ViaFect Transfection Reagent from Promega

Product Specs
ItemViaFect Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE4982
Quantity2 x 0.75ml
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Characterization of Electrophysiological and Transcriptomic Alterations in Patient-Derived Neurons from CHAMP1 SyndromebioRxivJuly 29, 2026Dailey Nettles, Christina Stanton, Zach Hunter, Bryan Granger, Elizabeth Wallace, Andrew Lutsky, Suganya Subramanian, Meris Privette, Lori McMahon, Stefano Bertoplasmid #62988). Plasmids were transfected into iPSCs using ViaFect Transfection Reagent (Promega, E4981). Twenty-four hours after transfection, cells were cultured in mTeSR medium supplemented with zFGF and

(2) Epigenetic landscape of synthetic human centromere core regions: CENP-A assembly and euchromatic modifications interdependently antagonize heterochromatin accumulationNucleic Acids ResearchJune 30, 2026Junichiro Ohzeki, Akiko Watanabe, Jia Xian Lee, Chiharu Minami, Kazuto Kugou, Kyotaro Yamazaki, Kenta Shirasawa, Sachiko Isobe, Yusuke Endo, Yasuhiro Kazukithe cells grown at approximately 80% confluency, BAC plasmids were transfected using 8 µl of ViaFect (E4981, Promega) diluted in 100 µl of the Opti-Mem I (31985062, gibco). After 24 h incubation, cells were trypsinized

(3) A Comprehensive HLA-DR4 MHC Class II Tetramer Platform for the Detection and Functional Validation of Post-Translational Modification NeoantigensInternational Journal of Molecular SciencesJune 23, 2026Henghui Li, Jingyao Li, Ying Wang, Hongyan Ma, Fen Tang, Liang Chento equilibrate at room temperature for 5 min, after which 12 μL ViaFect™ Transfection Reagent (Cat. E4981, Promega (Beijing) Biotech, Beijing, China) was added, and the mixture was incubated at room temperature

(4) A cell-impermeable kinase inhibitor uncovers outside-in signaling pathways that promote HIV-1 infectionJournal of VirologyJune 23, 2026Kelsey Vinzant, Natalia Cheshenko, Heng Pan, Ronald Cutler, Joshua N. Buckler, Andreas Luxenburger, Lawrence D. Harris, Jeffrey R. Johnson, Steven C. Almo, Betsy C. Heroldpseudoviruses were produced by transfecting HEK293T in T150s using the ViaFect Transfection Reagent (Promega, E4981). EGFP labeled HIV-1 was produced by co-transfecting 60 µg pNL4-3BaL p21-85 (NIH HIV Reagent Program,

(5) Consequences of human genetic variations in KIAA0319L, encoding adeno-associated virus receptor, on AAV-mediated gene transferMolecular Therapy AdvancesJune 11, 2026Jenifer M. Vasquez, Hailey L. Mackey, Mahboubeh R. Rostami, Philip L. Leopold, Ronald G. CrystalAAVR, nonsense variants of AAVR, or predicted deleterious missense variants of AAVR using Viafect (E4981, Promega, Madison, WI) at a 3:1 ratio resulting in a final solution of 10 ng/μL. From this solution,

(6) Light- and chemical-induced ciliary signaling governs dorsal/ventral regionalization of human telencephalic organoidsNature CommunicationsMay 22, 2026Issei S. Shimada, Akari Goto, Yutaka Hashimoto, Hajime Inoue, Takuto Sugawara, Tomohiro Doura, Tsubasa Fujita, Toshiaki Iwata, Riko Shimmoto, Hiroshi Takase, et al.pCDMd1-GPR161-EGFP, pCDMd1-GPR161HHC-EGFP, pCDMd1 vector control, and pCAGGS-Gα15) using ViaFect (Promega, E4981) or Lipofectamine 3000 (Thermo Fisher Scientific, L3000001) according to the manufacturer’s instructions.

(7) De novo design of a macrocycle-induced dimerization system for cellular controlNature CommunicationsMay 18, 2026Stephanie Hanna, Patrick J. Salveson, Basile Wicky, Madison A. Kennedy, Derrick R. Hicks, Carolina Moller, Suna Cheng, Xinting Li, Mohamad Abedi, Brian Coventry, et al.(Thermo Fisher 31985070) to a concentration of 6.25 ng/µl per construct. Viafect transfection reagent (Promega E4981) at 3:1 DNA: transfection reagent was added, and the solution was mixed by pipetting up and down. The

(8) Long-lived mammals contain more phosphorylation sites in the SIRT6 C-terminus that enhance PARP1 interaction and resistance to oxidative stressbioRxivApril 30, 2026Jonathan Gigas, Michael E. Meadow, Jing Guo, Catherine Lan, Eric Hillpot, John C. Martinez, Gregory Tombline, Philip Bellomio, Valeria Rivera Almodovar, Kevin A. Welle, et al.into pFN21A vectors (Promega Cat#G2821) and transfected into HEK293T cells using Viafect (Promega Cat#E4982) at 25 μg DNA/15cm plate, with two plates per condition. Cells were harvested 72 hours after transfection

(9) Zinc-redox crosstalk regulates proteostasis in the endoplasmic reticulumNature CommunicationsApril 22, 2026Yuta Amagai, Chihiro Arai, Wakana Yamamoto, Satoshi Watanabe, Toshiyuki Kowada, Roberto Sitia, Jun Hoseki, Shin Mizukami, Masaki Matsumoto, Kenji Inabatransfected using FuGENE-HD transfection reagent (E2311, Promega) or ViaFect transfection reagent (E4981, Promega), while siRNA was transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific). RNA interference

(10) TREX2 component PCID2 scaffolds alternative SAC3-based subcomplexes with distinct RNA processing and export functionbioRxivApril 17, 2026Vasilisa Aksenova, Elizabeth Giordano, Caroline Esnault-Petrov, Alexei Arnaoutov, Mary Dassoand 500 ng of mixture of gRNA plasmids at a ratio 1:1 using ViaFect transfection reagent (Promega, E4981) according to the manufacturer’s instruction. Seventy-two hours after transfection, cells were seeded

(11) The gamma-Tubulin Ring Complex promotes mitotic spindle integrity and acts as a microtubule minus-end cap during mitosisbioRxivApril 15, 2026Reham Aljumaah, Elizabeth A Turcotte, Sanjana Sundararajan, Vasilisa Aksenova, Alexei Arnaoutov, Mary Dassotransfected with the donor and guide plasmids (1000 ng total) in a 1:1 proportion using Viafect (Promega, E4981) reagent according to manufacturer’s instructions. After transfection (72 hrs), cells were seeded on

(12) Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregationFEBS Open BioMarch 30, 2026Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, et al.instructions. Transfection efficiency Transfections were performed using ViaFect Transfection Reagent (Catalog #E4981; Promega), which is optimized for HEK cells. Plasmid DNA encoding BFP (T.gonfio) or a GFP plasmid of

(13) Structure-function relationship of alpha-synuclein fibrillar polymorphs derived from distinct synucleinopathiesMolecular Systems BiologyMarch 11, 2026Tetiana Serdiuk, Virginie Redeker, Jimmy Savistchenko, Sandesh Neupane, Walther Haenseler, Yanick Fleischmann, Viviane Reber, Sabrina Keller, Cinzia Tiberi, Ruxandra Bachmann-Gagescu, et al.delivery of the gRNA plasmids, transfection was performed using the ViaFect™ Transfection Reagent (#E4981, Promega). Following transfection, the cells were selected with Puromycin. 48 h post-selection patient

(14) High-efficiency transfection of Acanthamoeba castellanii using a cationic polymerCell Reports MethodsFebruary 23, 2026Anaísa B. Moreno, Viktor Ek, Jens Eriksson, Mikael E. Sellin, Lionel Guy41003 SuperFect Transfection Reagent,QIAGEN,Cat# 301305 ViaFect Transfection Reagent,Promega,Cat# E4981 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, NucleoSpin Tissue

(15) Hypokalaemia and bradycardia unmask the loss-of-function phenotype of a Brugada Syndrome SCN5A mutationEuropaceFebruary 3, 2026Anthony Frosio, Procolo Marchese, Giorgia Bertoli, David Molla, Martina Arici, Chiara Bartolucci, Chiara Piantoni, Giulia Guidi, Claudia Bazzini, Patrizia Benzoni, et al.#P4458) at a controlled temperature of 37°C and 5% CO2. The ViaFect™ transfection reagent (Promega, #E4981) was used to transiently transfect HEK293 cells with pCI Mammalian Expression Vector (GenBank U47119.2)

(16) Mechanical force locally damages, remodels, and stabilizes the lattice of spindle microtubulesCurrent BiologyFebruary 2, 2026Caleb J. Rux, Megan K. Chong, Valerie Myers, Nathan H. Cho, Sophie DumontCY-SC002 Janelia Fluor 646 HaloTag ligand,Promega,Cat# GA1120 ViaFect™ Transfection Reagent,Promega,Cat# E4982 Deposited data,Deposited data,Deposited data Raw and analyzed data,This study; Mendeley Data,Mendeley

(17) Single-cell analysis identifies BASP1 as a driver of drug resistance and cell plasticity in oral squamous cell carcinomaJournal of Biological ChemistryFebruary 1, 2026Abinash Behera, Sudeshna Datta, Sibasish Mohanty, Pallavi Mohapatra, Shamima Azma Ansari, Sreeparna Podder, Rachna Rath, Dillip Kumar Muduly, Rajeeb K. Swain, Sunil K. Raghav, et al.pCMV6-LIN7A (Myc-DDK-tagged) (Origene, Cat No# RC221902) using ViaFect transfection reagent (Promega, Cat No# E4982) separately at approximately 40 to 60% confluency. Successful transfection was confirmed by immunoblotting

(18) Assessing PARP trapping dynamics in ovarian cancer using a CRISPR-engineered FRET biosensorCell Reports MethodsJanuary 26, 2026Daniel Marks, Edwin Garcia, Sunil Kumar, Katie Tyson, Caroline Koch, Aleksandar P. Ivanov, Joshua B. Edel, Hasan B. Mirza, William Flanagan, Christopher Dunsby, et al.6-well plates in antibiotic-free medium and transfected with 1 μg of each plasmid using Viafect (Promega E4981). Fluorescence positive cells were isolated after 24–48h using FACS, and single cell colonies were expanded. BRCA1

(19) Differential Regulatory Effects of Cannabinoids and Vitamin E Analogs on Cellular Lipid Homeostasis and Inflammation in Human MacrophagesAntioxidants (Basel)January 16, 2026Mengrui Li, Sapna Deo, Sylvia Daunert, Jean-Marc Zinggwere transfected using ViaFect™ Transfection Reagent according to the manufacturer’s protocol (Promega E4981). Cells (1.5 × 105 cells/well) were seeded in 6-well plates 24 h before transfection. Plasmids pRL-TK

(20) LOTUS-domain proteins activate Vasa to drive germ granule assemblybioRxivJanuary 11, 2026Ian F. Price, Chin Wang, Wen Tangtransfected with 2 μg of TDRD5::GFP and/or DDX4::mCherry expression plasmids using ViaFect (Promega, E4981) according to the manufacturer’s instructions. Image analysis Quantification of condensed GLH-1 fraction. A

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6/17/2019Easy-to-Use Transfection Reagent with Good Results from Promega Wei Yang SitHighly recommended transfection reagent for those who intend to carry out transfection.