Fig 1: Efferocytosis of apoptotic cells regulates Dicer expression in macrophages during resolution of inflammation. In vitro cultured peritoneal macrophages (PM) from WT mice were co-incubated with apoptotic neutrophils (A), apoptotic thymocytes (B) or apoptotic Jurkat cells (C) for different times, expression of macrophage Dicer mRNA was measured by RT-qPCR (n = 6). (D) In vitro cultured BMDMs from WT mice were co-incubated with apoptotic thymocytes, Dicer mRNA expression was measured by RT-qPCR (n = 6). (E) Peritoneal macrophages from WT mice were co-cultured with PBS, apoptosis cell conditioned medium, or apoptotic thymocytes for 24 h. Dicer mRNA expression of peritoneal macrophages were measured by RT-qPCR (n = 6). (F) Peritoneal macrophages from WT mice were co-cultured with PBS, 1 ug/ml cytochalasin D (Abcam, ab143484, China), apoptotic thymocytes, or cytochalasin D together with apoptotic thymocytes for 24 h. Dicer mRNA expression of peritoneal macrophages were measured by RT-qPCR (n = 6). (G) WT mice were intraperitoneally injected PBS or apoptotic thymocytes. After 24 h, the mRNA expression of Dicer in peritoneal macrophages was measured by RT-qPCR (n = 6). (H) WT mice were intravenously injected with PBS or apoptotic thymocytes. Dicer mRNA expression in splenic cells were measured by RT-qPCR (n = 6) after 24 h. Error bars represent SEM. Statistical analysis was performed via unpaired two-tailed Student’s t test (A, C, G, H) or one-way ANOVA with Tukey’s post-hoc test for multiple comparisons (B, D, E, F). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001