Corning® Cell Recovery Solution, 100 mL from Corning Life Sciences

Product Specs
ItemCorning® Cell Recovery Solution, 100 mL
CompanyCorning Life Sciences
Price $60.55Supplier Page View Company Product Page
Catalog Number354253
Quantity1 / Pk
TypeDissociation and Recovery Reagents
ApplicationsAir-Liquid Interface (ALI) Cultures

Specifications/Features

Size: 100 mL

Add to Compare List

Corning Life Sciences

836 North Street

Building 300 Suite 3401

Tewksbury, MA 01876

United States

Phone: (978) 442-2200
Phone: (800) 492-1110

Email: clscustserv@corning.com

Company Profile

Website: www.corning.com/lifesciences

(1) Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosisDisease Models & MechanismsJune 1, 2026Muhammad D. R. Rahmana, Christopher J. Hill, Bettina Wilm, Dharani K. Hapangamawhen dense. At passage 2-3, EEOs were removed from Matrigel using Cell Recovery Solution (Corning, 354253) for endometrial assembloid generation. ESC culture ESCs were cultured in DMEM/Ham's F-12 medium supplemented

(2) Patient-derived kidney organoids recapitulate ADPKD and facilitate the identification of Rho pathway inhibitors as candidate therapeuticsCell Rep MedApril 21, 2026Liqiang Guo, Yujia Fan, Huixian Sun, Haoran Du, Hui Xiong, Yilin Li, Xinyan Zhang, Yong Guan, Lingtong Ren, Xuan Wang, et al.medium,Gibco,Cat#31985070 Polybrene,Beyotime,Cat#C0351-1 B-27,Gibco,Cat#1704-044 N-acetyl-L-cysteine,Sigma-Aldrich,Cat#A9165 Primocin™,InvivoGen,Cat#ant-pm-1 A83-01,TOCRIC,Cat#2939 Y-27632,Selleck,Cat#S1049 CHIR99021,Sigma-Aldrich,Cat#SML1046 Cell Recovery Solution,Corning,Cat#354253 DPBS,Gibco,Cat#C14190500CP Fluoroshield™ with DAPI,Sigma-Aldrich,Cat#F6057 Puromycin,Thermo Fisher Scientific

(3) Resistance to neoadjuvant talazoparib in triple-negative breast cancer by BRN2-induced ATR/STAT3 pathways or SHLD2 subclone expansionProc Natl Acad Sci U S AApril 21, 2026Noor M. Abdulkareem, Yan Jiang, Yuan Qi, Xuan Liu, Xiaomei Zhang, Shirong Cai, Jiansu Shao, Sabrina Jeter-Jones, Amanda L. Rinkenbaugh, Chun-Chun Cheng, et al.recovered from the matrigel matrix by incubating them with cell recovery solution (Corning, Cat. No. 354253) on ice for approximately 30 min, with frequent pipetting. Lentiviral Transduction for BRN2 Overproduction

(4) Novel NK cell-like phenotype expressing CCR5 and its ligands elicits tumor-specific acquired immunity to carcinomatous peritonitis via host NK-derived IFN-γJ Immunother CancerApril 20, 2026Situo Zheng, Yui Harada, Yosuke Morodomi, Noriko Yasuda, Kenta Ishimoto, Yoshikazu YonemitsuMatrigel plugs were harvested. Infiltrating cells were recovered after cell recover solution (Corning, 354253, New York, USA) digestion for 30 min at 4°C, following by Dispase (Corning, 354235, New York, USA) digestion

(5) Biobank of genetically defined murine prostate cancer tumoroids uncovers oncogenic pathways and drug vulnerabilities driven by PTEN-lossCell Reports MethodsApril 20, 2026Jessica Kalla, Thomas Dillinger, Zlata Pavlovicova, Reema Jacob, Emine Atas, Katarina Mišura, Anil Baskan, Kristina Draganić, Andreas Tiefenbacher, Tanja Limberger, et al.sparsely into ECM domes and grown for three days. To dissolve the ECM, Cell Recovery Solution (Corning #354253) was added, and using a microscope and pipette single organoids were transferred to fresh ECM domes

(6) RBFOX1 Dysfunction Unlocks APOE4-Associated Microglial Genesis and Exacerbates Alzheimer's Pathology in Human Cerebral OrganoidsExploration (Beijing)April 2, 2026Bowen Zhang, Changjie Shi, Jiayi Zhao, Qiuhong Hua, Houchun Zhang, Hailin Gao, Yuanyuan Qian, Jiaxue Cha, Jing Li, Jiayao Chen, et al.of Human Embryonic Stem Cells (H9) H9 were cultured on plates coated with Matrigel (Corning, Cat# 354253) at 37°C and 5% CO2 in mTeSR1 (Stem Cell Technologies, Cat# 85850). The medium was changed daily, and

(7) Synthetic budding morphogenesis by optogenetic receptor tyrosine kinase signalingbioRxivApril 2, 2026Louis S. Prahl, Ronald Canlla, Aria Zheyuan Huang, Daniel S. Alber, Sandra L. Shefter, Sachin N. Davis, Samuel H. Grindel, Zikang Dennis Huang, Thomas R. Mumford, William Benman, et al.iUB-optoRET organoids were extracted from Matrigel droplets using 600 μl of chilled Cell Recovery Solution (#354253, Corning) on ice for 20-30 min and were triturated approximately every 5 min. Organoids from each group

(8) Human receptive endometrial assembloid for deciphering the implantation windoweLifeApril 1, 2026Yu Zhang, Rusong Zhao, Chaoyan Yang, Jinzhu Song, Peishu Liu, Yan Li, Boyang Liu, Tao Li, Changjian Yin, Minghui Lu, et al.culture was rinsed with DPBS, and then the assembloid was harvested with cell recovery solution (Corning 354253) after incubating on ice for less than 30 min. Following centrifugation at 400×g for 5 min, the assembloid

(9) Ginsenoside Rg3 promotes chemosensitivity in lung adenocarcinoma organoids via apoptotic pathwaysFront PharmacolApril 1, 2026Min Liu, Yanxia Li, Bing Han, Ni Zeng, Xiuhua Wei, Xiaoli Xing, Zhongmin Jiang, Xiaozhi Liu, Chunyan Zhangdisrupted by pipette tip trituration, followed by incubation with organoid recovery solution (Corning, 354253) on ice for 15 min. Released organoid spheroids were pelleted at 150 g for 3 min, resuspended in dissociation

(10) Gastric epithelium from BRCA1 and BRCA2 carriers harbors increased double-stranded DNA damage and enhanced growth potentialMolecular Cancer ResearchMarch 23, 2026Kole H. Buckley, Michaela E. Dungan, Kevin Dinh, Gregory M. Kelly, Ryan Hausler, Kate E. Bennett, Daniel G. Clay, Julia E. Youngman, Ariana D. Majer, Keely A. Beyries, et al.from six wells of the tissue culture plate and replaced with cold Cell Recovery Solution (Corning; 354253). The plate was then placed on a rocker for 35 minutes at 4°C. Next, the six Matrigel “domes” containing

(11) Exploring roles of neuroglobin in oxygen homeostasis and neurodevelopment based on human cerebral organoid modelsiScienceMarch 20, 2026Yuan-Hsuan Liu, Meng-Ting Chung, Hsi-Chieh Lin, Hsin-Lei Lin, Hsuan Lin, Ya-Jen Cheng, Hsiao-Mei Wu, Tzyy-Wen Chiou, Yi-Chung Tungand magnesium-free DPBS,Thermo Fisher Scientific,Cat# 14190144 Cell Recovery Solution,Corning,Cat# 354253 Neural Tissue Dissociation Kit,Miltenyi Biotec,Cat# 130-092-628 Calcein AM,Thermo Fisher Scientific,Cat#

(12) Microenvironmental acidosis drives PARP- and ATM inhibitor resistance in p53 deficient pancreatic canceriScienceMarch 20, 2026Renata Ialchina, Thomas René Simonsen, Henriette Berg Andersen, Arnaud Stigliani, Yifan Dai, Jiayi Yao, Tanja Larsen, Dominika Czaplinska, Luis A. Pardo, Albin Sandelin, et al.condition 4-6 confluent domes of organoids were resuspended in Corning Cell Recovery Solution (Cat#354253) to isolate cells from the Matrigel. Where indicated, organoids were treated with 0.22 % DMSO (Sigma,

(13) Type-I interferons drive the gastrointestinal inflammatory response in a mouse model of Parkinson’s diseaseGastro Hep AdvancesMarch 19, 2026Harrison Waters, Shuyan Chen, Elizabeth Vincan, Dustin J. Flanagan, Renate H.M. Schwab, Peter J. Crack, Juliet M. Taylor(Invitrogen, 15596018). Organoid samples were harvested using Corning Cell Recovery Solution (Corning, 354253) and pelleted before using the RNAeasy mini kit (Qiagen, 74104) as per the manufacturer’s instructions.

(14) Aerobic glycolysis drives differentiation of unilocular adipocytesJournal of Lipid ResearchMarch 17, 2026Alice Maestri, Min Cai, Ruby Schipper, Julia Backman, Alana Vannay, Anneli Olsson, Ewa Ehrenborg, Roland Nilsson, Carolina E. Hagberglocularity Adipocyte spheroids were collected on d30, incubated in pre-chilled cell recovery solution (Corning, #354253) at 4°C for 20 min to remove the Matrigel, fixed in 10% formalin for 30 min, and then stained with CellMask

(15) Optimized culture of primary human alveolar type II cell–derived 3D organoids from fibrotic lung tissue with phenotypic and metabolic profilingRespiratory ResearchMarch 7, 2026Lara-Jasmin Schröder, Julia Rückoldt, Stephanie Schubert, Lars Knudsen, Sabina-Marija Janciauskiene, Christopher Werlein, Mareike Knoll, Regina Engelhardt, Christina Petzold-Mügge, Jonas C. Schupp, et al.for flow cytometry labeling, we strongly recommend the utilization of Cell Recovery Solution (Corning, Cat. No.: 354253) before further processing. 300 µL Cell Recovery Solution were added to the domes to dissolve organoids

(16) Mutational Signatures and Clonal Hematopoiesis in Intestinal Metaplasia across Countries with Varying Stomach Cancer IncidenceCancer DiscoveryMarch 2, 2026Kie Kyon Huang, Takeshi Hagihara, Benedict Shi Xiang Lian, Zhi Xuan Ong, Shen Kiat Lim, Roxanne Hui Heng Chong, Supriya Srivastava, Jason Xing Kang, May Yin Lee, Angie Lay-Keng Tan, et al.Briefly, approximately 7,500 organoids were recovered from Matrigel using Cell Recovery Solution (cat. #354253, Corning) and embedded in iPGell (cat. #FNK-PG20-1, Genostaff) according to the manufacturer’s instructions.

(17) Ancestrally diverse autologous patient-derived organoid – immune cell co-culture platform for addressing immunotherapeutic outcome disparities in high-grade endometrial cancerCancer Research CommunicationsMarch 1, 2026Charlie Chung, Brian Yueh, Santhilal Subhash, Onur Eskiocak, Aaron Nizam, Marissa Viola, Pascal Belleau, Ariel Kredentser, Arisa Kapedani, Alexander Krasnitz, et al.growth kinetics. For splitting, PDOs were retrieved from Matrigel by Cell Recovery Solution (Corning, 354253) treatment for 30 minutes and then digested with TrypLE supplemented with Y-Factor for 10 to 20 minutes.

(18) Modulating the tumor immune phenotypes by radiotherapy: formulating and validating the combination therapy of radiation, PD-L1, and TIM-3 blockade in colorectal cancerJournal for ImmunoTherapy of CancerFebruary 24, 2026Xuan Xuan Wang, Chu Zhu, Yikan Sun, Xuyun Xie, Mengjia Wang, Yue Jin, Yanjun Gu, Liwen Qian, Liming Shi, Jiaqi Xu, et al.every 2–3 days and organoids were passaged every 7–10 days using Cell Recovery Solution (Corning, Cat# 354253) with mechanical dissociation and, when needed, TrypLE Express (Gibco, Cat# 12604013). PDOs were cryopreserved

(19) DNASE1L3 Mediates Hepatocellular Carcinoma Tumor Growth and Organoid Models via the Wnt/β-Catenin Signaling PathwayOncology ResearchFebruary 24, 2026Shulong Zhang, Yijun Zhao, Li Geng, Feihong Song, Li Feng, Jun Jiang, Qianqian Cai, Fei FanMatrigel and released the organoids by incubating for one hour at 4°C with a cell recovery solution (354253, Corning). The organoids were subsequently digested with TrypLE solution (12604021, Gibco) for 5–10

(20) MAPK-driven epithelial cell plasticity drives colorectal cancer therapeutic resistanceNatureFebruary 19, 2026Mark White, Megan L. Mills, Laura M. Millett, Kathryn Gilroy, Yourae Hong, Lucas B. Zeiger, Rosalin J. Simpson, Shania M. Corry, Amelia Ligeza, Tamsin R. M. Lannagan, et al.suspension as follows. Organoids were harvested 72 h after seeding and incubated in Cell Recovery Solution (354253, Corning) on ice for 20 min to remove Matrigel. Samples were centrifuged at 300g for 5 min at 4 °C,

Be the First to Write a Review!

How to Optimize 3D Spheroid Assays
October 29, 2025
Optimization starts with proper generation & handling of spheroids. read more