EveryBlot Blocking Buffer from Bio-Rad

Product Specs
ItemEveryBlot Blocking Buffer
CompanyBio-Rad
Price Supplier Page View Company Product Page
Catalog Number12010020 / 12010947
Quantity50 ml or 500 ml

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

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Website: http://discover.bio-rad.com

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(1) Cellular and subcellular localization of Rab10 and phospho-T73 Rab10 in the mouse and human brainActa Neuropathol CommunDecember 18, 2023Vijay Singh, Marissa A. Menard, Geidy E. Serrano, Thomas G. Beach, Hien T. Zhao, Alexis Riley-DiPaolo, Nitya Subrahmanian, Matthew J. LaVoie, Laura A. Volpicelli-Daleymilk in TBS room temperature. pRab10 blots were blocked using “Every Blot” blocking buffer (BioRad 12010020). After blocking, blots were incubated at 4 °C overnight with primary antibodies in blocking buffer.

(2) Mass isolation of staged Drosophila pupal intestines for analysis of protein ubiquitylationSTAR ProtocolsDecember 1, 2023Ruoxi Wang, Panagiotis D. Velentzas, Eric H. BaehreckeWest Atto ultimate sensitivity substrate,Thermo Scientific,A38555 EveryBlot blocking buffer,Bio-Rad,12010020 Pierce BCA protein assay kits,Thermo Scientific,23225 "Blotto, non-fat dry milk",Santa Cruz Biotechnology,sc-2324 10×

(3) Combined Computational–Biochemical Approach Offers an Accelerated Path to Membrane Protein SolubilizationJournal of Chemical Information and ModelingNovember 27, 2023Mariah R. Pierce, Jingjing Ji, Sadie X. Novak, Michelle A. Sieburg, Shivangi Nangia, Shikha Nangia, James L. Houglandstandard protocol (25 V, 30 min). The PVDF membrane was blocked with EveryBlot Blocking Buffer (BioRad;#12010020) for 30 min–2 h. The membrane was incubated in 1:1000 1° MBOAT4 polyclonal antibody (Cayman Chemical

(4) Plant carbonic anhydrase-like enzymes in neuroactive alkaloid biosynthesisNatureNovember 8, 2023Ryan S. Nett, Yaereen Dho, Chun Tsai, Daria Passow, Jaime Martinez Grundman, Yun-Yee Low, Elizabeth S. SattelyTrans-Blot semidry transfer system (BIO-RAD). Blots were blocked in EveryBlot blocking buffer (BIO-RAD 12010020) for more than 5 min at room temperature and incubated with mouse anti-His (Genscript A00186) at 0.1

(5) Luteolin inhibits GPVI-mediated platelet activation, oxidative stress, and thrombosisFrontiers in PharmacologyOctober 31, 2023Yujia Ye, Lihong Yang, Min Leng, Qian Wang, Jiankui Wu, Wen Wan, Huawei Wang, Longjun Li, Yunzhu Peng, Shengjie Chai, et al.and transferred to membranes. The PVDF membranes were blocked in EveryBlot Blocking buffer (Cat No. 12010020; Bio-Rad, Hercules, CA, United States) for 10 min. Then, 1:1,000 primary antibodies were incubated overnight

(6) PUF-8, a C. elegans ortholog of the RNA-binding proteins PUM1 and PUM2, is required for robustness of the cell death fateDevelopmentOctober 1, 2023Jimei Xu, Yanwen Jiang, Ryan Sherrard, Kyoko Ikegami, Barbara Conradttransferred to a polyvinylidene fluoride (PVDF) membrane and blocked using a blocking buffer (Bio-Rad, 12010020). Blots were incubated overnight at 4˚C with either Mouse anti-FLAG antibodies (M2 clone, Sigma F1)

(7) Best practices in assessing cardiac protein O-GlcNAcylation by immunoblotAmerican Journal of Physiology-Heart and Circulatory PhysiologyOctober 1, 2023Luyun Zou, Dingguo Zhang, Chae-Myeong Ha, Adam R. Wende, John C. Chathamcasein, and BSA; although, other commercial agents are available such as EveryBlot from Bio-Rad (Cat. No. 12010947) and BLOTTO from Thermo Fisher Scientific (Cat. No. 37530). In much earlier studies, we found that in

(8) Functional inhibition of the RNA‐binding protein HuR sensitizes triple‐negative breast cancer to chemotherapyMolecular OncologyOctober 1, 2023Lanjing Wei, Qi Zhang, Cuncong Zhong, Lily He, Yuxia Zhang, Ahlam M. Armaly, Jeffrey Aubé, Danny R. Welch, Liang Xu, Xiaoqing Wu(LI‐COR Biosciences, cat# 927‐50000) overnight at 4 °C, or with the EveryBlot Blocking Buffer for (Bio‐Rad, cat# 12010020) 30 min at room temperature. Probed the membrane with a primary antibody followed by a fluorescence‐labeled/HRP‐conjugated

(9) Systemic gene therapy using an AAV44.9 vector rescues a neonatal lethal mouse model of propionic acidemiaMolecular Therapy - Methods & Clinical DevelopmentSeptember 14, 2023Randy J. Chandler, Giovanni Di Pasquale, Eun-Young Choi, David Chang, Stephanie N. Smith, Jennifer L. Sloan, Victoria Hoffmann, Lina Li, John A. Chiorini, Charles P. VendittiTrans-Blot Turbo Transfer Pack (catalog number 1704156, Bio-Rad). EveryBlot Blocking Buffer (catalog number 12010020, Bio-Rad) was used for blocking and antibody hybridization. The following antibodies were used for detection

(10) Supplementation with Vitamin D3 Protects against Mitochondrial Dysfunction and Loss of BDNF-Mediated Akt Activity in the Hippocampus during Long-Term Dexamethasone Treatment in RatsInternational Journal of Molecular SciencesSeptember 11, 2023Daria Korewo-Labelle, Mateusz Jakub Karnia, Dorota Myślińska, Jan Jacek Kaczor0.2 μm were used for semi-dry transfer. The membranes were blocked with Every Blot Blocking Buffer (12010020; BioRad, CA, USA) or 5% nonfat milk in TBST (for the determination of PGC-1α). The primary antibodies

(11) Impaired 1,25-dihydroxyvitamin D3 action underlies enthesopathy development in the Hyp mouse model of X-linked hypophosphatemiaJCI InsightSeptember 8, 2023Rakshya Rana, Jiana T. Baker, Melissa Sorsby, Supriya Jagga, Shreya Venkat, Shaza Almardini, Eva S. Liusubjected to Western blot analysis. Membranes were blocked in EveryBlot blocking buffer (Bio-Rad; catalog 12010020) and then incubated with primary Abs against p-SMAD1/5/9 (1:750; Cell Signaling Technology; catalog

(12) MC1R signaling through the cAMP-CREB/ATF-1 and ERK-NFκB pathways accelerates G1/S transition promoting breast cancer progressionnpj Precision OncologySeptember 7, 2023Vipin Shankar Chelakkot, Kiara Thomas, Todd Romigh, Andrew Fong, Lin Li, Shira Ronen, Shuyang Chen, Pauline Funchain, Ying Ni, Joshua Arbesmanmembranes (Bio-Rad; Cat. #1620115), blocked with 5% milk or EveryBlot Blocking Buffer (Bio-Rad; Cat. #12010020) for 1 h, and incubated with primary antibodies (overnight at 4 °C, after which they were washed and

(13) Gestational stress decreases postpartum mitochondrial respiration in the prefrontal cortex of female ratsNeurobiology of StressSeptember 1, 2023Erin Gorman-Sandler, Breanna Robertson, Jesseca Crawford, Gabrielle Wood, Archana Ramesh, Olufunke O. Arishe, R. Clinton Webb, Fiona Hollisin Supplementary Fig. 1. Membranes were then blocked with EveryBlot Blocking Buffer (Bio-Rad Cat. #12010020) for 30 min (for 4-HNE and TOM20 AB detection) or 1 h (for OXPHOS AB detection) and then incubated overnight

(14) Spastin locally amplifies microtubule dynamics to pattern the axon for presynaptic cargo deliverybioRxivAugust 9, 2023Jayne Aiken, Erika L. F. Holzbaurstain, membranes were de-stained and blocked for 5 minutes with EveryBlot Blocking Buffer (BIO-RAD; 12010020). Membranes were incubated with spastin primary antibody (Sp 3G11/1; Santa Cruz Biotechnology; sc-53443)

(15) Artesunate and cisplatin synergistically inhibit HNSCC cell growth and promote apoptosis with artesunate‑induced decreases in Rb and phosphorylated Rb levelsOncology reportsAugust 1, 2023Okamoto H, Yoshikawa K, Shimada A, Sano R, Inukai D, Yamanaka S, Suzuki S, Ueda R, Ueda H, Fujimoto Y, et al.membrane (Immobilon-P; RPN2232; EMD Millipore). After blocking with EveryBlot Blocking Buffer (cat. no. 12010020; Bio-Rad Laboratories, Inc.) for 30 min at room temperature, the membrane was incubated overnight at

(16) Determinants of Astrocytic Pathology in Stem Cell Models of Primary TauopathiesbioRxivJuly 29, 2023Kimberly L. Fiock, Jordan Hook, Marco M. Hefti4561036, Bio-Rad) and blotted to PVDF. The membrane was blocked in EveryBlot blocking buffer (Cat No. 12010020, Bio-Rad) for 10 minutes at room temperature, then HT7 was diluted 1:5000 in EveryBlot and incubated

(17) Exosomal miR-184 in the aqueous humor of patients with central serous chorioretinopathy: a potential diagnostic and prognostic biomarkerJournal of NanobiotechnologyJuly 28, 2023Jee Myung Yang, Soo Jin Kim, Seongyeol Park, Wonyung Son, Anna Kim, Junyeop Leeafter electrophoresis. The blotted membrane was then blocked using EveryBlot Blocking Buffer (BioRad, #12010020, US). The antibodies used for detection were rabbit anti-STC-2 (Abcam, #ab63057, US), and mouse anti-α-tubulin

(18) Toward countering muscle and bone loss with spaceflight: GSK3 as a potential targetiScienceJuly 21, 2023Ryan W. Baranowski, Jessica L. Braun, Briana L. Hockey, Jenalyn L. Yumol, Mia S. Geromella, Colton J.F. Watson, Nigel Kurgan, Holt N. Messner, Kennedy C. Whitley, Adam J. MacNeil, et al.transfer, membranes were blocked in 5% milk in TBST for 60 min or BioRad Every Blot blocking buffer (12010020) for 10 min. Following blocking, the appropriate primary antibody was appropriately diluted and added

(19) SNHG1 opposes quiescence and promotes docetaxel sensitivity in prostate cancerBMC CancerJuly 18, 2023Steven P. Zielske, Wei Chen, Kristina G. Ibrahim, Frank C. Cackowskigel (#4568083, Bio-Rad) and transferred to PVDF. Membrane was blocked in EveryBlot Blocking Buffer (#12010020, Bio-Rad) for 20 min., then incubated in primary antibody diluted in EveryBlot overnight at 4 °C. Blot

(20) Spatiotemporal control of genome engineering in cone photoreceptorsCell & BioscienceJune 28, 2023Nan-Kai Wang, Pei-Kang Liu, Yang Kong, Yun-Ju Tseng, Laura A. Jenny, Nicholas D. Nolan, Nelson Chen, Hung-Hsi Wang, Chun Wei Hsu, Wan-Chun Huang, et al.to nitrocellulose membranes (Bio-Rad). After blocking the membrane with EveryBlot blocking buffer (12010020; Bio-Rad), the primary antibodies (anti–ARR3, 1:2000, AB15282, Sigma-Aldrich; anti–M-opsin, 1:1000,

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1/27/2023Best Universal Blocking Buffer for Western Blot Regina SolomonThis is the best blocking buffer that I have had the pleasure of using for my western blots. I first started with 5% milk in TBST, but quickly realized that was not the best method to detect ... Read Review

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