Fig 1: Pharmacological or enzymatic inhibition of HBP1 methylation suppresses tumor cell metastasis and growth.A–H AMI-1 (Selleckchem, Cat#S7884) and MS023 (MedChemExpress, Cat#HY-19615) suppresses tumor cells metastasis and growth. A, B The protein levels of metastasis-related and proliferation-related markers were measured by western blotting in HeLa and MGC803 cells treated with AMI-1 (10 µM, 24 h) and MS023 (10 µM, 24 h). C, D Transwell assays were performed to test the migratory and invasive potential of the cells above (n = 5, Student’s t-test). Scale bar, 200 µm. E, F MTT (n = 3) and G, H EdU incorporation assays were performed to test the ability of cell growth in the cells above (n = 4, Student’s t-test). I–L G98R suppresses tumor cells metastasis and growth. I The protein levels of metastasis-related and proliferation-related markers were measured by western blotting in HeLa and MGC803 cells transfected with empty vector, PRMT1 or G98R. J Transwell assays were performed to test the migratory and invasive potential of the cells above (n = 4, one-way ANOVA). Scale bar, 200 µm. K MTT (n = 3, one-way ANOVA) and L EdU incorporation assays were performed to test the ability of cell growth in the cells above (n = 4, one-way ANOVA). Date were the mean ± SD. *p < 0.05, **p < 0.01.