TMB Substrate Pack, 250 mL from R&D Systems, a Bio-Techne Brand

Product Specs
ItemTMB Substrate Pack, 250 mL
CompanyR&D Systems, a Bio-Techne Brand
Price $330.00Supplier Page View Company Product Page
Catalog NumberDY999B
ApplicationsELISA
Quantity1 Pack
HostHuman
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date

Specifications/Features

Available conjugates: Sizes Available: 1 Pack

Add to Compare List

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Phased fragility and stability of non-genetic B cell states in the germinal center accelerate the genetic evolution of antibodiesCell SystemsApril 20, 2026Mark Y. Xiang, Haripriya Vaidehi Vaidehi Narayanan, Vaibhava Kesarwani, Rohan Vanheusden, Tiffany Wang, Alexander HoffmannKit,ThermoFisher Scientific,C34564 Streptavidin-HRP,R&D Systems,DY998 TMB color reagent for ELISA,R&D Systems,DY999B Deposited Data,Deposited Data,Deposited Data ELISA,This Study,DOI: 10.5281/zenodo.18778833 FLOW,This

(2) Ectopic NMDAR expression in cancer unmasks germline-encoded autoimmunityNatureMarch 25, 2026Sam O. Kleeman, Kevin Michalski, Xiang Zhao, Ruben Steigerwald, Miriam Ferrer, Llewelyn Levett, Ethan Ertel, Austin Schultz, Noriko Simorowski, Pamela Moody, et al.buffer to reduce non-specific signals. To visualize the signal, 100 μl of TMB buffer (R&D Systems, DY999B) was added for 20 min, after which time the reaction was stopped with 50 μl of stop solution (R&D Systems,

(3) IL1β+ macrophages promote intestinal fibrosis in Crohn’s disease by secreting AREG to activate PI16+ fibroblastsJournal of Translational MedicineMarch 14, 2026Hanlin Xie, Xiaofeng Wen, Tao Ma, Lin Wang, Yanxu Guo, Guanzhan Liang, Jing Chen, Ruibing Li, Yuxi Pan, Zhenyu Xian, et al.using ELISACalc software. The ELISA kits used in this study were as follows: DY262, DY008B, DY008C, DY999B, DY999, DY999B-250 (R&D Systems). Primary human intestinal fibroblasts isolation Three to four strips

(4) Microbial metabolism of methotrexate produces a STAT3 signaling molecule that alleviates gut inflammationbioRxivMarch 14, 2026Juyeong Cho, Rebecca Danner, Woo Sung Kim, Jonathan Williams, Anshit Singh, Jiyoung A. Han, Jonathan G. Van Vranken, Allison S. Walker, Timothy Rhoads, Snehal N. Chaudharisequentially applied with intermediate washing steps. Signal was developed using TMB substrate (R&D Systems, #DY999B) and stopped with stop solution (R&D Systems, #DY994). Absorbance was measured at 450 nm with wavelength

(5) Glycosaminoglycan-Inspired Polymer Brushes Promote Human Mesenchymal Stem Cell Proliferation While Retaining Trilineage Differentiation PotentialACS Applied Materials & InterfacesJanuary 28, 2026Claire L. Nelson, Caitlyn A. Castellion, Adam P. Humpal, Ram Prasad Sekar, Niraj Chaudhary, Luis G. Villa-Diaz, Ramya Kumarwashed 5 × 5 min. Then, we incubated wells with 100 μ L of tetramethylbenzidine solution (TMB, catalog # DY999B, R&D Systems) for 10 min in the dark and then added 50 μ L of stop solution. Plate reader measurements

(6) Modulation of the PTPRS proteoglycan switch by antibodies binding to the membrane-proximal fibronectin-type III domainJournal of Biological ChemistryJuly 10, 2025Thales Hein Da Rosa, Sterling H. Ramsey, Judy J. Lee, Natalia Y. Kozlova, Zixuan Zhao, Jaeyeon Kim, Ava C. Schneider, Sheng Li, Madhusudhanarao Katiki, Gary S. Firestein, et al.(R&D Systems, HAF109) for 1 h at room temperature. After washing, tetramethylbenzidine solution (R&D Systems, DY999B) was added to the wells and the absorbance at 450 nm was measured after the appropriate time using a

(7) ApoE3 Christchurch and tau interaction as a protective mechanism against Alzheimer's diseaseAlzheimers DementJuly 10, 2025Paula Perez-Corredor, Said Arevalo-Alquichire, Randall C. Mazzarino, Michael O'Hare, Andres F. Muriel-Torres, Guido N. Vacano, Timothy E. Vanderleest, William P. Miller, Lina Pineda-Lopez, Shivani Patel, et al.the plate for 20 min in the dark with 3,3′5,5′‐tetramethylbenzidine substrate (TMD; R&D Systems, cat. DY999B). Between steps, plates were washed three times with TBS‐T enriched with 2 mM CaCl2. Optical densities

(8) CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastomaNature communicationsAugust 21, 2024Pascual-Pasto G, McIntyre B, Hines MG, Giudice AM, Garcia-Gerique L, Hoffmann J, Mishra P, Matlaga S, Lombardi S, Shraim R, et al.

(9) A plug-and-play, easy-to-manufacture fluidic accessory to significantly enhance the sensitivity of electrochemical immunoassaysScientific reportsJune 19, 2024Dobrea A, Hall N, Milne S, Corrigan DK, Jimenez M.kit (DY206). Reagent diluent x10 (1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered, DY008B), TMB substrate (DY999B) and PBS plate-coating buffer (DY006) were also purchased from the same manufacturer. The Au electrodes

(10) Early Detection of Myeloid-Derived Suppressor Cells in the Lung Pre-Metastatic Niche by Shortwave Infrared NanoprobesPharmaceuticsApril 17, 2024Siebert JN, Shah JV, Tan MC, Riman RE, Pierce MC, Lattime EC, Ganapathy V, Moghe PV.

(11) H7N7 viral infection elicits pronounced, sex-specific neuroinflammatory responses in vitroFrontiers in cellular neuroscienceJanuary 1, 2024Gabele L, Bochow I, Rieke N, Sieben C, Michaelsen-Preusse K, Hosseini S, Korte M.

(12) MANF regulates neuronal survival and UPR through its ER-located receptor IRE1αCell reportsFebruary 28, 2023Kovaleva V, Yu LY, Ivanova L, Shpironok O, Nam J, Eesmaa A, Kumpula EP, Sakson S, Toots U, Ustav M, et al.

(13) Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesiclesScience advancesJanuary 27, 2023Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q.

(14) Does the Blood-Brain Barrier Integrity Change in Regard to the Onset of Fetal Growth Restriction?International journal of molecular sciencesJanuary 19, 2023Misan N, Michalak S, Kapska K, Osztynowicz K, Ropacka-Lesiak M, Kawka-Paciorkowska K.

(15) Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucomaScientific reportsNovember 30, 2022Rodrigo MJ, Bravo-Osuna I, Subias M, Montolío A, Cegoñino J, Martinez-Rincón T, Mendez-Martinez S, Aragón-Navas A, Garcia-Herranz D, Pablo LE, et al.

(16) Dual Targeting of Soluble Oligomeric and Aggregated Transthyretin with a Monoclonal Antibody Ameliorates Experimental NeuropathyBiologyOctober 15, 2022Fassler M, Tshori S, Barac Y, Bowles DE, Benaim C, George J.

(17) A TGFβR inhibitor represses keratin-7 expression in 3D cultures of human salivary gland progenitor cellsScientific reportsSeptember 2, 2022Fowler EW, van Venrooy EJ, Witt RL, Jia X.

(18) A pilot study on nitration/dysfunction of NK1 segment of myogenic stem cell activator HGFBiochemistry and biophysics reportsSeptember 1, 2022Elgaabari A, Imatomi N, Kido H, Seki M, Tanaka S, Matsuyoshi Y, Nakashima T, Sawano S, Mizunoya W, Suzuki T, et al.

(19) Altered IgG glycosylation at COVID-19 diagnosis predicts disease severityEuropean journal of immunologyJune 1, 2022Vicente MM, Alves I, Gaifem J, Rodrigues CS, Fernandes Â, Dias AM, Štambuk J, Petrović T, Oliveira P, Ferreira-da-Silva F, et al.

(20) Cardioprotective effects of exercise preconditioning on ischemia-reperfusion injury and ventricular ectopy in young and senescent ratsExperimental gerontologyJune 1, 2022Fatahi A, Zarrinkalam E, Azizbeigi K, Ranjbar K.

Be the First to Write a Review!

TMB Substrate Pack, 250 mL from R&D Systems, a Bio-Techne Brand

Description

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (134)

Reviews

TMB Substrate Pack, 250 mL from R&D Systems, a Bio-Techne Brand

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map