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Protein Biochemistry
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Substrate Reagent Pack
Substrate Reagent Pack from R&D Systems, a Bio-Techne Brand
Be the first to write a review!
Citations:
(132)
Supplier Page
Supplier Page from
R&D Systems, a Bio-Techne Brand for
Substrate Reagent Pack
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Description
New! 16 Vials TMB ELISA Substrate
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Substrate Reagent Pack
Company
R&D Systems, a Bio-Techne Brand
Price
$330.00
Supplier Page
View Company Product Page
Catalog Number
DY999B
Applications
ELISA
Quantity
1 Pack
Host
Human
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date
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R&D Systems, a Bio-Techne Brand
614 McKinley Place N.E.
Minneapolis
,
Minnesota
55413
United States
Phone
: 1 (800) 343-7475
1 (612) 379-2956
Fax: 1 (612) 656-4400
Company Profile
Website:
http://www.rndsystems.com
Citations (132)
(1)
IL1β+ macrophages promote intestinal fibrosis in Crohn’s disease by secreting AREG to activate PI16+ fibroblasts
Journal of Translational Medicine
March 14, 2026
Hanlin Xie, Xiaofeng Wen, Tao Ma, Lin Wang, Yanxu Guo, Guanzhan Liang, Jing Chen, Ruibing Li, Yuxi Pan, Zhenyu Xian,
et al.
using ELISACalc software. The ELISA kits used in this study were as follows:
DY262
,
DY008B
,
DY008C
,
DY999B
,
DY999
,
DY999B-250
(
R&D Systems
). Primary
human
intestinal
fibroblasts
isolation Three to four strips
(2)
Microbial metabolism of methotrexate produces a STAT3 signaling molecule that alleviates gut inflammation
bioRxiv
March 14, 2026
Juyeong Cho, Rebecca Danner, Woo Sung Kim, Jonathan Williams, Anshit Singh, Jiyoung A. Han, Jonathan G. Van Vranken, Allison S. Walker, Timothy Rhoads, Snehal N. Chaudhari
sequentially applied with intermediate washing steps. Signal was developed using TMB substrate (
R&D Systems
, #
DY999B
) and stopped with stop solution (
R&D Systems
, #
DY994
). Absorbance was measured at 450 nm with wavelength
(3)
Glycosaminoglycan-Inspired Polymer Brushes Promote Human Mesenchymal Stem Cell Proliferation While Retaining Trilineage Differentiation Potential
ACS Applied Materials & Interfaces
January 28, 2026
Claire L. Nelson, Caitlyn A. Castellion, Adam P. Humpal, Ram Prasad Sekar, Niraj Chaudhary, Luis G. Villa-Diaz, Ramya Kumar
washed 5 × 5 min. Then, we incubated wells with 100 μ L of tetramethylbenzidine solution (TMB, catalog #
DY999B
,
R&D Systems
) for 10 min in the dark and then added 50 μ L of stop solution. Plate reader measurements
(4)
Modulation of the PTPRS proteoglycan switch by antibodies binding to the membrane-proximal fibronectin-type III domain
Journal of Biological Chemistry
July 10, 2025
Thales Hein Da Rosa, Sterling H. Ramsey, Judy J. Lee, Natalia Y. Kozlova, Zixuan Zhao, Jaeyeon Kim, Ava C. Schneider, Sheng Li, Madhusudhanarao Katiki, Gary S. Firestein,
et al.
(
R&D Systems
,
HAF109
) for 1 h at room temperature. After washing, tetramethylbenzidine solution (
R&D Systems
,
DY999B
) was added to the wells and the absorbance at 450 nm was measured after the appropriate time using a
(5)
ApoE3 Christchurch and tau interaction as a protective mechanism against Alzheimer's disease
Alzheimers Dement
July 10, 2025
Paula Perez-Corredor, Said Arevalo-Alquichire, Randall C. Mazzarino, Michael O'Hare, Andres F. Muriel-Torres, Guido N. Vacano, Timothy E. Vanderleest, William P. Miller, Lina Pineda-Lopez, Shivani Patel,
et al.
the plate for 20 min in the dark with 3,3′5,5′‐tetramethylbenzidine substrate (TMD;
R&D Systems
, cat.
DY999B
). Between steps, plates were washed three times with TBS‐T enriched with 2 mM CaCl2. Optical densities
(6)
CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastoma
Nature communications
August 21, 2024
Pascual-Pasto G, McIntyre B, Hines MG, Giudice AM, Garcia-Gerique L, Hoffmann J, Mishra P, Matlaga S, Lombardi S, Shraim R,
et al
.
(7)
A plug-and-play, easy-to-manufacture fluidic accessory to significantly enhance the sensitivity of electrochemical immunoassays
Scientific reports
June 19, 2024
Dobrea A, Hall N, Milne S, Corrigan DK, Jimenez M.
kit (
DY206
). Reagent diluent x10 (1%
BSA
in PBS, pH 7.2-7.4, 0.2 µm filtered,
DY008B
), TMB substrate (
DY999B
) and PBS plate-coating buffer (DY006) were also purchased from the same manufacturer. The Au electrodes
(8)
Early Detection of Myeloid-Derived Suppressor Cells in the Lung Pre-Metastatic Niche by Shortwave Infrared Nanoprobes
Pharmaceutics
April 17, 2024
Siebert JN, Shah JV, Tan MC, Riman RE, Pierce MC, Lattime EC, Ganapathy V, Moghe PV.
(9)
H7N7 viral infection elicits pronounced, sex-specific neuroinflammatory responses in vitro
Frontiers in cellular neuroscience
January 1, 2024
Gabele L, Bochow I, Rieke N, Sieben C, Michaelsen-Preusse K, Hosseini S, Korte M.
(10)
MANF regulates neuronal survival and UPR through its ER-located receptor IRE1α
Cell reports
February 28, 2023
Kovaleva V, Yu LY, Ivanova L, Shpironok O, Nam J, Eesmaa A, Kumpula EP, Sakson S, Toots U, Ustav M,
et al
.
(11)
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Science advances
January 27, 2023
Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q.
(12)
Does the Blood-Brain Barrier Integrity Change in Regard to the Onset of Fetal Growth Restriction?
International journal of molecular sciences
January 19, 2023
Misan N, Michalak S, Kapska K, Osztynowicz K, Ropacka-Lesiak M, Kawka-Paciorkowska K.
(13)
Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucoma
Scientific reports
November 30, 2022
Rodrigo MJ, Bravo-Osuna I, Subias M, Montolío A, Cegoñino J, Martinez-Rincón T, Mendez-Martinez S, Aragón-Navas A, Garcia-Herranz D, Pablo LE,
et al
.
(14)
Dual Targeting of Soluble Oligomeric and Aggregated Transthyretin with a Monoclonal Antibody Ameliorates Experimental Neuropathy
Biology
October 15, 2022
Fassler M, Tshori S, Barac Y, Bowles DE, Benaim C, George J.
(15)
A TGFβR inhibitor represses keratin-7 expression in 3D cultures of human salivary gland progenitor cells
Scientific reports
September 2, 2022
Fowler EW, van Venrooy EJ, Witt RL, Jia X.
(16)
A pilot study on nitration/dysfunction of NK1 segment of myogenic stem cell activator HGF
Biochemistry and biophysics reports
September 1, 2022
Elgaabari A, Imatomi N, Kido H, Seki M, Tanaka S, Matsuyoshi Y, Nakashima T, Sawano S, Mizunoya W, Suzuki T,
et al
.
(17)
Cardioprotective effects of exercise preconditioning on ischemia-reperfusion injury and ventricular ectopy in young and senescent rats
Experimental gerontology
June 1, 2022
Fatahi A, Zarrinkalam E, Azizbeigi K, Ranjbar K.
(18)
Altered IgG glycosylation at COVID-19 diagnosis predicts disease severity
European journal of immunology
June 1, 2022
Vicente MM, Alves I, Gaifem J, Rodrigues CS, Fernandes Â, Dias AM, Štambuk J, Petrović T, Oliveira P, Ferreira-da-Silva F,
et al
.
(19)
Novel efficacious microRNA-30c analogs reduce apolipoprotein B secretion in human hepatoma and primary hepatocyte cells
The Journal of biological chemistry
April 1, 2022
Yadav PK, Haruehanroengra P, Irani S, Wang T, Ansari A, Sheng J, Hussain MM.
(20)
Quantifying Neutralizing Antibodies in Patients with COVID-19 by a Two-Variable Generalized Additive Model
mSphere
February 23, 2022
Liu KT, Gong YN, Huang CG, Huang PN, Yu KY, Lee HC, Lee SC, Chiang HJ, Kung YA, Lin YT,
et al
.
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Reviews
Be the First to Write a Review!
Substrate Reagent Pack from R&D Systems, a Bio-Techne Brand
Supplier Page
View Company Product Page
$330.00
Product Specs
Company Info
Citations
Reviews
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