Phosphatase Inhibitor Cocktail I (100× in DMSO) from MedChemExpress

Product Specs
ItemPhosphatase Inhibitor Cocktail I (100× in DMSO)
CompanyMedChemExpress
Price $45.00Supplier Page View Company Product Page
Catalog NumberHY-K0021
Quantity1 mL
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
Typekit

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(1) N-Terminal Basic Helix and S120 Phosphorylation Cooperatively Regulate Nuclear Localization of UBE2A/B in MechanotransductionBiology (Basel)September 16, 2026Mingwei Feng, Fumihiko Nakamuramin. For the phosphatase inhibitor treatment group, an additional phosphatase inhibitor cocktail (MCE, HY-K0021) was added to the TTBS lysis buffer during cell lysis and incubation. Bound FLAG-XPO4 was detected by

(2) Multi-omics and machine learning identify ALOX5 as a ferroptosis-associated driver of epithelial barrier dysfunction in chronic rhinosinusitis with nasal polypsWorld Allergy Organ JSeptember 7, 2026Ziheng Huang, Huiqin Zhou, Li Wang, Yuanyuan Yang, Xiaoqiang Chen, Chengxun Li, Hongmeng Yu, Desheng Wang(Beyotime, #P0013B) supplemented with protease inhibitor (MCE, HY-K0010) and phosphatase inhibitor (MCE, HY-K0021). Protein concentrations were determined using a BCA assay (Absin, #abs9232). The lysates were mixed

(3) A functional genomics-pharmacotranscriptomics framework identifies host-directed anti-influenza agentsiScienceAugust 21, 2026Jianfa Qiu, Xuecong Xing, Jingfeng Wang, Weijing Yuan, Xiaorong Li, Aiping Wu, Zhimin Gu, Zhuo Zhou, Jianwei Wang#P0013C) supplemented with 1% (vol/vol) proteinase and phosphatase inhibitor cocktail (MCE, #HY-K0010, #HY-K0021, #HY-K0022). The total protein was quantified using the BCA kit (Thermo Fisher Scientific, #23227) and

(4) Mononuclear phagocyte-specific cGAS/STING targeting suppresses experimental choroidal neovascularizationJCI InsightAugust 10, 2026Le Shi, Durgadas Cherukaraveedu, Hongkwan Cho, Kaoru Ri, Lingli Zhou, Yingxue Cao, Narendra Kale, Zhenhua Xu, Wathsala Liyanage, Rangaramanujam M. Kannan, et al.supplemented with a protease inhibitor cocktail (P8340, Sigma-Aldrich) and a phosphatase inhibitor cocktail (HY-K0021, MCE). The lysates were further disrupted by sonication and centrifuged to remove debris, after which

(5) NDP-MSH rescues LPS-induced neuroinflammation, synaptic deficits, and depressive-like behaviors in mice: involvement of MC1R-cAMP/PKA signalingFront PharmacolAugust 5, 2026Shanglan Qu, Xin Peng, Jieyu Ji, Ershu He, Le Jiang, Ruixue Ma, Hanwei Li, Yanjiao Li, Lijuan Li, Hui-Yin Yow, et al.11836153001) and phosphatase inhibitor (MedChemExpress, Monmouth Junction, NJ, United States; cat. no. HY-K0021, cat. no. HY-K0023). After incubation on ice for 30 min, lysates were centrifuged at 12,000 × g for

(6) Astrocytic LMP2 Coordinates NF-κB and TGF-β1/Smad3 Signaling to Drive Neuroinflammation after Cerebral Ischemia/ReperfusionAdv Sci (Weinh)July 27, 2026Yanguang Mao, Rulan Ma, Zejing Lin, Huiying Zhao, Yuexian Liu, Yonghe Su, Xu Zhang, Xingyong Chena complete protease inhibitor cocktail (Bimake, B142002) and phosphatase inhibitors (phosSTOP; MCE, HY‐K0021, HY‐K0022). Cell samples were washed twice with ice‐cold PBS, collected by scraping, and lysed on ice

(7) Gut microbiota translocation contributes to early islet apoptosis in streptozotocin-induced diabetesmSystemsJuly 21, 2026Ying Wang, Peng Sheng, Shijia Wang, Xiaohui Zhong, Hong Cao, Dan Li, Jiai Yan, Ju Yang, Yingyu Wang, Jie Peng, et al.China) containing 1% PMSF (ST507, Beyotime, Shanghai, China) and 1% Phosphatase Inhibitor Cocktail I (HY-K0021, MedChemExpress, Shanghai, China). Then, the lysate was centrifuged at 12,000 × g, 4°C for 20 min to

(8) RPLP2 Mediates the Beneficial Effects of Exercise on Stress Resistance Through Muscle-Brain CommunicationAdv Sci (Weinh)July 9, 2026Peiyu Luo, Wei Wu, Huan Peng, Dan He, Yuxi Guo, Boyue Zhao, Xiaodan Wang, Li Ma, Yuhang Qin, Yifang Zhai, et al.and tissues were sonicated in a buffer solution containing phosphatase and protease inhibitors (MCE, HY‐K0021 and HY‐K0011, respectively) for preparing a cell suspension. The samples were then centrifuged at 12,000

(9) Nicotinamide N-Methyltransferase Epigenetically Activates Fibronectin 1 Through H3K9me3 Remodeling in Clear Cell Renal Cell CarcinomaMedComm (2020)July 5, 2026Lingling Wang, Yueyang Wang, Qizheng Han, Xiao Zhou, Chenxia Wu, Honghe Zhang, Zhiyong Liang, Maode Laipresented using the 2−ΔΔCt method, though normalizing to β‐Actin. 5.6 Western Blotting Cocktail (MCE, HY‐K0021, HY‐K0022, HY‐K0010) and RIPA buffer (Beyotime, P0013B) were added to the cells. For the MNAM (Sigma‐Aldrich,

(10) SMARCA4 deficiency contributes to platinum resistance in non-small cell lung cancer by activating the NF-κB-BIRC2/BIRC3 signaling axisJournal of Thoracic DiseaseJune 30, 2026Yufeng Li, Na Zhou, Lin Zhang, Xingfa Huo, Yuming Zhang, Fangfang Yang, Xuchen Zhang, Chuantao Zhang, Dantong Sun, Helei Houprotease inhibitor cocktail (EDTA-free, 100× in DMSO; HY-K0010, MedChemExpress, Shanghai, China), phosphatase inhibitor cocktail I (100× in DMSO; HY-K0021, MedChemExpress, Shanghai, China), puromycin (E607054, Sangon Biotech, Shanghai, China), and a Cell

(11) SCP4 dephosphorylates mitotic histone H3 to maintain chromosome stabilityEMBO ReportsJune 19, 2026Yupiao Zheng, Zhengmao Zhang, Qi Wang, Jin Cao, Yang Li, Tingbo Liang, Xin-Hua Feng, Xia Lin(CHN),s805275,s805275 Nocodozole,Sigma-Aldrich,SML1665,SML1665 NotI-HF,NEB,R3189,R3189 PFA,Beyotime (CHN),P0099,P0099 Phosphatase Inhibitor Cocktail I,MedChemExpress,HY-K0021,HY-K0021 ProLong Gold mounting medium containing DAPI,Invitrogen,P36931,P36931 Protease Inhibitor Cocktail

(12) CTSZ-dependent anoikis resistance enhances malignant characters of glioblastoma via NF-κB signalingiScienceJune 19, 2026Yiyang Cao, Wei Wu, Yichang Wang, Beichen Zhang, Haoyu Zhou, Mingjing Zhou, Wei Chen, Maode Wang, Jia Wangbuffer (G2002, Servicebio Biological,China) containing protease and phosphatase inhibitors (HY-K0010/HY-K0021/HY-K0022, MedChemExpress, USA) for protein extraction. Protein concentration was quantified using a

(13) FGF13 Deficiency Ameliorates Paclitaxel‐Induced Neuropathic Pain by Inhibiting VASH1‐Mediated Microtubule Detyrosination to Promote MitophagyAdvanced ScienceJune 18, 2026Yiming Dong, Yidan Wang, Simeng Lv, Zishan Dong, Kaixi Zhi, Xiuhua Guo, Xuyan Li, Ruoxi Yu, Yiyi Zhang, Siyuan Cheng, et al.Shanghai, China; P0013) supplemented with phosphatase inhibitor cocktail (MedChemExpress, Newark, NJ, USA; HY‐K0021), and protease inhibitor cocktail (MedChemExpress, Newark, NJ, USA; HY‐K0010). The IP lysis buffer composition

(14) Protective Effects of Selective β-Adrenoceptor Blockade on Renal Pathophysiology in a Catecholamine Storm of RatInt J Mol SciJune 17, 2026Bo-Hau Chen, Tzu-Hao Liu, Guan-Hong Lin, Hsin-Hung Chen, Yi-Ting Chu, Chih-Chieh Yang, Wen-Hsien Lu(1:1000, HY-K0010, MedChemExpress, Monmouth Junction, NJ, USA), phosphatase inhibitor cocktail I (1:1000; HY-K0021, MedChemExpress, Monmouth Junction, NJ, USA), and phosphatase inhibitor cocktail II (1:1000, HY-K0022,

(15) Hepatic CREB Binding Protein/E1A Binding Protein p300 Maintain Bile Acid Homeostasis Through Histone Acetylation-Mediated Kruppel-Like Transcription Factor 10–Bile Salt Export Pump AxisCellular and Molecular Gastroenterology and HepatologyJune 10, 2026Mingzhu Li, Yiru Wang, Libin Zhou, Chunxiang Sheng, Ruyuan Deng, Li Shaoprotease inhibitor cocktail (MedChemExpress; HY-K0010), Phosphatase Inhibitor Cocktail I (MedChemExpress; HY-K0021), and Phosphatase Inhibitor Cocktail II (MedChemExpress; HY-K0022). For cultured cells, samples were

(16) The alternative splicing events and the function of TLN1 in clear cell renal cell carcinomaDiscover OncologyJune 8, 2026Xiaolei Xin, Zhengying Zhang, Xin Lan, Junting Li, Peiming Bai, Lin Fang, Wei Yan, Guangcheng Luowith both protease (Solarbio, cat. No. P0100) and phosphatase inhibitors (MedChemExpress, cat. No. HY-K0021). Protein concentrations were measured using the BCA protein assay kit (Sigma-Aldrich, cat. No. 71285-M).

(17) BAT-derived miR-378a-3p facilitates endothelial angiogenic function and promotes wound healingJCI InsightJune 8, 2026Hongyan Deng, Yuyu Xie, Jiadai Liu, Jing Ge, Qianqian Kang, Rui He, Zhihan Wang, Xuemin Peng, Zengzhe Zhu, Wenshe Wang, et al.supplemented with a proteinase inhibitor cocktail (HY-K0010, MCE) and phosphatase inhibitor cocktails (HY-K0021 and HY-K0022, MCE). Equal amounts of protein were separated on SDS-PAGE, and the proteins were subsequently

(18) The C9orf72/SMCR8 complex maintains microglial homeostasis via RAB8A-ESCRT-mediated lysosomal repairThe EMBO JournalMay 29, 2026Shan Li, Shidong Xu, Feng Li, Qirui Zhao, Penghui Zhang, Qinghua Guan, Xiangxiang Sun, Jundong Bi, Hu Xiao, Yiyuli Tang, et al.S HCl,Beyotime,ST018 Protease inhibitor cocktail,MCE,HY-K0011 Phosphatase inhibitor cocktail,MCE,HY-K0021 Dounce homogenizer (B pestle),Sigma,D8938 Pierce BCA Protein Assay kit,BIOMIKY,MK043A Mini-PROTEAN®

(19) Phenotypic Response Surfaces–Guided Optimization (PRS-OPT) of Propolis-Metformin-Regorafenib Combination Therapy for MASLD-Associated Hepatocellular CarcinomaOncology ResearchMay 21, 2026Yi-Sian Huang, Chung-Yung Ma, Hsiao-Yuh Roan, Cheng-Hsiung Chiang, Hsiao-Hui Tsou, Chen-Hui Chen, Yi-Fan Lin, Horng-Dar Wang, Chiou-Hwa Yuhinhibitor cocktail (MedChemExpress, HY-K0010) and phosphatase inhibitor cocktails (MedChemExpress, HY-K0021, HY-K0022, HY-K0023). Protein concentration was determined by BCA assay (Thermo Fisher Scientific Pierce™,

(20) Activation of PPARγ Attenuates Age-Related Lacrimal Gland Dysfunction by Alleviating Endoplasmic Reticulum Stress-Mediated FerroptosisInvest Ophthalmol Vis SciMay 20, 2026Han Zhao, Yiming Xu, Peiwen Feng, Xinyue Wang, Baihua Chen, Liwei Zhangradioimmunoprecipitation assay (RIPA) lysis buffer (#P0013C; Beyotime, Shanghai, China) with a phosphatase inhibitor cocktail (#HY-K0021; MedChemExpress, Monmouth Junction, NJ, USA). Protein concentrations of the lacrimal gland samples were

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Phosphatase Inhibitor Cocktail I (100× in DMSO) from MedChemExpress

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Phosphatase Inhibitor Cocktail I (100× in DMSO) from MedChemExpress

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