Stop Solution for TMB Substrate from BioLegend

Product Specs
ItemStop Solution for TMB Substrate
CompanyBioLegend
Price $36.00Supplier Page View Company Product Page
Catalog Number423001
Quantity60 mL
ApplicationsELISA

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) All‐PEG‐Like Block Copolymers Self‐Assemble into Stealth Nanocarriers for Drug DeliveryAdvanced ScienceMarch 1, 2026Parul Sirohi, Brooke E. Silverstein, Yulia Shmidov, Sonal Deshpande, Joy Tong, Yvonne Y. Ma, Chinmay S. Potnis, Soumen Saha, Xinghai Li, Max R. Ney, et al.room temperature before stopping the reaction with the addition of 100 µL of Stop Solution (BioLegend #423001). The plates were read by quantifying absorbance at 450 nm on a Wallac 1420 Victor3 Plate Reader (Perkin

(2) Broadening SARS-CoV-2 Immunity by Combining ORFV and Protein-Based VaccinesVaccines (Basel)January 4, 2026Alena Reguzova, Melanie Müller, Madeleine Fandrich, Alex Dulovic, Ralf AmannBioLegend) and the reaction was stopped after sufficient color development by adding stop solution (Cat. No. 423001, BioLegend). All samples and controls were run in duplicate. Absorbance was measured at 450 nm, and

(3) Heterologous ORFV–Ad26 vaccination broadens antibody breadth and amplifies cellular immunity against SARS-CoV-2 spikeFrontiers in ImmunologyNovember 18, 2025Alena Reguzova, Verena Haug, Melanie Müller, Madeleine Fandrich, Alex Dulovic, Ralf AmannBioLegend) and the reaction was stopped after sufficient color development by adding stop solution (Cat. No. 423001, BioLegend). All samples and controls were run in duplicate. Absorbance was measured at 450 nm, and

(4) Heterologous prime-boost vaccination with VLA2001 and an ORFV-based vector enhances spike- and nucleocapsid-specific immunity in miceFrontiers in ImmunologySeptember 18, 2025Alena Reguzova, Verena Haug, Carina Metz, Melanie Müller, Madeleine Fandrich, Alex Dulovic, Ralf AmannBioLegend) and the reaction was stopped after sufficient color development by adding stop solution (cat. no. 423001, BioLegend). All samples and controls were run in duplicate. Absorbance was measured at 450 nm, and

(5) Mutant p53 exploits enhancers to elevate immunosuppressive chemokine expression and impair immune checkpoint inhibitors in pancreatic cancerImmunityJuly 8, 2025Dig B. Mahat, Heena Kumra, Sarah A. Castro, Emily Metcalf, Kim Nguyen, Ryo Morisue, William W. Ho, Ivy Chen, Brandon Sullivan, Leon H. Yim, et al.Finetek,Cat# 62550 Antigen Unmasking Solution,VECTOR Laboratories,Cat# H-3300 Stop Solution,BioLegend,Cat# 423001 Critical commercial assays,Critical commercial assays,Critical commercial assays FoxP3 Transcription

(6) Highly Effective mRNA-LNP Vaccine Against Respiratory Syncytial Virus (RSV) in Multiple ModelsVaccines (Basel)June 10, 2025Huarong Bai, Xueliang Yu, Yue Gao, Qin Li, Baigang Wen, Rongkuan Huwas added to each well and incubated in the dark for 10 min. Then, 50 µL of stop solution (BIOLEGEND, 423001) was added to each well. The absorbance values at 450 nm were measured using a multi-functional microplate

(7) Safety and efficacy of trifluridine/tipiracil +/- bevacizumab plus XB2001 (anti-IL-1α antibody): a single-center phase 1 trialSignal Transduct Target TherJanuary 17, 2025Marion Thibaudin, Nicolas Roussot, Chloé Burlot, Antonin Schmitt, Julie Vincent, Zoé Tharin, Leila Bengrine, Hélène Bellio, Aurélie Bertaut, Léa Hampe, et al.well and incubated at RT in the dark for 15 min. The reaction was stopped by adding stop solution (423001, BioLegend) to each well of the microplate and after gently vortexing the plate, the absorbance was

(8) GV1001, hTERT Peptide Fragment, Prevents Doxorubicin-Induced Endothelial-to-Mesenchymal Transition in Human Endothelial Cells and Atherosclerosis in MiceCellsJanuary 10, 2025Chen W, Kim S, Kim SY, Beheshtian C, Kim N, Shin KH, Kim RH, Kim S, Park NH.MA, USA), following the manufacturer’s protocols. The color reaction was stopped with Stop solution (#423001, BioLegend, San Diego, CA, USA), and absorbance was measured immediately at 450 nm using a plate reader

(9) An artificial transcription factor that activates potent interferon-γ expression in human Jurkat T CellsFront Mol MedJanuary 8, 2025Ashley King, Davis Noblitt, Olivia Sherron, Clara Kjerfve, Lydia Pless, Nicholas L. Truexadded to the wells (50 µL), incubated for 15 min, and quenched with 100 µL of stop solution (BioLegend, 423001). The absorbance of the wells was recorded at 450 nm, IFN-γ concentrations were calculated in Excel

(10) Suppressing Tymovirus replication in plants using a variant of ubiquitinPLoS pathogensJanuary 1, 2025De Silva A, Kim K, Weiland J, Hwang J, Chung J, Pereira HS, Patel TR, Teyra J, Patel A, Mira MM, et al.substrate A and B, BioLegend). The reaction was stopped by adding a BioLegend Stop Solution (Cat. No. 423001), turning the colour from blue to yellow. The absorbance of the plate was read at 450 nm using SpectraMax

(11) Heterologous prime-boost vaccination with VLA2001 and an ORFV-based vector enhances spike- and nucleocapsid-specific immunity in miceFrontiers in immunologyJanuary 1, 2025Reguzova A, Haug V, Metz C, Müller M, Fandrich M, Dulovic A, Amann R.BioLegend) and the reaction was stopped after sufficient color development by adding stop solution (cat. no. 423001, BioLegend). All samples and controls were run in duplicate. Absorbance was measured at 450 nm, and

(12) Isolation and Characterization of Antibodies Against Vascular Cell Adhesion Molecule-1 Reveals Putative Role for Ig-like Domains 2 and 3 in Cell-to-Cell InteractionInternational Journal of Molecular SciencesDecember 20, 2024Binura Perera, Yuao Wu, Jessica R. Pickett, Nadya Panagides, Francisca M. Barretto, Christian Fercher, David P. Sester, Martina L. Jones, Hang T. Ta, Lucia F. Zacchitemperature. After the final wash, plates were developed using TMB substrate (BioLegend, San Diego, CA, USA, 423001) following the manufacturer’s instructions. Absorbance at 450 nm was measured using a microplate spectrometer

(13) Engineered IgM and IgG cleaving enzymes for mitigating antibody neutralization and complement activation in AAV gene transferMolecular therapy : the journal of the American Society of Gene TherapyJuly 3, 2024Smith TJ, Elmore ZC, Fusco RM, Hull JA, Rosales A, Martinez M, Tarantal AF, Asokan A.were washed and incubated with 3,3′5,5′-tetramethylbenzidine high sensitivity substrate (BioLegend, 423001) for 20 min before adding stop solution (BioLegend, 423001). Absorbance was measured at 450 nm using

(14) Evaluation of urinary C-reactive protein as an early detection biomarker for pancreatic ductal adenocarcinomaFrontiers in oncologyJanuary 1, 2024Ali N, Debernardi S, Kurotova E, Tajbakhsh J, Gupta NK, Pandol SJ, Wilson P, Pereira SP, Greenhalf B, Blyuss O, et al.urines were diluted 1:750. The substrate (TMB) and stop solution were from BioLegend (Cat# 421101 and 423001). The absorbance was determined with a microplate reader (FLUOstar® Omega, BMG LABTECH, Offenburg, Germany)

(15) An Animal Model for Chronic Meningeal Inflammation and Inflammatory Demyelination of the Cerebral CortexInternational journal of molecular sciencesSeptember 9, 2023Enz LS, Winkler A, Wrzos C, Dasen B, Nessler S, Stadelmann C, Schaeren-Wiemers N.solution (421101, BioLegend) was added per well. Upon sufficient color development, 100 μL stop solution (423001, BioLegend) was added. Absorption was read at 450 nm. 4.6. Animals Adult female inbred Lewis rats

(16) Mapping the T cell repertoire to a complex gut bacterial communityNatureSeptember 1, 2023Nagashima K, Zhao A, Atabakhsh K, Bae M, Blum JE, Weakley A, Jain S, Meng X, Cheng AG, Wang M, et al.evaluated by measuring IL-2 concentration in culture supernatant using an IL2 ELISA kit (catalogue nos. 423001, 423501, 431001 and 421101, BioLegend). Antigen discovery using E. coli shotgun genomic libraries E. coli

(17) Crosstalk between ILC2s and Th2 cells varies among mouse modelsCell reportsFebruary 28, 2023Gurram RK, Wei D, Yu Q, Butcher MJ, Chen X, Cui K, Hu G, Zheng M, Zhu X, Oh J, et al.with the TMB substrate solution (Cat# 421101; Biolegend). The stop solution for TMB substrate (Cat# 423001; Biolegend) was used to terminate the reaction. Final absorbance was measured at 450 nm. The concentration

(18) Primary and secondary functions of HLA-E are determined by stability and conformation of the peptide-bound complexesCell ReportsJune 14, 2022Lucy C. Walters, Daniel Rozbesky, Karl Harlos, Max Quastel, Hong Sun, Sebastian Springer, Robert P. Rambo, Fiyaz Mohammed, E. Yvonne Jones, Andrew J. McMichael, et al.(x20),Biolegend,Cat#421501 TMB High sensitivity Substrate,Biolegend,Cat#421501 Stop Solution,Biolegend,Cat#423001 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, NativePAGE™ Sample Buffer (4X

(19) Inflammation and neutrophil extracellular traps in cerebral cavernous malformationCellular and molecular life sciences : CMLSMarch 25, 2022Yau ACY, Globisch MA, Onyeogaziri FC, Conze LL, Smith R, Jauhiainen S, Corada M, Orsenigo F, Huang H, Herre M, et al.then incubated with 3,3',5,5'-tetramethylbenzidine (T0440; Sigma-Aldrich) in the dark. Stop solution (423001; Biolegend) was added to terminate the signal development. The absorbance was determined at 450 nm,

(20) A Low Dose Combination of Withaferin A and Caffeic Acid Phenethyl Ester Possesses Anti-Metastatic Potential In Vitro: Molecular Targets and MechanismsCancersFebruary 3, 2022Sari AN, Dhanjal JK, Elwakeel A, Kumar V, Meidinna HN, Zhang H, Ishida Y, Terao K, Sundar D, Kaul SC, et al.(BioLegend Inc., BioLegend Way, San Diego, CA, USA) for 30 min, followed by the addition of 100 µL Stop Solution (423001) (BioLegend Inc., BioLegend Way, San Diego, CA, USA). Optical density was measured at 450 nm using an

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Stop Solution for TMB Substrate from BioLegend

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Stop Solution for TMB Substrate from BioLegend

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