DAPI (hydrochloride) from Cayman Chemical

Product Specs
ItemDAPI (hydrochloride)
CompanyCayman Chemical
Price $61.00Supplier Page View Company Product Page
Catalog Number14285
Storage-20
FormulaC16H15N5 • 2HCl
OriginCHN
Molecular Weight350.3
FormatA crystalline solid
Quantity5 mg

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Mapping Satellite Glial Cell Heterogeneity Reveals Distinct Spatial Organization and Implies Functional Diversity in the Dorsal Root GanglionAdvanced ScienceOctober 24, 2025Ole Andreas Ahlgreen, Mads Würgler Hansen, Jonas Baake, Thomas Engelbrecht Hybel, Rachele Rossi, Xin Lai, Ishwarya Sankaranarayanan, Johanne Amalie Pold, Lin Lin, Line Reinert, et al.(1:30 000; H3570, Invitrogen) in PBST and human tissue sections were incubated for 5 min with DAPI (1:5000, #14285, Cayman Chemical) in PBST. Coverslips were mounted using fluorescent mounting medium (Dako, S3023) for

(2) Molecular Consequences of CCN6 Variants Encoding WISP3 in Progressive Pseudorheumatoid DysplasiaInternational journal of molecular sciencesSeptember 11, 2025Guven Tasbicen G, Tufan A, Savsar B, Bulbul A, Tonbul Z, Guzel E, Ayhan DH, Timucin AC, Onat UI, Bayram Akcapinar G, et al.Rosemont, IL, USA) were used as secondary antibodies. Nuclei were counterstained with 300 nm DAPI (Cat. No. 14285, Cayman Chemical, Ann Arbor, MI, USA). Confocal images were acquired using a Zeiss LSM 700 microscope.

(3) Nageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathyNature communicationsMay 5, 2025Shiers SI, Mazhar K, Wangzhou A, Haberberger R, Lesnak JB, Ezeji NA, Sankaranarayanan I, Tavares-Ferreira D, Cervantes A, Funk G, et al.incubated in their respective secondary antibody (1:2000) with DAPI (1:5000; Cayman Chemical; Cat # 14285) diluted in blocking buffer for 1 h at room temperature. The sections were washed in 1X PBS and then

(4) Ubiquitin-mediated recruitment of the ATG9A-ATG2 lipid transfer complex drives clearance of phosphorylated p62 aggregatesMolecular Biology of the CellFebruary 1, 2025David G Broadbent, Colten M McEwan, Dasun Jayatunge, Emily G Kaminsky, Tsz-Min Tsang, Daniel M Poole, Bradley C Naylor, John C Price, Jens C Schmidt, Josh L AndersenScientific,BP337-100 SEA BLOCK Blocking Buffer,Thermo Fisher Scientific,37527 DAPI,Cayman Chemical Company,14285 Prolong Diamond Antifade mounting reagent,Thermo Fisher Scientific,P36961

(5) ephrin-B2 promotes nociceptive plasticity and hyperalgesic priming through EphB2-MNK-eIF4E signaling in both mice and humansPharmacological ResearchAugust 1, 2024Eric T. David, Muhammad Saad Yousuf, Hao-Ruei Mei, Ashita Jain, Sharada Krishnagiri, Hajira Elahi, Rupali Venkatesan, Kolluru D. Srikanth, Gregory Dussor, Matthew B. Dalva, et al.washed three times with 1X PBS for 5 minutes each. Secondary antibodies DAPI (1:5000, Cayman Chemical #14285), Alex Fluor 488 (1:2000, Invitrogen #A-11039) and Alex Fluor 555 (1:2000, ThermoFisher #A21428) were

(6) How to differentiate induced pluripotent stem cells into sensory neurons for disease modelling: a functional assessmentStem Cell Research & TherapyApril 5, 2024Anil Kumar Kalia, Corinna Rösseler, Rafael Granja-Vazquez, Ayesha Ahmad, Joseph J. Pancrazio, Anika Neureiter, Mei Zhang, Daniel Sauter, Irina Vetter, Asa Andersson, et al.slides were washed in 0.1 M phosphate buffer (PB, pH 7.4) and stained with DAPI (Cayman Chemical, cat# 14285). Slides were then washed twice in 0.1 M PB, air dried, and cover-slipped with Prolong Gold Antifade

(7) Genetics of cystogenesis in base-edited human organoids reveal therapeutic strategies for polycystic kidney diseaseCell Stem CellApril 4, 2024Courtney E. Vishy, Chardai Thomas, Thomas Vincent, Daniel K. Crawford, Matthew M. Goddeeris, Benjamin S. FreedmanPharmaceuticals,NB157 Biotinylated LTL,Vector Labs,FL-1320 DBA,Vector Labs,RL-1032 DAPI,Cayman Chemical,14285 Gentamicin,Gibco,15710-064 GTTR,AAT Bioquest,24300 Mefloquine,Milipore Sigma,M2319 Critical Commercial

(8) Highly specific σ2R/TMEM97 ligand FEM-1689 alleviates neuropathic pain and inhibits the integrated stress responseProc Natl Acad Sci U S ADecember 26, 2023Muhammad Saad Yousuf, James J. Sahn, Hongfen Yang, Eric T. David, Stephanie Shiers, Marisol Mancilla Moreno, Jonathan Iketem, Danielle M. Royer, Chelsea D. Garcia, Jennifer Zhang, et al.#T8578), peripherin (1:1000, EnCor Biotechnology #CPCA-Peri), and DAPI (1:10,000, Cayman Chemicals #14285). Antibodies were incubated at 4 °C overnight. The primary antibodies were washed with PBS-Tween 20

(9) Diroximel fumarate acts through Nrf2 to attenuate methylglyoxal-induced nociception in mice and decreases ISR activation in DRG neuronsbioRxiv : the preprint server for biologyDecember 23, 2023Yousuf MS, Moreno MM, Li J, He L, Royer D, Zhang J, Woodall BJ, Grace PM, Price TJ.antibodies for 1 hour at room temperature followed by three 1X PBS washes. DAPI (Cayman Chemicals, 14285) was dissolved in 1X PBS and washed twice with 1X PBS. Human DRGs were incubated with TrueBlack (Biotium

(10) The formation of ubiquitin rich condensates triggers recruitment of the ATG9A lipid transfer complex to initiate basal autophagybioRxivNovember 30, 2023David G Broadbent, Colten M McEwan, Tsz-Ming Tsang, Daniel M Poole, Bradley C Naylor, John C Price, Jens Christopher Schmidt, Joshua L AndersenScientific,BP337-100 SEA BLOCK Blocking Buffer,ThermoFisher Scientific,37527 DAPI,Cayman Chemical Company,14285 Prolong Diamond Antifade mounting reagent,Thermo Fisher Scientific,P36961

(11) How to differentiate induced pluripotent stem cells into sensory neurons for disease modelling: a comparison of two protocolsResearch SquareOctober 29, 2023Anil Kumar Kalia, Corinna Rösseler, Rafael Granja-Vazquez, Ayesha Ahmad, Joseph J. Pancrazio, Anika Neureiter, Mei Zhang, Daniel Sauter, Irina Vetter, Asa Andersson, et al.slides were washed in 0.1M phosphate buffer (PB, pH 7.4) and stained with DAPI (Cayman Chemical cat# 14285). Slides were then washed twice in 0.1M PB, air dried, and cover-slipped with Prolong Gold Antifade

(12) Fibroblast-derived PI16 sustains inflammatory pain via regulation of CD206(+) myeloid cellsBrain, behavior, and immunityAugust 1, 2023Garrity R, Arora N, Haque MA, Weis D, Trinh RT, Neerukonda SV, Kumari S, Cortez I, Ubogu EE, Mahalingam R, et al.Cat. 463011-C3). Following RNAscope, slides were incubated with DAPI (1:5000; Cayman Chemical; Cat # 14285) diluted in 0.1M PB at room temperature. Slides were then washed in 0.1M PB, air dried and coverslipped

(13) Mesenchymal Stem Cell Culture within Perfusion Bioreactors Incorporating 3D-Printed Scaffolds Enables Improved Extracellular Vesicle Yield with Preserved BioactivityAdvanced healthcare materialsAugust 1, 2023Kronstadt SM, Patel DB, Born LJ, Levy D, Lerman MJ, Mahadik B, McLoughlin ST, Fasuyi A, Fowlkes L, Van Heyningen LH, et al.(Invitrogen, A30107) coupled with a nuclear stain using a 1:10,000 dilution of DAPI (Cayman Chemical Company, 14285) for 30 min at room temperature. HUVECs were then washed three times in 1X PBS and imaged using an Olympus

(14) Generation of two induced pluripotent stem cell lines from catecholaminergic polymorphic ventricular tachycardia patients carrying RYR2 mutationsStem cell researchJune 1, 2023Kong X, Belbachir N, Zeng W, Yan CD, Navada S, Perez MV, Wu JC.(Table 2). Nuclei were stained with the DAPI (hydrochloride) reagent (Cayman Chemical Company, Cat #14285) for 5 min at room temperature. Images were then taken using the ECHO Revolve microscope. 3.5 Mycoplasma

(15) Single-nucleus RNA sequencing of pre-malignant liver reveals disease-associated hepatocyte state with HCC prognostic potentialCell GenomicsMay 10, 2023Rodrigo Carlessi, Elena Denisenko, Ebru Boslem, Julia Köhn-Gaone, Nathan Main, N. Dianah B. Abu Bakar, Gayatri D. Shirolkar, Matthew Jones, Aaron B. Beasley, Daniel Poppe, et al.Bovine Serum Albumin,Sigma-Aldrich,Cat# B6917 RNasin® Plus Ribonuclease Inhibitor,Promega,Cat# N2615 DAPI,Cayman Chemical,Cat# 14285 PI,Cayman Chemical,Cat# 14289 Accudrop Beads,BD Biosciences,Cat# 661612 "Target Retrieval Solution,

(16) Activation of α 6 -containing GABA A receptors induces antinociception under physiological and pathological conditionsPainMay 1, 2023Rodríguez-Palma EJ, De la Luz-Cuellar YE, Islas-Espinoza AM, Félix-Leyva AE, Shiers SI, García G, Torres-López JE, Delgado-Lezama R, Murbartián J, Price TJ, et al.in 0.1 M PB and incubated with respective secondary antibodies (1:2000) with DAPI (1:5000, Cat. No. 14285, Cayman Chemical, Ann Arbor, MI) diluted in blocking buffer for 2 h at room temperature. Human tissue

(17) Validation of σ 2 R/TMEM97 as a neuropathic pain target: Specificity, human expression and mechanism of actionbioRxivApril 18, 2023Muhammad Saad Yousuf, James J. Sahn, Eric T. David, Stephanie Shiers, Danielle M. Royer, Chelsea D. Garcia, Jennifer Zhang, Veronica M. Hong, Ayesha Ahmad, Benedict J. Kolber, et al.#T8578), peripherin (1:1000, EnCor Biotechnology #CPCA-Peri) and DAPI (1:10,000, Cayman Chemicals #14285). Antibodies were incubated at 4 degrees Celsius overnight. The primary antibodies were washed with

(18) Calcineurin associates with centrosomes and regulates cilia length maintenanceJournal of cell scienceApril 15, 2023Tsekitsidou E, Wong CJ, Ulengin-Talkish I, Barth AIM, Stearns T, Gingras AC, Wang JT, Cyert MS.min. The supernatant was removed, and the pellet was resuspended in 500 µl of DAPI (Cayman Chemical, 14285; 20 μg/ml solution in PBS). The cells were incubated with DAPI at room temperature for 15 min, protected

(19) Meteorin Alleviates Paclitaxel-Induced Peripheral Neuropathic Pain in MiceThe Journal of PainApril 1, 2023Ishwarya Sankaranarayanan, Diana Tavares- Ferreira, Lucy He, Moeno Kume, Juliet M. Mwirigi, Torsten M. Madsen, Kenneth A. Petersen, Gordon Munro, Theodore J. Price11307-2-AP,1:1000 Peripherin,EnCor Biotechnology Inc,Cat # CPCA- Peri,1:1000 DAPI,Cayman,"Item no, 14285",1:5000 Connexin 43,Cell Signaling Technologies,Cat # 3512S,1:1000 "IgG (H+L) Cross-Adsorbed Goat

(20) Inducible co-stimulatory molecule (ICOS) alleviates paclitaxel-induced neuropathic pain via an IL-10-mediated mechanism in female miceJournal of NeuroinflammationFebruary 11, 2023Ishwarya Sankaranarayanan, Diana Tavares-Ferreira, Juliet M. Mwirigi, Galo L. Mejia, Michael D. Burton, Theodore J. Priceantibody,Thermo Fisher,11307-2-AP,1:1000 Peripherin,EnCor Biotechnology Inc,CPCA- Peri,1:1000 DAPI,Cayman,14285,1:5000 GFAP,Neuro Mab,N206A/8-75-240,1:1000 "Goat anti Mouse IgG (H + L) Secondary Antibody, Alexa

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DAPI (hydrochloride) from Cayman Chemical

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Cayman Chemical

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DAPI (hydrochloride) from Cayman Chemical

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