Fig 1: Combined PXN silencing and TMZ treatment enhances apoptosis in GBM cells.A qRT-PCR detecting PXN mRNA expression in U87 cells pre-treated with DMSO or TMZ (MCE, HY-17364). Data are presented as mean ± SD of three independent biological replicates. Statistical significance was determined by a two-tailed Student’s t-test (***P < 0.001). B Western blotting determining PXN protein expression in U87 cells pre-treated with DMSO or TMZ. C Cell viability assay detecting proliferation ability of U87 and XJ6376 cells subjected to the indicated treatments. Data are presented as mean ± SD of three independent biological replicates. Statistical significance was determined by a one-way ANOVA with Tukey’s post hoc test (***P < 0.001). D Colony formation assay detecting tumorigenic potential of U87 cells subjected to the indicated treatments. Scale bar = 500 μm. E Western blotting determining cleaved caspase-3 protein expression in U87 cells subjected to the indicated treatments. F In situ fluorescence showing caspase-3 activity in living XJ6376 cells. Data are presented as mean ± SD of three independent biological replicates. Statistical significance was determined by a one-way ANOVA with Dunnett’s multiple comparisons test (**P < 0.01, ***P < 0.001). G Flow cytometry analysis for apoptotic rates in U87 cells subjected to the indicated treatments.