Spectral DAPI from Akoya Biosciences, a Quanterix company

Product Specs
ItemSpectral DAPI
CompanyAkoya Biosciences, a Quanterix company
Price $67.38Supplier Page View Company Product Page
Catalog NumberFP1490
Quantity1
ApplicationsMultiplex IHC

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Akoya Biosciences, a Quanterix company

900 Middlesex Turnpike

Building 1

Billerica, MA 01821

United States

Phone: +1 (617) 765-7943

Email: sales@quanterix.com

Company Profile

Website: quanterix.com

(1) The role of macrophages in the mitigation by decitabine of acute allograft rejectionFront TransplantDecember 11, 2025William N. Daccarett-Bojanini, Manuel Sollmann, Kristine M. Yarnoff, Nicola M. Heller, Jeffrey M. Dodd-OMA) detected with OPAL Polaris 780. Finally, sections were counterstained with spectral DAPI (Cat. #FP1490, Akoya Biosciences, Marlborough, MA) and mounted with Prolong Gold (Cat. #P36930, Thermo Fisher Scientific,

(2) CD47 expression in solid tumors correlates with phagocytic tumor-associated macrophage gene signatureFront ImmunolDecember 3, 2025Nicholas van Buuren, Mengshu Xu, Yi Zhang, Paola Correa, Shiva Zaboli, Azadeh Madjidi, Christina Moon, Szu-Wen Liu, Ruidong Li, Kai-Hui Sun, et al.dilution; and the same anti-pan-Cytokeratin described above at Position 4. Spectral DAPI (PerkinElmer FP1490, 2 drops/mL in TBS buffer) was used to counterstain. Slides were scanned to generate whole slide images

(3) Targeting PAK1 or PAK4 Uncovers Different Mechanisms of Vascular Reprogramming in Pancreatic CancerCellsNovember 17, 2025Arian Ansardamavandi, Chelsea Dumesny, Sarah Ellis, Ching-Seng Ang, Mehrdad Nikfarjam, Hong He(B40923; Thermo Fisher Scientific), and 488) (Table S4). Nuclear counterstaining was performed with DAPI (FP1490, Akoya Biosciences, Marlborough, MA, USA), and the slides were mounted with VECTASHIELD antifade medium

(4) TNFR1 Suppression by XPro1595 Reduces Peripheral Neuropathies Associated with Perineural Invasion in Female MiceCellsNovember 7, 2025Morgan Zhang, Naijiang Liu, Kesava Asam, Charles Meng, Bradley Aouizerat, Yi Yeantibodies. Sections were counterstained with spectral DAPI (Akoya Biosciences, Marlborough, MA, USA, FP1490) and mounted using ProLong Gold Antifade (ThermoFisher Scientific, Waltham, MA, USA, P36935). Semi-automated

(5) The Effects of PAK-Regulated Tumour Vasculature on Gemcitabine Response of Pancreatic CancerCancers (Basel)October 26, 2025Arian Ansardamavandi, Chelsea Dumesny, Yi Ma, Li Dong, Sarah Ellis, Ching-Seng Ang, Mehrdad Nikfarjam, Hong HeFluor™ 488 was applied second if Alexa Fluor™ 647 was used first. Nuclei were counterstained with DAPI (FP1490, Akoya Biosciences, Marlborough, MA, USA) and mounted in VECTASHIELD antifade medium (H-1700, Vector Laboratories

(6) Bcl6 expression is associated with a distinct immune landscape and spatial transcriptome in COVID-19JCI InsightOctober 22, 2025Cloé Brenna, Bernat Bramon Mora, Kalliopi Ioannidou, Julien Bodelet, Mia L. Siebmanns, Simon Burgermeister, Spiros Georgakis, Michail Orfanakis, Yannick D. Muller, Nazanin Sédille, et al.the final staining step, sections were counterstained with Spectral DAPI (Akoya Biosciences, catalog FP1490) to visualize nuclei. To ensure thorough reagent removal, slides were washed using a mild detergent

(7) T cell subsets of urine-derived lymphocytes (UDLs) serve as an indicator of TILs and reflect immunological sex differences in bladder cancerJ Immunother CancerOctober 2, 2025Johanna M Schafer, No-Joon Song, Tong Xiao, Timothy D Gauntner, Kyeong Joo Jung, Emily G Fitts, Krishan Kumar, Hyeong Seon Jeon, Ryan A Elaoud, Kelsi Reynolds, et al.each fluorophore conjugation step. Finally, spectral 4′,6-diamidino-2-phenylindole (DAPI) (Akoya, SKU FP1490) was applied according to the manufacturer’s recommendations, and slides were coverslipped using SlowFade Gold Antifade Mountant

(8) An injury-associated lobular microniche is associated with the classical tumor cell phenotype in pancreatic cancerNature communicationsSeptember 26, 2025Söderqvist S, Viljamaa A, Geyer N, Keller AL, Ruksha K, Strell C, Hekmati N, Niculae A, Engstrand J, Sparrelid E, et al.solution 1 (Leica Bond ER1, #AR9961) at 95 °C for 20 min. Finally, a 5 min DAPI (Akoya Biosciences, #FP1490) nuclear counterstaining was performed before mounting with ProLong Diamond Antifade media (Thermo Fisher Scientific

(9) Targeting DNA Polymerase Epsilon Leads to Tumor Clearance and Activation of an NF-κB-mediated inflammatory response in Triple Negative Breast CancerbioRxivSeptember 23, 2025Elizabeth F. Sher, Kenji M. Fujihara, Anthony Tao, Diana Erenburg, Vladislav O. Sviderskiy, Hannan Mir, Triantafyllia R. Karakousi, Cynthia Loomis, Jiehui Deng, Kwok-Kin Wong, et al.the Ki67 and Foxp3 antibodies). Sections were counterstained with spectral DAPI (Akoya Biosciences, FP1490) and mounted with ProLong Gold Antifade (ThermoFisher Scientific, P36935). Semi-automated image acquisition

(10) Multi-modal image analysis for large-scale cancer tissue studies within IMMUcanCell Rep MethodsSeptember 15, 2025Eling N, Dorier J, Rusakiewicz S, Liechti R, Devanand P, Daniel M, Windhager J, Fernandez BP, Déglise S, Despland L, et al.peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, DAPI,Akoya,Cat#FP1490 Iridium intercalator,Standard Biotools,Cat#201192C DMSO,Sigma Aldrich,Cat#D2650 IFN-ɣ,Roche,Cat#11040596001 Penicillin-Streptomycin,Sigma Aldrich

(11) Utility of [99mTc]Tc-tilmanocept, an immunosuppressive macrophage functional imaging agent in melanoma patients receiving checkpoint inhibitor treatment: a feasibility studyCancer Immunology, ImmunotherapySeptember 6, 2025Sarah Boughdad, Sofiya Latifyan, Margret Schottelius, Michael Blue, Stéphanie Tissot, Christine Geldhof, Julien Costes, John O. Prior, Niklaus Schaefer°C, dilution 1/400), OPAL780. Nuclei were visualized by a final incubation with Spectral DAPI (1/10, FP1490, Akoya Biosciences) for 12 min. Multiplex immunofluorescence images were acquired on PhenoImager allowing

(12) Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implantsSTAR ProtocolsSeptember 1, 2025Shivraj M. Yabaji, Suruchi Lata, Igor Gavrish, Ming Lo, Aoife K. O’Connell, Hans P. Gertje, Colleen E. Thurman, Nicholas A. Crossland, Lester Kobzik, Igor Kramnik45000-344 Fetal bovine serum (FBS),Hyclone,Cat# SH30071.03 10X Spectral DAPI,Akoya Biosciences,Cat# FP1490 Paraformaldehyde solution 4% in PBS,Fisher Scientific,Cat# L-glutamine,Corning,Cat# 25-005-CI Penicillin

(13) P-21 Kinase 1 or 4 Knockout Stimulated Anti-Tumour Immunity Against Pancreatic Cancer by Enhancing Vascular NormalisationInternational Journal of Molecular SciencesAugust 28, 2025Arian Ansardamavandi, Chelsea Dumesny, Yi Ma, Li Dong, Sarah Ellis, Ching-Seng Ang, Mehrdad Nikfarjam, Hong Hecompleting the final cycle of the staining rounds, sections were counterstained with Spectral DAPI (FP1490, Akoya Biosciences, Marlborough, MA, USA) diluted 1:500 in PBS for 5 min to visualise nuclei. The slides

(14) Targeting FSP1 triggers ferroptosis in lung cancerbioRxivAugust 23, 2025Katherine Wu, Alec J Vaughan, Jozef P Bossowski, Yuan Hao, Aikaterini Ziogou, Seon Min Kim, Tae Ha Kim, Mari N Nakamura, Ray Pillai, Mariana Mancini, et al.details). After antibody staining, sections were counterstained with spectral DAPI (Akoya Biosciences, FP1490) and mounted with ProLong Gold Antifade (ThermoFisher Scientific, P36935). Semi-automated image acquisition

(15) Targeting FGFR4 abrogates HNF1A-driven metastasis in pancreatic ductal adenocarcinomaMolecular cancerJuly 29, 2025Crawford KJ, Humphrey KS, Cortes E, Wang J, Kumarasamy V, Wan Y, Long MD, Feigin ME, Witkiewicz AK, Mann KM, et al.Biosystems) secondary antibody, Opal fluorophore (Akoya Biosciences). Spectral DAPI (Akoya Biosciences #FP1490) was manually applied following removal of the samples from the BOND RXm. Processed slides were preserved

(16) Pathogenic strains of a gut commensal drive systemic platelet activation and thromboinflammation in lupus nephritisbioRxivJune 26, 2025Abhimanyu Amarnani, Cristobal F. Rivera, Macintosh Cornwell, Tyler Weinstein, Susan R.S. Gottesman, Cynthia Loomis, Zakia Azad, Andy Lee, Joshua Prasad, Betsy Barnes, et al.as shown in Extended Data Table 4. Slides were counterstained with spectral DAPI (Akoya Biosciences, FP1490) and mounted with ProLong Gold Antifade (ThermoFisher Scientific, P36935). Semi-automated image acquisition

(17) Hypomethylating therapy mitigates acute allograft rejection in a murine lung transplant modelFrontiers in TransplantationJune 23, 2025Kristine M. Yarnoff, William N. Daccarett-Bojanini, Andres F. Villabona-Rueda, Manuel Sollmann, Franco R. D’Alessio, Jeffrey M. Dodd-o(Cat. #7017944001, Roche Diagnostics). Finally, sections were counterstained with spectral DAPI (Cat. #FP1490, Akoya Biosciences) and mounted with Prolong Gold (Cat. #P36930, ThermoFisher Scientific). Slides were

(18) Epithelial GREMLIN1 disrupts intestinal epithelial-mesenchymal crosstalk to induce a wnt-dependent ectopic stem cell niche through stromal remodellingNature communicationsJune 4, 2025Mulholland EJ, Belnoue-Davis HL, Valbuena GN, Gunduz N, Ligeza A, Lin M, Biswas S, Gil Vasquez E, Omwenga S, Nasreddin N, et al.to each primary antibody being applied. Sections were then incubated for 10 min with spectral DAPI (FP1490, Akoya Biosciences) and the slides mounted with VECTASHIELD Vibrance Antifade Mounting Medium (H-1700-10;

(19) Altered Hepatic Metabolism in Down SyndromebioRxivJune 1, 2025Lauren N. Dunn, Brian F. Niemeyer, Neetha P. Eduthan, Kyndal A. Schade, Katherine A. Waugh, Chrisstopher Brown, Angela L. Rachubinski, Ariel E. Timkovich, David J. Orlicky, Matthew D. Galbraith, et al.cat#ab256559), Smooth Muscle Actin (hepatic stellate cells, CST cat#19245S), and DAPI (cell nuclei, Cat# FP1490 Akoya Biosciences). A whole slide scan was performed at 20x on a Vectra Polaris instrument (Akoya Biosciences),

(20) Chromosomal instability shapes the tumor microenvironment of esophageal adenocarcinoma via a cGAS–chemokine–myeloid axisbioRxivMay 25, 2025Bruno Beernaert, Rose L Jady-Clark, Parin Shah, Erik Ramon-Gil, Nora M Lawson, Zack D Brodtman, Somnath Tagore, Frederik Stihler, Alfie S Carter, Shannique Clarke, et al.outlined in Supplementary Table 8. Sections were counterstained with spectral DAPI (Akoya Biosciences, FP1490) and mounted with VECTASHIELD Vibrance Antifade Mounting Medium (Vector Laboratories, H-1700–10). Whole

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Spectral DAPI from Akoya Biosciences, a Quanterix company

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Akoya Biosciences, a Quanterix company

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Spectral DAPI from Akoya Biosciences, a Quanterix company

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