Substrate Reagent Pack from R&D Systems, a Bio-Techne Brand

Product Specs
ItemSubstrate Reagent Pack
CompanyR&D Systems, a Bio-Techne Brand
Price $330.00Supplier Page View Company Product Page
Catalog NumberDY999B
ApplicationsELISA
Quantity1 Pack
HostHuman
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) IL1β+ macrophages promote intestinal fibrosis in Crohn’s disease by secreting AREG to activate PI16+ fibroblastsJournal of Translational MedicineMarch 14, 2026Hanlin Xie, Xiaofeng Wen, Tao Ma, Lin Wang, Yanxu Guo, Guanzhan Liang, Jing Chen, Ruibing Li, Yuxi Pan, Zhenyu Xian, et al.using ELISACalc software. The ELISA kits used in this study were as follows: DY262, DY008B, DY008C, DY999B, DY999, DY999B-250 (R&D Systems). Primary human intestinal fibroblasts isolation Three to four strips

(2) Microbial metabolism of methotrexate produces a STAT3 signaling molecule that alleviates gut inflammationbioRxivMarch 14, 2026Juyeong Cho, Rebecca Danner, Woo Sung Kim, Jonathan Williams, Anshit Singh, Jiyoung A. Han, Jonathan G. Van Vranken, Allison S. Walker, Timothy Rhoads, Snehal N. Chaudharisequentially applied with intermediate washing steps. Signal was developed using TMB substrate (R&D Systems, #DY999B) and stopped with stop solution (R&D Systems, #DY994). Absorbance was measured at 450 nm with wavelength

(3) Glycosaminoglycan-Inspired Polymer Brushes Promote Human Mesenchymal Stem Cell Proliferation While Retaining Trilineage Differentiation PotentialACS Applied Materials & InterfacesJanuary 28, 2026Claire L. Nelson, Caitlyn A. Castellion, Adam P. Humpal, Ram Prasad Sekar, Niraj Chaudhary, Luis G. Villa-Diaz, Ramya Kumarwashed 5 × 5 min. Then, we incubated wells with 100 μ L of tetramethylbenzidine solution (TMB, catalog # DY999B, R&D Systems) for 10 min in the dark and then added 50 μ L of stop solution. Plate reader measurements

(4) Modulation of the PTPRS proteoglycan switch by antibodies binding to the membrane-proximal fibronectin-type III domainJournal of Biological ChemistryJuly 10, 2025Thales Hein Da Rosa, Sterling H. Ramsey, Judy J. Lee, Natalia Y. Kozlova, Zixuan Zhao, Jaeyeon Kim, Ava C. Schneider, Sheng Li, Madhusudhanarao Katiki, Gary S. Firestein, et al.(R&D Systems, HAF109) for 1 h at room temperature. After washing, tetramethylbenzidine solution (R&D Systems, DY999B) was added to the wells and the absorbance at 450 nm was measured after the appropriate time using a

(5) ApoE3 Christchurch and tau interaction as a protective mechanism against Alzheimer's diseaseAlzheimers DementJuly 10, 2025Paula Perez-Corredor, Said Arevalo-Alquichire, Randall C. Mazzarino, Michael O'Hare, Andres F. Muriel-Torres, Guido N. Vacano, Timothy E. Vanderleest, William P. Miller, Lina Pineda-Lopez, Shivani Patel, et al.the plate for 20 min in the dark with 3,3′5,5′‐tetramethylbenzidine substrate (TMD; R&D Systems, cat. DY999B). Between steps, plates were washed three times with TBS‐T enriched with 2 mM CaCl2. Optical densities

(6) CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastomaNature communicationsAugust 21, 2024Pascual-Pasto G, McIntyre B, Hines MG, Giudice AM, Garcia-Gerique L, Hoffmann J, Mishra P, Matlaga S, Lombardi S, Shraim R, et al.

(7) A plug-and-play, easy-to-manufacture fluidic accessory to significantly enhance the sensitivity of electrochemical immunoassaysScientific reportsJune 19, 2024Dobrea A, Hall N, Milne S, Corrigan DK, Jimenez M.kit (DY206). Reagent diluent x10 (1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered, DY008B), TMB substrate (DY999B) and PBS plate-coating buffer (DY006) were also purchased from the same manufacturer. The Au electrodes

(8) Early Detection of Myeloid-Derived Suppressor Cells in the Lung Pre-Metastatic Niche by Shortwave Infrared NanoprobesPharmaceuticsApril 17, 2024Siebert JN, Shah JV, Tan MC, Riman RE, Pierce MC, Lattime EC, Ganapathy V, Moghe PV.

(9) H7N7 viral infection elicits pronounced, sex-specific neuroinflammatory responses in vitroFrontiers in cellular neuroscienceJanuary 1, 2024Gabele L, Bochow I, Rieke N, Sieben C, Michaelsen-Preusse K, Hosseini S, Korte M.

(10) MANF regulates neuronal survival and UPR through its ER-located receptor IRE1αCell reportsFebruary 28, 2023Kovaleva V, Yu LY, Ivanova L, Shpironok O, Nam J, Eesmaa A, Kumpula EP, Sakson S, Toots U, Ustav M, et al.

(11) Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesiclesScience advancesJanuary 27, 2023Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q.

(12) Does the Blood-Brain Barrier Integrity Change in Regard to the Onset of Fetal Growth Restriction?International journal of molecular sciencesJanuary 19, 2023Misan N, Michalak S, Kapska K, Osztynowicz K, Ropacka-Lesiak M, Kawka-Paciorkowska K.

(13) Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucomaScientific reportsNovember 30, 2022Rodrigo MJ, Bravo-Osuna I, Subias M, Montolío A, Cegoñino J, Martinez-Rincón T, Mendez-Martinez S, Aragón-Navas A, Garcia-Herranz D, Pablo LE, et al.

(14) Dual Targeting of Soluble Oligomeric and Aggregated Transthyretin with a Monoclonal Antibody Ameliorates Experimental NeuropathyBiologyOctober 15, 2022Fassler M, Tshori S, Barac Y, Bowles DE, Benaim C, George J.

(15) A TGFβR inhibitor represses keratin-7 expression in 3D cultures of human salivary gland progenitor cellsScientific reportsSeptember 2, 2022Fowler EW, van Venrooy EJ, Witt RL, Jia X.

(16) A pilot study on nitration/dysfunction of NK1 segment of myogenic stem cell activator HGFBiochemistry and biophysics reportsSeptember 1, 2022Elgaabari A, Imatomi N, Kido H, Seki M, Tanaka S, Matsuyoshi Y, Nakashima T, Sawano S, Mizunoya W, Suzuki T, et al.

(17) Cardioprotective effects of exercise preconditioning on ischemia-reperfusion injury and ventricular ectopy in young and senescent ratsExperimental gerontologyJune 1, 2022Fatahi A, Zarrinkalam E, Azizbeigi K, Ranjbar K.

(18) Altered IgG glycosylation at COVID-19 diagnosis predicts disease severityEuropean journal of immunologyJune 1, 2022Vicente MM, Alves I, Gaifem J, Rodrigues CS, Fernandes Â, Dias AM, Štambuk J, Petrović T, Oliveira P, Ferreira-da-Silva F, et al.

(19) Novel efficacious microRNA-30c analogs reduce apolipoprotein B secretion in human hepatoma and primary hepatocyte cellsThe Journal of biological chemistryApril 1, 2022Yadav PK, Haruehanroengra P, Irani S, Wang T, Ansari A, Sheng J, Hussain MM.

(20) Quantifying Neutralizing Antibodies in Patients with COVID-19 by a Two-Variable Generalized Additive ModelmSphereFebruary 23, 2022Liu KT, Gong YN, Huang CG, Huang PN, Yu KY, Lee HC, Lee SC, Chiang HJ, Kung YA, Lin YT, et al.

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Substrate Reagent Pack from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Substrate Reagent Pack from R&D Systems, a Bio-Techne Brand

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