DAPI from Abcam

Product Specs
ItemDAPI
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab285390
Quantity5 mg, 25 mg
TypeChemical
Molecule NameDAPI

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Abcam

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(1) hUC-MSCs via β-NGF Alleviate Cognitive Impairment After Tibial Fracture Surgery by Regulating the STMN2/NMNAT2-SARM1-NF-κB Signaling PathwayCNS Neurosci TherJuly 29, 2026Kai Wang, Fangjun Wang, Yuhan Zhang, Wenbo Li, Shuai Li, Ying Zhou, Guangkuo Ma, Ziwei Xia, Xueyan Zhou, Liwei Wangat room temperature. Slices were mounted using Antifade Mounting Medium containing DAPI (Abcam, UK, ab285390) and visualized with a laser scanning confocal microscope (Olympus, Japan, Olympus FV1000). 2.14 Golgi‐Cox

(2) Mst1 participates in the progression of severe acute pancreatitis-induced intestinal barrier dysfunction via inhibiting autophagy and enhancing apoptosisSAGE Open MedJuly 13, 2026Rong Zhang, Ziwen Liu, Tao Lin, Rui Shen, Chuqi Xiang, Pengtao Zhao, Wang LuTUNEL assay kit following the manufacturer’s instructions. Cell nuclei were counterstained with DAPI (#ab285390, Abcam). TUNEL-positive cells were imaged using a fluorescence microscope (Nikon Eclipse NI-E; Nikon

(3) Exertional heat stroke-related acute kidney injury: novel animal and cellular modelsRen FailJune 10, 2026Jiaona Liu, Jing Hao, Pengfei Kang, Yingjie Zhang, Wanjun Shen, Pu Chen, Jingjing Chen, Xuefeng Sun, Xiangmei Chenthe dark. The cells were then washed three times with PBS and counterstained with DAPI (50 μl, Abcam, ab285390) at room temperature for 10 min. Finally, images were observed and captured using a Bio-Rad ZOE fluorescence microscope

(4) Multi-omics profiling identifies inosine as a key metabolite associated with embryonic arrest in hydrosalpinx-associated infertilityEBioMedicineMay 21, 2026Yuyang Peng, Yiwei Fang, Nan Wang, Xiaomeng Wang, Dandan Liu, Ming Li, Yan Liu, Liying Yan, Peng Yuan, Huamao Liang, et al.conjugated to Alexa Fluor 594 or 488 (Invitrogen; 1:200) and 4’,6-diamidino-2-phenylindole (DAPI) (1:200, ab285390, Abcam) for 1 h at room temperature, followed by three washes with PBS. Images were captured with a

(5) Targeting ANGPTL3 and IL‐33/ST2 Ameliorates Diabetic Kidney Disease by Reducing Lipotoxicity, Alleviating Inflammation and Inhibiting FibrosisAdvanced ScienceMay 19, 2026Zhuojin Li, Zhonglian Cao, Rongrui Zhou, Xu Cheng, Xiaozhi Hu, Shuwen Xu, Zihan Dou, Ling Du, Dianwen JuEPR23094‐120, 1:200 dilution), all sourced from Abcam (Cambridge, UK). Nuclei were counterstained with DAPI (ab285390, Abcam, 1:1000 dilution). Positively stained areas were quantified from SlideView VS200 images using

(6) The dynamic expression pattern of signaling pathways involved in early embryo developmentCell Communication and SignalingMay 7, 2026Zifan Song, Shi Song, Nan Wang, Xiaomeng Wang, Liying Yan, Jie Qiao, Peng YuanFluor-conjugated secondary antibodies at 37 °C for 2 h. Nuclei were stained with 1 µg/mL DAPI (Abcam, ab285390). All antibodies used in this study were diluted in 5% BSA. The dilution ratios were as follows: mouse

(7) Clinical Significance for risk stratification of papillary thyroid cancer by TUMORFISHER circulating tumor cells techniqueClinical Cancer ResearchApril 1, 2026Liehao Jiang, Xuejie Li, Tianyi Ma, Pingping Zeng, Zhuo Tan, Chuanming Zheng, Jiajie Xu, Lizhuo Zhang, Yaweng Guo, Juyong Liang, et al.(BioLegend, #324203) or cytokeratin (CK) mix (CKmix; Abcam, #ab26448), followed by DAPI staining (Abcam, #ab285390). Flow cytometry (FCM; BD Accuri C6 and FlowJo) and fluorescence microscopy (Olympus IX73 and ImageJ)

(8) Protease-mediated PRC1 dissociation promotes H2AK119ub remodeling during stress responsesThe EMBO JournalMarch 11, 2026Wei Cui, Qingyang Li, Jinsong Wei, Ting Zhou, Haozhe Zhu, Delai Huang, Shuai Wang, Jianan Gao, Ru Zhou, Zeyu Sun, et al.chloride, anhydrous",Sangon Biotech,Cat# 233-140-8 Glycerol,Sangon Biotech,Cat# 231-791-2 DAPI,Abcam,Cat# ab285390 Alexa Fluor-488,Abcam,Cat# ab150077 Alexa Fluor-647,Abcam,Cat# ab150115 β-Mercaptoethanol,Febio science,Cat#

(9) The TRAF6/SPP1 axis participates in osteoarthritis progression through regulating the catabolism and anabolism of cartilage matrixScientific ReportsMarch 4, 2026Jiapei Yao, Jiliu Huang, Chongrui Li, JingJing Shang, Xiaolong Lin, Xindie Zhou, Yaojun Lu(PA5-95599, Invitrogen, USA), β-actin (ab8226, Abcam, UK), and 4′−6-diamidino-2-phenylindole (DAPI) (ab285390, Abcam, UK), all sourced from Abcam. Additional reagents included MMP13 (18165-1-AP, Proteintech, USA),

(10) Modulation of Macrophage ferroptosis under the guide of infrared thermography promotes the healing of pressure injuriesJournal of Advanced ResearchFebruary 1, 2026Xiaoqiong Jiang, Xuanlong Zhang, Huiming Deng, Lulu Lin, Yu Wang, Yuqi Wang, Jiayi Huang, Ningning Yang, Shi Xu, Jian Wang, et al.sections were treated with secondary antibodies for 1 h at 37 °C and then stained with DAPI (Abcam, ab285390) solution. Positive cells were captured using a fluorescence microscope. The expression levels of iNOS,

(11) Rochelle salt-based biodegradable piezoelectric devices for nerve regeneration and intestinal motility monitoringNature CommunicationsJanuary 29, 2026Fanqi Dai, Haofeng Cheng, Hui Qi, Honglai Cai, Abdul Aziz, Tianqi Su, Yanjun Guan, Yuhui Cui, Jingwei Meng, Handi Deng, et al.ab150117, Abcam), was incubated at room temperature for 2 h. Nuclei were stained with DAPI (1:5000, ab285390, Abcam) for 15 min. Images were acquired using a confocal panoramic scanner (3DHISTECH, Panoramic Confocal,

(12) Ginkgolide C alleviates atherosclerosis via activating Nrf2 to inhibit ROS‑dependent NLRP3 inflammasome activationInternational Journal of Molecular MedicineJanuary 27, 2026Rui Zhangpurchased from Abcam. Nuclear visualization employed DAPI staining (0.1 μg/ml PBS; 5 min; cat. no. ab285390; Abcam). Confocal microscopy (Leica TCS SP8, 40x oil lens, NA 1.30) captured images using LAS X 3.7.4

(13) AAV-DJ–Mediated MYOC Silencing as a Gene Therapy Approach for Myocilin-Associated GlaucomaInvestigative Ophthalmology & Visual ScienceJanuary 22, 2026Yue Wan, Xiangxiang Liu, Xuejing Yan, Shen Wu, Yufei Teng, Luyi Han, Jingxue Zhang, Ningli Wang(1:1000, ab150077; Abcam). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1:10,000, ab285390; Abcam). Confocal images were acquired using a Leica DMI6000 B inverted microscope (Leica Microsystems,

(14) Berberine Alleviates Shigella-Induced Dysentery by Regulating Intestinal Barrier and Inflammatory ResponsesInternational Journal of Molecular SciencesJanuary 21, 2026Jinwen Ding, Yu Zhang, Xinyu Fan, Yanxing Han, Yuan Lin(1:1000, CST91131) primary antibodies overnight at 4 °C. And then the nucleus was stained with DAPI (ab285390, Abcam, Cambridge, MA, USA). Images were collected on Leica TCS SPS microscope (Wetzlar, Germany). 4.7.

(15) Clinical Significance for Risk Stratification of Papillary Thyroid Cancer by TUMORFISHER Circulating Tumor Cell TechniqueClin Cancer ResJanuary 16, 2026Liehao Jiang, Xuejie Li, Tianyi Ma, Pingping Zeng, Zhuo Tan, Chuanming Zheng, Jiajie Xu, Lizhuo Zhang, Yaweng Guo, Juyong Liang, et al.(BioLegend, #324203) or cytokeratin (CK) mix (CKmix; Abcam, #ab26448), followed by DAPI staining (Abcam, #ab285390). Flow cytometry (FCM; BD Accuri C6 and FlowJo) and fluorescence microscopy (Olympus IX73 and ImageJ)

(16) Regulation of Periodontal Ligament Autophagy by Super-Activated Platelet Lysate in a Rat Maxillary Expansion ModelDrug Des Devel TherJanuary 14, 2026Yang Liu, Jianan Li, Huiying Lu, Xiaoyao Liu, Yang Li, Yi Zhang, Peijun Wang(ab192890, Abcam), along with corresponding secondary antibodies (ab150116/ab150073, Abcam) and DAPI (ab285390, Abcam) for nuclear counterstaining. All images were observed and captured using a Leica DM3000 LED

(17) Regulation of Periodontal Ligament Autophagy by Super-Activated Platelet Lysate in a Rat Maxillary Expansion ModelDrug Design, Development and TherapyJanuary 14, 2026Yang Liu, Jianan Li, Huiying Lu, Xiaoyao Liu, Yang Li, Yi Zhang, Peijun Wang(ab192890, Abcam), along with corresponding secondary antibodies (ab150116/ab150073, Abcam) and DAPI (ab285390, Abcam) for nuclear counterstaining. All images were observed and captured using a Leica DM3000 LED

(18) PCID2 is essential for spermatogonial differentiation by regulating alternative splicingCellular and Molecular Life SciencesJanuary 13, 2026Feiyin Zhu, Ying Zhang, Yu Xi, Chenjia Gong, Yanlin Tang, Yidong Chen, Liying Yan, Jie Qiao, Qiang Liuslices were incubated with secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI, 1:1000, Abcam, ab285390) to stain the nuclei at room temperature for 1 h. The cells were then washed three times with PBST.

(19) YAP1 inhibition protects retinal vascular endothelial cells under high glucose by inhibiting autophagyOpen Life SciDecember 24, 2025Yu-Shan Li, Wen-Qiang Liu, Hong-Dan Yu, Zhong-Fu Zuo, Xue-Zheng Liudilution at 37°C. Post-incubation, the slides were treated with 4′,6-diamidino-2-phenylindole (DAPI) (ab285390) for 5 min in the dark, and subsequently, images were captured using a fluorescence microscope (IX81,

(20) The Neuroprotective Effect of 4-Octyl Itaconate on Acute Period of Experimental Autoimmune NeuritisInflammationDecember 17, 2025Lin-Jie Zhang, Ning Zhao, Jia Li, Hui Zhai, Jie Wu, Li YangiNOS, 1:200, Abcam, USA, cat#ab178945), fluorescent secondary antibodies, and DAPI (Abcam, USA, cat#ab285390), then observed by fluorescence microscopy (Olympus, Japan). MDA, SOD, ROS Detection MDA, SOD and

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