Alt-R™ HDR Enhancer V2 from Integrated DNA Technologies

Product Specs
ItemAlt-R™ HDR Enhancer V2
CompanyIntegrated DNA Technologies
Price Supplier Page View Company Product Page
Catalog Number10007910
ApplicationsGene Expression and Molecular Cloning
TypeCRISPR/Cas9 Vectors

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Integrated DNA Technologies

1710 Commercial Park

Coralville, Iowa 52241

United States

Phone: 800-328-2661

Company Profile

Website: http://www.idtdna.com

(1) Acetyl-carnitine improves hyperactivity and learning deficits in KAT6A haploinsufficient miceLife Science AllianceMay 1, 2026Samantha Eccles, Hannah K Vanyai, Maria I Bergamasco, Shezlie Malelang, Havva Pehlivanoglu, Alexandra L Garnham, Nishika Ranathunga, Marnie E Blewitt, Adam P Vogel, Gordon K Smyth, et al.CRISPRMAX transfection reagent (CMAX00015; Thermo Fisher Scientific), and 0.55 μl Alt-R HDR Enhancer v2 (10007910; IDT) were added to the RNP complexes and incubated for 20 min at RT. HEK293T cells were diluted to

(2) HUH-tagged Cas9 as a platform for efficient ssODN-mediated knock-in via embryo and adult injection in insectsCommunications BiologyMarch 3, 2026Yu Shirai, Jonchee A. Kao, Tarun Kumar, Naoki Matsuda, Ryoya Nakagawa, Osamu Nureki, Cassandra G. Extavour, Takaaki DaimonsgRNA, and 0.7 μg/μL ssODNs with 1 mM MgCl2 and 500 mM NaCl. When using Alt-R HDR enhancer V2 (IDT, Cat#10007910), its concentration was adjusted to either 5 or 20 μM in the injection solution. Injected females were

(3) Unlocking CRISPR-Cas9 editing for widely diverse Dictyostelid speciesMolecular Systems BiologyJanuary 12, 2026Mireia Garriga-Canut, Nikki Cannon, Matt Benton, Andrea Zanon, Samuel T Horsfield, Jacob Scheurich, Kim Remans, John Lees, Alexandre Paix, Jordi van Gestelµg",IDT,1081058 "Alt-R Cas9 Electroporation Enhancer, 10 nmol",IDT,1075915 "Alt-R HDR Enhancer V2, 30 µL",IDT,10007910 "pHO4d-Cas9 (for SpyCas9 purification), see also Ref. (4)",Addgene,67881 TrueCut Cas9 Protein v2,ThermoFisher,A36498 QIAquick

(4) Epromoters bind key stress-related transcription factors to regulate clusters of stress response genesThe EMBO JournalJanuary 3, 2026Juliette Malfait, Jing Wan, Himanshu Narayan Singh, Charbel Souaid, Gaëlle Farah, Junhua Su, Magali Torres, Iris Manosalva, Nathalie Sakakini, Cyril Esnault, et al.µl of Alt-R® Cas9 Electroporation Enhancer (IDT, 075915), and 5 µl of Alt-R™ HDR Enhancer V2 (IDT, 10007910) using the Neon™ Transfection System (Thermo Fisher Scientific; 10 µl tips; pulse voltage 1450 V, pulse

(5) IFNγ Signaling Drives Resistance to FLT3 Inhibition in Acute Myeloid LeukemiaiScienceDecember 19, 2025Abraheem Khouqeer, Justin Cartailler, Christina Kim, Kirsten M. Dickerson, Chad R. Potts, Anna E. Gilbert, Robert S. Welner, P. Brent Ferrellsuspension, 12 μg of HDR plasmid concentrated in 2 μL and 1.9 μL of 100 μM Alt-R HDR Enhance r V2 (IDT, 10007910) were added. The cells were then mixed with the RNP complex. Electroporation was carried out using the

(6) Transcriptional cyclin-dependent kinases Cdk8 and Cdk19 are required for normal macrophage differentiationCell CycleDecember 17, 2025Aleksandra Kolodziejczyk, Samantha Liu, Fabian Strobel, Diego Martinez Martinez Alonso, Deborah Butter, Konrad Śnioch, Chen Chu, Henry Long, Veronika Sexl, Li Qiang, et al.were treated with a non-homologous end joining (NHEJ) inhibitor (Alt-R HDR Enhancer, IDT DNA, Cat. 10007910). Starting at 24 h after electroporation, cells were treated with puromycin for 48 h. ssDNA sequence

(7) CRISPR/methotrexate-integrated strategy for TCR-T cell engineering with reduced chromosome 14 lossMolecular Therapy Methods & Clinical DevelopmentDecember 11, 2025Jian Xu, Lianghua Shen, Ziyu Chen, Changmeng Zhang, Xiaodan Ding, Qiaomei He, Xiao Zhou, Peiyao Jiang, Jiayu Liu, Lu Li, et al.appropriate cytokines and transferred to a 48-well plate. In some experiments, Alt-R HDR enhancer v2 (IDT, 10007910) was used to enhance the proportion of cells undergoing HDR repair. Alt-R HDR enhancer v2 was added

(8) Resistance to CDK7 inhibitors directed by acquired mutation of a conserved residue in cancer cellsThe EMBO JournalSeptember 8, 2025Chun-Fui Lai, Victoria I Cushing, Ellen Olden, Charlotte L Bevan, R Charles Coombes, Basil J Greber, Laki Buluwela, Simak AliHY-103618 Palbociclib,Pfizer,Gifted Ribociclib,Selleckchem,Cat#S7440 Alt-R S.p. Cas9 Nuclease V3,IDT,Cat#1081058 Alt-R HDR Enhancer,IDT,Cat#10007910 NanoBRET TE Intracellular Kinase Assay K-10,Promega,Cat#N2642 SE Cell Line 4D-Nucleofector X Kit,Lonza,Cat#V4XC-1012 Xhol1,NEB,Cat#R0146S HiFi

(9) ONE-STEP tagging: a versatile method for rapid site-specific integration by simultaneous reagent deliveryNucleic acids researchAugust 11, 2025Migliori V, Bruntraeger MB, Gyulev IS, Lichou F, Burgold T, Gitterman DP, Iwama S, Trinh AL, Washer SJ, Jones CP, et al.sulfoxide (DMSO, Thermo Fisher Scientific) at a concentration of 5 mM. IDT HDR Enhancer v2 (IDT, cat. #10007910) was purchased as a 0.69 mM concentrated solution in DMSO. IDT HDR Enhancer v1 is no longer commercially

(10) Flower dependent trafficking of lamellar bodies facilitates maturation of the epidermal barrierNat CommunJuly 26, 2025Justin C. Rudd, Jos P. H. Smits, Patrick T. Kuwong, Rachel E. Johnson, Louise M. N. Monga, Ivonne M. J. J. van Vlijmen-Willems, Greer L. Porter, Peter O. Halloran, Kanika Sharma, Karina N. Schmidt, et al.above and seeded into fully supplemented EpiLife medium containing 1 µM Alt-R HDR Enhancer V2 (IDT 10007910). Cells were refed with enhancer-free EpiLife medium 16 h after electroporation. Sanger sequencing (Genewiz)

(11) Unraveling an enhancer-silencer regulatory element showing epistatic interaction with a variant that escaped genome-wide association studiesCell GenomicsJuly 9, 2025Mathieu Adjemout, Samia Nisar, Amélie Escandell, Romain Torres, Magali Torres, Hong Thu Nguyen Huu, Alassane Thiam, Iris Manosalva, Babacar Mbengue, Alioune Dieye, et al.Alt-R™-s.p Cas9 Nuclease V3,Integrated DNA technologies,Cat#1081059 Alt-R® HDR Enhancer V2,Integrated DNA technologies,Cat#10007910 DPBS,ThermoFisher,Cat#14190094 DMSO,Sigma-Aldrich,Cat#D4540 FBS,ThermoFisher,Cat#A5256701 ImmunoCult™-XF

(12) Generation of Plexin‐ B1 Conditional Knockout Mouse With CRISPR /Cas9 TechnologygenesisJune 27, 2025Haofei Ni, Kevin Kelley, Ning Xie, Hongyan Zou, Roland H. Friedeltesting of the impact of HDR enhancer on the viability of embryos, 1 μM of Alt-R HDR enhancer V2 (IDT, 10007910) was added for 24 hours to the media. 3.3 ∣ PCR genotyping Tail tips of mice (~1 mm) were boiled in

(13) NXPE1 alters the sialoglycome by acetylating sialic acids in the human colonNature CommunicationsMay 27, 2025Bum Seok Lee, Ashley Cook, Surojit Sur, Laura Dobbyn, Maria Popoli, Sana Khalili, Shibin Zhou, Chetan Bettegowda, Nickolas Papadopoulos, Kathy Gabrielson, et al.mixing 5 µL of RNP complex from above, 1.2 µL of HDR Donor Oligo, 1.2 µL of Alt-R™ HDR Enhancer V2 (IDT #10007910), 20 µL of the LS180 cell suspension in SG buffer (see above), and 0.1 µL of PBS (all reagents final

(14) Mechanism of ASF1 engagement by CDAN1Nat CommunMarch 16, 2025Samantha F. Sedor, Sichen Shaoaccording to the manufacturer’s instructions in media containing 690 nM Alt-R™ HDR Enhancer V2 (IDT, 10007910). After 48 hr, cells were placed under 2 μg/mL puromycin (Gibco, A11138) selection for an additional

(15) CEBPA repression by MECOM blocks differentiation to drive aggressive leukemiasbioRxivDecember 31, 2024Travis J. Fleming, Mateusz Antoszewski, Sander Lambo, Michael C. Gundry, Riccardo Piussi, Lara Wahlster, Sanjana Shah, Fiona E. Reed, Kevin D. Dong, Joao A. Paulo, et al.and supplemented with 50μl of crude rAAV HDR donor (see methods) and 1μM of alt-R HDR enhancer (IDT, 10007910). 24 hours later, cells were washed once with 1x PBS and replated in fresh cell culture medium. 72 hr

(16) CRISPR/Cas9 Ribonucleoprotein Nucleofection for Genome Editing in Primary Human Keratinocytes: Knockouts, Deletions, and Homology‐Directed Repair MutagenesisCurrent ProtocolsNovember 27, 2024Martina Bamundo, Sara Palumbo, Ludovica D'Auria, Caterina Missero, Daniela Di GirolamoHDR Donor Oligo, 2 nmol, lyophilized (IDT, www.idtdna.com)Alt‐R™ HDR Enhancer V2, 3 mM, 30 µl (IDT, 10007910)Alt‐R™ CRISPR‐Cas sgRNA (IDT, www.idtdna.com)Silicone grease (Molykote high‐vacuum silicone grease,

(17) protoSpaceJAM: an open-source, customizable and web-accessible design platform for CRISPR/Cas insertional knock-inNucleic Acids ResearchAugust 27, 2024Duo Peng, Madhuri Vangipuram, Joan Wong, Manuel D Leonettireagent (Stem Cell Technologies, #100-0691) and 1 μM HDR enhancer v2 (Integrated DNA Technologies #10007910), and they were maintained at 32°C and 5% CO2 for 2 days (cold shock). Cells were grown at 37°C and

(18) Non-canonical autophosphorylation of RIPK1 drives timely pyroptosis to control Yersinia infectionCell reportsAugust 27, 2024Jetton D, Muendlein HI, Connolly WM, Magri Z, Smirnova I, Batorsky R, Mecsas J, Degterev A, Poltorak A.1081058) together with Alt-R Cas9 Electroporation Enhancer (IDT, 1075915), Alt-R HDR Enhancer V2 (IDT, 10007910) the 80–120 bp ssDNA repair oligo (IDT) and the short guide RNA (sgRNA) comprised of both Alt-R CRISPR-Cas9 crRNA

(19) Mechanism of ASF1 Inhibition by CDAN1bioRxivAugust 14, 2024Samantha F. Sedor, Sichen Shaoaccording to the manufacturer’s instructions in media containing 690 nM Alt-R™ HDR Enhancer V2 (IDT, 10007910). After 48 hr, cells were placed under 2 μg/mL puromycin (Gibco, A11138) selection for an additional

(20) An autoinhibitory switch of the LSD1 disordered region controls enhancer silencingMolecular CellJune 20, 2024Amanda L. Waterbury, Hui Si Kwok, Ceejay Lee, Domenic N. Narducci, Allyson M. Freedy, Cindy Su, Shaunak Raval, Andrew H. Reiter, William Hawkins, Kwangwoon Lee, et al.Alt-R® Cas9 Electroporation Enhancer,Integrated DNA Technologies,Cat #1075915 Alt-R™ HDR Enhancer V2,Integrated DNA Technologies,Cat #10007910 Deposited data,Deposited data,Deposited data "ChIP-seq for LSD1, GFI1B, H3K27ac, RCOR1, PU.1, C/EBPα,

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Alt-R™ HDR Enhancer V2 from Integrated DNA Technologies

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Integrated DNA Technologies

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Alt-R™ HDR Enhancer V2 from Integrated DNA Technologies

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