ViaFect Transfection Reagent from Promega

Product Specs
ItemViaFect Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE4982
Quantity2 x 0.75ml
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Consequences of human genetic variations in KIAA0319L, encoding adeno-associated virus receptor, on AAV-mediated gene transferMolecular Therapy AdvancesJune 11, 2026Jenifer M. Vasquez, Hailey L. Mackey, Mahboubeh R. Rostami, Philip L. Leopold, Ronald G. CrystalAAVR, nonsense variants of AAVR, or predicted deleterious missense variants of AAVR using Viafect (E4981, Promega, Madison, WI) at a 3:1 ratio resulting in a final solution of 10 ng/μL. From this solution,

(2) Long-lived mammals contain more phosphorylation sites in the SIRT6 C-terminus that enhance PARP1 interaction and resistance to oxidative stressbioRxivApril 30, 2026Jonathan Gigas, Michael E. Meadow, Jing Guo, Catherine Lan, Eric Hillpot, John C. Martinez, Gregory Tombline, Philip Bellomio, Valeria Rivera Almodovar, Kevin A. Welle, et al.into pFN21A vectors (Promega Cat#G2821) and transfected into HEK293T cells using Viafect (Promega Cat#E4982) at 25 μg DNA/15cm plate, with two plates per condition. Cells were harvested 72 hours after transfection

(3) TREX2 component PCID2 scaffolds alternative SAC3-based subcomplexes with distinct RNA processing and export functionbioRxivApril 17, 2026Vasilisa Aksenova, Elizabeth Giordano, Caroline Esnault-Petrov, Alexei Arnaoutov, Mary Dassoand 500 ng of mixture of gRNA plasmids at a ratio 1:1 using ViaFect transfection reagent (Promega, E4981) according to the manufacturer’s instruction. Seventy-two hours after transfection, cells were seeded

(4) The gamma-Tubulin Ring Complex promotes mitotic spindle integrity and acts as a microtubule minus-end cap during mitosisbioRxivApril 15, 2026Reham Aljumaah, Elizabeth A Turcotte, Sanjana Sundararajan, Vasilisa Aksenova, Alexei Arnaoutov, Mary Dassotransfected with the donor and guide plasmids (1000 ng total) in a 1:1 proportion using Viafect (Promega, E4981) reagent according to manufacturer’s instructions. After transfection (72 hrs), cells were seeded on

(5) High-efficiency transfection of Acanthamoeba castellanii using a cationic polymerCell Reports MethodsFebruary 23, 2026Anaísa B. Moreno, Viktor Ek, Jens Eriksson, Mikael E. Sellin, Lionel Guy41003 SuperFect Transfection Reagent,QIAGEN,Cat# 301305 ViaFect Transfection Reagent,Promega,Cat# E4981 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, NucleoSpin Tissue

(6) Piezo1-mediated mechano-energetics regulate CAR T cell functionResearch SquareFebruary 10, 2026Weiqiang Chen, Ngoc Luu, Yifei Fang, Huishu Fang, Huishu Wang, Yujing Song, Xiangyi Chen, Xiangyi Fang, Junru Liao, Katsuo Kurabayashi, et al.Dr. Louis Hodgson’s laboratory at Albert Eistein College of using Viafect Transfection reagent (CAT#E4981, Promega) with cellular FRET/donor fluorescence emission ratio to delineate Rac1 activity patterns at

(7) Hypokalaemia and bradycardia unmask the loss-of-function phenotype of a Brugada Syndrome SCN5A mutationEuropaceFebruary 3, 2026Anthony Frosio, Procolo Marchese, Giorgia Bertoli, David Molla, Martina Arici, Chiara Bartolucci, Chiara Piantoni, Giulia Guidi, Claudia Bazzini, Patrizia Benzoni, et al.#P4458) at a controlled temperature of 37°C and 5% CO2. The ViaFect™ transfection reagent (Promega, #E4981) was used to transiently transfect HEK293 cells with pCI Mammalian Expression Vector (GenBank U47119.2)

(8) Mechanical force locally damages, remodels, and stabilizes the lattice of spindle microtubulesCurrent BiologyFebruary 2, 2026Caleb J. Rux, Megan K. Chong, Valerie Myers, Nathan H. Cho, Sophie DumontCY-SC002 Janelia Fluor 646 HaloTag ligand,Promega,Cat# GA1120 ViaFect™ Transfection Reagent,Promega,Cat# E4982 Deposited data,Deposited data,Deposited data Raw and analyzed data,This study; Mendeley Data,Mendeley

(9) Single-cell analysis identifies BASP1 as a driver of drug resistance and cell plasticity in oral squamous cell carcinomaJournal of Biological ChemistryFebruary 1, 2026Abinash Behera, Sudeshna Datta, Sibasish Mohanty, Pallavi Mohapatra, Shamima Azma Ansari, Sreeparna Podder, Rachna Rath, Dillip Kumar Muduly, Rajeeb K. Swain, Sunil K. Raghav, et al.pCMV6-LIN7A (Myc-DDK-tagged) (Origene, Cat No# RC221902) using ViaFect transfection reagent (Promega, Cat No# E4982) separately at approximately 40 to 60% confluency. Successful transfection was confirmed by immunoblotting

(10) Assessing PARP trapping dynamics in ovarian cancer using a CRISPR-engineered FRET biosensorCell Reports MethodsJanuary 26, 2026Daniel Marks, Edwin Garcia, Sunil Kumar, Katie Tyson, Caroline Koch, Aleksandar P. Ivanov, Joshua B. Edel, Hasan B. Mirza, William Flanagan, Christopher Dunsby, et al.6-well plates in antibiotic-free medium and transfected with 1 μg of each plasmid using Viafect (Promega E4981). Fluorescence positive cells were isolated after 24–48h using FACS, and single cell colonies were expanded. BRCA1

(11) Glypican-1 upregulation elicited in response to a cell-impermeable kinase inhibitor and its overexpression enhance HIV-1 infectionbioRxivJanuary 16, 2026Kelsey Vinzant, Natalia Cheshenko, Heng Pan, Ronald Cutler, Joshua N. Buckler, Andreas Luxenburger, Lawrence D. Harris, Jeffrey R. Johnson, Steven C. Almo, Betsy C. Heroldpseudoviruses were produced by transfecting HEK293T in T150s using the ViaFect Transfection Reagent (Promega, E4981). EGFP labeled HIV-1 was produced by co-transfecting 60 ug pNL4–3BaL p21–85 (NIH HIV Reagent Program,

(12) Differential Regulatory Effects of Cannabinoids and Vitamin E Analogs on Cellular Lipid Homeostasis and Inflammation in Human MacrophagesAntioxidants (Basel)January 16, 2026Mengrui Li, Sapna Deo, Sylvia Daunert, Jean-Marc Zinggwere transfected using ViaFect™ Transfection Reagent according to the manufacturer’s protocol (Promega E4981). Cells (1.5 × 105 cells/well) were seeded in 6-well plates 24 h before transfection. Plasmids pRL-TK

(13) LOTUS-domain proteins activate Vasa to drive germ granule assemblybioRxivJanuary 11, 2026Ian F. Price, Chin Wang, Wen Tangtransfected with 2 μg of TDRD5::GFP and/or DDX4::mCherry expression plasmids using ViaFect (Promega, E4981) according to the manufacturer’s instructions. Image analysis Quantification of condensed GLH-1 fraction. A

(14) Synchronization of the segmentation clock using synthetic cell–cell signalingGenes & DevelopmentJanuary 1, 2026Akihiro Isomura, Daisuke Asanuma, Ryoichiro KageyamapT2A and 0.5 µg of pCAGGS-mT2TP plasmids were transfected using ViaFect transfection reagent (Promega E4981) into cells cultured in a 12 well plate with a density of 2 × 105 cells. Transfected cells were expanded

(15) ITM2B Truncation Promotes Migrasome Formation to Accelerate Renal Cell Carcinoma GrowthAdvanced ScienceJanuary 1, 2026Qi‐tao Chen, Qiao‐ling Huang, Ming‐zhi Han, Xue‐hui Hong, De‐yi Feng, Lu‐ming Yao, Wen‐bin Hong, Yue Chen, Ya‐ying Huang, Hang‐zi Chen, et al.equilibrated with 5% CO2 at 37 °C. Transfections were performed using ViaFect Transfection Reagent (#E4982, Promega) for 786‐O cells according to the manufacturer's instructions, and the calcium–phosphate precipitation

(16) Fentanyl blockade of K + channels contributes to wooden chest syndromeThe Journal of PhysiologyJanuary 1, 2026Aguan D. Wei, Nicholas J. Burgraff, Luiz M. Oliveira, Thiago S. Moreira, Jan‐Marino Ramirezexpression studies. Plasmid constructs were acutely transfected into HEK293 cells using Viafect reagent (E4981; Promega, Madison, WI, USA), following the manufacturer’s protocol. In brief, HEK293 cells were prepared

(17) GATOR1 complex controls cisplatin sensitivityCell Death & DiseaseDecember 30, 2025Zhenrui Pan, Hanxiao Zhang, Xia Xiao, Catherine Brenner, Svetlana Dokudovskayaplasmids encoding Flag-NPRL2, Flag-NPRL3, or Flag-DEPDC5 using ViaFect™ Transfection Reagent (Promega, E4981) according to the manufacturer’s instructions. For each well, 100 ng plasmid DNA was mixed with ViaFect

(18) A bifunctional optical reporter for tracking estrogen response dynamics in neuronsbioRxivDecember 14, 2025Alexandra L. Cara, Eartha Mae Guthman, Aarya Mishra, Alexanne L. Hall, Sae Yokoyama, Norma P. Sandoval, Ian Gregg, Krisha Aghi, Kiran K. Soma, Stephanie M. Correa, et al.receptor and reporter DNA were transfected in a 1:3 ratio using the ViaFect Transfection Reagent (Promega E4981, Madison, WI) per supplier’s recommendations. Following another 24 h, hormone (17β-estradiol (E2) or

(19) Genetic evidence for PARP1 trapping as a driver of PARP inhibitor efficacy in BRCA mutant cancer cellsNucleic Acids ResDecember 11, 2025Jonathan Ribeiro, Kjetil Hansen, Lotte van Beek, Christopher Stubbs, Joan Frigola, Sara Talbot, Sabrina Bentouati, James Hall, Paul W G Wijnhoven, Josh Armenia, et al.transfected with pPARP1 (WT or L777P) -IRES-H2B-GFP vectors using the ViaFect Transfection Reagent (Promega, #E4981) and following the manufacturer’s instructions. Twenty-four hours post-transfection, media was changed.

(20) Repurposing clinically safe drugs for DNA repair pathway choice in CRISPR genome editing and synthetic lethalityNat CommunDecember 10, 2025Dominik Macak, Philipp Kanis, Stephan RiesenbergMMEJ substrate and 20 ng pGL4.53 (MMEJ). Transfection was performed using ViaFect reagent (Promega, E4981) at a ratio of 4 μl reagent per 1 μg of total DNA. For each well, the DNA mixture and the ViaFect reagent

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(1)

6/17/2019Easy-to-Use Transfection Reagent with Good Results from Promega Wei Yang SitHighly recommended transfection reagent for those who intend to carry out transfection.