Basic Transl SciOctober 17, 2025Hannah Kleppe, Anastasia Budan, Luke Zhang, Marie Majetic, Reva Shenwai, Alan R. Levinson, Olga Cisne-Thompson, Bernardo Zepeda, Charles E. MacKay, Farshad Farshidfar, et al.advance. After 96 hours, cells were transfected with 2.5 μg of MTSS1-GFP construct using ViaFect (Promega E4981) reagent according to the manufacturer’s protocol. Medium with ViaFect and DNA was removed after 24

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6/17/2019Easy-to-Use Transfection Reagent with Good Results from Promega Wei Yang SitHighly recommended transfection reagent for those who intend to carry out transfection.