Fig 1: CD146 expression was dependent on aryl hydrocarbon receptor (AhR)/ARNT signaling. (A) Immunofluorescence analysis revealed that AhR was translocated into the nucleus in MLE-12 cells treated with PM2.5, control: MLE-12 cells treated with PBS for 12 h; PM2.5: MLE-12 cells treated with 10 μg/ml PM2.5 for 12 h. (B) Western blot analysis of AhR, ARNT, and ARNT2 in MLE-12 cells treated with 10 μg/ml PM2.5. (C) Western blot analysis of CD146 in MLE-12 cells pretreated with 1 μm AHR inhibitor (PDM2, MedChemExpress, HY-112629) for 1 h and challenged with 10 μg/ml PM2.5 for 12 h. *P < 0.05; **P < 0.01; ns, not significant.
Fig 2: CD146 expression was dependent on aryl hydrocarbon receptor (AhR)/ARNT signaling. (A) Immunofluorescence analysis revealed that AhR was translocated into the nucleus in MLE-12 cells treated with PM2.5, control: MLE-12 cells treated with PBS for 12 h; PM2.5: MLE-12 cells treated with 10 μg/ml PM2.5 for 12 h. (B) Western blot analysis of AhR, ARNT, and ARNT2 in MLE-12 cells treated with 10 μg/ml PM2.5. (C) Western blot analysis of CD146 in MLE-12 cells pretreated with 1 μm AHR inhibitor (PDM2, MedChemExpress, HY-112629) for 1 h and challenged with 10 μg/ml PM2.5 for 12 h. *P < 0.05; **P < 0.01; ns, not significant.
Fig 3: CD146 expression was dependent on aryl hydrocarbon receptor (AhR)/ARNT signaling. (A) Immunofluorescence analysis revealed that AhR was translocated into the nucleus in MLE-12 cells treated with PM2.5, control: MLE-12 cells treated with PBS for 12 h; PM2.5: MLE-12 cells treated with 10 μg/ml PM2.5 for 12 h. (B) Western blot analysis of AhR, ARNT, and ARNT2 in MLE-12 cells treated with 10 μg/ml PM2.5. (C) Western blot analysis of CD146 in MLE-12 cells pretreated with 1 μm AHR inhibitor (PDM2, MedChemExpress, HY-112629) for 1 h and challenged with 10 μg/ml PM2.5 for 12 h. *P < 0.05; **P < 0.01; ns, not significant.
Fig 4: CD146 expression was dependent on aryl hydrocarbon receptor (AhR)/ARNT signaling. (A) Immunofluorescence analysis revealed that AhR was translocated into the nucleus in MLE-12 cells treated with PM2.5, control: MLE-12 cells treated with PBS for 12 h; PM2.5: MLE-12 cells treated with 10 μg/ml PM2.5 for 12 h. (B) Western blot analysis of AhR, ARNT, and ARNT2 in MLE-12 cells treated with 10 μg/ml PM2.5. (C) Western blot analysis of CD146 in MLE-12 cells pretreated with 1 μm AHR inhibitor (PDM2, MedChemExpress, HY-112629) for 1 h and challenged with 10 μg/ml PM2.5 for 12 h. *P < 0.05; **P < 0.01; ns, not significant.
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