OptiPrep - Density Gradient Media (Iodixanol) from Cosmo Bio USA

Product Specs
ItemOptiPrep - Density Gradient Media (Iodixanol)
CompanyCosmo Bio USA
Price $181.00Supplier Page View Company Product Page
Catalog NumberAXS-1114542
Quantity1x250ml, 5x250ml, 10x250ml

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Cosmo Bio USA

2792 Loker Avenue West, Suite 101

Carlsbad, California 92010

United States

Phone: Phone/Fax: +1 (760) 431-4600

Company Profile

Website: cosmobiousa.com

(1) Treatment of age-related decreases in GTP levels restores endocytosis and autophagyGeroScienceAugust 2, 2025R. A. Santana, J. M. McWhirt, G. J. Brewerthe top of a 15 mL tube prepared with four layered densities of Optiprep (Cosmo Bio, Carlsbad, CA, AXS-1114542). The gradient was centrifuged at 800 g for 15 min. The neuron-enriched fractions were collected and

(2) Metabolic engineering improves transduction efficiency and downstream vector isolation by altering the lipid composition of extracellular vesicle-enclosed AAVMetabolic EngineeringMarch 1, 2025Paula Espinoza, Ming Cheng, Carrie Ng, Demitri de la de la Cruz, Elizabeth D. Wasson, Deirdre M. McCarthy, Pradeep G. Bhide, Casey A. Maguire, Miguel C. Santoscoyprepared 40%, 25%, and 10% v/v iodixanol solutions dissolved in PBS using the 60%w/v Iodixanol OptiPrep™ (AXS-1114542 Cosmo Bio USA) as previously reported(Cheng et al., 2021). We prepared gradients with increased density

(3) Optimization of ultracentrifugation-based method to enhance the purity and proteomic profiling depth of plasma-derived extracellular vesicles and particlesJ Extracell BiolJuly 1, 2024Wan Z, Gu J, Balaji U, Bojmar L, Molina H, Heissel S, Pagano AE, Peralta C, Shaashua L, Ismailgeci D, et al.(w/v), 20% (w/v), 10% (w/v), 5% (w/v) solutions of iodixanol were made by diluting OptiPrep (Cosmo Bio, AXS‐1114542) (60% (w/v)) with sucrose solution (0.25 M, 10 mM Tris, pH 7.5). To set the gradient, 3 mL of 40% iodixanol

(4) Skull progenitor cell-driven meningeal lymphatic restoration improves neurocognitive functions in craniosynostosisCell stem cellNovember 2, 2023Ma L, Chang Q, Pei F, Liu M, Zhang W, Hong YK, Chai Y, Chen JF.extracted from cell lysates using iodixanol step gradients (60% (wt/vol) iodixanol; Cosmo Bio USA, Cat #AXS-1114542–5) and ultracentrifugation at speed of 350000g at 18°C. AAVs were then filtered by Syringe filter units

(5) Spatial atlas of the mouse central nervous system at molecular resolutionNatureOctober 19, 2023Hailing Shi, Yichun He, Yiming Zhou, Jiahao Huang, Kamal Maher, Brandon Wang, Zefang Tang, Shuchen Luo, Peng Tan, Morgan Wu, et al.were centrifuged at 2,000g, and the supernatant was loaded over iodixanol (Optiprep, Cosmo Bio USA, AXS-1114542) step gradients (15%, 25%, 40%, and 60%). Viruses were extracted from the 40/60% interface and the 40%

(6) Structural basis for the allosteric modulation of rhodopsin by nanobody binding to its extracellular domainNat CommunAugust 25, 2023Arum Wu, David Salom, John D. Hong, Aleksander Tworak, Kohei Watanabe, Els Pardon, Jan Steyaert, Hideki Kandori, Kota Katayama, Philip D. Kiser, et al.0.02 mM EDTA, pH 7.4). Isolated retinas were suspended in 0.08 ml of 8% (v/v) OptiPrep (catalog no. AXS1114542, Cosmo Bio USA, Inc, CA, USA) in Ringer’s buffer and vortexed at maximum speed for 1 min. The samples

(7) Single-swap editing for the correction of common Duchenne muscular dystrophy mutationsMolecular therapy. Nucleic acidsJune 13, 2023Chai AC, Chemello F, Li H, Nishiyama T, Chen K, Zhang Y, Sánchez-Ortiz E, Alomar A, Xu L, Liu N, et al.Children’s Hospital Viral Core. AAV vectors were purified by discontinuous iodixanol gradients (Cosmo Bio; AXS-1114542-5) and concentrated with a Millipore Amicon filter unit (UFC910008, 100 kDa). AAV titers were determined

(8) Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectorsScience advancesApril 21, 2023Shay TF, Sullivan EE, Ding X, Chen X, Ravindra Kumar S, Goertsen D, Brown D, Crosby A, Vielmetter J, Borsos M, et al.ArcticZymes, 70910-202). The resulting lysate was extracted from an iodixanol (Cosmo Bio USA, OptiPrep, AXS-1114542) step gradient following ultracentrifugation. Purified virus was concentrated and buffer-exchanged with

(9) Robust regulatory architecture of pan-neuronal gene expressionCurrent BiologyApril 1, 2022Eduardo Leyva-Díaz, Oliver HobertAzide,Sigma-Aldrich,Cat# 71289 RNase-free DMF,Acros Organics,Cat# AC327175000 OptiPrep,Cosmo Bio USA,Cat # AXS-1114542 Dynabeads Protein G,Thermo Fisher,Cat# 10003D Dynabeads M-270 Carboxylic Acid,Thermo Fisher,Cat# 14305D Alt-R S.p. Cas9 Nuclease V3

(10) Exploring rare cellular activity in more than one million cells by a transscale scopeScientific ReportsDecember 1, 2021T. Ichimura, T. Kakizuka, K. Horikawa, K. Seiriki, A. Kasai, H. Hashimoto, K. Fujita, T. M. Watanabe, T. Nagaifor 15 min at room temperature. Viral vectors were purified using iodixanol (Optiprep, catalogue no. AXS-1114542; Cosmo Bio Co., Tokyo, Japan) density gradient ultracentrifugation. The viral titers were determined

(11) Specific and behaviorally consequential astrocyte G_q GPCR signaling attenuation in vivo with iβARKNeuronJuly 21, 2021Nagai J, Bellafard A, Qu Z, Yu X, Ollivier M, Gangwani MR, Diaz-Castro B, Coppola G, Schumacher SM, Golshani P, et al.were clarified by centrifugation at 2,000g and then purified over iodixanol (Optiprep, Cosmo Bio USA, AXS-1114542–5) density gradients (15%, 25%, 40% and 60%). Viruses were concentrated using Amicon filters (Millipore,

(12) BB-301: a silence and replace AAV-based vector for the treatment of oculopharyngeal muscular dystrophyMolecular therapy. Nucleic acidsJune 4, 2021Strings-Ufombah V, Malerba A, Kao SC, Harbaran S, Roth F, Cappellari O, Lu-Nguyen N, Takahashi K, Mukadam S, Kilfoil G, et al.an iodixanol gradient of layered 60%, 40%, 25%, and 15% iodixanol (OptiPrep, Cosmo Bio, catalog no. AXS-1114542) and centrifuged at 18°C at 350,000 × g for 1 h. The AAV-enriched, 40% iodixanol layer was polished

(13) Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editingScience advancesApril 1, 2021Chemello F, Chai AC, Li H, Rodriguez-Caycedo C, Sanchez-Ortiz E, Atmanli A, Mireault AA, Liu N, Bassel-Duby R, Olson EN.previously described (58). AAV vectors were purified by discontinuous iodixanol gradients (Cosmo Bio, AXS-1114542-5) and concentrated with a Millipore Amicon filter unit (UFC910008, 100 kDa). AAV titers were determined

(14) Age-Related Intraneuronal Aggregation of Amyloid-β in Endosomes, Mitochondria, Autophagosomes, and LysosomesJournal of Alzheimer's DiseaseJanuary 7, 2020Gregory J. Brewer, Robert A. Herrera, Stephan Philipp, Justyna Sosna, Jorge Mauricio Reyes-Ruiz, Charles G. Glabehippocampus and hemicortex was transferred to a separate 15 mL tube of Optiprep (Cosmo Bio, Carlsbad, CA, AXS-1114542) of 4 layered densities. The two gradients were centrifuged at 800 g for 15 min. The neuron enriched

(15) Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategiesNature communicationsApril 26, 2019Rajendran PS, Challis RC, Fowlkes CC, Hanna P, Tompkins JD, Jordan MC, Hiyari S, Gabris-Weber BA, Greenbaum A, Chan KY, et al.(ArcticZymes, 70910-202). Clarified lysates were purified over iodixanol (Cosmo Bio USA, OptiPrep, AXS-1114542) step gradients (15, 25, 40, and 60%)69. Purified viruses were concentrated, washed in sterile phosphate

(16) CRISPR-Cas9 corrects Duchenne muscular dystrophy exon 44 deletion mutations in mice and human cellsScience advancesMarch 1, 2019Min YL, Li H, Rodriguez-Caycedo C, Mireault AA, Huang J, Shelton JM, McAnally JR, Amoasii L, Mammen PPA, Bassel-Duby R, et al.previously described (39). AAV vectors were purified by discontinuous iodixanol gradients (Cosmo Bio, AXS-1114542-5) and then concentrated with Millipore Amicon filter unit (UFC910008, 100 kDa). AAV titers were determined

(17) Hierarchical and stage-specific regulation of murine cardiomyocyte maturation by serum response factorNature CommunicationsDecember 1, 2018Yuxuan Guo et al.lysis buffer, and pooled with cell lysates. AAV was purified in a density gradient (Cosmo Bio USA, AXS-1114542) by ultracentrifugation (Beckman, XL-90) with a VTi-50 rotor and concentrated in phosphate-buffered

(18) Cell-free Fluorescent Intra-Golgi Retrograde Vesicle Trafficking AssayBIO-PROTOCOLNovember 20, 2017Nathanael P. Cottam, Daniel Ungar2017 DOI:10.21769/BioProtoc.2616 20. Optiprep density gradient media (Axis-Shield PoC) (Cosmo Bio, catalog number: AXS-1114542) 21. α-HA antibody (monoclonal anti-HA.11) (BioLegend, catalog number: 901513) 22. Dithiothreitol (DTT) 23.

(19) Cell-free Fluorescent Intra-Golgi Retrograde Vesicle Trafficking AssayBio ProtocNovember 20, 2017Nathanael P. Cottam, Daniel UngarN4888)Liquid nitrogenTrisOptiprep density gradient media (Axis-Shield PoC) (Cosmo Bio, catalog number: AXS-1114542)α-HA antibody (monoclonal anti-HA.11) (BioLegend, catalog number: 901513)Dithiothreitol (DTT)Golgi membranes,

(20) Absence of Either Ripk3 or Mlkl Reduces Incidence of Hepatocellular Carcinoma Independent of Liver FibrosisMolecular cancer research : MCRJanuary 1, 0001"Mohammed S, Thadathil N, Ohene-Marfo P, Tran AL, Van Der Veldt M, Georgescu C, Oh S, Nicklas EH, Wang D, Haritha NH, et al."and pelleted NPC was subjected to differential centrifugation using OptiPrep density gradient media (#AXS-1114542, Cosmo Bio USA) at 1,500 × g for 30 minutes. The interphase containing NPC was collected and pelleted

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OptiPrep - Density Gradient Media (Iodixanol) from Cosmo Bio USA

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OptiPrep - Density Gradient Media (Iodixanol) from Cosmo Bio USA

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