Fig 1: Methyltransferase‐like 3 (METTL3) promotes cervical cancer cell migration and invasion in vitro. (A) Western blot analysis of cervical cancer cells transfected with normal control (siNC), METTL3‐targeted (siMETTL3#1, siMETTL3#2) siRNAs, and empty vector (EV), or METTL3‐overexpression (METTL3) vector. (B) RT‐quantitative PCR analysis of knockdown and overexpression efficiency. (C–F) Images and quantification of Transwell migration assays of METTL3‐knockdown and ‐overexpressing cells. Scale bar, 100 μm. (G–J) Images and quantification of wound healing assays of METTL3‐knockdown and ‐overexpressing cells. The average spacing of scratches at 0 and 36 h and 0 and 24 h was compared. Scale bar, 100 μm. (K–N) Images and quantification of Transwell invasion assays of METTL3‐knockdown and ‐overexpressing cells. Scale bar, 100 μm. (O–R) Images and quantification of migration (right) and invasion (left) assays of CaSki and SiHa cells treated with an METTL3 inhibitor (METTL3i) for 48 h (10 μM, STM2457, HY‐134836, MCE). Scale bar, 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001