Alt-R™ Cas9 Electroporation Enhancer from Integrated DNA Technologies

Product Specs
ItemAlt-R™ Cas9 Electroporation Enhancer
CompanyIntegrated DNA Technologies
Price Supplier Page View Company Product Page
Catalog Number1075915
ApplicationsGene Expression and Molecular Cloning
TypeEnhancer

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Integrated DNA Technologies

1710 Commercial Park

Coralville, Iowa 52241

United States

Phone: 800-328-2661

Company Profile

Website: http://www.idtdna.com

(1) Mouse TAPBPR shows functional similarity to human TAPBPR in shaping the MHC-I immunopeptidomeFront ImmunolApril 15, 2026Juliana Bernardi Aggio, Marcel Wacker, William Guo, Arwen F. Altenburg, Ida Hafstrand, Reem Satti, Alison McDowall, Aure Aflalo, Andreas Neerincx, Jack Welland, et al.(washed three times in advance) in combination with 1 nmol of Alt-R Cas9 Electroporation Enhancer (CAT: 1075915, IDT), and 100 μL of Cell Line Nucleofector Kit V (Lonza) for MC-38 and B16-F10 cells, or Kit L for

(2) IFNγ-induced antigen loss in chimeric antigen receptor-T cell therapyFront ImmunolMarch 17, 2026Miao Cao, Jasmine Alvarez, Ramkrishna Mitra, Michael Xu, Trevor R. Baybutt, Zhengyang Sun, Oluwatobiloba Taylor, Allison S. Doermann, Ross E. Staudt, Scott A. Waldman, et al.supplement (Lonza, V4XC-1032) and then combined with 5 μL RNP and 1 μL electroporation enhancer (IDT, 1075915). Cells were then electroporated using a Lonza 4D-Nucleofector X unit (AAF-1003X) with the DS-150 program.

(3) IFNγ-induced antigen loss in chimeric antigen receptor-T cell therapyFrontiers in ImmunologyMarch 17, 2026Miao Cao, Jasmine Alvarez, Ramkrishna Mitra, Michael Xu, Trevor R. Baybutt, Zhengyang Sun, Oluwatobiloba Taylor, Allison S. Doermann, Ross E. Staudt, Scott A. Waldman, et al.supplement (Lonza, V4XC-1032) and then combined with 5 μL RNP and 1 μL electroporation enhancer (IDT, 1075915). Cells were then electroporated using a Lonza 4D-Nucleofector X unit (AAF-1003X) with the DS-150 program.

(4) HPV16 E6 oncoprotein promotes microhomology-mediated viral integration by increasing PolΘ protein expressionProc Natl Acad Sci U S AMarch 10, 2026Guangli Zhu, Shuhei Asada, Jithma P. Abeykoon, Lifang Sun, Sirisha Mukkavalli, Alan D. D'AndreaAlt-R® S.p. Cas9 Nuclease V3 (Integrated DNA Technologies, #1081059), and Enhancer (Integrated DNA Technologies, #1075915) were mixed and incubated at room temperature for 10 min to allow RNP formation. The RNP mixture was

(5) CRISPR-based functional genomics for dissecting therapeutic dependency in primary acute myeloid leukemia samplesMolecular CellMarch 5, 2026Zhendong Cao, Sixiang Yu, Jacqueline Peng, Declan R. Barrett, Yuqiao Liu, Jonathan H. Sussman, Changya Chen, Anusha Thadi, Li Liu, Fatemeh Alikarami, et al.T100B Alt-R™ S.p. Cas9 Nuclease V3,IDT,Cat # 1081058 Alt-R® Cas9 Electroporation Enhancer,IDT,Cat # 1075915 BsmBI-v2,NEB,Cat # R0739S Methylcellulose-based medium with recombinant cytokines for human cells,STEMCELL

(6) FOXA1 mutations co-opt nascent transcription factor networks in partnership with androgen receptor to enhance prostate tumorigenicityCell ReportsFebruary 24, 2026Erik M. Ladewig, Abbas Nazir, Tyler Park, Vinson B. Fan, Zhendong Cao, Jacob Hawk, Lauren Kelly, Robert Tjian, Christina S. Leslie, Charles L. Sawyerscells were resuspended with nucleofection buffer, RNP complexes, and electroporation enhancer (IDT 1075915, 1:1molar ratio to cRNP) in a total volume of 100 μL. The cell suspension was transferred to a nucleofection

(7) Chromatin-mediated anticipatory control of type I interferon production in plasmacytoid dendritic cellsImmunityFebruary 10, 2026Nicholas M. Adams, Aleksandra Galitsyna, Ioanna Tiniakou, Eduardo Esteva, Ai C. Ra, Simon Ullrich, Stephen T. Yeung, Yanjun Tan, Joseph N. Pucella, Igor Dolgalev, et al.proteins" β-Estradiol,Sigma-Aldrich,Cat# E2758 Alt-R S.p. Cas9 Nuclease V3,IDT,Cat# 1081058 Alt-R Cas9 Electroporation Enhancer,IDT,Cat# 1075915 (Z)-4-Hydroxytamoxifen,Sigma-Aldrich,Cat# H7904 Recombinant Mouse GM-CSF (carrier free),BioLegend,Cat#

(8) Unlocking CRISPR-Cas9 editing for widely diverse Dictyostelid speciesMolecular Systems BiologyJanuary 12, 2026Mireia Garriga-Canut, Nikki Cannon, Matt Benton, Andrea Zanon, Samuel T Horsfield, Jacob Scheurich, Kim Remans, John Lees, Alexandre Paix, Jordi van GestelAlt-R CRISPR-Cas9 crRNA,IDT,Custom "Alt-R S.p. Cas9 Nuclease V3, 100 µg",IDT,1081058 "Alt-R Cas9 Electroporation Enhancer, 10 nmol",IDT,1075915 "Alt-R HDR Enhancer V2, 30 µL",IDT,10007910 "pHO4d-Cas9 (for SpyCas9 purification), see also Ref.

(9) The transaminase-ω-amidase pathway senses oxidative stress to control glutamine metabolism and α-ketoglutarate levels in endothelial cellsThe EMBO JournalDecember 17, 2025Niklas Herrle, Pedro F Malacarne, Timothy Warwick, Alfredo Cabrera-Orefice, Yiheng Chen, Maedeh Gheisari, Souradeep Chatterjee, Matthias S Leisegang, Tamim Sarakpi, Sarah Wionski, et al.according to the manufacturer’s protocol (IDT, Belgium) with Alt-R Cas9 Electroporation Enhancer (#1075915, IDT, Belgium). The sgRNA and donor oligo sequence were designed with the Alt-R HDR Design Tool (IDT,

(10) Systematic Evaluation of GAPs and GEFs Identifies a Targetable Dependency for Hematopoietic MalignanciesCancer DiscoveryDecember 2, 2025Pu Zhang, Zhendong Cao, Xiangyu Pan, Yuqiao Liu, Cynthia Castro, Won Jun Kim, Takeshi Fujino, Jennifer Lewis, Jahan Rahman, Sanam Shahid, et al.T100B Alt-R™ S.p. Cas9 Nuclease V3,IDT,Cat # 1081058 Alt-R® Cas9 Electroporation Enhancer,IDT,Cat # 1075915 BsmBI-v2,New England Biolabs,Cat # R0739S "Rho Inhibitor, Rhosin - Calbiochem",EMD Millipore,Cat #

(11) Megabase-scale loss of heterozygosity provoked by CRISPR-Cas9 DNA double-strand breaksMolecular CellNovember 20, 2025Samantha B. Regan, Darpan Medhi, Yuanlin Xu, Travis B. White, Yi-Zhen Jiang, Jung Eun Kim, Shih-Chun Wang, Qichen Deng, Su Jia, Dulguun Baasan, et al.substrate,Revvity Health Sciences,Cat# NEL104001EA Cas9 electroporation enhancer,Integrated DNA Technologies,Cat# 1075915 Ethanol,Decon Laboratories,Cat# 2701 Propidium iodide,Millipore Sigma,Cat# P4864 Purelink Rnase A,Invitrogen,Cat#

(12) The transcriptional repressor BLIMP1 enforces TCF-1-dependent and -independent restriction of the memory fate of CD8+ T cellsImmunityOctober 14, 2025Maegan K. Murphy, Matthew McCullen, Joshua L. Deffenbaugh, Andy Y. Chen, Joy Pai, Bence Daniel, Amir Yousif, Saravanan Raju, Sunnie Hsiung, Zhenxiao Wang, et al.assays,Critical commercial assays,Critical commercial assays ,, Alt-R Cas9 Electroporation Enhancer,IDT,Cat#1075915 P3 Primary Cell 4D-Nucleofector® X Kit S,Lonza,Cat# V4XP-3032 Precision Count Beads,BioLegend,Cat

(13) Evidence for enhancer activity in intron 1 of TNFRSF1A using CRISPR/Cas9 in human induced pluripotent stem cell-derived macrophagesSci RepOctober 7, 2025Julie A. Osgood, Andrew C. Brown, Katie L. Burnham, Olga Mielczarek, Gabriele Migliorini, Chwen Tay, Ping Zhang, Madeleine H. Palmer, Ben Davies, Sally A. Cowley, et al.transfect, RNP transfection solution, 2.2 × 105 iPSCs and Alt-R® Cas9 Electroporation Enhancer (Catalog# 1075915, IDT) were mixed, added to the 10µL tip and transfected with the following conditions: 1250 V/20 ms/1

(14) Tumor-derived CCL2 drives tumor growth and immunosuppression in IDH1- mutant cholangiocarcinomaHepatologySeptember 1, 2025Daniel J. Zabransky, Emma Kartalia, Jae W. Lee, James M. Leatherman, Soren Charmsaz, Sara E. Young, Yash Chhabra, Sebastià Franch-Expósito, Martin Kang, Saumya Maru, et al.(5’-TCATTGGCCGACATGCATAT-3’) and Alt-R S.p. HiFi Cas9 enzyme (IDT 1081060), Alt-R Cas9 Electroporation Enhancer (IDT 1075915), and single-stranded oligodeoxynucleotides (ssODNs) to serve as homology-directed repair templates

(15) Development of an Anti-CD99 Antibody Enables Targeting of Diffuse Midline GliomaCancer ResearchAugust 15, 2025Ilango Balakrishnan, Krishna Madhavan, Angela Pierce, Joshua Michlin, Breauna Brunt, Senthilnath Lakshmanachetty, Dong Wang, John DeSisto, Zachary James Nuss, Nathan Davidson, et al.they were resuspended in 240-μL P3 Primary Cell Solution (Lonza); 15-μL Alt-R Cas9 enhancer (IDT, #1075915) and 90-μL RNP mixture were then added to the cell suspension. This cell suspension was transferred

(16) Single-protein/RNA imaging reveals ZNF598 as a limiting factor in resolving collided ribosomesThe EMBO JournalAugust 1, 2025Ana C De La Cruz, Garrett Tisdale, Emily Nakayama, Zhiyuan Huang, Niladri K Sinha, Rachel Green, Bin Wuwere added to RNP solution. In all, 2 µg of Donor DNA and 1 µL Cas9 Electroporation Enhancer (IDT # 1075915) were added to RNP cell mixture. Electroporation was performed according to the manufacturer’s instructions

(17) Single-cell analysis of temporal immune cell dynamics in alopecia areata reveals a causal role for clonally expanded CD8+ T cells in diseaseCell ReportsJuly 22, 2025Zhenpeng Dai, Yuqian Chang, Eunice Y. Lee, Valia P. Leifer, Eddy Hsi Chun Chun Wang, Rolando Perez-Lorenzo, Annemieke de Jong, Angela M. Christiano,Thermo Fisher Scientific,Cat# A50576 Alt-R Cas9 Electroporation Enhancer,Integrated DNA Technologies,Cat# 1075915 RetroNectin Recombinant Human Fibronectin Fragment,Takara Bio.,Cat# T100B "Collagenase, Type 4",Worthington,Cat#

(18) Unraveling an enhancer-silencer regulatory element showing epistatic interaction with a variant that escaped genome-wide association studiesCell GenomicsJuly 9, 2025Mathieu Adjemout, Samia Nisar, Amélie Escandell, Romain Torres, Magali Torres, Hong Thu Nguyen Huu, Alassane Thiam, Iris Manosalva, Babacar Mbengue, Alioune Dieye, et al.,Integrated DNA technologies,Cat#1081060 Alt-R® Cas9 Electroporation Enhancer 2 nM,Integrated DNA technologies,Cat#1075915 Alt-R® Cas9 Electroporation Enhancer 10 nM,Integrated DNA technologies,Cat#1075916 Alt-R™-s.p Cas9 Nuclease V3

(19) CREBBP inactivation sensitizes B cell acute lymphoblastic leukemia to ferroptotic cell death upon BCL2 inhibitionNat CommunMay 20, 2025Alicia Garcia-Gimenez, Jonathan E. Ditcham, Dhoyazan M. A. Azazi, George Giotopoulos, Ryan Asby, Eshwar Meduri, Jaana Bagri, Nathalie Sakakini, Cecile K. Lopez, Nisha Narayan, et al.homology directed repair enhancer (IDT, 1081072) and IDT nucleofection enhancer oligonucleotide (IDT 1075915) according to manufacturer’s protocol. At 48 h ATTO550+ve single cells were FACS sorted into 96-well

(20) Antagonistic roles for MITF and TFE3 in melanoma plasticityCell ReportsApril 22, 2025Jeremy Chang, Katelyn R. Campbell-Hanson, Marion Vanneste, Nicholas I. Bartschat, Ryan Nagel, Asdis K. Arnadottir, Hong Nhung Vu, Collin Montgomery, Julius Yevdash, Jiarui Jiang, et al.Gene Pulser electroporation buffer (#1652677, Bio-Rad, Hercules, CA) with electroporation enhancer (#1075915, IDT), using 140V and a 2mm pulse gap. A nontargeting negative control gRNA (#1072544, IDT) was used

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Alt-R™ Cas9 Electroporation Enhancer from Integrated DNA Technologies

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Integrated DNA Technologies

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Alt-R™ Cas9 Electroporation Enhancer from Integrated DNA Technologies

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